Contribution of secondary Igkappa rearrangement to primary immunoglobulin repertoire diversification.


:Abs reactive to DNA and DNA/histone complexes are a distinguished characteristic of primary immunoglobulin repertoires in autoimmune B6.MRL-Faslpr and MRL/MpJ-Faslpr mice. These mice are defective in Fas receptor, which is critical for the apoptosis of autoreactive B cells by an extrinsic pathway. In the present study, we explored the possibility that bone marrow small pre-B and immature B cells from adult B6.MRL-Faslpr mice and MRL/MpJ-Faslpr mice respectively, which contain autoreactive B-cell antigen receptors (BCR) and manifest autoimmune syndromes, exhibit enhanced receptor editing patterns. Indeed, FASlpr pre B and immature B cells were shown to possess more ongoing replacements of non-productive (nP) than productive (P) primary VκJκ rearrangements. Significantly, the P vs nP ratios of these replaced primary rearrangements were 1:2, thus indicating that κ light-chain production appears not to inhibit secondary rearrangements. In addition, we identified multiple atypical rearrangements, such as Vκ cRS (cryptic recombination signals) cleavages. These results suggest that the onset of light chain secondary rearrangements persists similarly as a non-selected mode and independent of BCR autoreactivity during certain developmental windows of bone marrow B cells in lupus-prone mice and control, and leads us to propose the function of secondary, de novo Igκ rearrangements to increase BCR diversity.


Mol Immunol


Molecular immunology


Li S,Liu W,Li Y,Zhao S,Liu C,Hu M,Yue W,Liu Y,Wang Y,Yang R,Xiang R,Liu F




Has Abstract


2016-10-01 00:00:00












  • Alterations in T cell signal transduction by M. leprae antigens is associated with downregulation of second messengers PKC, calcium, calcineurin, MAPK and various transcription factors in leprosy patients.

    abstract::Mycobacterium leprae, the causative agent of leprosy, challenges host defense mechanism by impairing the signal transduction of T cells which leads to downregulation of T cell proliferation, mainly as a consequence of interference with IL-2 production. In this study we sought to identify how soluble forms of M. leprae...

    journal_title:Molecular immunology

    pub_type: 临床试验,杂志文章


    authors: Chattree V,Khanna N,Rao DN

    更新日期:2007-03-01 00:00:00

  • Molecular comparison of mouse Thy-1 and its human homologue.

    abstract::Physicochemical techniques were used to verify previous immunochemical studies showing homology of the human Thy-1 (formerly p25) antigen and the murine Thy-1 or theta antigen. Peptide maps and amino acid compositions showed close similarity between these two proteins; however, they were not identical. These data conf...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Bonewald L,Wang AC,Terry E,Zwerner R,Balch CM,Ades EW

    更新日期:1982-03-01 00:00:00

  • The human antibody repertoire: heavy and light chain variable region gene usage in six alloantibodies specific for human HLA class I and class II alloantigens.

    abstract::Peripheral blood B lymphocytes have been isolated from healthy individuals who were immunized with lymphocytes from HLA-incompatible donors and transformed with Epstein-Barr virus to produce human monoclonal cell lines specific for human HLA molecules. The cell lines have been previously characterized and are known to...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Andris JS,Abraham SR,Pascual V,Pistillo MP,Mantero S,Ferrara GB,Capra JD

    更新日期:1995-10-01 00:00:00

  • The mouse genome contains two expressed intronless retroposed pseudogenes for the sentrin/sumo-1/PIC1 conjugating enzyme Ubc9.

    abstract::The ubiquitin conjugating (ubc) E2 enzyme ubc-9 conjugates the ubiquitin-like peptide sentrin/SUMO-1/PIC1 to target proteins which include the Fas antigen. We show that the mouse genome contains four copies of the ubc-9 gene. These include a structural ubc-9 gene consisting of seven exons which encode a protein identi...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Tsytsykova AV,Tsitsikov EN,Wright DA,Futcher B,Geha RS

    更新日期:1998-11-01 00:00:00

  • From interception to incorporation: degeneracy and promiscuous recognition as precursors of a paradigm shift in immunology.

    abstract::The paper evaluates the role of the concept of degeneracy in current immunology. The historical background of the notion is considered, as well as that of related concepts like 'cross-reactivity' and 'promiscuity'. A distinction is made between degeneracy, as a property of a system and promiscuity, as a property of an...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Parnes O

    更新日期:2004-02-01 00:00:00

  • Cloning and characterization of murine CD6.

