Studies of the antigenic structure of two cross-reacting proteins, pertussis and cholera toxins, using synthetic peptides.

Abstract:

:Peptide fragments of pertussis toxin subunit 1 (PT-S1) have been synthesized in order to investigate their antigenic and immunogenic activity, and to evaluate their possible use as components of a new vaccine. Two peptides (sequence 73-82, EAERAGRGTG and sequence 107-116, YVDTYGDNAG) were selected for their predictable exposure on the surface of the molecule, and a third (8-18, YRYDSRPPEDV) for its homology with the sequence 6-16 of cholera toxin subunit A (CT-A 6-16) (YRADSRPPDEI). Antipeptide polyclonal antibodies produced in rabbits, were tested in different immunoassays for their ability to interact with toxin proteins. All of them proved interactive with recombinant PT-S1 (rPT-S1); CT-A interact not only, as expected, with anti 8-18 antibodies, due to the high homology between the two toxins in this region, but also, unexpectedly, with anti 107-116 antibodies, in spite of the lack of homology of this peptide with the entire CT. We also found a direct cross-reactivity between the two toxins: anti PT and anti rPT-S1 antibodies interacted with CT-A, whereas anti CT antibodies did not recognize PT. Antipertussis antibodies also recognized the peptide 8-18, which therefore represents at least a part of an antigenic determinant of the toxin, while no interaction could be evidenced between anti-cholera antibodies and any of the peptides, thus demonstrating important differences in the antigenic structures of the two toxins. None of the antipeptide antibodies examined showed protective activity against the toxins in a Chinese hamster ovary (CHO) cell test.

journal_name

Mol Immunol

journal_title

Molecular immunology

authors

Presentini R,Perin F,Ancilli G,Nucci D,Bartoloni A,Rappuoli R,Antoni G

doi

10.1016/0161-5890(89)90025-4

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

95-100

issue

1

eissn

0161-5890

issn

1872-9142

journal_volume

26

pub_type

杂志文章
  • Circular Dichroism reveals evidence of coupling between immunoglobulin constant and variable region secondary structure.

    abstract::Antibodies (Ab) are bifunctional molecules with two domains, a constant region (C) that confers effector properties and a variable (V) region responsible of antigen (Ag) binding. Historically the C and V regions were considered to be functionally independent, with Ag specificity being solely determined by the V region...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2010.02.018

    authors: Janda A,Casadevall A

    更新日期:2010-04-01 00:00:00

  • RIP2-beta: a novel alternative mRNA splice variant of the receptor interacting protein kinase RIP2.

    abstract::RIP2/RICK/CARDIAK is a member of the receptor interacting protein kinase (RIP) family. RIP2 promotes NF-kappaB activation as well as activation of the MAPKs JNK, ERK1/2 and p38 MAPK, thereby playing an emergent role in the innate immune response and NOD signaling. Moreover, RIP2 has been shown to interact with the CAR...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2008.11.002

    authors: Krieg A,Le Negrate G,Reed JC

    更新日期:2009-03-01 00:00:00

  • Fibrates upregulate TRB3 in lymphocytes independent of PPAR alpha by augmenting CCAAT/enhancer-binding protein beta (C/EBP beta) expression.

    abstract::Fibrates, which function by binding and activating peroxisome proliferator-activated receptor alpha (PPARalpha), have been used successfully to treat hyperlipidemia and atherosclerosis. Increasing evidence suggests that in addition to their lipid lowering activities these medications also function as immunosuppressive...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2006.06.006

    authors: Selim E,Frkanec JT,Cunard R

    更新日期:2007-02-01 00:00:00

  • Genetic variation in ICOS regulates mRNA levels of ICOS and splicing isoforms of CTLA4.

    abstract::Genetic and functional studies suggest that polymorphism in cytotoxic T lymphocyte-associated antigen-4 (CTLA4) and inducible costimulator (ICOS) genes, both reported to harbour autoimmune susceptibility loci, could regulate the immune activation through affecting their expression and splicing of CTLA4. To address thi...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2006.08.010

    authors: Kaartinen T,Lappalainen J,Haimila K,Autero M,Partanen J

    更新日期:2007-03-01 00:00:00

  • Signal joint formation is inhibited in murine scid preB cells and fibroblasts in substrates with homopolymeric coding ends.

    abstract::During B and T lymphocyte development, immunoglobulin and T cell receptor genes are assembled from the germline V, (D) and J gene segments (Lewis, S.M., 1994. The mechanism of V(D)J joining: lessons from molecular, immunological and comparative analyses. Adv. Immunol. 56, 27-150). These DNA rearrangements, responsible...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/s0161-5890(99)00053-x

    authors: Sun T,Ezekiel UR,Erskine L,Agulo R,Bozek G,Roth D,Storb U

    更新日期:1999-06-01 00:00:00

  • Fever-range hyperthermia inhibits cells immune response to protein-bound polysaccharides derived from Coriolus versicolor extract.

