Abstract：
:The structural characteristics of antigens recognized by a panel of monoclonal antibodies prepared against a rabbit T-lymphocyte cell line have been investigated. Those antigens which could be isolated using immunoadsorbents prepared from the monoclonal antibodies had mol. wts of 42,000, 90,000 and 120,000. The 42,000 mol. wt molecule is similar or identical to a rabbit class I major histocompatibility complex antigen and its characterization has been reported elsewhere. Three different 90,000 mol. wt proteins can be distinguished by their reactivity with lectins and by sequential immunoprecipitation. The 120,000 mol. wt protein is a very abundant surface glycoprotein that appears to be a specific marker for T-cells in the rabbit. It is the immunodominant antigen in a lentil lectin bound glycoprotein pool. Over half of the antibodies were directed against this antigen. All antigens detected by the panel of monoclonal antibodies have been detected on normal lymphoid cells.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Wilkinson JM,Wetterskog DL,Sogn JA,Kindt TJdoi
10.1016/0161-5890(84)90094-4subject
Has Abstractpub_date
1984-01-01 00:00:00pages
95-103issue
1eissn
0161-5890issn
1872-9142journal_volume
21pub_type
杂志文章abstract：:We have examined the properties of soluble guanylate cyclase activity in the human neutrophil. The enzyme showed complex regulation by metal ions. A 10-fold higher activity was observed in the presence of Mn2+ than Mg2+, while Ca2+ caused an increase in activity only in the presence of Mg2+ ion. Sodium nitroprusside (...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90138-5
更新日期：1985-07-01 00:00:00
abstract：:Cutaneous leishmaniasis (CL) mainly caused by Leishmania braziliensis is a chronic inflammatory disease widely spread in Brazil. Genetic variant strains of this parasite have been associated with atypical clinical manifestations of CL in an endemic area in Brazil. Furthermore, these strains have presented distinct bio...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2018.12.013
更新日期：2019-02-01 00:00:00
abstract：:We have sequenced the TCRs from Ld-specific alloreactive T cell hybridomas, whose reactivities we have found to be quite representative of those of a primary dm2 anti-BALB/cJ mixed lymphocyte reaction. We find V beta 6, V beta 7, V beta 8 and V beta 10 gene segments. V alpha usage is diverse, although closely related ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(96)00034-x
更新日期：1996-06-01 00:00:00
abstract：:Activation-induced cell death (AICD) in T lymphocytes depends on the expression of Fas-ligand, which triggers the apoptotic process after binding to its receptor Fas. This leads to the activation of cysteine proteases of the caspase family and especially of caspase-3, a critical effector protein during AICD. We have p...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.12.011
更新日期：2005-07-01 00:00:00
abstract：:The unique species specificity of the bacterial cytolysin intermedilysin is explained by its requirement for the human complement regulator CD59 as the primary receptor. Binding studies using individual domains of intermedilysin mapped the CD59-binding site to domain 4 and swap mutants between human and rabbit (non-in...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.01.003
更新日期：2009-04-01 00:00:00
abstract：:The complement system plays a central role in a number of human inflammatory diseases, and there is a significant need for development of complement-directed therapies. The discovery of an arsenal of anti-complement proteins secreted by the pathogen Staphylococcus aureus brought with it the potential for harnessing th...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2015.05.012
更新日期：2015-10-01 00:00:00
abstract：:The primary immune response against Staphylococcus aureus is mediated by neutrophils. In response to S. aureus and its proteins, neutrophil shows two different kinds of NETosis, viz. suicidal and vesicular NETosis. Glucose is the major energy source of neutrophils for performing NETosis. However, NETosis was found alt...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.06.015
更新日期：2020-08-01 00:00:00
abstract：:The human monocyte-like cell line, U-937, was shown to synthesize and secrete C3 by hemolytic assays, radioimmunoassays and metabolic labeling experiments. The daily synthesis of antigenic C3 by unstimulated U-937 cells was low (about 3 ng/10(6) cells/24 hr) over a 3 day period. Induction of the cells to differentiate...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90079-4
更新日期：1987-10-01 00:00:00
