Abstract：
:Structural similarities between two members of the immunoglobulin superfamily were explored by making chimeric immunoglobulin/CD4 antigen molecules. A crossover in the middle of the originally proposed J kappa homology unit of the first domain of the CD4 molecule was used to construct a chimeric molecule having human and mouse CD4 antigen sequence through the first 108 amino acids and murine J kappa and C kappa sequence thereafter. This molecule was expressed in the presence and absence of an immunoglobulin heavy chain. The resulting proteins were assayed for the expression of CD4 epitopes that should be present based on epitope mapping data. Monomeric, homodimeric, and heavy chain/light chain tetrameric forms of the recombinant protein were secreted and were all detectable with anti-kappa reagents. CD4 antibodies precipitated only the form of the CD4-C kappa light chain protein which appears as a monomer by polyacrylamide gel electrophoresis. Neither the homodimer nor the heavy chain/light chain tetramer were detected with CD4 monoclonal antibodies. An engineered gene having this CD4 antigen first domain joined to the human IgG1 constant region, when coexpressed with a mouse lambda light chain, also failed to express detectable CD4 epitopes. The structural implications of the presence or absence of CD4 epitopes on these proteins is discussed.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Frey T,Estess P,Oi VTdoi
10.1016/0161-5890(93)90002-ssubject
Has Abstractpub_date
1993-06-01 00:00:00pages
797-804issue
9eissn
0161-5890issn
1872-9142journal_volume
30pub_type
杂志文章abstract：:Whether bovine myelin basic protein (BP) and bovine spinal cord protein (SCP) cross-react at the humoral immune level was assessed with a sensitive solid-phase enzyme immunoassay. We found that a hyperimmune anti-SCP serum reacted strongly with SCP and cross-reacted nearly as well with BP. A hyperimmune anti-BP serum ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(84)90159-7
更新日期：1984-10-01 00:00:00
abstract：:We describe several proteins that are components of the rabbit B cell receptor complex. Two proteins (37 kDa and 42 kDa) were found in non-covalent association with IgM expressed on B cells from peripheral blood. These proteins were also immunoprecipitated by anti-B29 (Ig-beta) and anti-mb1 (Ig-alpha) monoclonal antib...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00028-d
更新日期：1995-07-01 00:00:00
abstract：:To date, although a promising anti-inflammatory activity of oligomeric proanthocyanidins (OPCs) has been observed in asthma, the mechanism responsible for these immunomodulatory properties remains obscure. Dendritic cells (DCs) that reside in the airway have been widely perceived as an important contributor to asthma....
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.09.012
更新日期：2017-11-01 00:00:00
abstract：:The functional importance of various cell membrane bound molecules was studied and compared in the NK cytotoxicity and CTL activity. LFA-1 and CD2 participate in both killing functions, while CD3 and CD8/CD4 as well as MHC class I molecules are involved only in CTL activity. Nevertheless CD2- and beta 2-microglobulin ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90164-1
更新日期：1986-11-01 00:00:00
abstract：:The architecture of lymphoid microenvironments depends upon complex interactions between several stromal cell types. We describe in this report the cloning of a cDNA which encodes a novel membrane molecule containing two external Ig-like domains. It is expressed at the junction between endothelial cells including HEV....
