Abstract：
:Four monoclonal antibodies (MAbs) directed against the P1 blood group antigen were produced by hybridomas obtained from mouse immunized with turtle-dove avomucoid. One of the MAb (154 IX B6) selected as a blood typing reagent agglutinated native P1 and Pk1 red cells with a high titer but was inactive against native P2, Pk2 and p erythrocytes. After papain treatment the reactivity towards P1 and Pk1 erythrocytes was enhanced whereas p erythrocytes remained unreactive. A weak cross-reactivity of the MAb with the Pk antigen was suspected since enzyme-treated Pk2 erythrocytes became significantly agglutinated. Further analysis of the antibody specificity was established by binding studies using neutral glycolipids prepared from P1 and P2 erythrocytes, affinity immunoabsorbents carrying known oligosaccharide structures and hapten inhibition with synthetic oligosaccharides. The MAb bound weakly to the Gal alpha 1-4Gal structure common to P1 and Pk antigens but had a marked preference for the P1 determinant (Gal alpha 1-4 Gal beta 1-4 GlcNAc) and the binding was abolished by prior treatment of oligosaccharide antigens by alpha(not beta)-galactosidase, which supports evidence that a terminal alpha-galactose residue is involved in the blood group P1 and Pk specificities. The MAb has a slightly broader specificity than the human anti-P1 counterpart but can be used safely for routine blood typing.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Bailly P,Chevaleyre J,Sondag D,François-Gérard C,Piquet Y,Vezon G,Cartron JPdoi
10.1016/0161-5890(87)90089-7subject
Has Abstractpub_date
1987-02-01 00:00:00pages
171-6issue
2eissn
0161-5890issn
1872-9142journal_volume
24pub_type
杂志文章abstract：:With the exception of a few strains such as BuB/BnJ, complement activity in most inbred strains of mice is remarkably similar. The BuB/BnJ strain reportedly has high levels of complement activity and elevated serum levels of C3 and other complement proteins. However, we observed that BuB/BnJ mice have serum C3 levels ...
journal_title：Molecular immunology
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journal_title：Molecular immunology
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journal_title：Molecular immunology
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journal_title：Molecular immunology
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journal_title：Molecular immunology
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更新日期：2002-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：1999-01-01 00:00:00
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更新日期：2007-02-01 00:00:00
