Cellular uptake followed by class I MHC presentation of some exogenous peptides contributes to T cell stimulatory capacity.


:The T cell stimulatory activity of peptides is known to be associated with the cell surface stability and lifetime of the peptide-MHC (pepMHC) complex. In this report, soluble high-affinity T cell receptors (TCRs) that are specific for pepMHC complexes recognized by the mouse CD8+ clone 2C were used to monitor the cell surface lifetimes of synthetic agonist peptides. In the 2C system, L(d)-binding peptide p2Ca (LSPFPFDL) has up to 10,000-fold lower activity than peptide QL9 (QLSPFPFDL) even though the 2C TCR binds to p2Ca-L(d) and QL9-L(d) complexes with similar affinities. Unexpectedly, p2Ca-L(d) complexes were found to have a longer cell surface lifetime than QL9-L(d) complexes. However, the strong agonist activity of QL9 correlated with its ability to participate in efficient intracellular delivery followed by cell surface expression of the peptide, resulting in high and persistent surface levels of QL9-L(d). The ability of target cells to take up and present QL9 was observed with TAP-deficient cells and TAP-positive cells, including dendritic cells. The process was brefeldin A-sensitive, indicating a requirement for transport of the pepMHC through the ER and/or golgi. Thus, strong T cell stimulatory activity of some pepMHC complexes can be accomplished not only through long cell surface lifetimes of the ligand, but through a mechanism that leads to delayed presentation of the exogenous antigen after intracellular uptake.


Mol Immunol


Molecular immunology


Brophy SE,Jones LL,Holler PD,Kranz DM




Has Abstract


2007-03-01 00:00:00














  • Mechanisms of killing by anti-CD20 monoclonal antibodies.

    abstract::CD20 is a cell-surface marker expressed on mature B cells and most malignant B cells, but not stem or plasma cells. It is an ideal target for monoclonal antibodies (mAb), such as rituximab and ofatumumab, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the p...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Glennie MJ,French RR,Cragg MS,Taylor RP

    更新日期:2007-09-01 00:00:00

  • Effects of IL-4 and Fc gamma receptor II engagement on Egr-1 expression during stimulation of B lymphocytes by membrane immunoglobulin crosslinking.

    abstract::Egr-1 is an immediate early gene that is rapidly upregulated in response to mitogenic signals induced by antigen receptor crosslinking on murine B lymphocytes. It has been shown that levels of Egr-1 expression are closely correlated with B cell proliferation in several models of B cell activation and tolerance. We com...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Klaus SJ,Phillips NE,Parker DC

    更新日期:1993-11-01 00:00:00

  • Fine specificity and sequence of antibodies directed against the ectodomain of matrix protein 2 of influenza A virus.

    abstract::The ectodomain of matrix protein 2 (M2e) has remained remarkably conserved amongst human influenza A viruses and is a target for Abs with protective activity. For these reasons, M2e is being investigated for its potential as a broadly protective influenza A virus vaccine. Here, we report on the fine specificity and se...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Zhang M,Zharikova D,Mozdzanowska K,Otvos L,Gerhard W

    更新日期:2006-07-01 00:00:00

  • Relative importance of different human aPL derived heavy and light chains in the binding of aPL to cardiolipin.

    abstract:INTRODUCTION:Previous studies have suggested the importance of somatic mutations and arginine, asparagine and lysine residues in the complementarity determining regions (CDRs) of antiphospholipid antibodies (aPL) implicated in the pathogenesis of the antiphospholipid antibody syndrome. The relative contributions of the...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Giles IP,Haley J,Nagl S,Latchman DS,Chen PP,Chukwuocha RU,Isenberg DA,Rahman A

    更新日期:2003-09-01 00:00:00

  • Functional analysis of naturally occurring amino acid substitutions in human IFN-gammaR1.

    abstract::IFN-gamma plays an essential role in the IL-12/IL-23/IFN-gamma pathway that is required for the defense against intracellular pathogens. In the IFN-gammaR1 several amino acid substitutions have been reported that abrogate IFN-gamma signaling. These substitutions can lead to a null phenotype and enhanced susceptibility...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: van de Wetering D,de Paus RA,van Dissel JT,van de Vosse E

    更新日期:2010-02-01 00:00:00

  • The human gamma/delta + and alpha/beta + T cells: a branched pathway of differentiation.

    abstract::In human peripheral blood, most of the CD3+ cells express the alpha/beta T cell receptor. A smaller fraction of CD3+ T cells express the gamma/delta T cell receptor (from 1 to 10% depending the individuals, with an average of 3-5%). Interestingly, although the alpha/beta + T cells never express the gamma chain at the ...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Alexandre D,Lefranc MP