abstract：:The T-cell surface proteins CD4 (L3T4) and CD8 (Lyt2) are first expressed on thymocytes as they undergo maturation in the thymus. Two immature T-lymphoma cell clones SL12.4 and RS4.2 which constitutively express low or undetectable levels of CD4 and CD8 were used to investigate the activation of CD4 and CD8 gene expre...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90087-z
更新日期：1991-01-01 00:00:00
abstract：:The T-dependent BALB/c murine immune response to fluorescein (F1) is characterized by structural heterogeneity at the protein level exemplified in part by a significantly wide range of affinities (Ka), and apparent lack of dominant idiotypes. In order to generate an idiotype family, xenogenic anti-idiotype (anti-ID) a...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90072-0
更新日期：1985-08-01 00:00:00
abstract：:The complement system is very tightly regulated by fluid-phase and membrane-bound factors that prevent injury to self-tissues. The study of genetically engineered animals with targeted deletion or gain of function mutations has highlighted the important role that many of the complement inhibitors play in vivo. The adv...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/s0161-5890(03)00110-x
更新日期：2003-09-01 00:00:00
abstract：:The analysis of the antigenic structure of human interferon (IFN)-alpha 1 with a panel of monoclonal antibodies revealed four immunodominant regions. Three of them, recognized by 12 of 14 antibodies were mapped into the aminoterminal portion of IFN-alpha 1 around residues 31-38, 43-53 and 63-85. The region 31-85 prove...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90124-g
更新日期：1992-07-01 00:00:00
abstract：:The lymphocyte antigen CD6 has been extensively studied in humans, but has not been reported in mouse. Here we describe the isolation and characterization of a cDNA clone encoding the murine homologue of the human lymphocyte antigen CD6. Comparison of the predicted amino acid sequence of human and murine CD6 shows tha...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)00166-x
更新日期：1995-01-01 00:00:00
abstract：:We have developed two distinct solid-phase immunoassays for the detection of antigen binding activity by products of antigen binding T cell hybridomas in the absence of MHC. Two suppressor T cell hybridomas studied (34s-18 and 34s-704) are specific for keyhole limpet hemocyanin, a protein antigen, and the other suppre...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90043-6
更新日期：1989-07-01 00:00:00
abstract：:Mammary epithelia and the epidermis share a common embryologic origin. Like resident epidermal T cells (ETC), a significant number of murine mammary gland T cells (MTC) express Vgamma5-T-cell receptors (TCR). MTC were assessed to see if they express the same invariant Vgamma5/Vdelta1-TCRs as those of ETC. MTC-derived ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.05.010
更新日期：2006-03-01 00:00:00
abstract：:Light chains of 426 randomly selected myelomas from BALB/c and NZB mice were characterized by isoelectric focusing (IF) and electrophoresis at pH 3. Approximately 50% of the light chains in each strain were found to have unique electrophoretic properties. The remaining 50% of light chains fell into groups of two or mo...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(84)90040-3
更新日期：1984-05-01 00:00:00
abstract：:Forty different PCR clones encoding a llama variable heavy chain domain were analysed. The majority of these clones are derived from heavy-chain antibody cDNA in which the entire CH1 exon is absent. It appears from the amino acid within the VHH framework 1 and 3 that all the llama clones belong to the VH III family. H...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(97)00146-6
更新日期：1997-11-01 00:00:00
abstract：:As an initial step towards the molecular analysis of the murine V kappa locus, a cosmid library from BALB/cJ mouse liver DNA was screened with probes representing 10 V kappa families. Of eight cosmids that were isolated from the initial screen, five contained a single restriction fragment that hybridized to the probes...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90015-p
更新日期：1992-03-01 00:00:00
abstract：:Chemokines are key regulators of migration and consequent activation of migrating leukocytes. CC chemokines constitute the largest chemokine group with 24-28 members in mammalian species, and even more in teleost fish, with up to 81 members in zebrafish Danio rerio. Further studies concerning fish chemokine genes will...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.12.014
更新日期：2010-03-01 00:00:00