    abstract::The aim of the study was to explore whether fever-range hyperthermia (FRH) might enhance the anticancer and immunoregulatory activities of protein-bound polysaccharides (PBP), a class of fungus derived immunomodifiers used in the cancer adjuvant therapy. Blood lymphocytes and breast cancer cells (MCF-7) were cultured ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2016.10.013

    authors: Pawlikowska M,Jędrzejewski T,Piotrowski J,Kozak W

    更新日期:2016-12-01 00:00:00

  • Complex regulation of human cathelicidin gene expression: novel splice variants and 5'UTR negative regulatory element.

    abstract::Cationic antimicrobial peptides play important roles in host defense, linking innate and adaptive immunity. hCAP18, the only human antimicrobial cathelicidin, consists of a conserved N-terminal cathelin-like domain and a C-terminal peptide, LL-37. Expression is regulated during myeloid differentiation, and tightly con...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2007.04.023

    authors: Elloumi HZ,Holland SM

    更新日期:2008-01-01 00:00:00

  • Correlation between the human and porcine complement system: a small-angle scattering study of cross immunity and methylamine-induced conformational changes of porcine C3 and C4 proteins.

    abstract::The porcine complement proteins C3 and C4 have been isolated and then characterized using small-angle scattering methods. Within the limits of experimental errors, the porcine proteins are virtually identical with the corresponding human proteins as measured in terms of mol. wt, Mr and radius of gyration, R,: Mr(C3) =...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(91)90181-i

    authors: Osterberg R,Malmensten B,Boive T,Nilsson U,Stigbrand T,Mortensen K

    更新日期:1991-09-01 00:00:00

  • Inhibition of horseradish peroxidase activity by specific antibody: determinant specificity of anticatalytic antibodies.

    abstract::Rabbit antisera specific for horseradish peroxidase inhibit the catalytic activity of the enzyme. All antibodies prepared against the holoenzyme react with the peroxidase apoenzyme. However, only a minority (30-45%) of the total antiperoxidase pool cross react with reduced and alkylated apoenzymes. The antibodies inhi...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(82)90366-2

    authors: Conroy JM,Salter RD

    更新日期:1982-05-01 00:00:00

  • Requirement of MEF2D in the induced differentiation of HL60 promyeloid cells.

    abstract::The regulatory role of MEF2 (myocyte enhancer binding factor 2) proteins in nonmuscle tissues has not been well characterized. We examined the expression of MEF2 family members, namely, MEF2A, -B, -C, and -D, in the differentiation of HL60 promyeloid cells and observed the remarkable increase in the expressions of MEF...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/s0161-5890(99)00140-6

    authors: Shin HH,Seoh JY,Chung HY,Choi SJ,Hahn MJ,Kang JS,Choi MS,Han TH

    更新日期:1999-12-01 00:00:00

  • Expression profiling and validation of reference gene candidates in immune relevant tissues and cells from Atlantic salmon (Salmo salar L.).

    abstract::The expression levels of three commonly used housekeeping genes, EF1-alpha, RPS20 and Beta-Actin, were examined in seven different tissues and leucocytes from non-stimulated Atlantic salmon (Salmo salar L.). The tissues analysed by quantitative real-time PCR were gill, liver, intestine, muscle, spleen, head kidney leu...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2005.07.009

    authors: Ingerslev HC,Pettersen EF,Jakobsen RA,Petersen CB,Wergeland HI

    更新日期:2006-03-01 00:00:00

  • The immune response to Epstein-Barr nuclear antigen: conformational and structural features of antibody binding to synthetic peptides.

    abstract::Naturally developing human antibodies to the Epstein-Barr nuclear antigen recognize synthetic peptides containing sequences from the unusual glycine-alanine region of this protein. We tested antibody binding to a series of peptides of from five to 20 amino acids in length. Peptides as small as seven amino acids could ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(84)90114-7

    authors: Rhodes G,Houghten R,Taulane JP,Carson D,Vaughan J

    更新日期:1984-11-01 00:00:00

  • Symmetry of effector function in the immune system network.

    abstract::Symmetry in antibody plus complement mediated killing between a T15 idiotype-bearing monoclonal antibody (HPC-M2) and an anti-T15 monoclonal antibody (B36-82) is demonstrated. The HPC-M2 antibody is also shown to be multispecific in its effector function, since it lyses both phosphorylcholine coated erythrocytes, and ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(83)90083-4

    authors: Cooper-Willis A,Hoffmann GW

    更新日期:1983-08-01 00:00:00

  • Cellular uptake followed by class I MHC presentation of some exogenous peptides contributes to T cell stimulatory capacity.

