Abstract：
:A number of fragments derived from acid-treated rabbit IgG by digestion with plasmin have been separated by high-resolution gel filtration. Fragments isolated included a dimer and monomer Facb, named F(acb)2 and Facb, respectively and a heterodimer composed of Facb and Fab subunits, named F(acb)(ab). A C gamma 2 fragment was obtained by papain digestion of Facb. A heterodimer composed of Facb and Fab', named F(acb)(ab'), was also prepared by oxidizing a reduced mixture of these fragments. Fragments thus obtained are classified into two groups--those carrying paired C gamma 2 domains, i.e. F(acb)2, and the disulfide-linked dimeric C gamma 2 fragment; and those having a single C gamma 2 domain, i.e. reduced, alkylated Facb and C gamma 2 fragment, F(acb)(ab) and F(acb)(ab'). These fragments exhibited marked differences in their capacity to activate complement in assay systems of hemolysis and complement consumption by immune complexes or aggregates on polystyrene latex. Fragments of the former group could activate complement but with a definitely reduced efficiency (50%) compared to intact IgG, whereas fragments of the latter group were practically inactive. Although it was not determined whether the C1-binding capacity itself is changed by monomerization of the C gamma 2 domain, the results suggested that the intact paired C gamma 2 module is required at least for the activation process of complement.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Utsumi S,Okada M,Udaka K,Amano Tdoi
10.1016/0161-5890(85)90147-6subject
Has Abstractpub_date
1985-07-01 00:00:00pages
811-9issue
7eissn
0161-5890issn
1872-9142journal_volume
22pub_type
杂志文章abstract：:As demonstrated by others, diisopropyl fluorophosphate (DFP) markedly inhibits the O2- generation from guinea-pig polymorphonuclear leukocytes (PMN) stimulated by an antibody complex with ovalbumin (Ag-Ab complex), and also the intracellular uptake of antibody-sensitized erythrocytes by the cells. However, when PMN we...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90113-0
更新日期：1985-09-01 00:00:00
abstract：:Purified human C5 was converted non-enzymically to an activated form as defined by its ability to participate in reactive lysis. This conversion occurred following exposure to systems that generate oxygen radicals, namely addition of H2O2 in the presence of ascorbic acid and iron or the addition of xanthine oxidase, a...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90057-6
更新日期：1989-12-01 00:00:00
abstract：:Cytohesin-1 is a guanine nucleotide exchange factor for ADP ribosylation factor 6 (Arf6) in human blood neutrophils and differentiated PLB-985 neutrophil-like cells. Cytohesin-1 regulates adhesion and the transendothelial migration of monocytes, dendritic cells and T lymphocytes through activation of the β2 integrin L...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.03.018
更新日期：2011-07-01 00:00:00
abstract：:Conserved extended haplotypes (CEHs) are large (>or=1Mb) regions of identical DNA of the major histocompatibility complex (MHC) region of chromosome 6p in unrelated individuals. They are recognized by family studies and constitute nearly half of MHC haplotypes among European Caucasians. We studied 49 Hungarian Caucasi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.03.013
更新日期：2010-06-01 00:00:00
abstract：:Several mutations in the CFH gene have been described in non-Shiga-toxin-associated haemolytic uraemic syndrome (non-Stx-HUS), a rare syndrome characterized by haemolytic anaemia, thrombocytopenia and acute renal failure. Mutations in genes encoding other complement regulatory proteins, membrane cofactor protein (CD46...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.08.004
更新日期：2007-03-01 00:00:00
abstract：:Tehranolide named as new type of a sesquiterpene lactone with an endoperoxide group which purified from Artemisia diffusa and having similar structure to Artemisinin. Tehranolide is hypothesized to possess effects akin to Artemisinin, which is active against cancer cells. In the present research we emphasized on the d...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.01.007
更新日期：2010-04-01 00:00:00
abstract：:The binding specificity of rat alveolar macrophages (AM phi) for sheep erythrocytes (E) coated with gangliosides GM1, GM2, GM3, GD1a, GD1b or GT1b was analyzed in a rosette assay by studying the inhibitory effect of gangliosides, various carbohydrates, IgG, C3b-like C3, and fibronectin in this assay. The uptake of gan...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90163-x
更新日期：1986-11-01 00:00:00
