Establishment of an ex vivo model of monocytes-derived macrophages differentiated from peripheral blood mononuclear cells (PBMCs) from HIV-1 transgenic rats.

abstract：:The antigenicity of Human Immunodeficiency Virus type 1 (HIV-1) matrix p18 protein was evaluated by analyzing the specificity of anti-p18 antibodies elicited either in HIV-1 infected humans, or in HIV-1 infected or immunized chimpanzees, against a panel of long and short overlapping synthetic peptides [from 12 to 46 a...

journal_title：Molecular immunology

authors： Mabrouk K,Moulard M,Gluckman JC,Romi R,Rochat H,Van Rietschoten J,Bahraoui E

更新日期：1993-04-01 00:00:00

abstract：:We have designed and produced a stable bispecific scFv dimer (bisFv) by non-covalent association of two hybrid VH-VL pairs derived from an anti-neuraminidase antibody (NC10) and an anti-glycophorin antibody (1C3). The bisFv dimer was demonstrated to have binding activity to the two respective target antigens and was e...

journal_title：Molecular immunology

authors： Atwell JL,Pearce LA,Lah M,Gruen LC,Kortt AA,Hudson PJ

更新日期：1996-12-01 00:00:00

abstract：:Many biological properties of immune complexes (IC) depend upon their size. Quasi-elastic light scattering (QLS) was used to measure a mean equivalent hydrodynamic radius (Rh) and variance of the distribution of model IC composed of bovine serum albumin (BSA) as antigen (Ag) and combinations of two or three well-chara...

journal_title：Molecular immunology

authors： Yarmush DM,Murphy RM,Colton CK,Fisch M,Yarmush ML

更新日期：1988-01-01 00:00:00

abstract：:Lactobacilli are probiotic bacteria that are considered to be beneficial in the gastrointestinal tract of humans. Although lactobacilli are well known to alleviate intestinal inflammation, the molecular basis of this phenomenon is poorly understood. In this study, we investigated the effect of Lactobacillus plantarum ...

journal_title：Molecular immunology

authors： Noh SY,Kang SS,Yun CH,Han SH

更新日期：2015-03-01 00:00:00

abstract：OBJECTIVE:Using multiplex microarray-based component resolved diagnosis (CRD) to investigate the allergen sensitization profile of allergic asthma patients in southern China. METHOD:Serum samples from 57 polysensitized mite allergic asthmatic patients in a tertiary referral centre of southern China were tested with mu...

journal_title：Molecular immunology

authors： Hu H,Luo W,Wu Z,Cai C,Huang H,Sun B

更新日期：2019-01-01 00:00:00

abstract：:V(D)J recombination is a mechanism peculiar to the somatic rearrangement of antigen receptor genes. It requires both expression of the RAG-1 and RAG-2 recombinases and accessibility of the substrate to its recombinase and post-cleavage/DNA repair stage. TCR revision is a genetic correction mechanism that changes T cel...

journal_title：Molecular immunology

authors： Lantelme E,Orlando L,Porcedda P,Turinetto V,De Marchi M,Amoroso A,Mantovani S,Giachino C

更新日期：2008-01-01 00:00:00

abstract：:Two recombinant mouse mammary tumor virus (MMTV) subunit vaccines have been constructed, in which linear sequences of the envelope gp 52 glycoprotein (EP3) or the superantigen (SAg) have been fused to single or multiple repeats of a T-cell epitope (P30) from tetanus toxin. Histidine tags or glutathione-S-transferase (...

journal_title：Molecular immunology

authors： Astori M,Kraehenbuhl JP

更新日期：1996-09-01 00:00:00

abstract：:Bifunctional antigens composed of one L-tyrosine-p-azobenzenearsonate (Tyr-ABA) carrier epitope and one dinitrophenyl (DNP) haptenic epitope separated by 6-aminocaproyl or polyprolyl spacers induced weak IgM anti-DNP plaque-forming cell (PFC) responses in the spleens of mice immunized intraperitoneally, without detect...

journal_title：Molecular immunology

authors： Kaymakcalan Z,Nitecki DE,Lewis GK,Goodman JW

更新日期：1984-06-01 00:00:00

abstract：:Antipeptide antisera were raised to the second and third complementarity-determining regions of the light chain derived from a human monoclonal IgM (Sie) which had antigammaglobulin activity and belonged to the Wa cross-reactive idiotypic group of human rheumatoid factors, two of whose members (Sie, Wo1) had been prev...

journal_title：Molecular immunology

authors： Newkirk M,Chen PP,Carson D,Posnett D,Capra JD

更新日期：1986-03-01 00:00:00

abstract：:Heparin and two dextran sulphate preparations with a low or high average molecular mass (M(r) 5000 and 5 x 10(5), respectively) enhanced binding of radioactively labelled complement factor H to the complement protein C3b, coupled to Sepharose 4B, maximally 2.5-4-fold within a polyanion concn range of 12.5-400 microgra...