    abstract::The lymphocyte antigen CD6 has been extensively studied in humans, but has not been reported in mouse. Here we describe the isolation and characterization of a cDNA clone encoding the murine homologue of the human lymphocyte antigen CD6. Comparison of the predicted amino acid sequence of human and murine CD6 shows tha...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Whitney G,Bowen M,Neubauer M,Aruffo A

    更新日期:1995-01-01 00:00:00

  • Amino acid sequence of a light chain variable region of a human rheumatoid factor of the Wa idiotypic group, in part predicted by its reactivity with antipeptide antibodies.

    abstract::Antipeptide antisera were raised to the second and third complementarity-determining regions of the light chain derived from a human monoclonal IgM (Sie) which had antigammaglobulin activity and belonged to the Wa cross-reactive idiotypic group of human rheumatoid factors, two of whose members (Sie, Wo1) had been prev...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Newkirk M,Chen PP,Carson D,Posnett D,Capra JD

    更新日期:1986-03-01 00:00:00

  • The link between morphology and complement in ocular disease.

    abstract::The complement system is a vital component of the immune-priveliged human eye that is always active at a low-grade level, preventing harmful intraocular injuries caused by accumulation of turnover products and controlling pathogens to preserve eye homeostasis and vision. The complement system is a double-edged sword t...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Mohlin C,Sandholm K,Ekdahl KN,Nilsson B

    更新日期:2017-09-01 00:00:00

  • A new enzymatic method to obtain high-yield F(ab)2 suitable for clinical use from mouse IgGl.

    abstract::Four different proteases were screened for their capability of selectively digesting murine monoclonal IgGl to obtain active F(ab)2. For the screening, a series of five different mouse monoclonal antibodies (IgGl, k) was used, recognizing different tumor-associated antigens and currently used for radioimmunoimaging st...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Mariani M,Camagna M,Tarditi L,Seccamani E

    更新日期:1991-01-01 00:00:00

  • Characterization of three novel beta-defensin antimicrobial peptides in rainbow trout (Oncorhynchus mykiss).

    abstract::An initial bioinformatics investigation followed by cloning and sequencing analysis, has led to the identification of three novel members (omDB-2, omDB-3, omBD-4) of the beta-defensin family in rainbow trout (Oncorhynchus mykiss). The contiguous sequences could be translated to give predicted peptides of 62 (omDB-2), ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Casadei E,Wang T,Zou J,González Vecino JL,Wadsworth S,Secombes CJ

    更新日期:2009-10-01 00:00:00

  • MOTS-c peptide increases survival and decreases bacterial load in mice infected with MRSA.

    abstract::Sepsis is a life-threatening disease characterized by uncontrolled inflammatory responses upon pathogen infections, especially for the antibiotic-resistant strains, such as Methicillin-resistant S. aureus (MRSA). Here we demonstrated that a Mitochondria-derived peptide (MOTS-c) could significantly improve the survival...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Zhai D,Ye Z,Jiang Y,Xu C,Ruan B,Yang Y,Lei X,Xiang A,Lu H,Zhu Z,Yan Z,Wei D,Li Q,Wang L,Lu Z

    更新日期:2017-12-01 00:00:00

  • CD55 in rat male reproductive tissue: differential expression in testis and expression of a unique truncated isoform on spermatozoa.

    abstract::CD55 is a key regulator of complement activation, expressed on most tissues and cells in man and other mammals. In the rat, alternative splicing in the gene encoding CD55 yields GPI-anchored (GPI-CD55) and transmembrane (TM-CD55) forms. Published Northern blot analysis indicated that while GPI-CD55 was broadly express...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Mizuno M,Donev RM,Harris CL,Morgan BP

    更新日期:2007-03-01 00:00:00

  • The effect of alternate substrates and substrate concentration on the antibody-mediated inhibition of horseradish peroxidase.

    abstract::Inhibition of the catalytic activity of horseradish peroxidase by rabbit antisera was measured using alternate enzyme substrates. The same general inhibition curve patterns were obtained regardless of the electron donor or hydroperoxide used. In all cases typical biphasic inhibition patterns were found and complete in...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Clark SK Jr,Conroy JM,Harris PJ

    更新日期:1983-12-01 00:00:00

  • Regulation of the murine inducible nitric oxide synthase gene by dexamethasone involves a heterogeneous nuclear ribonucleoprotein I (hnRNPI) dependent pathway.

    abstract::Glucocorticoids down regulate the inducible nitric oxide synthase (iNOS) gene both transcriptionally and post-transcriptionally. The post-transcriptional events are suggested to involve destabilization of the iNOS transcript although the molecular mechanisms for this effect are not known. Recently, our laboratory demo...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Söderberg M,Raffalli-Mathieu F,Lang MA

