Abstract：
:It is well established that aggregation of cell surface immunoglobulin is involved in signal transduction by cells of the immune system. It is less well understood what special properties of these cell surface aggregates are important in initiating the signal cascade. Several authors have proposed that cells respond to the size (Fewtrell and Metzger (1980) J. Immun. 125, 701-710) as well as the stereochemistry (Ortega et al. (1989) Eur. J. Immun. 19, 2251-2256) of receptor aggregates. One approach to arriving at data relevant to this question has been to construct simple bivalent ligands that can bind to surface immunoglobulin. Several authors have suggested that when these bivalent ligands interact with surface immunoglobulin the formation of small stable cyclic complexes is highly favored. In this paper we consider whether it is possible to completely determine the parameters that describe the binding of a bivalent ligand to a bivalent receptor with the available experimental technology. We show that with the appropriate analysis procedure, using a modified equivalent site model, these parameters can be reliably determined from only three experiments even when there is a large amount of ring formation.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Posner RG,Dembo Mdoi
10.1016/0161-5890(94)90160-0subject
Has Abstractpub_date
1994-12-01 00:00:00pages
1439-45issue
18eissn
0161-5890issn
1872-9142journal_volume
31pub_type
杂志文章abstract：:Mouse monoclonal antibody AUK12-20 binds to human IL-6 receptor and inhibits IL-6 functions. It has been humanized by CDR-grafting for therapeutic use. In the design of reshaped human AUK12-20 VL region, the human framework regions (FRs) from the human Bence-Jones protein REI were used. The reshaped human AUK12-20 lig...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90115-5
更新日期：1994-04-01 00:00:00
abstract：:The insect gut participates in initial local immune responses by producing reactive oxygen and nitrogen species as well as anti-microbial peptides to resist pathogenic invasions. Nitric oxide (NO), a signaling and an immune effector molecule synthesized by the enzyme NO synthase (NOS), mediates an early step of the si...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.07.018
更新日期：2020-10-01 00:00:00
abstract：:The host response to intravascular, Gram-negative bacteria includes profound immunologic, hematologic and physiologic changes. Numerous host defense mechanisms are activated by Gram-negative bacteria, including the complement system. Activation of the complement system leads to cleavage of C5 with subsequent generatio...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.10.037
更新日期：2008-04-01 00:00:00
abstract：:The immunoglobulin JHC mu intron was cloned from genomic DNA of a VHa3 rabbit and a 1257 bp sequence which contains conserved enhancer and splice sites was determined. From positions 315 to 1257, there is approximately 72 and 67% similarity to available sequences of man and mouse, respectively (counting gaps as single...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90119-3
更新日期：1989-10-01 00:00:00
abstract：:Cytohesin-1 is a guanine nucleotide exchange factor for ADP ribosylation factor 6 (Arf6) in human blood neutrophils and differentiated PLB-985 neutrophil-like cells. Cytohesin-1 regulates adhesion and the transendothelial migration of monocytes, dendritic cells and T lymphocytes through activation of the β2 integrin L...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.03.018
更新日期：2011-07-01 00:00:00
abstract：:ASF1a (anti-silencing function 1a), an evolutionarily conserved protein and a histone chaperone, is required for a variety of chromatin-mediated cellular processes. However, the function of ASF1a in innate immune response remains unclear. Here, we find that ASF1a is induced in Vesicular Stomatitis Virus (VSV)-infected...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.08.008
更新日期：2016-10-01 00:00:00
abstract：:Inhibition of the catalytic activity of horseradish peroxidase by rabbit antisera was measured using alternate enzyme substrates. The same general inhibition curve patterns were obtained regardless of the electron donor or hydroperoxide used. In all cases typical biphasic inhibition patterns were found and complete in...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90169-4
更新日期：1983-12-01 00:00:00
abstract：:A bovine papilloma virus-derived vector was used to direct the high level expression in mouse C127 cells of three different cDNAs encoding the human interleukin-2 receptor. These were: the previously described cDNA clone isolated from the T-cell lymphoma, HUT-102; a cDNA clone isolated from mitogen-activated, normal p...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90123-9
