Abstract:
:We have developed two distinct solid-phase immunoassays for the detection of antigen binding activity by products of antigen binding T cell hybridomas in the absence of MHC. Two suppressor T cell hybridomas studied (34s-18 and 34s-704) are specific for keyhole limpet hemocyanin, a protein antigen, and the other suppressor T cell hybridoma (51H7D) binds specifically to the arsonate hapten. We have adapted these hybridomas to growth in serum-free medium and have isolated molecules with antigen binding activity both from the cell membranes and from the culture fluid in which the cells had been grown. The antigen binding molecules (ABM) produced by the KLH-specific hybridomas bound best to native hemocyanin; binding was decreased when KLH was denatured by reduction and alkylation and no binding was found to an arthropod (Limulus) hemocyanin. The arsonate binding hybridoma, on the other hand, produced molecules specific for this hapten; they showed no capacity to bind KLH. The antigen binding molecules affinity-purified from all three T hybridomas have intact masses of either 145,000, 67,000 or 48,000 when run in SDS-PAGE under non-reducing conditions. Following reduction, ABM resolve in SDS-PAGE into a complex of polypeptide chains having apparent masses of 65,000, 56,000 and 49,000, with either a pair of bands at 26,000 and 22,000, or with a single band at 32,000, which is consistent with the size of translation products of mRNA previously isolated from these hybridomas. Two of the hybridomas, 34s-18 and 34s-704, used for isolation of antigen binding products in this study, were previously reported to lack detectable rearranged gamma or beta genes and therefore to lack expression of the alpha/beta or gamma/delta heterodimers. The antigen binding molecules react in solid-phase immunoassay with some antibodies specific for variable (first framework) region and joining (J) region peptide sequences predicted from T cell receptor gene sequence. Furthermore, the affinity-purified antigen binding molecules from mouse T cell hybridomas cross-react in ELISA with goat anti-rabbit IgG and not with protein G, thus allowing the use of these commercially available reagents in standard laboratory assays. Interestingly, ABM anchored in intact cell membranes, which could be shown to specifically bind antigen, did not cross-react with goat anti-rabbit IgG, indicating that the cross-reactive moiety is not detectable when the ABM are in this situation.(ABSTRACT TRUNCATED AT 400 WORDS)
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Bristow CL,Marchalonis JJdoi
10.1016/0161-5890(89)90043-6subject
Has Abstractpub_date
1989-07-01 00:00:00pages
611-24issue
7eissn
0161-5890issn
1872-9142journal_volume
26pub_type
杂志文章abstract::The recognition structure responsible for binding to conventional antigen on target cells has not previously been described for nonspecific cytotoxic cells (NCC) or for mammalian natural killer (NK) cells. Although several biochemical pathways may be available for initiation of the lytic cycle in NCC, evidence present...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(97)00086-2
更新日期:1997-08-01 00:00:00
abstract::Serotonin release from rat basophilic leukemia (RBL) cells, sensitized with a DNP-binding monoclonal IgE, was stimulated with solid surface (polystyrene)-bound DNP-amino acids. The stimulatory potency of DNP-amino acids was dependent on the structure of amino acid attached to DNP. Generally, DNP-amino acids with high ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(91)90134-6
更新日期:1991-06-01 00:00:00
abstract::Mutation of microphthalmia transcription factor (MITF) results in deafness, bone loss, small eyes, and poorly pigmented eyes and skin. The primary cell types affected in MITF-deficient mice are melanocytes, osteoclasts and mast cells. A search for MITF-associated proteins, using a mast cell library that was screened w...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/s0161-5890(02)00059-7
更新日期:2002-09-01 00:00:00
abstract::Pathogen-driven selection is believed to be important in the evolution and maintenance of the polymorphism of the major histocompatibility complex (MHC) genes but have been tested for very few vertebrates. In this study, we first investigate by SSCP (single strand conformational polymorphism) the diversity found at th...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.03.007
更新日期:2008-06-01 00:00:00
abstract::An attempt was made to obtain fragments containing the C gamma 2 region by selectively cleaving human Fc fragments prepared from pooled IgG at Met residues using cyanogen bromide. Based on the known locations of Met residues in the Fc regions of human IgG subclasses, fragments between Met 252 and 358, comprising the C...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(85)90052-5
更新日期:1985-12-01 00:00:00
abstract::In the present report we have determined the molecular mechanisms, which govern the expression of the human IL-10 gene when induced by the glucocorticoid Methyl-Prednisolone (MP). Treatment of cells with MP at 10(-6) M will readily induce IL-10 in CD19+ primary B cells and in a human B cell line. Analysis of the IL-10...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.02.020
更新日期:2008-06-01 00:00:00
abstract::Jackfruit lectin, jacalin, prepared from two batches of jackfruit seeds showed a different specificity in precipitating reaction in Agarose gel with various purified immunoglobulins and secretory components. Jacalin-P, extracted from jackfruit seeds from the Philippines, reacts only with serum IgA and secretory IgA of...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90169-6
更新日期:1987-11-01 00:00:00