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(98)00102-3
更新日期：1998-12-01 00:00:00
abstract：:Spondyloarthritis is the second most common form of chronic inflammatory arthritis and a unique hallmark of the disease is pathologic new bone formation. Several cytokine pathways have been genetically associated with ankylosing spondylitis (AS), the prototypic subtype of SpA, and additional evidence from human and an...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2013.07.016
更新日期：2014-01-01 00:00:00
abstract：:the SC5b-8 complex of human complement is a hydrophilic molecule of mol. wt 800,000-850,000 that is seen in the electron microscope as an elongated, straight or curved structure of 50-55 nm total length and 8-9 nm width. Tryptic attack on the fluid-phase complex exposes lipid-binding surfaces on the molecule. The tryp...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90327-3
更新日期：1982-09-01 00:00:00
abstract：:The ubiquitin conjugating (ubc) E2 enzyme ubc-9 conjugates the ubiquitin-like peptide sentrin/SUMO-1/PIC1 to target proteins which include the Fas antigen. We show that the mouse genome contains four copies of the ubc-9 gene. These include a structural ubc-9 gene consisting of seven exons which encode a protein identi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(98)00094-7
更新日期：1998-11-01 00:00:00
abstract：:Intravenous drug use has become the principal route of hepatitis C virus (HCV) transmission due to the sharing of infected needles. In this study, we analyzed the distribution of HLA-KIR genotypes among 160 Puerto Rican intravenous drug users (IDUs) with HCV infection and 92 HCV-negative Puerto Rican IDUs. We found a ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.05.014
更新日期：2009-08-01 00:00:00
abstract：:The complement system is a vital component of the immune-priveliged human eye that is always active at a low-grade level, preventing harmful intraocular injuries caused by accumulation of turnover products and controlling pathogens to preserve eye homeostasis and vision. The complement system is a double-edged sword t...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2017.05.028
更新日期：2017-09-01 00:00:00
abstract：:In contrast to normal B lymphocytes, splenic B cells from autoimmune NZB mice express on their surface greater amounts of IgM than IgD. We have found that the predominant mu chain species present on these cells is derived from pentameric secretory IgM which can be radioiodinated during transient association with the c...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90026-0
更新日期：1988-04-01 00:00:00
abstract：:Human leukocyte antigens (HLA) allele determination is becoming an increasingly important aspect in the field of transplantation as well as in the area of HLA association with a number of diseases. Through Lebanon's history, this country, situated at a crossroads between Europe, Asia and Africa, has been a host for va...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(03)00073-7
更新日期：2003-07-01 00:00:00
abstract：:Ig production by splenic human B cells that express different surface Ig isotypes were analysed in limiting dilution cultures. Therefore, FACS sorted IgM+, IgG+ and IgA1+ B cells were stimulated with PMA-activated EL4 thymoma cells as helper cells in the presence of IL-2 and IL-4. We found that at least every second B...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90051-x
更新日期：1992-10-01 00:00:00
abstract：:The thermal effects associated with the binding reaction of concanavalin A and immunoglobulins have been measured by calorimetry. IgG solutions do not generate heat on mixing with concanavalin A, confirming the low reactivity of IgG for the lectin molecule. IgM solutions, on the other hand, show a substantial enthalpi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90357-1
更新日期：1982-07-01 00:00:00
abstract：:Epithelial surfaces constitute natural immunobarriers against environmental threats. These barriers are brimming with fluids that bind, transport, cleave or degrade bacterial cells and their endotoxic by-products. Saliva and the airway surface-lining fluid (ASL) comprise the important fluid constituents. Short palate,...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.10.021
更新日期：2006-04-01 00:00:00
abstract：:An expression cDNA library was constructed from the helper T cell hybridoma, A.1.1, which has been shown to produce constitutively proteins involved in the down regulation of the immune response. From this library we identified and characterized a cDNA clone, J6B7, by screening with a polyclonal antibody specific for ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90102-6
更新日期：1990-11-01 00:00:00
abstract：:Keratin is a cytoskeletal protein that constitutes the intermediate filament. Its distribution is restricted to epithelial tissues in mammals, but is wider in fish. An interesting feature of fish keratin is that it is abundant in the cutaneous mucus. However, the biological function of keratin in the mucus has not bee...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.09.012
更新日期：2019-12-01 00:00:00
