Abstract:
:Here we present the gene structure and expression data on two Atlantic salmon TNF-alpha genes. Both genes are approximately 2.0kb in length and organized into four exons and three introns. The open reading frame of both genes translates into 246 amino acid putative peptides, being 91.5% identical at the amino acid level. The upstream regulatory region of both genes were amplified by inverse PCR and analyzed for putative regulatory binding sites. Variant specific gene-expression both in vitro and in vivo revealed that the two variants are differentially regulated. TNF-alpha2 is the dominant variant throughout the experimental period following LPS stimulation of TO-cells. The picture was different in head kidney tissue following vaccination of Atlantic salmon with an experimental multivalent oil-based vaccine. TNF-alpha2 was the most dominant transcript in the non-stimulated controls and in the early phase. A shift from TNF-alpha2 to TNF-alpha1 was however seen, and from day 12 the TNF-alpha1 was most abundant transcript. Implications of variant specific gene-expression are discussed.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Haugland O,Mercy IS,Romøren K,Torgersen J,Evensen Odoi
10.1016/j.molimm.2006.08.015subject
Has Abstractpub_date
2007-03-01 00:00:00pages
1652-63issue
7eissn
0161-5890issn
1872-9142pii
S0161-5890(06)00543-8journal_volume
44pub_type
杂志文章abstract::Structural similarities between two members of the immunoglobulin superfamily were explored by making chimeric immunoglobulin/CD4 antigen molecules. A crossover in the middle of the originally proposed J kappa homology unit of the first domain of the CD4 molecule was used to construct a chimeric molecule having human ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90002-s
更新日期:1993-06-01 00:00:00
abstract::Several mutations in the CFH gene have been described in non-Shiga-toxin-associated haemolytic uraemic syndrome (non-Stx-HUS), a rare syndrome characterized by haemolytic anaemia, thrombocytopenia and acute renal failure. Mutations in genes encoding other complement regulatory proteins, membrane cofactor protein (CD46...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.08.004
更新日期:2007-03-01 00:00:00
abstract::As an initial step towards the molecular analysis of the murine V kappa locus, a cosmid library from BALB/cJ mouse liver DNA was screened with probes representing 10 V kappa families. Of eight cosmids that were isolated from the initial screen, five contained a single restriction fragment that hybridized to the probes...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(92)90015-p
更新日期:1992-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(85)90072-0
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abstract::The pentacyclic triterpene α,β-amyrin has been previously reported as an effective compound in the treatment of several inflammatory conditions. Recent evidence indicates that α,β-amyrin displayed its effects through interaction with the cannabinoid pathway. We assessed the anti-inflammatory effects of the α,β-amyrin ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2013.01.018
更新日期:2013-07-01 00:00:00
abstract::Sepsis-induced inflammatory damage is a crucial cause of acute kidney injury (AKI), and AKI is an ecumenical fearful complication in approximately half of patients with sepsis. CCAAT/enhancer-binding protein β (C/EBPβ) plays roles in regulating acute phase responses and inflammation. However, the role and mechanism of...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.08.023
更新日期:2020-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.11.018
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Allergen epitope characterization provides valuable information useful for the understanding of proteins as food allergens. It is believed that IgE epitopes in general are conformational, nevertheless, for food allergens known to sensitize through the gastrointestinal tract linear epitopes have been suggeste...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2012.04.002
更新日期:2012-07-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.08.008
更新日期:2016-10-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(03)00102-0
更新日期:2003-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.09.034
更新日期:2009-02-01 00:00:00
abstract:OBJECTIVE:To investigate the impact of death-associated protein kinase 1 (Dapk1) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) via p38MAPK/NF-κB pathway. METHODS:Dapk1+/+ and Dapk1-/- mice were randomized into Control, LPS, SB203580 (a p38MAPK pathway inhibitor) + LPS, and PDTC (a NF-κB pathway inhibitor...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.01.014
更新日期:2020-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(95)00134-4
