Abstract：
:Four cell lines producing monoclonal antibodies were obtained by fusion of NS1 myeloma cells with splenocytes of BALB/C mice immunized with only 1 microgram of each staphylococcal enterotoxin A, B, C1 and D by a modified technique of intrasplenic boosting. This procedure was considerably more efficient than the more commonly used intravenous boosting. The antibodies EC-A1, EC-B1, EC-C1 and EC-D1, all of the IgG1 subclass, have high affinities for the corresponding enterotoxins A, B, C1 and D, with dissociation constants of 1.4, 2.8, 1.4 and 1.5 nM respectively; in addition EC-B1 showed a high affinity (2.1 nM) for enterotoxin C1. All these antibodies recognize, by immunoblotting, the homologous purified enterotoxins as well as enterotoxins from the bacterial culture supernatants. A rapid indirect double sandwich ELISA using a pair of antibody preparations was developed, where monospecific monoclonal antibodies were used to coat plastic plates and polyspecific rabbit antibodies were used to detect the enterotoxins under field conditions. These antibodies which are capable of immunoadsorbing the enterotoxins from staphylococcal culture filtrates and from natural fluids such as milk, were used to immunopurify enterotoxins A, C1 and D. The homogeneity and integrity of the affinity purified toxins A, C1 and D was verified by direct automated Edman degradation and yielded single amino terminal sequences which were moderately homologous to those published previously for B and C1 enterotoxins.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Lapeyre C,Kaveri SV,Janin F,Strosberg ADdoi
10.1016/0161-5890(87)90118-0subject
Has Abstractpub_date
1987-12-01 00:00:00pages
1243-54issue
12eissn
0161-5890issn
1872-9142journal_volume
24pub_type
杂志文章abstract：:Aggregation of therapeutic proteins is a key factor in the generation of unwanted immunogenicity, and can result in reduced serum half-life, neutralization of function and adverse health effects. There is currently little information regarding how aggregates interact with B-cell receptors or cognate antibodies at the ...
journal_title：Molecular immunology
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journal_title：Molecular immunology
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journal_title：Molecular immunology
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更新日期：1983-03-01 00:00:00
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journal_title：Molecular immunology
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(96)00088-0
更新日期：1996-11-01 00:00:00
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更新日期：1998-12-01 00:00:00
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journal_title：Molecular immunology
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更新日期：1996-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.molimm.2011.07.007
更新日期：2011-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(96)00048-x
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更新日期：2012-01-01 00:00:00
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journal_title：Molecular immunology
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journal_title：Molecular immunology
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更新日期：2002-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：1989-03-01 00:00:00
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journal_title：Molecular immunology
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2018-01-01 00:00:00