journal_title：Molecular immunology

authors： Koistinen V

更新日期：1993-02-01 00:00:00

abstract：:The O-antigen of the gram negative bacteria Brucella is composed of an homopolymer of 4,6-dideoxy-4-formamido-alpha-D-mannopyranosyl (or perosamine). Several mAb interact specifically with only the O-antigen of certain Brucella species. Although, many studies show that this specific recognition results mainly from the...

journal_title：Molecular immunology

authors： Laurent TC,Mertens P,Dierick JF,Letesson JJ,Lambert C,De Bolle X

更新日期：2004-03-01 00:00:00

abstract：OBJECTIVE:To investigate the impact of death-associated protein kinase 1 (Dapk1) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) via p38MAPK/NF-κB pathway. METHODS:Dapk1+/+ and Dapk1-/- mice were randomized into Control, LPS, SB203580 (a p38MAPK pathway inhibitor) + LPS, and PDTC (a NF-κB pathway inhibitor...

journal_title：Molecular immunology

authors： Li T,Wu YN,Wang H,Ma JY,Zhai SS,Duan J

更新日期：2020-04-01 00:00:00

abstract：:Systemic lupus erythematosus (SLE) in patients and certain mouse models exhibits a strong sex bias. Additionally, in most patients, increased serum levels of type I interferon (IFN-α) are associated with severity of the disease. Because increased levels of B cell activating factor (BAFF) in SLE patients and mouse mode...

journal_title：Molecular immunology

authors： Panchanathan R,Choubey D

更新日期：2013-01-01 00:00:00

abstract：:We have designed a 39 amino acid peptide mimic of the conformation-dependent main immunogenic region (MIR) of the Torpedo acetylcholine receptor (TAChR) that joins three discontinuous segments of the Torpedo α-subunit, α(1-12), α(65-79), and α(110 - 115) with two GS linkers: This 39MIR-mimic was expressed in E. coli a...

journal_title：Molecular immunology

authors： Trinh VB,Foster AJ,Fairclough RH

更新日期：2014-05-01 00:00:00

abstract：:The cancer immunosurveillance hypothesis has found strong experimental support in recent years. It is believed that cytotoxic lymphocytes are important effectors in this process. PKCtheta plays an essential role in proliferation, activation and survival of these cells, but also proliferation and survival of leukemic T...

journal_title：Molecular immunology

authors： Garaude J,Kaminski S,Charni S,Aguilò JI,Jacquet C,Plays M,Hernandez J,Rodriguez F,Hipskind RA,Anel A,Villalba M

更新日期：2008-07-01 00:00:00

abstract：:Dynamic interactions between major histocompatibility complex (MHC) class I heavy chains and beta2-microglobulin (beta2m) play a critical role in their stability on the cell surface, and their ability to present peptide antigens to CD8+ T-cells. A cursory review of protein sequence homologies and three-dimensional cry...

journal_title：Molecular immunology

authors： Shields MJ,Hodgson W,Ribaudo RK

更新日期：1999-06-01 00:00:00

abstract：:Because of its lethal effects, ease of preparation, and ability to be delivered by aerosolization, ricin has been developed as a lethal weapon by various terrorist groups. When introduced into the pulmonary system of rodents, ricin causes pathological changes in the lung that are known to occur in acute respiratory di...

journal_title：Molecular immunology

authors： Korcheva V,Wong J,Lindauer M,Jacoby DB,Iordanov MS,Magun B

更新日期：2007-04-01 00:00:00

abstract：:The synovial sarcoma X breakpoint (SSX) gene family contains nine members. The SSX proteins are CT (cancer/testis) antigens and can be expressed in many tumor types. T cell immune response against SSX protein can be detected in tumor patients and mice expressing any SSX. Screening predominant protective epitopes might...

journal_title：Molecular immunology

authors： He Y,Mao L,Lin Z,Deng Y,Tang Y,Jiang M,Li W,Jia Z,Wang J,Ni B,Wu Y

更新日期：2008-05-01 00:00:00

abstract：:We previously showed that a variety of amino acid substitutions at positions 58 and 59 in the V(H) CDR2 of an anti-arsonate (Ars) antibody Fab simultaneously resulted in increased or unaltered affinity for Ars and substantially enhanced affinity for DNA. To test the generality of these observations, we generated and c...

journal_title：Molecular immunology

authors： Hande S,Manser T

更新日期：1997-12-01 00:00:00

abstract：:Porcine deltacoronavirus (PDCoV) is an emerging swine coronavirus causing diarrhea and intestinal damage in nursing piglets. Previous work showed that PDCoV infection inhibits type I interferon (IFN) production. To further identify and characterize the PDCoV-encoded IFN antagonists will broaden our understanding of it...