    更新日期:1992-04-01 00:00:00

  • Allogeneic and syngeneic class I MHC complexes drive the association of CD8 and TCR on 2C T cells.

    abstract::In most cases, cytotoxic T cell activation is dependent on the interaction of the T cell receptor (TCR) and CD8 with MHC class I molecules. In the CD8(+) T cell system based on the mouse cytotoxic T cell clone 2C, recognition of the allogeneic MHC L(d) exhibits a less significant role for CD8 than recognition of the s...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Lee PU,Kranz DM

    更新日期:2003-01-01 00:00:00

  • THP-1 and human peripheral blood mononuclear cell-derived macrophages differ in their capacity to polarize in vitro.

    abstract::Macrophages (Mφ) undergo activation to pro-inflammatory (M1) or anti-inflammatory (M2) phenotypes in response to pathophysiologic stimuli and dysregulation of the M1-M2 balance is often associated with diseases. Therefore, studying mechanisms of macrophage polarization may reveal new drug targets. Human Mφ polarizatio...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Shiratori H,Feinweber C,Luckhardt S,Linke B,Resch E,Geisslinger G,Weigert A,Parnham MJ

    更新日期:2017-08-01 00:00:00

  • Inhibition of PIM1 kinase attenuates bleomycin-induced pulmonary fibrosis in mice by modulating the ZEB1/E-cadherin pathway in alveolar epithelial cells.

    abstract::PIM1 is serine/threonine protein kinase that is involved in numerous biological processes. Pulmonary fibrosis (PF) is a chronic pathological result of the dysfunctional repair of lung injury without effective therapeutic treatments. In the current study, we investigated whether PIM1 inhibition would improve bleomycin ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Zhang X,Zou Y,Liu Y,Cao Y,Zhu J,Zhang J,Chen X,Zhang R,Li J

    更新日期:2020-09-01 00:00:00

  • The pivotal role of the mentor in triggering the research on Complement system.

    abstract::Despite the fact that was one of the first systems to be discovered and investigated in the innate immunity, Complement is continuing to receive growing attention by the scientific community. Complement is involved in several diseases such as diabetes, atherosclerosis or Systemic Lupus Erythematous. Successful therape...

    journal_title:Molecular immunology

    pub_type: 历史文章,杂志文章,评审


    authors: Castellano G

    更新日期:2015-11-01 00:00:00

  • Exogenous interleukin-2 does not reverse the immunoinhibitory effects of 1,25-dihydroxyvitamin D3 on human peripheral blood lymphocyte immunoglobulin production.

    abstract::1,25-Dihydroxyvitamin D3 (1,25-D3) is known to have potent inhibitory effects on human peripheral blood mononuclear cell (PBMC) functions. Previous experiments suggest that addition of interleukin-2 (IL-2) to cell cultures can reverse the antiproliferative action of 1,25-D3. Previous studies have also shown that the C...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Jordan SC,Lemire JM,Sakai RS,Toyoda M,Adams JS

    更新日期:1990-01-01 00:00:00

  • Definition of the peptide binding motif within DRB1*1401 restricted epitopes by peptide competition and structural modeling.

    abstract::This study identified the peptide-binding motif of HLA-DRA/DRB1*1401 (DR1401). First, peptides containing DR1401 restricted epitopes were identified using tetramer-guided epitope mapping. Among these, an influenza B peptide was selected for the motif study. After confirming the binding register for this peptide using ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: James EA,Moustakas AK,Berger D,Huston L,Papadopoulos GK,Kwok WW

    更新日期:2008-05-01 00:00:00

  • Structural and functional comparison of MIF ortholog from Plasmodium yoelii with MIF from its rodent host.

    abstract::Host-derived macrophage migration inhibitory factor (MIF) has been implicated in the pathogenesis of malaria infection, especially in malarial anemia. Although two Plasmodium parasite-derived MIF orthologs, Plasmodium falciparum MIF and P. berghei MIF were identified recently, the crystal structure and the precise rol...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Shao D,Zhong X,Zhou YF,Han Z,Lin Y,Wang Z,Bu L,Zhang L,Su XD,Wang H

    更新日期:2010-01-01 00:00:00

  • A Wnt/β-catenin negative feedback loop represses TLR-triggered inflammatory responses in alveolar epithelial cells.

