Abstract：
:The insect gut participates in initial local immune responses by producing reactive oxygen and nitrogen species as well as anti-microbial peptides to resist pathogenic invasions. Nitric oxide (NO), a signaling and an immune effector molecule synthesized by the enzyme NO synthase (NOS), mediates an early step of the signal transduction pathway. In this study, we evaluated NO levels after Nosema pernyi infection in Antheraea pernyi gut. NOS activity was higher in the microsporidia-infected gut of A. pernyi than in that of control. Three NOS-related genes were cloned, and their spatio-temporal expression patterns were evaluated. ApNOS2 was expressed quickly in the midgut after N. pernyi infection. Sodium nitroprusside, dihydrate (SNP), or Nω-L-nitro-arginine methyl ester, hydrochloride (L-NAME), altered the NO content in A. pernyi midgut. Anti-microbial peptides (AMPs) in the groups exposed to N. pernyi plus SNP and N. pernyi plus L-NAME exhibited higher and lower expression, respectively, relative to the control. These results indicate that microsporidia infection triggers short-term activation of NO and NOS genes in the A. pernyi gut that is downregulated after 24 h. Notably, infection rates can be influenced by a NOS inhibitor. Furthermore, NO can be induced by pathogens. Similarly, NO content in the A. pernyi gut also influences AMPs in humoral immunity and some immune-related genes. Our results suggest that nitric oxide plays a vital role in A. pernyi gut immunity.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Liu W,Wang Y,Leng Z,Wang Q,Duan X,Luo Y,Jiang Y,Qin Ldoi
10.1016/j.molimm.2020.07.018subject
Has Abstractpub_date
2020-10-01 00:00:00pages
65-72eissn
0161-5890issn
1872-9142pii
S0161-5890(20)30433-8journal_volume
126pub_type
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pub_type： 杂志文章
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更新日期：2016-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：1998-01-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1016/j.molimm.2004.07.006
更新日期：2005-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.molimm.2007.01.023
更新日期：2007-05-01 00:00:00
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pub_type： 杂志文章
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journal_title：Molecular immunology
pub_type： 杂志文章
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2016-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2010-11-01 00:00:00
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pub_type： 杂志文章
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90178-m
更新日期：1991-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
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更新日期：2010-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.08.008
更新日期：2016-10-01 00:00:00
abstract：:A number of studies have showed that High mobility group box-1 (HMGB1), which played key role in inflammation activation by triggering the toll like receptor 4 (TLR4) signaling axis in hepatic fibrogenesis, may share similar inflammation stimulating mechanism with LPS. Herein, we introduced a recently established anti...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2015.10.018
更新日期：2016-01-01 00:00:00
