Abstract:
:Using a combinatorial chemistry approach, we identified a tetrameric tripeptide, denoted Protein A Mimetic (PAM) or TG19318, able to bind to immunoglobulins of different classes and species. The inverso variant, with the tripeptide in the all-D configuration (D-PAM or TG19320), is described as retaining binding properties to Ig. This peptide has now been assayed as a binder for E class immunoglobulins, in linear and competitive ELISA experiments, dot-blot and surface plasmon resonance (SPR) analyses. We show that D-PAM binds IgE with high specificity and selectivity, the interaction being sufficient to inhibit anaphylactic release of beta-hexosaminidase from RBL 2H3 cells, with an IC50 value of 10 microg/mL. Intradermal administration of D-PAM suppresses PCA in the rat, with an IC50 of 1.25 microg/kg dose of peptide, while its intraperitoneal injection inhibits mouse PCA with an IC50 of about 7 mg/kg and an efficacy comparable to that of ketotifen. Similarly, D-PAM inhibits ACA in the mouse, with 50% suppression at 10 mg/kg. The results presented here show that the peptide is active on the studied models, with effective doses below toxicity level, hence the molecule is a promising candidate for development of a new class of anti-allergic drugs.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Rossi M,Ruvo M,Marasco D,Colombo M,Cassani G,Verdoliva Adoi
10.1016/j.molimm.2007.04.020subject
Has Abstractpub_date
2008-01-01 00:00:00pages
226-34issue
1eissn
0161-5890issn
1872-9142pii
S0161-5890(07)00168-Xjournal_volume
45pub_type
杂志文章abstract::Multiple organ dysfunction syndrome (MODS) is a major cause of morbidity and mortality in surgical intensive care units. It is characterized by progressive failure of two or more organs remote from the origin of injury. Since MODS results from a severe generalized inflammatory response, both chemokines and complement ...
journal_title:Molecular immunology
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journal_title:Molecular immunology
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2015.03.246
更新日期:2015-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.12.013
更新日期:2008-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.09.034
更新日期:2007-03-01 00:00:00
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journal_title:Molecular immunology
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doi:10.1016/j.molimm.2008.11.002
更新日期:2009-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(91)90134-6
更新日期:1991-06-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2011.08.019
更新日期:2011-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1986-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.01.028
更新日期:2017-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90089-7
更新日期:1987-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2011.11.004
更新日期:2012-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(86)90068-4
更新日期:1986-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(93)90463-l
更新日期:1993-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(94)90175-9
更新日期:1994-06-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(01)00060-8
更新日期:2001-08-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(00)00078-x
更新日期:2000-06-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2014-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2013.01.018
更新日期:2013-07-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2004.09.020
更新日期:2005-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.10.004
更新日期:2016-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2003.10.015
更新日期:2004-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
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更新日期:2018-08-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2018.09.005
更新日期:2018-12-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.07.026
更新日期:2006-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2012.11.005
更新日期:2013-06-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2019.08.002
更新日期:2019-10-01 00:00:00
abstract:BACKGROUND:Bladder cancer (BC) can be successfully treated by manipulating immune responses with intravesical Bacillus Calmette-Guerin instillation or targeting the PD-1/PD-L signaling pathway. In the present study we investigated the prognostic significance of the immune checkpoint inhibitor PD-1 and its ligands PD-L1...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.05.010
更新日期:2020-08-01 00:00:00
abstract::GRX1 (glutaredoxin1), a sulfhydryl disulfide oxidoreductase, is involved in many cellular processes, including anti-oxidation, anti-apoptosis, and regulation of cell differentiation. However, the role of GRX1 in the oxidative stress and apoptosis of osteoarthritis chondrocytes remains unclear, prompting the current st...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.08.006
更新日期:2017-10-01 00:00:00