    abstract::The T cell stimulatory activity of peptides is known to be associated with the cell surface stability and lifetime of the peptide-MHC (pepMHC) complex. In this report, soluble high-affinity T cell receptors (TCRs) that are specific for pepMHC complexes recognized by the mouse CD8+ clone 2C were used to monitor the cel...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2006.11.016

    authors: Brophy SE,Jones LL,Holler PD,Kranz DM

    更新日期:2007-03-01 00:00:00

  • Isolation and partial characterization of bovine and equine factor D.

    abstract::Bovine and equine factor D were purified to apparent homogeneity as evidenced by a single protein staining band on 7.5-17.5% SDS-PAGE slab gels under both reducing and non-reducing conditions. An apparent mol. wt of 15,000 for bovine D and 22,500 for equine D were noted after SDS-PAGE gel analysis of both reduced and ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(84)90141-x

    authors: Blanchard DB,Leid RW

    更新日期:1984-10-01 00:00:00

  • Engineered hypoallergenic variants of osmotin demonstrate hypoallergenicity with in vitro and in vivo methods.

    abstract::The molecular structure of protein and epitope mapping strategies are required to engineer the epitopes of a protein. In the present study, IgE binding regions of osmotin were identified and mutated to obtain hypoallergenic variant. The three dimensional (3-D) model of osmotin obtained by homology modeling comprised o...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2014.10.019

    authors: Sharma P,Gaur SN,Goel N,Arora N

    更新日期:2015-03-01 00:00:00

  • Transcriptome analysis of Traf6 function in the innate immune response of zebrafish embryos.

    abstract::TRAF6 is a key player at the cross-roads of development and immunity. The analysis of its in vivo molecular function is a great challenge since severe developmental defects and early lethality caused by Traf6 deficiency in knock-out mice interfere with analyses of the immune response. In this study we have used a new ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2010.08.011

    authors: Stockhammer OW,Rauwerda H,Wittink FR,Breit TM,Meijer AH,Spaink HP

    更新日期:2010-11-01 00:00:00

  • Immunological relationships among group I and group V allergens from grass pollen.

    abstract::Specific IgE antibodies have been affinity-purified from recombinant grass pollen allergens, and used to identify isoforms of the two major allergens of rye-grass pollen, Lol p I and Lol p V and cross-reactive allergens in other grasses. Lol p I-specific IgE (affinity-purified from the recombinant protein expressed by...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(94)90068-x

    authors: Smith PM,Ong EK,Knox RB,Singh MB

    更新日期:1994-04-01 00:00:00

  • CD40 ligand-triggered human dendritic cells mount interleukin-23 responses that are further enhanced by danger signals.

    abstract::Interleukin (IL)-23 is a heterodimeric cytokine composed of the IL-23-specific subunit p19 and the p40 subunit which also constitutes part of IL-12. IL-23 propagates development of Th17 cells, a novel T cell subset which produces IL-17 but no interferon-gamma or IL-4. For both, IL-23 and IL-23-driven IL-17, a crucial ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2009.12.008

    authors: Sender LY,Gibbert K,Suezer Y,Radeke HH,Kalinke U,Waibler Z

    更新日期:2010-03-01 00:00:00

  • Immunochemical and related interactions with dextrans reviewed in terms of improved structural information.

    abstract::Structurally diverse dextrans from Leuconostoc mesenteroides and related bacteria have been used extensively in fundamental immunochemical studies such as induction and characterization of anti-dextran antibodies, as well as in studies of their interaction with pneumococcal antisera, normal bovine serum, concanavalin ...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审

    doi:10.1016/0161-5890(86)90131-8

    authors: Jeanes A

    更新日期:1986-09-01 00:00:00

  • A CD20 tandem-epitope immunogen elicits antibody in mice that binds murine cell surface CD20 and depletes splenic B cells in vivo.

    abstract::CD20 is an important target for monoclonal antibody therapy of B-cell malignancies and for some autoimmune disorders. Though there is interest in evaluating the induction of active immunity to CD20 in the mouse model, the CD20 extracellular domain (ECD) has significant secondary and tertiary structure which make it di...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2010.01.026

    authors: Oscherwitz J,Gribbin TE,Cease KB

    更新日期:2010-04-01 00:00:00

  • Blocking oncogenic RAS enhances tumour cell surface MHC class I expression but does not alter susceptibility to cytotoxic lymphocytes.

    abstract::Mutations in the RAS family of oncogenes are highly prevalent in human cancer and, amongst its manifold effects, oncogenic RAS impairs the expression of components of the antigen presentation pathway. This allows evasion of cytotoxic T lymphocytes (CTL). CTL and natural killer (NK) cells are reciprocally regulated by ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2013.11.020

    authors: El-Jawhari JJ,El-Sherbiny YM,Scott GB,Morgan RS,Prestwich R,Bowles PA,Blair GE,Tanaka T,Rabbitts TH,Meade JL,Cook GP

