Abstract:
:White spot syndrome virus (WSSV) has caused great economic damage to shrimp aquaculture. Previous studies have shown that WSSV successfully usurps the immunity system of the host for its own gene regulation. To investigate the role of shrimp high mobility group box (HMGB) proteins in WSSV gene regulation, two Litopenaeus vannamei HMGB genes, LvHMGBa and LvHMGBb, were isolated by rapid amplification of cDNA ends (RACE). Recombinant LvHMGBa/b proteins were present in the nucleus of transfected Drosophila Schneider 2 (S2) cells. Luciferase reporter assays revealed that LvHMGBa/b upregulated the WSSV immediate-early (IE) gene (ie1) in a NF-κB and STAT binding site-dependent manner. GST pull-down assays demonstrated that LvHMGBa/b interacted with L. vannamei Dorsal (LvDorsal) and L. vannamei STAT (LvSTAT), respectively. LvHMGBa was highly expressed in hepatopancreas while HMGBb was highly expressed in stomach, intestine, heart, antennal gland, and epidermis. Moreover, an immune challenge assay demonstrated that the expression of LvHMGBa/b was upregulated by WSSV infection and that both mRNAs reached peak values at 24 h post-infection. To our knowledge, this is the first report that invertebrate HMGB proteins participates in viral gene regulation.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Chen YH,Jia XT,Huang XD,Zhang S,Li M,Xie JF,Weng SP,He JGdoi
10.1016/j.molimm.2010.11.020subject
Has Abstractpub_date
2011-02-01 00:00:00pages
793-9issue
5eissn
0161-5890issn
1872-9142pii
S0161-5890(10)00655-3journal_volume
48pub_type
杂志文章abstract:OBJECTIVE:Spinal cord injury (SCI) is a neurological disease with high incidence and disability. In this paper, we made a foundational study in long non-coding RNA paternally expressed gene 10 (lncRNA PEG10) at PC-12 cells. METHODS:We used CCK8, flow cytometry, migration and invasion assay to detect changes at the cel...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.11.012
更新日期:2020-02-01 00:00:00
abstract::In extension of the hypothesis that an amphipathic alpha helix of Ii (Phe146-Val164) bound to the foreign antigen-presenting site (desetope) of class II MHC molecules through hydrophobic amino acid residues (Phe146, Leu150, Leu153, Met157, Ile160, Val164) which were present in an axial strip along one side of the Ii h...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90068-x
更新日期:1987-10-01 00:00:00
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doi:10.1016/0161-5890(94)90139-2
更新日期:1994-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.10.007
更新日期:2019-12-01 00:00:00
abstract::C-reactive protein (CRP) is an acute-phase protein featuring a homopentameric structure and Ca-binding specificity for phosphocholine (PCh). Expression of CRP is regulated mainly at the transcriptional level with interleukin-6 being the principal inducer of the gene during the acute phase. The crystal structure of CRP...
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pub_type: 杂志文章,评审
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abstract::Egr-1 is an immediate early gene that is rapidly upregulated in response to mitogenic signals induced by antigen receptor crosslinking on murine B lymphocytes. It has been shown that levels of Egr-1 expression are closely correlated with B cell proliferation in several models of B cell activation and tolerance. We com...
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pub_type: 杂志文章
doi:10.1016/0161-5890(93)90463-l
更新日期:1993-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.05.019
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doi:10.1016/j.molimm.2017.06.035
更新日期:2017-10-01 00:00:00
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更新日期:2004-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(83)90169-4
更新日期:1983-12-01 00:00:00
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更新日期:2021-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2013.07.016
更新日期:2014-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(90)90102-6
更新日期:1990-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(96)00014-4
更新日期:1996-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 信件
doi:10.1016/j.molimm.2007.07.005
更新日期:2008-01-01 00:00:00
abstract::Complement per se has been shown to play an important role in demyelinating disease but controversy remains regarding the role of C3 in the development and progression of experimental autoimmune encephalomyelitis (EAE), the animal model for multiple sclerosis. In this study, we used C3(-/-) mice to confirm previous fi...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.02.002
更新日期:2007-05-01 00:00:00
abstract::T-lymphocyte proliferative responses were determined with peripheral blood lymphocytes (PBL), peritoneal exudate lymphocytes (PEL) and lymph node lymphocytes (LNL) of guinea pigs immunized with horse cytochrome c (HCytc). The proliferative response of PBL activated by peptide 81-104 from animals immunized with HCytc w...
