Natural DNP-binding immunoglobulins and antibody multispecificity.

abstract：:Aggregation of therapeutic proteins is a key factor in the generation of unwanted immunogenicity, and can result in reduced serum half-life, neutralization of function and adverse health effects. There is currently little information regarding how aggregates interact with B-cell receptors or cognate antibodies at the ...

journal_title：Molecular immunology

authors： Eyes TJ,Austerberry JI,Dearman RJ,Johannissen LO,Kimber I,Smith N,Thistlethwaite A,Derrick JP

更新日期：2019-01-01 00:00:00

abstract：:An attempt was made to obtain fragments containing the C gamma 2 region by selectively cleaving human Fc fragments prepared from pooled IgG at Met residues using cyanogen bromide. Based on the known locations of Met residues in the Fc regions of human IgG subclasses, fragments between Met 252 and 358, comprising the C...

journal_title：Molecular immunology

authors： Boulware DW,Goldsworthy PD,Nardella FA,Mannik M

更新日期：1985-12-01 00:00:00

abstract：:Programmed cell death protein 1 (PD1) is a cell-surface receptor that plays a vital regulatory role in suppressing inflammatory T cell activity; therefore, it is an ideal target for T cell-redirecting therapies. Here, we describe a cynomolgus macaque model for studying the transfer of PD1-modified T cells. We develope...

journal_title：Molecular immunology

authors： Gao CE,Song Q,Zhang M,Li J,Miao Y,Li Z,Dong J

更新日期：2020-08-01 00:00:00

abstract：:Dense deposit disease (DDD) is strongly associated with the uncontrolled activation of the complement alternative pathway. Factor H (CFH)-deficient (Cfh(-/-)) mice spontaneously develop C3 deposition along the glomerular basement membrane (GBM) with subsequent development of glomerulonephritis with features of DDD, a ...

journal_title：Molecular immunology

authors： Paixão-Cavalcante D,Hanson S,Botto M,Cook HT,Pickering MC

更新日期：2009-06-01 00:00:00

abstract：:The monocyte chemoattractant protein-1 (MCP-1) functions to recruit monocytes and macrophages to areas of inflammation and is a prototypic chemokine subjected to coordinate regulation by immunomodulatory agents. TNF mediated regulation of MCP-1 occurs through a distal regulatory region located 2.5 kb upstream of the t...

journal_title：Molecular immunology

authors： Kumar SN,Boss JM

更新日期：2000-08-01 00:00:00

abstract：:Mannan-binding lectin (MBL) is a plasma protein of the innate immune system with the ability to initiate antimicrobial and inflammatory actions. MBL deficiency is common. More than 10% of the general population may, depending on definition, be classified as MBL deficient, underlining the redundancy of the immune syste...

journal_title：Molecular immunology

authors： Thiel S,Frederiksen PD,Jensenius JC

更新日期：2006-01-01 00:00:00

abstract：:Syngeneic polyclonal antibodies were elicited to an affinity labeled high affinity (2-3 X 10(10) M-1) anti-fluorescein murine IgG2a monoclonal antibody. Hyperimmune ascites fluid was tested for reactivity with homologous liganded, affinity labeled and non-liganded Fab fragments derived from the high affinity antibody....

journal_title：Molecular immunology

authors： Voss EW Jr,Miklasz SD,Petrossian A,Dombrink-Kurtzman MA

更新日期：1988-08-01 00:00:00

abstract：:Leprosy is a bacterial disease caused by M. leprae. Its clinical spectrum reflects the host's immune response to the M. leprae and provide an ideal model to investigate the host pathogen interaction and immunological dysregulation. Tregs are high in leprosy patients and responsible for immune suppression of the host b...

journal_title：Molecular immunology

authors： Tarique M,Saini C,Naqvi RA,Khanna N,Sharma A,Rao DN

更新日期：2017-03-01 00:00:00

abstract：BACKGROUND:Complement component C5-derived C5a locally generated in the brain has been shown to protect against glutamate-induced neuronal apoptosis and beta-amyloid (Aβ) toxicity, but the mechanism is not clear. In this study, we tested the hypothesis that C5a influences upstream signal transduction pathways associate...

journal_title：Molecular immunology

authors： Gong B,Pan Y,Zhao W,Knable L,Vempati P,Begum S,Ho L,Wang J,Yemul S,Barnum S,Bilski A,Gong BY,Pasinetti GM

更新日期：2013-12-01 00:00:00

abstract：:A high affinity C5 convertase is generated when a C3 convertase deposits additional C3b molecules on and around itself thereby switching the substrate specificity of C3 convertase from C3 to C5. In the present study the role of the additional C3b molecules in influencing the regulation of classical pathway C5 converta...

