Abstract：
:Toll-like receptors (TLR) are expressed on the surface or intracellularly by dendritic cells (DC) and recognize specifically different pathogen-associated molecular patterns (PAMPs). Increasing evidence suggests that TLR expressed by DC can cooperate to synergize their functions. Here, we describe the cooperation of TLR9 and TLR4 triggering of murine bone marrow derived DC by CpG oligonucleotides and LPS, respectively. The simultaneous DC stimulation of LPS and CpG showed additive effects on the production of IL-12 but not on other cytokines, such as TNF, IL-6 or IL-10. CpG pretreatment before LPS induced five times more IL-12p40 and IL-12p70 production by DC, whereas LPS pretreatment before CpG showed no effect. The optimal time interval between CpG and LPS treatment was 4h and the synergistic effects were dependent on myeloid differentiation factor 88 (MyD88) but independent from the DNA backbone and did not mediate by nucleosome remodeling. The stimulatory effect could be further enhanced by addition of IFN-gamma but not anti-CD40 antibodies. These data show, that TLR4 and TLR9 can cooperate to increase selectively IL-12 production by DC.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Theiner G,Rössner S,Dalpke A,Bode K,Berger T,Gessner A,Lutz MBdoi
10.1016/j.molimm.2007.02.021subject
Has Abstractpub_date
2008-01-01 00:00:00pages
244-52issue
1eissn
0161-5890issn
1872-9142pii
S0161-5890(07)00079-Xjournal_volume
45pub_type
杂志文章abstract：:We previously demonstrated that some fish have unique response in the form of reactive oxygen species (ROS) production. In the present study, we cloned and sequenced the full-length cDNAs for carp (Cyprinus carpio) phagocyte NADPH oxidase components: gp91phox, p22phox, p47phox, p67phox and p40phox. These amino acid se...
journal_title：Molecular immunology
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abstract：:Fibroblast-like synoviocyte (FLS) is the ultimate effectual cells in the pathogenesis of rheumatoid arthritis (RA). The current study was undertaken to investigate whether a proliferation-inducing ligand (APRIL) mediates the function of FLS in an animal model of RA. Rat adjuvant-induced arthritis (AA) was induced by i...
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pub_type： 杂志文章
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2007-07-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(97)00024-2
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doi：10.1016/j.molimm.2009.02.021
更新日期：2009-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90055-5
更新日期：1990-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.05.014
更新日期：2009-08-01 00:00:00
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pub_type： 杂志文章
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journal_title：Molecular immunology
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2011-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
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更新日期：2015-12-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90118-0
更新日期：1987-12-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90144-1
更新日期：1987-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90162-q
更新日期：1992-01-01 00:00:00