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pub_type: 杂志文章
doi:10.1016/0161-5890(88)90115-0
更新日期:1988-08-01 00:00:00
abstract:BACKGROUND:Ethanol feeding in mice activates complement via C1q binding to apoptotic cells in the liver; complement contributes to ethanol-induced inflammation and injury. Despite the critical role of C1q in ethanol-induced injury, the mechanism by which ethanol activates C1q remains poorly understood. Secretory IgM (s...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.02.008
更新日期:2016-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.10.002
更新日期:2011-01-01 00:00:00
abstract::High glucose- induced endothelial dysregulation has been recognized as an initiation of vascular complications in Type 2 diabetes mellitus (T2DM). Anagliptin is a novel licensed dipeptidyl peptidase-4 (DPP-4) inhibitor for the treatment of T2DM. The effects of anagliptin in high glucose- induced endothelial dysfunctio...
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doi:10.1016/j.molimm.2019.01.006
更新日期:2019-03-01 00:00:00
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doi:10.1016/0161-5890(91)90145-a
更新日期:1991-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(85)90052-5
更新日期:1985-12-01 00:00:00
abstract::C-reactive protein (CRP) is a pattern recognition molecule that binds several microbial and host ligands. Ligand-bound CRP activates the complement system via the classical pathway. Previously, we identified human complement factor H-related protein 4 (CFHR4), a member of the factor H protein family, as a CRP binding ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.12.005
更新日期:2010-03-01 00:00:00
abstract::The complement system is a vital component of the immune-priveliged human eye that is always active at a low-grade level, preventing harmful intraocular injuries caused by accumulation of turnover products and controlling pathogens to preserve eye homeostasis and vision. The complement system is a double-edged sword t...
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pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2017.05.028
更新日期:2017-09-01 00:00:00
abstract::Chemokine effects on leukocyte infiltration into the central nervous system (CNS) are key events in the inflammatory processes of neuroimmunologic and neuroinfectious diseases. Because, chemokines may play important roles in proliferation and differentiation of brain cells and in the initiation and progression of CNS ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(02)00019-6
更新日期:2002-05-01 00:00:00
abstract::The immunoregulatory enzyme indoleamine 2,3-dioxygenase (IDO) suppresses T-cell responses and promotes immune tolerance in tumor resistance. A previous study determined that IDO inhibits Vav1 mRNA expression and the activation of Vav1 and its downstream targets in T cells in the guanine exchange factor (GEF)-independe...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.11.018
更新日期:2017-01-01 00:00:00
abstract:OBJECTIVE:To investigate the impact of death-associated protein kinase 1 (Dapk1) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) via p38MAPK/NF-κB pathway. METHODS:Dapk1+/+ and Dapk1-/- mice were randomized into Control, LPS, SB203580 (a p38MAPK pathway inhibitor) + LPS, and PDTC (a NF-κB pathway inhibitor...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.01.014
更新日期:2020-04-01 00:00:00
abstract::The cell-surface receptor ST2L triggers cytokine release by immune cells upon exposure to its ligand IL-33. To study the effect of ST2L-dependent signaling in different cell types, we generated antagonist antibodies that bind different receptor domains. We sought to characterize their activities in vitro using both tr...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.05.019
更新日期:2016-07-01 00:00:00
abstract::Concanavalin A (Con A)section-binding proteins from mouse spleen leukocytes were characterised by two-dimensional electrophoresis of material precipitated, by Con A plus anti-Con A, from lysates of biosynthetically-labelled cells. Although most cell surface (iodinatable) proteins are known to bind Con A, some of the m...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(83)90030-5
更新日期:1983-04-01 00:00:00
abstract::In immune cells, Src-family kinases have been implicated as critical regulators of a large number of intracellular signaling pathways. Studies of gene knockout mice lacking various family members have both confirmed the requirement for these kinases in some pathways and provided surprising insight into their roles in ...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2004.04.010
更新日期:2004-07-01 00:00:00