journal_title：Molecular immunology

authors： Rawal N,Pangburn MK

更新日期：2007-02-01 00:00:00

abstract：:We have previously developed a number of monoclonal antibodies (Mabs) that bind to protamine. One of these antibodies, Hup1N, binds to human protamine 1 but not to protamine 2. In contrast, Mab HupA binds both protamine 1 and protamine 2. The epitopes for these two Mabs were observed to overlap, and were localized to ...

journal_title：Molecular immunology

authors： Stanker LH,Wyrobek A,McKeown C,Balhorn R

更新日期：1993-12-01 00:00:00

abstract：:Normal rat IgG-Sepharose binds the same two receptors from solubilized rat basophilic leukemia (RBL) cells as are bound by rat IgE-Sepharose. These receptors have previously been designated R and H, and have apparent mol. wts of 45,000 and 55,000, respectively. Inhibition studies indicate that, although soluble deaggr...

journal_title：Molecular immunology

authors： Kepron MR,Conrad DH,Froese A

更新日期：1982-12-01 00:00:00

abstract：:Forty different PCR clones encoding a llama variable heavy chain domain were analysed. The majority of these clones are derived from heavy-chain antibody cDNA in which the entire CH1 exon is absent. It appears from the amino acid within the VHH framework 1 and 3 that all the llama clones belong to the VH III family. H...

journal_title：Molecular immunology

authors： Vu KB,Ghahroudi MA,Wyns L,Muyldermans S

更新日期：1997-11-01 00:00:00

abstract：:Systemic lupus erythematosus (SLE) is a typical autoimmune disease with unclear etiology. Aberrant self-DNA recognition has been proven critical for the initiation of excessive immune responses in lupus. Consistently, we previously reported that a sort of self-DNA, activated lymphocyte-derived DNA (ALD-DNA) could pote...

journal_title：Molecular immunology

authors： Li X,Yue Y,Zhu Y,Xiong S

更新日期：2015-05-01 00:00:00

abstract：:Toll-like receptors (TLR) are expressed on the surface or intracellularly by dendritic cells (DC) and recognize specifically different pathogen-associated molecular patterns (PAMPs). Increasing evidence suggests that TLR expressed by DC can cooperate to synergize their functions. Here, we describe the cooperation of T...

journal_title：Molecular immunology

authors： Theiner G,Rössner S,Dalpke A,Bode K,Berger T,Gessner A,Lutz MB

更新日期：2008-01-01 00:00:00

abstract：:The C-terminal domain of protein 4.1G was identified to interact with the cytosolic tail of the high affinity IgG receptor, Fc gamma RI, in yeast two-hybrid screens. Proteins of the 4.1 family have previously been found to mediate receptor/cytoskeleton interactions. In the study presented here, we show an alternativel...

journal_title：Molecular immunology

authors： Beekman JM,Bakema JE,van der Poel CE,van der Linden JA,van de Winkel JG,Leusen JH

更新日期：2008-04-01 00:00:00

abstract：:Major histocompatibility complex (MHC) class II molecules are cell surface glycoproteins and are known to display processed antigens on the surface of antigen presenting cells (APC). Within the APC, the loading of processed antigenic peptides to MHC class II molecules is known to take place in the endosomal compartmen...

journal_title：Molecular immunology

authors： Mukku PV,Passmore D,Phan D,Nag B

更新日期：1995-06-01 00:00:00

abstract：:The effectiveness of immunotherapy targeting the immune checkpoint PD-L1/PD-1 pathway highlights importance of elucidating the regulatory mechanisms of PD-L1 expression in cancer cells. Previous studies demonstrate that oncogene MYC up-regulates PD-L1 expression in lymphomas. In the present study, we investigated the ...

journal_title：Molecular immunology

authors： Zou J,Zhuang M,Yu X,Li N,Mao R,Wang Z,Wang J,Wang X,Zhou H,Zhang L,Shi Y

更新日期：2018-09-01 00:00:00

abstract：:The membrane anchored ligand ephrinB2 belongs to the broad Eph/ephrin system and is able to activate different Eph receptors. The Eph receptors belong to the huge group of receptor-tyrosine kinases. Eph receptors as well as their corresponding ephrin ligands are cell-membrane attached proteins. Therefore, direct cell-...

journal_title：Molecular immunology

authors： Poitz DM,Ende G,Stütz B,Augstein A,Friedrichs J,Brunssen C,Werner C,Strasser RH,Jellinghaus S

更新日期：2015-12-01 00:00:00

abstract：:Human C3b as well as native C3 were found to bind to solid phase human and rabbit IgG. Haemolytically active C3 had significantly higher binding capacity to IgG than the C3b fragment. Inhibition experiments proved that C3 and C3b have common binding sites on the Fab and Fc part of the IgG molecule but the character of...