    abstract::Increasing evidence has demonstrated that the epithelial cells in the lung play crucial roles in regulating certain inflammatory responses by modulating Wnt signaling during microbial infection. However, the anti-microbial functions of Wnt signaling in alveolar epithelial cells remain elusive. In this report, we show ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Li Y,Shi J,Yang J,Ma Y,Cheng L,Zeng J,Hao X,Ma C,Wang Y,Liu X

    更新日期:2014-06-01 00:00:00

  • Regulation and functions of NLRP3 inflammasome during influenza virus infection.

    abstract::The NLRP3 inflammasome constitutes a major antiviral host defense mechanism during influenza virus infection. Inflammasome assembly in virus-infected cells facilitates autocatalytic processing of pro-caspase-1 and subsequent cleavage and secretion of proinflammatory cytokines IL-1β and IL-18. The NLRP3 inflammasome is...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Kuriakose T,Kanneganti TD

    更新日期:2017-06-01 00:00:00

  • Acute arsenic exposure induces inflammatory responses and CD4+ T cell subpopulations differentiation in spleen and thymus with the involvement of MAPK, NF-kB, and Nrf2.

    abstract::Increasing lines of evidence indicate that arsenic may be associated with immune related problems, but its detailed effects on immune organs are poorly understood. The objective of this study was to explore inflammatory responses and T cell differentiation of arsenic exposure in spleen and thymus. Female C57BL/6 mice ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Duan X,Gao S,Li J,Wu L,Zhang Y,Li W,Zhao L,Chen J,Yang S,Sun G,Li B

    更新日期:2017-01-01 00:00:00

  • Molecular cloning and characterization of CD9 cDNA from cartilaginous fish, red stingray, Dasyatis akajei.

    abstract::CD9 is a glycoprotein of the transmembrane 4 superfamily (TM4SF) and is involved in various cellular processes. In this study, we describe the isolation of the full-length cDNA encoding for CD9 molecule (daCD9) of red stingray, Dasyatis akajei. This 1252 bp cDNA was isolated from leukocyte cDNA library and contains 68...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Zhu J,Yan K,Lu L,Peng C,Zhou C,Chen S,Xie X,Dong M,Xu A

    更新日期:2006-04-01 00:00:00

  • Cell surface glycoproteins of rabbit lymphocytes: characterization with monoclonal antibodies.

    abstract::The structural characteristics of antigens recognized by a panel of monoclonal antibodies prepared against a rabbit T-lymphocyte cell line have been investigated. Those antigens which could be isolated using immunoadsorbents prepared from the monoclonal antibodies had mol. wts of 42,000, 90,000 and 120,000. The 42,000...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Wilkinson JM,Wetterskog DL,Sogn JA,Kindt TJ

    更新日期:1984-01-01 00:00:00

  • Antigenic sites on the arginine-rich carboxyl-terminal domain of the capsid protein of hepatitis B virus distinct from hepatitis B core or e antigen.

    abstract::The capsid protein of hepatitis B virus (P19) is made of 183 amino acids and carries the antigenic sites of hepatitis B core antigen (HBcAg) and hepatitis B e antigen (HBeAg) on the amino-terminal domain. The carboxyl-terminal domain of P19 (amino acids 150-183) is arginine-rich (47%) and faces the interior of the nuc...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Machida A,Ohnuma H,Takai E,Tsuda F,Tanaka T,Naito M,Munekata E,Miyakawa Y,Mayumi M

    更新日期:1989-04-01 00:00:00

  • Type III interferon gene expression in response to influenza virus infection in chicken and duck embryonic fibroblasts.

    abstract::Type III interferons (IFN-λs) comprise a group of newly identified antiviral cytokines that are functionally similar to type I IFNs and elicit first-line antiviral responses. Recently, type III IFNs were identified in several species; however, little information is available about type III IFNs in ducks. We compared t...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Zhang Z,Zou T,Hu X,Jin H

    更新日期:2015-12-01 00:00:00

  • Junctional diversity prevents negative selection of an antigen-specific T cell repertoire.

    abstract::Endogenous mouse mammary tumor proviruses (MMTV; Mtv loci) deletes Vbeta6 expressing T cells in the thymus of Mtv-7(+) DBA/2 (H2(d)) mice through negative selection. We found that in Mtv-7(-) BALB/c (H2(d)) mice, Vbeta6 is a dominant V gene used in T cell responses to an 18 amino acid long peptide antigen: EYKEYAEYAEY...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Kilgannon P,Novak Z,Fotedar A,Singh B

    更新日期:2010-02-01 00:00:00

  • Absent in Melanoma 2 (AIM2) limits pro-inflammatory cytokine transcription in cardiomyocytes by inhibiting STAT1 phosphorylation.