更新日期：1986-09-01 00:00:00
abstract：:Interplay between complement factors, regulatory proteins, anaphylatoxins and cytokines could be involved in tendon healing and scar formation. The expression and regulation of complement factors by cytokines or anaphylatoxins are completely unclear in tendon. Hence, the gene expression of the anaphylatoxin receptors ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.09.001
更新日期：2013-04-01 00:00:00
abstract：:Malignant cells expressing NKG2D ligands on their cell surface can be directly sensed and killed by NKG2D-bearing lymphocytes. To ensure this immune recognition, accumulating evidence suggests that NKG2D ligands are trafficed via alternative pathways to the cell surface. We have previously shown that the NKG2D ligand ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2015.04.022
更新日期：2015-08-01 00:00:00
abstract：:Meningococcal factor H-binding protein (fHbp) is a promising vaccine antigen. Previous studies described three fHbp antigenic variant groups and identified amino acid residues between 100 and 255 as important targets of variant-specific bactericidal antibodies. We investigated residues affecting expression of an epito...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.02.021
更新日期：2009-05-01 00:00:00
abstract：:Adiponectin can protect against inflammation; one of the mechanisms involves direct, inhibition of macrophages (MΦ). We postulated that adiponectin anti-sense transgenic (AsTg) mice raised in our laboratory are prone to inflammation because of systemic low adiponectin levels. The writhing response to acetic acid was u...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2013.08.015
更新日期：2014-02-01 00:00:00
abstract：:A phosphorylcholine (PC)-binding IgG (Mab2) antibody produced by a hybridoma derived from a BALB/c mouse which had been immunized against Trichinella spiralis was found to bind to the immunizing antigen (TSC) but not to other PC-associated antigens such as pneumococcal antigen (PNC) and PC-conjugated ovalbumin (PC-OVA...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90106-6
更新日期：1994-10-01 00:00:00
abstract：:The p38 mitogen-activated protein kinases (MAPKs) are essential cytoplasmic signal molecules of innate immune pathways that play a vital role in host immune defense responses to pathogenic challenges. In this study, two fish p38 genes (Cip38α and Cip38β) were characterized for the first time from the grass carp Ctenop...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.12.007
更新日期：2020-02-01 00:00:00
abstract：:Chlorophyllin (CHL) was earlier shown to reduce the level of intracellular ROS and apoptosis induced by ionizing radiation and 2,2'-azobis(2-propionimidinedihydrochloride) (AAPH). In the present studies, the effect of CHL on radiation-induced immunosuppression and modulation of immune responses in mice was examined. C...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.02.031
更新日期：2007-01-01 00:00:00
abstract：:Discordant xenografts surviving the initial hyperacute rejection phase may be subject to cellular rejection processes mediated by infiltrating leukocytes including T cells, NK cells and monocytes. The stable adhesion of these cell types to endothelial cells is due to the molecular interaction of the integrins VLA-4 an...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(97)00042-4
更新日期：1997-04-01 00:00:00
abstract：:Consumption of fermentable dietary fibres, such as inulin, or administration of helminth products (e.g. Trichuris suis ova) have independently been shown to alleviate inflammation in vivo. We recently found that dietary inulin and T. suis infection in pigs co-operatively suppressed type-1 inflammatory responses in the...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.03.006
更新日期：2020-05-01 00:00:00
abstract：:Here we present the gene structure and expression data on two Atlantic salmon TNF-alpha genes. Both genes are approximately 2.0kb in length and organized into four exons and three introns. The open reading frame of both genes translates into 246 amino acid putative peptides, being 91.5% identical at the amino acid lev...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.08.015
更新日期：2007-03-01 00:00:00
abstract：:C1q, the recognition molecule of the classical pathway of complement, binds to endothelial cells, leading to cell activation. Mannose-binding lectin (MBL), a recognition molecule of the lectin pathway, is structurally and functionally related to C1q. Therefore, we investigated the interaction of MBL with human umbilic...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.06.015