abstract：:Mammalian B-cell specific somatic hypermutation contributes to affinity maturation of the antibody response. This mutator activity is highly focused on rearranged immunoglobulin variable regions, but the underlying mechanism remains to be elucidated. In an effort to gain insights into the mechanism of somatic hypermut...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90081-7
更新日期：1994-02-01 00:00:00
abstract：:The KH type splicing regulatory protein (KSRP) is a nucleic acid binding protein, which negatively regulates the stability and/or translatability of many mRNA species encoding immune-relevant proteins. As KSRP is expressed in immune cells including T and B cells, neutrophils, macrophages and dendritic cells, we wanted...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.05.003
更新日期：2017-07-01 00:00:00
abstract：:Using a combinatorial chemistry approach, we identified a tetrameric tripeptide, denoted Protein A Mimetic (PAM) or TG19318, able to bind to immunoglobulins of different classes and species. The inverso variant, with the tripeptide in the all-D configuration (D-PAM or TG19320), is described as retaining binding proper...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.04.020
更新日期：2008-01-01 00:00:00
abstract：:The O-antigen of the gram negative bacteria Brucella is composed of an homopolymer of 4,6-dideoxy-4-formamido-alpha-D-mannopyranosyl (or perosamine). Several mAb interact specifically with only the O-antigen of certain Brucella species. Although, many studies show that this specific recognition results mainly from the...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2003.11.037
更新日期：2004-03-01 00:00:00
abstract：:The paradigm that B cells mainly play a central role in adaptive immunity may have to be reevaluated because B-1 lineage cells have been found to exhibit innate-like functions, such as phagocytic and bactericidal activities. Therefore, the evolutionary connection of B-1 lineage cells between innate and adaptive immuni...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2015.10.014
更新日期：2016-01-01 00:00:00
abstract：:Mutation of microphthalmia transcription factor (MITF) results in deafness, bone loss, small eyes, and poorly pigmented eyes and skin. The primary cell types affected in MITF-deficient mice are melanocytes, osteoclasts and mast cells. A search for MITF-associated proteins, using a mast cell library that was screened w...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/s0161-5890(02)00059-7
更新日期：2002-09-01 00:00:00
abstract：:C-reactive protein (CRP) binds to chromatin, histones, and small nuclear ribonucleoproteins (snRNPs) through a phosphocholine (PC)-inhibitable, calcium-dependent binding site. snRNPs process pre-mRNA to mature mRNA and are composed of small uridine-rich RNAs (designated U1, U2, U5 and U4/U6) and associated proteins. W...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90141-w
更新日期：1993-06-01 00:00:00
abstract：:Major histocompatibility complex (MHC) has a profound influence on disease resistance or susceptibility, productivity and important economic traits in chicken. Association of the MHC with a wide range of immune responses makes it a valuable predictive factor for the disease pathogenesis and outcome. The tandem repeat ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.06.027
更新日期：2017-10-01 00:00:00
abstract：:Multilamellar lipid vesicles (MLV) composed of egg yolk lecithin (EYL) suppressed the response of bovine peripheral blood lymphocytes (BPBL) to phytohemagglutinin (PHA). EYL contains 18:1 and 18:2 as the major unsaturated phospholipids. Dioleoyllecithin (DOL; cis 9) MLV did not suppress BPBL blastogenesis. Dilinoeylle...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90195-x
更新日期：1982-11-01 00:00:00
abstract：:Cationic antimicrobial peptides play important roles in host defense, linking innate and adaptive immunity. hCAP18, the only human antimicrobial cathelicidin, consists of a conserved N-terminal cathelin-like domain and a C-terminal peptide, LL-37. Expression is regulated during myeloid differentiation, and tightly con...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.04.023
更新日期：2008-01-01 00:00:00
abstract：:Four different proteases were screened for their capability of selectively digesting murine monoclonal IgGl to obtain active F(ab)2. For the screening, a series of five different mouse monoclonal antibodies (IgGl, k) was used, recognizing different tumor-associated antigens and currently used for radioimmunoimaging st...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90088-2
更新日期：1991-01-01 00:00:00
abstract：:Comparative interactions of purified rabbit C1q with 18-2-3, a high affinity (2-3 X 10(10) M-1) anti-fluorescein (anti-F1) murine monoclonal IgM antibody (pentamer) and constitutive monomeric subunits (IgMs) were studied. Using a solid phase radioimmunoassay (SPRIA), based on immobilized polyvalent antigen, it was sho...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90076-4