    更新日期:2014-04-01 00:00:00

  • Complement control protein factor H: the good, the bad, and the inadequate.

    abstract::The complement system is an essential component of the innate immune system that participates in elimination of pathogens and altered host cells and comprises an essential link between the innate and adaptive immune system. Soluble and membrane-bound complement regulators protect cells and tissues from unintended comp...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审

    doi:10.1016/j.molimm.2010.05.007

    authors: Ferreira VP,Pangburn MK,Cortés C

    更新日期:2010-08-01 00:00:00

  • Magnolol suppresses NF-kappaB activation and NF-kappaB regulated gene expression through inhibition of IkappaB kinase activation.

    abstract::The mis-regulation of nuclear factor-kappa B (NF-kappaB) signal pathway is involved in a variety of inflammatory diseases that leds to the production of inflammatory mediators. Our studies using human U937 promonocytes cells suggested that magnolol, a low molecular weight lignan isolated from the medicinal plant Magno...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2006.12.004

    authors: Tse AK,Wan CK,Zhu GY,Shen XL,Cheung HY,Yang M,Fong WF

    更新日期:2007-04-01 00:00:00

  • Characterization of a murine monoclonal antibody specific for the human P1 blood group antigen.

    abstract::Four monoclonal antibodies (MAbs) directed against the P1 blood group antigen were produced by hybridomas obtained from mouse immunized with turtle-dove avomucoid. One of the MAb (154 IX B6) selected as a blood typing reagent agglutinated native P1 and Pk1 red cells with a high titer but was inactive against native P2...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(87)90089-7

    authors: Bailly P,Chevaleyre J,Sondag D,François-Gérard C,Piquet Y,Vezon G,Cartron JP

    更新日期:1987-02-01 00:00:00

  • A monoclonal antibody (RH1-38) which inhibits multiple systems of cell-mediated cytotoxicity--I. Identification of a novel epitope on a killer cell surface bimolecular complex important in the cytotoxic response.

    abstract::This paper presents the initial characterization of a mouse monoclonal antibody (RH1-38) which blocks, in the absence of complement, three different systems of cell-mediated cytotoxicity. This monoclonal antibody markedly inhibits cytotoxicity mediated by human natural killer cells, a monocyte-like cell [phorbol myris...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(85)90140-3

    authors: Hall RE

    更新日期:1985-07-01 00:00:00

  • Biased dA/dT somatic hypermutation as regulated by the heavy chain intronic iEmu enhancer and 3'Ealpha enhancers in human lymphoblastoid B cells.

    abstract::Somatic hypermutation (SHM) in immunoglobulin gene (Ig) variable (V) regions is critical for the maturation of the antibody response. It is dependent on the expression of activation-induced cytidine deaminase (AID) and translesion DNA polymerases in germinal center B cells as well as Ig V transcription, as regulated b...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2005.10.018

    authors: Komori A,Xu Z,Wu X,Zan H,Casali P

    更新日期:2006-04-01 00:00:00

  • Site A of the MCP-1 distal regulatory region functions as a transcriptional modulator through the transcription factor NF1.

    abstract::The monocyte chemoattractant protein-1 (MCP-1) functions to recruit monocytes and macrophages to areas of inflammation and is a prototypic chemokine subjected to coordinate regulation by immunomodulatory agents. TNF mediated regulation of MCP-1 occurs through a distal regulatory region located 2.5 kb upstream of the t...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/s0161-5890(00)00097-3

    authors: Kumar SN,Boss JM

    更新日期:2000-08-01 00:00:00

  • Effects of sulphated polyanions on functions of complement factor H.

    abstract::Heparin and two dextran sulphate preparations with a low or high average molecular mass (M(r) 5000 and 5 x 10(5), respectively) enhanced binding of radioactively labelled complement factor H to the complement protein C3b, coupled to Sepharose 4B, maximally 2.5-4-fold within a polyanion concn range of 12.5-400 microgra...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(93)90082-m

    authors: Koistinen V

    更新日期:1993-02-01 00:00:00

  • Interleukin-1 Receptor-Associated Kinase-1 (IRAK-1) functionally associates with PKCepsilon and VASP in the regulation of macrophage migration.

    abstract::Macrophage migration is mediated by complex cellular signaling processes and cytoskeleton re-arrangement. In particular, recent advances indicate that the innate immunity signaling process plays a key role in the regulation of macrophage migration. In this report, we have provided evidence demonstrating the involvemen...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2009.12.004

    authors: Gan L,Li L

    更新日期:2010-03-01 00:00:00