    更新日期:2007-05-01 00:00:00

  • Maspin inhibits macrophage phagocytosis and enhances inflammatory cytokine production via activation of NF-κB signaling.

    abstract::Maspin (mammary serine protease inhibitor) is a non-inhibitory member of the serine protease inhibitor superfamily and a tumor suppressor in several cancers due to its ability to inhibit cell invasion, angiogenesis, and promote apoptosis. However, its immunomodulatory function remains largely unexplored. Thus, we expl...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Wang Y,Sun L,Song Z,Wang D,Bao Y,Li Y

    更新日期:2017-02-01 00:00:00

  • Engineered hypoallergenic variants of osmotin demonstrate hypoallergenicity with in vitro and in vivo methods.

    abstract::The molecular structure of protein and epitope mapping strategies are required to engineer the epitopes of a protein. In the present study, IgE binding regions of osmotin were identified and mutated to obtain hypoallergenic variant. The three dimensional (3-D) model of osmotin obtained by homology modeling comprised o...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Sharma P,Gaur SN,Goel N,Arora N

    更新日期:2015-03-01 00:00:00

  • Bioactivity studies of rainbow trout (Oncorhynchus mykiss) interleukin-6: effects on macrophage growth and antimicrobial peptide gene expression.

    abstract::Interleukin-6 (IL-6) is a pleiotropic cytokine that regulates hematopoiesis, inflammation, immune responses and bone homeostasis in mammals. Fish IL-6 has been cloned in recent years but to date no functional studies have been reported. Thus, in this paper we present for the first time in fish the functional character...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Costa MM,Maehr T,Diaz-Rosales P,Secombes CJ,Wang T

    更新日期:2011-09-01 00:00:00

  • Primed for success: Oyster parents treated with poly(I:C) produce offspring with enhanced protection against Ostreid herpesvirus type I infection.

    abstract::The Pacific oyster (Crassostrea gigas) is farmed globally. Ostreid herpesvirus (OsHV-1) causes severe mortalities of farmed C. gigas. Management of OsHV-1 has proven difficult. Oysters treated with poly(I:C) exhibit enhanced protection (EP) against OsHV-1. This chemical treatment is highly effective, but it is not fea...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Green TJ,Helbig K,Speck P,Raftos DA

    更新日期:2016-10-01 00:00:00

  • Structural analyses define an additional H-2D region class I antigen that is expressed by a variant mouse strain.

    abstract::The genetic complexity of the H-2D region includes haplotype disparities in apparent gene and product number. To probe the genetic basis of this complexity, the products of two independently derived mouse strains (STU and B10.SAA48) that express Dw3 antigens were compared. Serologic, fluorometric and peptide map compa...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Lee DR,Lillehoj EP,Myers NB,Coligan JE,Hansen TH

    更新日期:1986-10-01 00:00:00

  • Tehranolide molecule modulates the immune response, reduce regulatory T cell and inhibits tumor growth in vivo.

    abstract::Tehranolide named as new type of a sesquiterpene lactone with an endoperoxide group which purified from Artemisia diffusa and having similar structure to Artemisinin. Tehranolide is hypothesized to possess effects akin to Artemisinin, which is active against cancer cells. In the present research we emphasized on the d...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Noori S,Taghikhani M,Hassan ZM,Allameha A,Mostafaei A

    更新日期:2010-04-01 00:00:00

  • Stabilization of peptide guinea pig myelin basic protein 72-85 by N-terminal acetylation-implications for immunological studies.

    abstract::Peptide gpMBP72-85, containing amino acids 72-85 of guinea pig myelin basic protein is commonly used to induce experimental autoimmune encephalomyelitis in Lewis rats. The N-terminal glutamine in this peptide can cyclize to pyroglutamic acid, leading to loss of the first MHC anchor for binding to MHC class II. Acetyla...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: de Haan EC,Wauben MH,Wagenaar-Hilbers JP,Grosfeld-Stulemeyer MC,Rijkers DT,Moret EE,Liskamp RM

    更新日期:2004-02-01 00:00:00

  • LEI0258 microsatellite variability and its association with humoral and cell mediated immune responses in broiler chickens.

    abstract::Major histocompatibility complex (MHC) has a profound influence on disease resistance or susceptibility, productivity and important economic traits in chicken. Association of the MHC with a wide range of immune responses makes it a valuable predictive factor for the disease pathogenesis and outcome. The tandem repeat ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Esmailnejad A,Nikbakht Brujeni G,Badavam M