更新日期:1996-02-01 00:00:00
abstract::Mycobacterium tuberculosis (M.tb) infects 8 million and kills 2.2 million people each year worldwide. M.tb modulates the immune response of the infected individual. Empirically, suppressor carbohydrates (SC) produced by CD8+ T cells in response to M.tb were found to induce a T helper 2 response rather than a protectiv...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.10.022
更新日期:2008-03-01 00:00:00
abstract::Macrophage migration is mediated by complex cellular signaling processes and cytoskeleton re-arrangement. In particular, recent advances indicate that the innate immunity signaling process plays a key role in the regulation of macrophage migration. In this report, we have provided evidence demonstrating the involvemen...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.12.004
更新日期:2010-03-01 00:00:00
abstract::Aggregation of therapeutic proteins is a key factor in the generation of unwanted immunogenicity, and can result in reduced serum half-life, neutralization of function and adverse health effects. There is currently little information regarding how aggregates interact with B-cell receptors or cognate antibodies at the ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2018.11.020
更新日期:2019-01-01 00:00:00
abstract::Despite the fact that was one of the first systems to be discovered and investigated in the innate immunity, Complement is continuing to receive growing attention by the scientific community. Complement is involved in several diseases such as diabetes, atherosclerosis or Systemic Lupus Erythematous. Successful therape...
journal_title:Molecular immunology
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.molimm.2015.06.003
更新日期:2015-11-01 00:00:00
abstract::High glucose- induced endothelial dysregulation has been recognized as an initiation of vascular complications in Type 2 diabetes mellitus (T2DM). Anagliptin is a novel licensed dipeptidyl peptidase-4 (DPP-4) inhibitor for the treatment of T2DM. The effects of anagliptin in high glucose- induced endothelial dysfunctio...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.01.006
更新日期:2019-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(96)00068-5
更新日期:1996-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2004.09.029
更新日期:2005-05-01 00:00:00
abstract::TRAF6 is a key player at the cross-roads of development and immunity. The analysis of its in vivo molecular function is a great challenge since severe developmental defects and early lethality caused by Traf6 deficiency in knock-out mice interfere with analyses of the immune response. In this study we have used a new ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.08.011
更新日期:2010-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2017.02.012
更新日期:2017-06-01 00:00:00
abstract::We have previously used immunoelectron microscopy (IEM) to generate a three-dimensional map of idiotypic (Id) and isotypic epitopes on the Fab arms of HGAC 39 (Roux et al., 1987, Proc. natn. Acad. Sci. U.S.A. 84, 4984-4988), a mouse IgG3 monoclonal antibody (Mab). In this report, we analyse the geometry of the various...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(94)90167-8
更新日期:1994-06-01 00:00:00
abstract::Properly regulated mitogen-activated protein (MAP) kinase activity is critical for normal thymocyte development. MAP kinases are activated by phosphorylation of tyrosine and threonine, and dual specificity phosphatases (DUSPs) can inactivate MAP kinases by dephosphorylating both tyrosine and threonine. However, a role...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.03.006
更新日期:2006-02-01 00:00:00
abstract::In a recent article published in Molecular Immunology [Furukawa, K., Shimizu, T., Murakami, A., Kono, R., Nakagawa, M., Sagawa, T., Yamato, I., Azuma, T., 2007. Strategy for affinity maturation of an antibody with high evolvability to (4-hydroxy-3-nitrophenyl) acetyl hapten. Mol. Immunol. 44, 2436-2445], the authors m...
journal_title:Molecular immunology
pub_type: 评论,信件
doi:10.1016/j.molimm.2007.07.007
更新日期:2008-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.07.003
更新日期:2008-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.05.010
更新日期:2006-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90152-2
更新日期:1993-08-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2011.06.437
更新日期:2011-09-01 00:00:00
abstract::Human leukocyte antigens (HLA) allele determination is becoming an increasingly important aspect in the field of transplantation as well as in the area of HLA association with a number of diseases. Through Lebanon's history, this country, situated at a crossroads between Europe, Asia and Africa, has been a host for va...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(03)00073-7
更新日期:2003-07-01 00:00:00