更新日期：1988-06-01 00:00:00
abstract：:Porphyromonas gingivalis causes periodontal diseases and its lipopolysaccharide (LPS) is considered as a major virulence factor responsible for pathogenesis. Since initial recognition of P. gingivalis LPS (Pg.LPS) in the oral cavity might be crucial for the host response, we identified Pg.LPS-binding proteins (Pg.LPS-...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.06.434
更新日期：2011-09-01 00:00:00
abstract：:SOCS3 is well known to negatively regulate various cytokine-mediated signaling responses, but its direct role in the expression of specific cytokines has not been clearly elucidated. To understand the role of SOCS3 in the expression of IL-4, one of the key Th2 cytokines, RBL-2H3 cells (a rat mast cell line) were engin...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.11.005
更新日期：2011-02-01 00:00:00
abstract：:Recent studies have shown that aberrant activation of the complement system plays an important role in the pathogenesis of preeclampsia. There is evidence to suggest that aberrant activation of the complement system may already be present during the first trimester. Here, we performed a prospective study in which peri...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.03.021
更新日期：2020-04-16 00:00:00
abstract：:Human beta defensin-1 (hBD1) is a component of the immune system which links the innate and adaptive immune responses. We have demonstrated that hBD1 induces rapid cytolysis of prostate cancer cells and that it may also possess tumor suppressive abilities. In addition, there is a high frequency of cancer-specific loss...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.11.004
更新日期：2009-03-01 00:00:00
abstract：:Malignant transformation of hepatocytes is frequently associated with upregulation of HLA-A expression. Currently there is no information available regarding the mechanisms underlying this phenotypic change. We investigated HLA-A expression in 165 paraffin embedded tissues and 21 fresh tissues from liver cancer patien...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.03.001
更新日期：2009-06-01 00:00:00
abstract：:Cathepsin L protease belongs to the papain-like cysteine proteases family, plays indispensable roles in animals' pathological and physiological processes. However, little is known about Cathepsin L in silkworm, Bombyx mori. Herein, a novel Cathepsin L-like (Cat L-like) was cloned and identified from silkworm by the ra...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.12.013
更新日期：2020-12-26 00:00:00
abstract：:Antigens related to the carcinoembryonic antigen (CEA) were isolated from normal human plasma by perchloric acid extraction, gel permeation chromatography and immunoaffinity chromatography using a monoclonal antibody with broad specificity and high affinity. The antigens were separated by SDS-polyacrylamide gel electr...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90046-x
更新日期：1985-11-01 00:00:00
abstract：:The high affinity receptor for IgE (FcvarepsilonRI) is constitutivelly expressed on the surface of mast cells and basophils as a multimeric complex. Upon antigen ligation to FcvarepsilonRI-bound IgE molecules, the receptor complex transduces intracellular signals leading to the release of preformed and newly synthesis...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2010.06.003
更新日期：2010-09-01 00:00:00
abstract：:Structural similarities between two members of the immunoglobulin superfamily were explored by making chimeric immunoglobulin/CD4 antigen molecules. A crossover in the middle of the originally proposed J kappa homology unit of the first domain of the CD4 molecule was used to construct a chimeric molecule having human ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90002-s
更新日期：1993-06-01 00:00:00
abstract：:C-reactive protein (CRP) is an acute-phase protein featuring a homopentameric structure and Ca-binding specificity for phosphocholine (PCh). Expression of CRP is regulated mainly at the transcriptional level with interleukin-6 being the principal inducer of the gene during the acute phase. The crystal structure of CRP...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/s0161-5890(01)00042-6
更新日期：2001-08-01 00:00:00
abstract：:Concanavalin A (Con A)section-binding proteins from mouse spleen leukocytes were characterised by two-dimensional electrophoresis of material precipitated, by Con A plus anti-Con A, from lysates of biosynthetically-labelled cells. Although most cell surface (iodinatable) proteins are known to bind Con A, some of the m...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90030-5
更新日期：1983-04-01 00:00:00
