Identification of chicken cathelicidin-2 core elements involved in antibacterial and immunomodulatory activities.


:Chicken host defense peptide cathelicidin-2 (CATH-2) is known to exert antimicrobial and immunomodulatory activities and consists of two alpha-helices connected by a hinge region. Here we report the biological properties of the separate alpha-helical segments and the importance of the proline residue in the hinge region. Substitution of proline-14 in the CATH-2 hinge region by leucine, but not by glycine, strongly reduced antibacterial and hemolytic activity. Furthermore, substitution by leucine strongly reduced the neutralization of LPS-induced cytokine production and peptide-induced monocyte chemotactic protein-1 (MCP-1) production by human peripheral blood mononuclear cells (PBMCs). This indicates that the hinge region is important for rapid penetration of the bacterial membrane as well as indirect and direct immunomodulatory activities. The highly cationic and amphipathic N-terminal segment (C1-15) exhibited very potent antibacterial activity and fast killing kinetics, while displaying low cytotoxicity towards chicken erythrocytes and PBMCs. The N-terminal and, to a lesser extent, the C-terminal helical regions potently neutralized LPS-induced release of TNFalpha, IL-6 and IL-10 by PBMCs, while IL-8 production was only moderately affected. These results indicate that core elements within mature CATH-2 can be identified that are linked to antibacterial and/or immunomodulatory activities. Further studies may lead to the development of peptide antibiotics with specific properties that can be used for prophylactic and/or therapeutic applications.


Mol Immunol


Molecular immunology


van Dijk A,Molhoek EM,Veldhuizen EJ,Bokhoven JL,Wagendorp E,Bikker F,Haagsman HP




Has Abstract


2009-08-01 00:00:00














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    authors: Gazarian T,Selisko B,Hérion P,Gazarian K

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    authors: Barnett Foster DE,Sjoquist J,Painter RH

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    authors: Holowka D,Metzger H

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    journal_title:Molecular immunology

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    authors: Narayan K,Su KW,Chou CL,Khoruzhenko S,Sadegh-Nasseri S

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    authors: Pisetsky DS,Caster SA

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    abstract::When mouse dendritic cells (DCs) are isolated from tissues, purified and placed in a nutritive culture they die more rapidly than would be expected from their normal turnover in vivo. This can distort culture assays of DC function. We therefore tested several approaches to prolonging DC survival in culture. Of several...

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    authors: Vremec D,Hansen J,Strasser A,Acha-Orbea H,Zhan Y,O'Keeffe M,Shortman K

    更新日期:2015-02-01 00:00:00

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    authors: Hogenkamp A,Herías MV,Tooten PC,Veldhuizen EJ,Haagsman HP

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    abstract::To test the fate of developing B cells with autoreactive receptor components, we studied mice homozygous for a knock-in transgene coding the VH domain of an IgM ssDNA-binding antibody. The transgene has unmutated C57 BL/6 V gene segments. Homozygous knock-in mice developed normal numbers of spleen and bone marrow B ce...

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    authors: Li J,Geissal ED,Li W,Stollar BD

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    abstract::Lewis (LEW) and Wistar Kyoto (WKY) rats of the same major histocompatibility complex (MHC) haplotype (RT.1(l)) display differential susceptibility to adjuvant-induced arthritis (AIA). LEW are susceptible while WKY are resistant to AIA. To gain insights into the mechanistic basis of these disparate outcomes, we compare...

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    abstract::Mannose-binding lectin (MBL) and the ficolins (Ficolin-1, Ficolin-2 and Ficolin-3) are soluble collagen-like proteins that are involved in innate immune defence. They bind sugar structures or acetylated compounds present on microorganisms and on dying host cells and they initiate activation of the lectin complement pa...

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    authors: Garred P,Honoré C,Ma YJ,Munthe-Fog L,Hummelshøj T

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    abstract::The primary structure of a human mu heavy chain (DAG) protein is described. The native protein is a circular decamer with a molecular weight (Mr) of 500 kDa, each decamer being constituted of the constant domains C mu 2, C mu 3 and C mu4 and interlinked by 15 disulfide bridges. At its NH2-terminal each monomeric chain...

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    authors: Mihaesco C,Ferrara P,Guillemot JC,Congy N,Gendron MC,Roy JP,Sizaret PY,Mihaesco E

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  • Memory T cells of patients with abdominal aortic aneurysm differentially expressed micro RNAs 21, 92a, 146a, 155, 326 and 663 in response to Helicobacter pylori and Lactobacillus acidophilus.

    abstract::Regarding the role of micro RNAs (miRNA) in the proliferation and differentiation of T cells as well as the controversy around the role of bacteria in the pathogenesis of abdominal aortic aneurysm (AAA), the effects of Helicobacter pylori (Hp) and Lactobacillus acidophilus (La) were investigated in the induction of mi...

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    abstract::The C-terminal domain of protein 4.1G was identified to interact with the cytosolic tail of the high affinity IgG receptor, Fc gamma RI, in yeast two-hybrid screens. Proteins of the 4.1 family have previously been found to mediate receptor/cytoskeleton interactions. In the study presented here, we show an alternativel...

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    authors: Beekman JM,Bakema JE,van der Poel CE,van der Linden JA,van de Winkel JG,Leusen JH

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  • Cyanogen bromide cleaves Fc fragments of pooled human IgG at both methionine and tryptophan residues.

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    authors: Boulware DW,Goldsworthy PD,Nardella FA,Mannik M

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  • Accessible intrachain disulfide bonds in hybrids of light chains.

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    authors: DiScipio RG

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:Molecular immunology

    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Ross AL,Sale JE

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