abstract：:Increasing evidence has demonstrated that the epithelial cells in the lung play crucial roles in regulating certain inflammatory responses by modulating Wnt signaling during microbial infection. However, the anti-microbial functions of Wnt signaling in alveolar epithelial cells remain elusive. In this report, we show ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.02.002
更新日期：2014-06-01 00:00:00
abstract：:Many biological properties of immune complexes (IC) depend upon their size. Quasi-elastic light scattering (QLS) was used to measure a mean equivalent hydrodynamic radius (Rh) and variance of the distribution of model IC composed of bovine serum albumin (BSA) as antigen (Ag) and combinations of two or three well-chara...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90086-7
更新日期：1988-01-01 00:00:00
abstract：:Tunicamycin inhibited the synthesis of glycosylated mu-chains and kappa-chains in the human lymphoblastoid cell line, Daudi. Nonglycosylated IgM could not be detected on the surface of tunicamycin-treated cells by cell surface iodination techniques even under conditions where membrane IgM was re-expressed in control c...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90106-2
更新日期：1983-01-01 00:00:00
abstract：:Interplay between complement factors, regulatory proteins, anaphylatoxins and cytokines could be involved in tendon healing and scar formation. The expression and regulation of complement factors by cytokines or anaphylatoxins are completely unclear in tendon. Hence, the gene expression of the anaphylatoxin receptors ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.09.001
更新日期：2013-04-01 00:00:00
abstract：:The T-cell surface proteins CD4 (L3T4) and CD8 (Lyt2) are first expressed on thymocytes as they undergo maturation in the thymus. Two immature T-lymphoma cell clones SL12.4 and RS4.2 which constitutively express low or undetectable levels of CD4 and CD8 were used to investigate the activation of CD4 and CD8 gene expre...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90087-z
更新日期：1991-01-01 00:00:00
abstract：:The testicular cystic structure and the abrupt morphological changes that the fish testis undergoes during the reproductive cycle (RC) make it an interesting model for studying the regulation of spermatogenesis, in particular the role of matrix metalloproteinases (Mmps). The gilthead seabream is a seasonal breeding te...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.01.031
更新日期：2008-05-01 00:00:00
abstract：:Rabbit antisera specific for horseradish peroxidase inhibit the catalytic activity of the enzyme. All antibodies prepared against the holoenzyme react with the peroxidase apoenzyme. However, only a minority (30-45%) of the total antiperoxidase pool cross react with reduced and alkylated apoenzymes. The antibodies inhi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90366-2
更新日期：1982-05-01 00:00:00
abstract：:Although the etiology of rheumatoid arthritis (RA) remains unknown, it has been widely suggested that RA has a genetic background. In humans, a copy number loss of 22q11.2, a region harboring the VPREB1 gene, has been suggested to be associated with several immunologic disorders, but there has been no study on the cop...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.11.009
更新日期：2011-06-01 00:00:00
abstract：:Mutations within the 5'-non-coding region of the bcl-6 gene can occur in lymphomas that originate from germinal centers (GCs), as well as in normal memory and GC B cells. Mutations in the p53 gene occur in 50% of human cancers. Since both bcl-6 and p53 can be mutated in certain circumstances, we investigated the accum...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(02)00117-7
更新日期：2002-11-01 00:00:00
abstract：:Specific IgE antibodies have been affinity-purified from recombinant grass pollen allergens, and used to identify isoforms of the two major allergens of rye-grass pollen, Lol p I and Lol p V and cross-reactive allergens in other grasses. Lol p I-specific IgE (affinity-purified from the recombinant protein expressed by...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90068-x
更新日期：1994-04-01 00:00:00
abstract：:Recent studies demonstrate that tissue microenvironments may regulate development and functions of immune cells including dendritic cells, monocytes and T cells. However, there is no report about functional regulation of innate NK cells by tissue microenvironment. The liver is an organ abundant in NK cells and suscept...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.01.035
更新日期：2008-05-01 00:00:00
abstract：:Reduced expression of Erythrocyte Complement Receptor 1 (E-CR1) is envisaged to contribute significantly to the pathophysiology of systemic lupus erythematosus (SLE). We determined the levels of CR1 transcript in the neutrophils from 25 untreated patients with active SLE and 25 normal healthy individuals and, studied ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.07.300
更新日期：2007-03-01 00:00:00
abstract：:We have previously used immunoelectron microscopy (IEM) to generate a three-dimensional map of idiotypic (Id) and isotypic epitopes on the Fab arms of HGAC 39 (Roux et al., 1987, Proc. natn. Acad. Sci. U.S.A. 84, 4984-4988), a mouse IgG3 monoclonal antibody (Mab). In this report, we analyse the geometry of the various...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90167-8
更新日期：1994-06-01 00:00:00