journal_title：Molecular immunology

authors： Liu X,Fang P,Fang L,Hong Y,Zhu X,Wang D,Peng G,Xiao S

更新日期：2019-10-01 00:00:00

abstract：:Discordant xenografts surviving the initial hyperacute rejection phase may be subject to cellular rejection processes mediated by infiltrating leukocytes including T cells, NK cells and monocytes. The stable adhesion of these cell types to endothelial cells is due to the molecular interaction of the integrins VLA-4 an...

journal_title：Molecular immunology

authors： Mueller JP,Giannoni MA,Hartman SL,Elliott EA,Squinto SP,Matis LA,Evans MJ

更新日期：1997-04-01 00:00:00

abstract：:We have previously generated human IgG1 antibodies that were engineered for reduced binding to the classical Fcγ receptors (FcγRI-III) and C1q, thereby eliminating their destructive effector functions (constant region G1Δnab). In their potential use as blocking agents, favorable binding to the neonatal Fc receptor (Fc...

journal_title：Molecular immunology

authors： Stapleton NM,Armstrong-Fisher SS,Andersen JT,van der Schoot CE,Porter C,Page KR,Falconer D,de Haas M,Williamson LM,Clark MR,Vidarsson G,Armour KL

更新日期：2018-03-01 00:00:00

abstract：:MiRNAs are a class of endogenous tiny RNAs that act as inhibitors of translation or promote RNA degradation by duplex-formation within the 3'-UTR of target mRNAs. They play an important role during a wide range of cellular processes by fine-tuning of gene expression. The differentiation of monocytes to macrophages pla...

journal_title：Molecular immunology

authors： Poitz DM,Augstein A,Gradehand C,Ende G,Schmeisser A,Strasser RH

更新日期：2013-12-01 00:00:00

abstract：:The mast cell function-associated antigen (MAFA) is a type II membranal glycoprotein that was first identified on the surface of rat mucosal-type mast cells of the RBL-2H3 line. A C-type lectin domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM) are located in the extracellular and intracellular domain...

journal_title：Molecular immunology

authors： Abramson J,Xu R,Pecht I

更新日期：2002-09-01 00:00:00

abstract：:Emerging evidence has indicated that vascular endothelial cells (ECs) not only form the barrier between blood and the vessel wall but also serve as conditional innate immune cells. Our previous study found that SIRT1, a class III histone deacetylase, inhibits the inflammatory response in ECs. Recent studies revealed t...

journal_title：Molecular immunology

authors： Li Y,Wang P,Yang X,Wang W,Zhang J,He Y,Zhang W,Jing T,Wang B,Lin R

更新日期：2016-09-01 00:00:00

abstract：:Human IgE against helminths is a normal component of the whole protective response elicitesd during infection, when specific IgE to a great number of antigens is produced; however, few of those IgE binding components are actually allergens. In general, considering the strong Th2/IgE responses during helminth infection...

journal_title：Molecular immunology

authors： Caraballo L,Coronado S

更新日期：2018-08-01 00:00:00

abstract：:The ubiquitin conjugating (ubc) E2 enzyme ubc-9 conjugates the ubiquitin-like peptide sentrin/SUMO-1/PIC1 to target proteins which include the Fas antigen. We show that the mouse genome contains four copies of the ubc-9 gene. These include a structural ubc-9 gene consisting of seven exons which encode a protein identi...

journal_title：Molecular immunology

authors： Tsytsykova AV,Tsitsikov EN,Wright DA,Futcher B,Geha RS

更新日期：1998-11-01 00:00:00

abstract：OBJECTIVE:Spinal cord injury (SCI) is a neurological disease with high incidence and disability. In this paper, we made a foundational study in long non-coding RNA paternally expressed gene 10 (lncRNA PEG10) at PC-12 cells. METHODS:We used CCK8, flow cytometry, migration and invasion assay to detect changes at the cel...

journal_title：Molecular immunology

authors： Niu S,Ni Y,Niu T,Gao J

更新日期：2020-02-01 00:00:00

abstract：:The mechanism by which heparin inhibits the alternative complement pathway (ACP) by a fluid-phase activator, CoVF, has been studied. Results presented here indicate that heparin's major (rate-limiting) effect on the fluid-phase activation of the ACP was to potentiate Factor H activity. Such an effect results in a very...

journal_title：Molecular immunology

authors： Boackle RJ,Caughman GB,Vesely J,Medgyesi G,Fudenberg HH

更新日期：1983-11-01 00:00:00

abstract：:Reduced expression of Erythrocyte Complement Receptor 1 (E-CR1) is envisaged to contribute significantly to the pathophysiology of systemic lupus erythematosus (SLE). We determined the levels of CR1 transcript in the neutrophils from 25 untreated patients with active SLE and 25 normal healthy individuals and, studied ...

journal_title：Molecular immunology

authors： Arora V,Mondal AM,Grover R,Kumar A,Chattopadhyay P,Das N

更新日期：2007-03-01 00:00:00