II. Correlations between secondary structure stability and mutation frequency during somatic hypermutation.

Abstract:

:The role of secondary structures and base mutability at different levels of transcription and supercoiling is analyzed in variable region antibody genes VH5, VH94 and VH186.2. The data are consistent with a model of somatic hypermutation in which increasing levels of transcription and secondary structure stability correlate with the initial formation of successive mutable sites. Encoded differences exist in stem length and the number of GC pairs at low versus high levels of transcription in CDRs. These circumstances simplify the complexities of coordinating mutagenesis by confining this process to each mutable site successively, as they form in response to increasing levels of transcription during affinity maturation.

journal_name

Mol Immunol

journal_title

Molecular immunology

authors

Wright BE,Schmidt KH,Davis N,Hunt AT,Minnick MF

doi

10.1016/j.molimm.2008.05.012

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

3600-8

issue

13

eissn

0161-5890

issn

1872-9142

pii

S0161-5890(08)00192-2

journal_volume

45

pub_type

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