    更新日期:2017-10-01 00:00:00

  • Cloning and molecular characterization of a human recombinant IgG Fab binding to the Tat protein of human immunodeficiency virus type 1 (HIV-1) derived from the repertoire of a seronegative patient.

    abstract::A study aiming at cloning and characterizing natural antibodies to human immunodeficiency virus type 1 (HIV-1) targets is described. In particular, we report the molecular cloning of a Fab molecule binding the HIV-1/Tat protein from a seronegative patient. The Fab was characterized for its binding specificity and inve...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Mancini N,Perotti M,Carletti S,Canducci F,Sampaolo M,Clementi M,Burioni R

    更新日期:2006-03-01 00:00:00

  • Gene structures and promoter characteristics of interferon regulatory factor 1 (IRF-1), IRF-2 and IRF-7 from snakehead Channa argus.

    abstract::Three interferon regulatory factor (IRF) genes, CaIRF-1, CaIRF-2 and CaIRF-7, and their promoters of snakehead (Channa argus) were cloned and characterized. The CaIRF-1 gene consists of ten exons, spans 4.3 kb and encodes a putative peptide of 299 aa. The CaIRF-2 gene consists of nine exons, spans 8 kb and encodes a p...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Jia W,Guo Q

    更新日期:2008-04-01 00:00:00

  • Fibrates upregulate TRB3 in lymphocytes independent of PPAR alpha by augmenting CCAAT/enhancer-binding protein beta (C/EBP beta) expression.

    abstract::Fibrates, which function by binding and activating peroxisome proliferator-activated receptor alpha (PPARalpha), have been used successfully to treat hyperlipidemia and atherosclerosis. Increasing evidence suggests that in addition to their lipid lowering activities these medications also function as immunosuppressive...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Selim E,Frkanec JT,Cunard R

    更新日期:2007-02-01 00:00:00

  • Junctional diversity prevents negative selection of an antigen-specific T cell repertoire.

    abstract::Endogenous mouse mammary tumor proviruses (MMTV; Mtv loci) deletes Vbeta6 expressing T cells in the thymus of Mtv-7(+) DBA/2 (H2(d)) mice through negative selection. We found that in Mtv-7(-) BALB/c (H2(d)) mice, Vbeta6 is a dominant V gene used in T cell responses to an 18 amino acid long peptide antigen: EYKEYAEYAEY...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Kilgannon P,Novak Z,Fotedar A,Singh B

    更新日期:2010-02-01 00:00:00

  • Isolation and characterization of chicken and turkey beta 2-microglobulin.

    abstract::Chicken and turkey beta 2-m were isolated from citrated plasma in sequential use of three chromatographic steps: affinity chromatography, gel filtration chromatography and anion-exchange chromatography. The purified protein was identified as beta 2-m by reaction with a beta 2-m specific monoclonal antibody and by the ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Skjødt K,Welinder KG,Crone M,Verland S,Salomonsen J,Simonsen M

    更新日期:1986-12-01 00:00:00

  • Biosynthesis of the third component of complement by the human monocyte-like cell line, U-937.

    abstract::The human monocyte-like cell line, U-937, was shown to synthesize and secrete C3 by hemolytic assays, radioimmunoassays and metabolic labeling experiments. The daily synthesis of antigenic C3 by unstimulated U-937 cells was low (about 3 ng/10(6) cells/24 hr) over a 3 day period. Induction of the cells to differentiate...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Minta JO,Isenman DE

    更新日期:1987-10-01 00:00:00

  • Fine specificity and sequence of antibodies directed against the ectodomain of matrix protein 2 of influenza A virus.

    abstract::The ectodomain of matrix protein 2 (M2e) has remained remarkably conserved amongst human influenza A viruses and is a target for Abs with protective activity. For these reasons, M2e is being investigated for its potential as a broadly protective influenza A virus vaccine. Here, we report on the fine specificity and se...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Zhang M,Zharikova D,Mozdzanowska K,Otvos L,Gerhard W

    更新日期:2006-07-01 00:00:00

  • Polymorphism in a kappa I primary (AL) amyloid protein (BAN).

    abstract::In an attempt to understand the relationship of amino acid sequence to the formation of primary or multiple myeloma-related amyloid (AL amyloid), we have determined the complete amino acid sequence of amyloid protein BAN. This protein belongs to the kappa I immunoglobulin light chain subgroup and has a polypeptide cha...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Dwulet FE,O'Connor TP,Benson MD

    更新日期:1986-01-01 00:00:00