Abstract：
:The T-cell surface proteins CD4 (L3T4) and CD8 (Lyt2) are first expressed on thymocytes as they undergo maturation in the thymus. Two immature T-lymphoma cell clones SL12.4 and RS4.2 which constitutively express low or undetectable levels of CD4 and CD8 were used to investigate the activation of CD4 and CD8 gene expression. The protein synthesis inhibitors cycloheximide (CHX) and pactamycin rapidly and reversibly increased CD4 and CD8 mRNA in the cloned cell lines, suggesting that a labile inhibitor protein(s) may regulate the expression of these transcripts. Cell surface CD4 and CD8 proteins were transiently detectable following a pulse of CHX. Thymic epithelial cell lines also induced CD4 and CD8 mRNA and cell surface protein, as well as TCR-alpha mRNA when co-cultivated with SL12.4 T lymphoma cells. The increase in CD4 and CD8 was modest, but stable for at least 22 cell generations after the thymic epithelial inducer cells were removed. Epithelial cells of non-thymic origin did not cause induction of these T-cell differentiation markers in SL12.4 T-lymphoma cells. Since the induction elicited by thymic epithelial cells and protein synthesis inhibitors differed dramatically in kinetics and reversibility, it is likely that these inducers act, at least in part, via different mechanisms. This lymphoma model system may be useful for analysis of molecular events which occur in immature thymocytes undergoing differentiation.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Wilkinson MF,Fong AM,Huynh H,Hays EF,MacLeod CLdoi
10.1016/0161-5890(91)90087-zsubject
Has Abstractpub_date
1991-01-01 00:00:00pages
57-68issue
1-2eissn
0161-5890issn
1872-9142journal_volume
28pub_type
杂志文章abstract：:The pentacyclic triterpene α,β-amyrin has been previously reported as an effective compound in the treatment of several inflammatory conditions. Recent evidence indicates that α,β-amyrin displayed its effects through interaction with the cannabinoid pathway. We assessed the anti-inflammatory effects of the α,β-amyrin ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2013.01.018
更新日期：2013-07-01 00:00:00
abstract：:The complement system is important for protection from invading pathogens, removal of waste products and guidance of the immune response. Furthermore, complement can be also targeted to cancer cells. However, membrane-bound inhibitors over-expressed by certain types of tumor cells restrict the cytotoxic activity of co...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.04.025
更新日期：2008-01-01 00:00:00
abstract：:Calcium signals are crucial for diverse cellular functions including adhesion, differentiation, proliferation, effector functions and gene expression. After engagement of the B cell receptor, the intracellular calcium ion (Ca(2+)) concentration is increased promoting the activation of various signaling cascades. While...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2013.10.006
更新日期：2014-12-01 00:00:00
abstract：:We have recently developed an in vitro culture model enabling the large-scale expansion of switched-memory B lymphocytes, producing a polyclonal human IgG repertoire. Given the importance of glycosylation for the functions of immunoglobulins, we analyzed the N-glycosylation profiles of the immunoglobulin G (IgG) in th...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2013.10.005
更新日期：2014-02-01 00:00:00
abstract：BACKGROUND:Atopic dermatitis (AD) or atopic eczema is a skin disease characterized with itching, increased serum level of immunoglobulin E, and peripheral eosinophilia. Thymoquinone (TQ) is an important ingredient of Nigella sativa seeds having antioxidant and anti-inflammatory effects. OBJECTIVE:Present study investi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2018.07.013
更新日期：2018-09-01 00:00:00
abstract：:Signal transduction by the B cell receptor (BCR) is an absolute requirement for the selection and development of B lymphocytes at multiple checkpoints. Binding to antigen via the BCR is complemented by a co-stimulus delivered through accessory and co-stimulatory cell surface molecules that regulate the signalling thre...
journal_title：Molecular immunology
pub_type：
doi：10.1016/s0161-5890(02)00203-1
更新日期：2003-01-01 00:00:00
abstract：:A number of fragments derived from acid-treated rabbit IgG by digestion with plasmin have been separated by high-resolution gel filtration. Fragments isolated included a dimer and monomer Facb, named F(acb)2 and Facb, respectively and a heterodimer composed of Facb and Fab subunits, named F(acb)(ab). A C gamma 2 fragm...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90147-6
更新日期：1985-07-01 00:00:00
abstract：:Humanization of monoclonal antibodies by complementary determinant region (CDR)-grafting has become a standard procedure to improve the clinical usage of animal antibodies. However, antibody humanization may result in loss of activity that has been attributed to structural constraints in the framework structure. In th...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(03)00022-1
更新日期：2003-05-01 00:00:00
abstract：:Peptide binding to major histocompatibility complex (MHC) class II molecules is optimal at mildly acidic pH. X-ray crystal structures solved for the murine class II molecule I-E(k) revealed an interesting localization of negatively charged residues within the P6 pocket, which may have implications in the pH dependency...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(02)00010-x
更新日期：2002-06-01 00:00:00
abstract：INTRODUCTION:Previous studies have suggested the importance of somatic mutations and arginine, asparagine and lysine residues in the complementarity determining regions (CDRs) of antiphospholipid antibodies (aPL) implicated in the pathogenesis of the antiphospholipid antibody syndrome. The relative contributions of the...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(03)00100-7
更新日期：2003-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2015.03.011
更新日期：2015-09-01 00:00:00
abstract：:The peptide editor HLA-DM (DM) catalyzes the exchange of peptides bound to MHC class II molecules within antigen presenting cells by generating a "peptide-receptive" MHC class II conformation (MHC(receptive)) to which peptides readily bind and rapidly unbind. While recent work has uncovered the determinants of DM reco...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.07.001
更新日期：2009-09-01 00:00:00
abstract：:The hyper-IgE syndrome (HIES) is a rare primary immunodeficiency characterized by a highly elevated serum IgE, recurrent staphylococcal skin abscesses and cyst-forming pneumonia. Non-immunological abnormalities, including a distinctive facial appearance, hyperextensive joints, scoliosis, fracture following minor traum...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.01.010
更新日期：2010-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.01.014
更新日期：2008-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.07.009
更新日期：2006-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90047-2
更新日期：1992-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.06.022
更新日期：2020-09-01 00:00:00
abstract：:In order to improve our knowledge of the human immunoglobulin variable lambda locus (IGLV), we mapped one cosmid clone (designated as C40.2) isolated by screening a Colo320HSR genomic library. The 34 kb insert of the C40.2 clone was shown to contain six genes. One gene, IGLV2S1, belongs to the V lambda II subgroup. Fo...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90175-9
更新日期：1994-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.04.026
更新日期：2019-08-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2010-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(99)00094-2
更新日期：1999-10-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90072-2
更新日期：1989-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90088-2
更新日期：1991-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(97)00024-2
更新日期：1997-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2003.11.032
更新日期：2004-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90118-0
更新日期：1987-12-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.07.010
更新日期：2010-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.09.009
更新日期：2006-04-01 00:00:00
abstract：:The development and progression of systemic lupus erythematosus (SLE) is strongly associated with complement activation and deposition. The anaphylatoxin C3a is a product of complement activation with immunomodulatory properties, and the receptor for C3a (C3aR) is not only expressed by granulocytes and antigen present...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.12.004
更新日期：2009-04-01 00:00:00
abstract：:Ankylosing spondylitis (AS) is a chronic inflammatory arthritis that affects the spine and sacroiliac joints. It causes significant disability and is associated with a number of other features including peripheral arthritis, anterior uveitis, psoriasis and inflammatory bowel disease (IBD). Significant progress has bee...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2013.06.013
更新日期：2014-01-01 00:00:00