journal_title：Molecular immunology

authors： Kulics J,Rajnavölgyi E,Füst G,Gergely J

更新日期：1983-08-01 00:00:00

abstract：:We have previously used immunoelectron microscopy (IEM) to generate a three-dimensional map of idiotypic (Id) and isotypic epitopes on the Fab arms of HGAC 39 (Roux et al., 1987, Proc. natn. Acad. Sci. U.S.A. 84, 4984-4988), a mouse IgG3 monoclonal antibody (Mab). In this report, we analyse the geometry of the various...

journal_title：Molecular immunology

authors： Roux KH,Greenspan NS

更新日期：1994-06-01 00:00:00

abstract：:Macrophages play an important role both in innate and adaptive immune responses. Treatment with interferon (IFN) γ together with lipopolysaccharide (LPS) activates pro-inflammatory macrophages which secrete various pro-inflammatory cytokines including IL-12. IL-12 promotes a Th1 type immune response by directly contro...

journal_title：Molecular immunology

authors： Boonyatecha N,Sangphech N,Wongchana W,Kueanjinda P,Palaga T

更新日期：2012-07-01 00:00:00

abstract：:C-reactive protein (CRP) is a pattern recognition molecule that binds several microbial and host ligands. Ligand-bound CRP activates the complement system via the classical pathway. Previously, we identified human complement factor H-related protein 4 (CFHR4), a member of the factor H protein family, as a CRP binding ...

journal_title：Molecular immunology

authors： Hebecker M,Okemefuna AI,Perkins SJ,Mihlan M,Huber-Lang M,Józsi M

更新日期：2010-03-01 00:00:00

abstract：:The KH type splicing regulatory protein (KSRP) is a nucleic acid binding protein, which negatively regulates the stability and/or translatability of many mRNA species encoding immune-relevant proteins. As KSRP is expressed in immune cells including T and B cells, neutrophils, macrophages and dendritic cells, we wanted...

journal_title：Molecular immunology

authors： Käfer R,Schrick K,Schmidtke L,Montermann E,Hobernik D,Bros M,Chen CY,Kleinert H,Pautz A

更新日期：2017-07-01 00:00:00

abstract：:Interplay between complement factors, regulatory proteins, anaphylatoxins and cytokines could be involved in tendon healing and scar formation. The expression and regulation of complement factors by cytokines or anaphylatoxins are completely unclear in tendon. Hence, the gene expression of the anaphylatoxin receptors ...

journal_title：Molecular immunology

authors： Busch C,Girke G,Kohl B,Stoll C,Lemke M,Krasnici S,Ertel W,Silawal S,John T,Schulze-Tanzil G

更新日期：2013-04-01 00:00:00

abstract：:Immunoglobulins (Ig) require correct folding and assembly of both heavy (H) and light (L) chains to form a functional H2L2 dimer that is secreted from plasma cells. This process is dependent upon the endoplasmic reticulum (ER) chaperone BiP, which targets improperly, folded or assembled Ig molecules for degradation. W...

journal_title：Molecular immunology

authors： McLean GR,Torres M,Trotter B,Noseda M,Bryson S,Pai EF,Schrader JW,Casadevall A

更新日期：2005-05-01 00:00:00

abstract：:We have previously noted that a specific amino acid sequence could form the second framework region of human, mouse and rabbit immunoglobulin light chains, suggesting that this sequence has been preserved for 80 million years. Through divergent evolution, each species has acquired a different set of framework region s...

journal_title：Molecular immunology

authors： Wu TT,Kabat EA

更新日期：1992-09-01 00:00:00

abstract：:The Fc region of IgG antibodies is crucial for binding to Fc receptors expressed on the surfaces of various immune leukocytes and eliciting therapeutic effector functions such as clearance of antibody-opsonized tumor cells. Despite abrogated Fc gamma receptor (FcγR) binding and therapeutic effector function in the abs...

journal_title：Molecular immunology

authors： Yoon HW,Jo M,Ko S,Kwon HS,Lim CS,Ko BJ,Lee JC,Jung ST

更新日期：2019-10-01 00:00:00

abstract：:Although the beneficial effects of probiotics on wide variety of diseases have been shown, little is known about how probiotics modulate the immune system. In this study we elucidated the underlying mechanisms how Lactobacillus casei (L. casei) protects against rheumatoid arthritis (RA) progression by investigating th...

journal_title：Molecular immunology

authors： So JS,Kwon HK,Lee CG,Yi HJ,Park JA,Lim SY,Hwang KC,Jeon YH,Im SH

更新日期：2008-05-01 00:00:00

abstract：:Earlier studies, which provided indirect evidence for the involvement of the C gamma 2 domain of human immunoglobulin G (IgG) in human immunoglobulin G (IgG) in human monocyte binding, have been extended to further localise the site of interaction on human IgG. A number of IgGs from several different species and fragm...

journal_title：Molecular immunology

authors： Woof JM,Partridge LJ,Jefferis R,Burton DR

更新日期：1986-03-01 00:00:00