abstract：:The paper evaluates the role of the concept of degeneracy in current immunology. The historical background of the notion is considered, as well as that of related concepts like 'cross-reactivity' and 'promiscuity'. A distinction is made between degeneracy, as a property of a system and promiscuity, as a property of an...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2003.11.021
更新日期：2004-02-01 00:00:00
abstract：:The molecular structure of protein and epitope mapping strategies are required to engineer the epitopes of a protein. In the present study, IgE binding regions of osmotin were identified and mutated to obtain hypoallergenic variant. The three dimensional (3-D) model of osmotin obtained by homology modeling comprised o...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.10.019
更新日期：2015-03-01 00:00:00
abstract：:Activation-induced cytidine deaminase (AID) is critical for immunoglobulin (Ig) diversification in B cells. The majority of evidence supports the model that AID modifies Ig genes at the DNA level by deaminating cytosines into uracils. The mutagenic activity is largely restricted to Ig genes to avoid genomic damage in ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.07.027
更新日期：2009-10-01 00:00:00
abstract：:Syngeneic polyclonal antibodies were elicited to an affinity labeled high affinity (2-3 X 10(10) M-1) anti-fluorescein murine IgG2a monoclonal antibody. Hyperimmune ascites fluid was tested for reactivity with homologous liganded, affinity labeled and non-liganded Fab fragments derived from the high affinity antibody....
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90111-3
更新日期：1988-08-01 00:00:00
abstract：:Antibodies (Ab) are bifunctional molecules with two domains, a constant region (C) that confers effector properties and a variable (V) region responsible of antigen (Ag) binding. Historically the C and V regions were considered to be functionally independent, with Ag specificity being solely determined by the V region...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.02.018
更新日期：2010-04-01 00:00:00
abstract：:The cell-surface receptor ST2L triggers cytokine release by immune cells upon exposure to its ligand IL-33. To study the effect of ST2L-dependent signaling in different cell types, we generated antagonist antibodies that bind different receptor domains. We sought to characterize their activities in vitro using both tr...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.05.019
更新日期：2016-07-01 00:00:00
abstract：:Mammalian B-cell specific somatic hypermutation contributes to affinity maturation of the antibody response. This mutator activity is highly focused on rearranged immunoglobulin variable regions, but the underlying mechanism remains to be elucidated. In an effort to gain insights into the mechanism of somatic hypermut...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90081-7
更新日期：1994-02-01 00:00:00
abstract：:Hepatitis B virus (HBV) infection afflicts over 350 million people worldwide and is a leading cause of hepatitis, cirrhosis and hepatocellular carcinoma. HBV replicates noncytopathically in hepatocytes, and most of the hepatic injury is caused by the immune response to the virus. While most studies focused on the adap...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.09.022
更新日期：2009-12-01 00:00:00
abstract：:The functional importance of various cell membrane bound molecules was studied and compared in the NK cytotoxicity and CTL activity. LFA-1 and CD2 participate in both killing functions, while CD3 and CD8/CD4 as well as MHC class I molecules are involved only in CTL activity. Nevertheless CD2- and beta 2-microglobulin ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90164-1
更新日期：1986-11-01 00:00:00
abstract：:Surfactant protein D (SP-D) interacts with various different microorganisms and plays an important role in pulmonary innate immunity. SP-D expression has also been detected in extrapulmonary tissues, including the gastro-intestinal tract. However, its function in the intestine is unknown and may differ considerably fr...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.03.013
更新日期：2007-07-01 00:00:00
abstract：:An important function of Fcγ receptors is the removal of IgG-containing immune complexes from the circulation. The activating receptor FcγRIIA and inhibitory receptor FcγRIIB2 are both expressed on human myeloid cells, and are both capable of mediating endocytosis of immune complexes. We studied endocytosis of these t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.09.003
更新日期：2011-10-01 00:00:00