更新日期：2007-02-01 00:00:00
abstract：:Monoclonal antibodies (MAbs) raised against trout immunoglobulin (Ig) recognize two independent Ig populations differing in the molecular weight of L chains. The trout Ig population recognized by MAb 2H9 represented congruent to 20% of the total Ig and contained L chains congruent to 26 kDa, whereas the trout Ig popul...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90014-b
更新日期：1991-11-01 00:00:00
abstract：:V(D)J recombination is a mechanism peculiar to the somatic rearrangement of antigen receptor genes. It requires both expression of the RAG-1 and RAG-2 recombinases and accessibility of the substrate to its recombinase and post-cleavage/DNA repair stage. TCR revision is a genetic correction mechanism that changes T cel...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.06.153
更新日期：2008-01-01 00:00:00
abstract：:To investigate the interaction of DNA and anti-DNA antibodies in the immune complex disease of systemic lupus erythematosus, the fine specificity of binding of a monoclonal anti-DNA antibody was determined. This antibody, termed Cll, was derived from the fusion of spleen cells from an autoimmune MRL-lpr/lpr mouse with...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90364-9
更新日期：1982-05-01 00:00:00
abstract：:The role of secondary structures and base mutability at different levels of transcription and supercoiling is analyzed in variable region antibody genes VH5, VH94 and VH186.2. The data are consistent with a model of somatic hypermutation in which increasing levels of transcription and secondary structure stability cor...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.05.012
更新日期：2008-08-01 00:00:00
abstract：:Following measles virus (MV) infection, viral peptides are presented to CTL by MHC class I molecules on infected antigen presenting cells at widely different epitope densities. Whereas three MV epitopes (MV-M(211-219), MV-F(438-446) and MV-H(30-38)) derived from different structural proteins occur at regular densities...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(02)00204-3
更新日期：2003-01-01 00:00:00
abstract：:The antigenic structural features of alpha-lactalbumin have been investigated using a radioimmunoassay, peptide inhibition of the quantitative precipitin reaction, and by immunodiffusion analysis after chemical modification of the molecule. Antigenic activity (in rabbits) was localized to several peptic fragments and ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90192-4
更新日期：1982-11-01 00:00:00
abstract：:Cationic antimicrobial peptides play important roles in host defense, linking innate and adaptive immunity. hCAP18, the only human antimicrobial cathelicidin, consists of a conserved N-terminal cathelin-like domain and a C-terminal peptide, LL-37. Expression is regulated during myeloid differentiation, and tightly con...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.04.023
更新日期：2008-01-01 00:00:00
abstract：:Spondyloarthritis is the second most common form of chronic inflammatory arthritis and a unique hallmark of the disease is pathologic new bone formation. Several cytokine pathways have been genetically associated with ankylosing spondylitis (AS), the prototypic subtype of SpA, and additional evidence from human and an...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2013.07.016
更新日期：2014-01-01 00:00:00
abstract：:Topoisomerase cDNA and various fragments thereof generated by the DNA polymerase chain reaction were cloned into plasmid expression vectors (pET series) and the expressed polypeptides were probed with scleroderma sera from seven different patients immunoreactive with topoisomerase I. All sera reacted selectively with ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90145-a
更新日期：1991-04-01 00:00:00
abstract：:When mouse dendritic cells (DCs) are isolated from tissues, purified and placed in a nutritive culture they die more rapidly than would be expected from their normal turnover in vivo. This can distort culture assays of DC function. We therefore tested several approaches to prolonging DC survival in culture. Of several...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.07.011
更新日期：2015-02-01 00:00:00
abstract：:Ovalbumin (OVA) is a major allergen (Gal d II) of hen egg white and is often the cause of hypersensitivity reactions to food. Further knowledge of the antigenic and allergenic epitopes of allergens will provide better treatment of this disease. To analyse these epitopes we produced a panel of monoclonal antibodies (mA...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90055-3
更新日期：1992-10-01 00:00:00