    abstract::Interferon (IFN)-γ is highly upregulated during heart inflammation and enhances the production of pro-inflammatory cytokines. Absent in Melanoma 2 (AIM2) is an IFN-inducible protein implicated as a component of the inflammasome. Here we seek to determine the role of AIM2 during inflammation in cardiac cells. We found ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Furrer A,Hottiger MO,Valaperti A

    更新日期:2016-06-01 00:00:00

  • Single amino acid substitutions in V(H) CDR2 are sufficient to generate or enhance the specificity of two forms of an anti-arsonate antibody variable region for DNA.

    abstract::We previously showed that a variety of amino acid substitutions at positions 58 and 59 in the V(H) CDR2 of an anti-arsonate (Ars) antibody Fab simultaneously resulted in increased or unaltered affinity for Ars and substantially enhanced affinity for DNA. To test the generality of these observations, we generated and c...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Hande S,Manser T

    更新日期:1997-12-01 00:00:00

  • Receptors for complement C3 on T-lymphocytes: relics of evolution or functional molecules?

    abstract::Despite the fact that receptors for complement on T-cells have been described many years ago the function remains unclear as is the role of complement in the T-cell response. In this review we will evaluate how the accumulated wisdom concur with the current concepts of the adaptive T-cell response. ...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Wagner C,Hänsch GM

    更新日期:2006-01-01 00:00:00

  • 1,25(OH)2D3 induces regulatory T cell differentiation by influencing the VDR/PLC-γ1/TGF-β1/pathway.

    abstract::Vitamin D has been recommended as an immune modulator in recent years, in addition to regulating calcium-phosphorous-bone metabolism. Clinical studies on organ transplantation found that vitamin D sufficiency patients were less likely to develop acute cellular rejection within one year after transplantation compared t...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Zhou Q,Qin S,Zhang J,Zhon L,Pen Z,Xing T

    更新日期:2017-11-01 00:00:00

  • Characterization of an IgY-like low molecular weight immunoglobulin class in the Mexican axolotl.

    abstract::The general thinking about the phylogenic distribution of vertebrate Ig classes is that fish and urodele amphibians are only able to synthesize polymeric IgM-like molecules and that the emergence of a new class of LMW Ig occurs for the first time in anouran species. Following immunization of the Mexican axolotl (Ambys...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Fellah JS,Charlemagne J

    更新日期:1988-12-01 00:00:00

  • Clearance of Propionibacterium acnes by kupffer cells is regulated by osteopontin through modulating the expression of p47phox.

    abstract::Osteopontin (OPN) is a cytokine with multiple functions, including the regulation of innate immune response. However, the detailed function and mechanism of OPN in host defense against invaded microorganisms remain unclear. In this report, we revealed that OPN could affect the clearance of Propionibacterium acnes in k...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Yang H,Guo H,Fan K,Zhang B,Zhao L,Hou S,Qian W,Zhang D,Wang H,Dai J,Guo Y

    更新日期:2011-09-01 00:00:00

  • The RNA binding protein tristetraprolin influences the activation state of murine dendritic cells.

    abstract::Dendritic cells (DCs) serve to maintain peripheral tolerance under steady state conditions. Upon triggering by activation signals they initiate strong immune responses. The activation of DCs is accompanied by a rapid upregulation of proinflammatory cytokines, which were shown in other cell types to be regulated by mec...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Bros M,Wiechmann N,Besche V,Art J,Pautz A,Grabbe S,Kleinert H,Reske-Kunz AB

    更新日期:2010-02-01 00:00:00

  • Prevention and control of reciprocal T-B cell diversification: implications for lupus-like autoimmunity.

    abstract::Autoimmunity is fundamentally a continuously evolving process. The autoimmune responses shift, drift and diversify with time not only to other epitopes in the original antigen but also to other related and sometimes to unrelated antigens. We have described a form of immune diversification--reciprocal T-B epitope sprea...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Singh RR

    更新日期:2004-02-01 00:00:00

  • Complement regulates inhalation tolerance at the dendritic cell/T cell interface.

    abstract::Pulmonary exposure to innocuous aeroallergens is a common event leading to inhalation tolerance. Distinct subsets of pulmonary dendritic cells (DC) and regulatory T cells (T(Reg)) play critical roles in mediating and maintaining such tolerance. In asthmatics, the same aeroallergens drive a maladaptive, Th2-biased immu...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Köhl J,Wills-Karp M

    更新日期:2007-01-01 00:00:00