abstract::SOCS3 is well known to negatively regulate various cytokine-mediated signaling responses, but its direct role in the expression of specific cytokines has not been clearly elucidated. To understand the role of SOCS3 in the expression of IL-4, one of the key Th2 cytokines, RBL-2H3 cells (a rat mast cell line) were engin...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.11.005
更新日期:2011-02-01 00:00:00
abstract::The A/J murine hybridoma cell line 40-150 secretes antidigoxin antibodies with high affinity for digoxin. A first-order spontaneous mutant (40-150 A2.4) produces antibodies containing a mutation at heavy chain position 94 resulting in reduced affinity for digoxin. A second-order mutant (40-150 A2.4 P.10) derived from ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(92)90010-u
更新日期:1992-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(90)90126-k
更新日期:1990-10-01 00:00:00
abstract::We report that the protein tyrosine phosphatase PTP-PEST is expressed in resting human and mouse CD4(+) and CD8(+) T cells, but not in Jurkat T leukemia cells, and that PTP-PEST protein, but not mRNA, was dramatically downregulated in CD4(+) and CD8(+) primary human T cells upon T cell activation. This was also true i...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.03.019
更新日期:2008-06-01 00:00:00
abstract::The antigenic structural features of alpha-lactalbumin have been investigated using a radioimmunoassay, peptide inhibition of the quantitative precipitin reaction, and by immunodiffusion analysis after chemical modification of the molecule. Antigenic activity (in rabbits) was localized to several peptic fragments and ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(82)90192-4
更新日期:1982-11-01 00:00:00
abstract::Many infections are associated with diabetes, as the ability of the body to fight pathogens is impaired. Recently, altered levels of defensins and polymorphism in Toll-like receptors (TLRs), two crucial components of the innate immune system, have been associated with diabetes. To study the functionality of the innate...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.04.009
更新日期:2007-02-01 00:00:00
abstract::Five murine A/J hybridomas (the 35-20 group) produce anti-digoxin antibodies that have homologous heavy and light chain immunoglobulin variable regions (VH and VL), yet differ from each other in fine specificity and affinity for digoxin and related cardiac glycosides [Mudgett-Hunter et al. Molec. Immun. 22, 477-488 (1...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(89)90126-0
更新日期:1989-04-01 00:00:00
abstract::Dendritic cells (DCs) serve to maintain peripheral tolerance under steady state conditions. Upon triggering by activation signals they initiate strong immune responses. The activation of DCs is accompanied by a rapid upregulation of proinflammatory cytokines, which were shown in other cell types to be regulated by mec...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.11.002
更新日期:2010-02-01 00:00:00
abstract::The capsid protein of hepatitis B virus (P19) is made of 183 amino acids and carries the antigenic sites of hepatitis B core antigen (HBcAg) and hepatitis B e antigen (HBeAg) on the amino-terminal domain. The carboxyl-terminal domain of P19 (amino acids 150-183) is arginine-rich (47%) and faces the interior of the nuc...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(89)90130-2
更新日期:1989-04-01 00:00:00
abstract::Properly regulated mitogen-activated protein (MAP) kinase activity is critical for normal thymocyte development. MAP kinases are activated by phosphorylation of tyrosine and threonine, and dual specificity phosphatases (DUSPs) can inactivate MAP kinases by dephosphorylating both tyrosine and threonine. However, a role...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.03.006
更新日期:2006-02-01 00:00:00
abstract::A new sulfhydryl redox model of antibody-dependent endocytosis is proposed. This model synthesizes many previous studies employing a variety of experimental approaches. Predictions of this model can be tested experimentally. ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(85)90088-4
更新日期:1985-08-01 00:00:00
abstract::Following measles virus (MV) infection, viral peptides are presented to CTL by MHC class I molecules on infected antigen presenting cells at widely different epitope densities. Whereas three MV epitopes (MV-M(211-219), MV-F(438-446) and MV-H(30-38)) derived from different structural proteins occur at regular densities...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(02)00204-3
更新日期:2003-01-01 00:00:00
abstract:OBJECTIVE:Spinal cord injury (SCI) is a neurological disease with high incidence and disability. In this paper, we made a foundational study in long non-coding RNA paternally expressed gene 10 (lncRNA PEG10) at PC-12 cells. METHODS:We used CCK8, flow cytometry, migration and invasion assay to detect changes at the cel...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.11.012
更新日期:2020-02-01 00:00:00
abstract::The nature of the endogenous ligands for natural killer T (NKT) cells has been debated for more than a decade. Because the mammalian glycosylceramide synthases are invertases, it is believed that in mammals all glycosylceramides are β anomers. However, the possibility that an alternative enzymatic pathway, an unfaithf...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2015.06.009
更新日期:2015-12-01 00:00:00
abstract::Staphylococcus aureus is an etiological agent of human and animal diseases, and it is able to internalize into non-professional phagocytic cells (i.e. bovine mammary epithelial cells, bMECs), which is an event that is related to chronic and recurrent infections. bMECs contribute to host innate immune responses (IIR) t...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2015.09.025
更新日期:2015-12-01 00:00:00
abstract::In human peripheral blood, most of the CD3+ cells express the alpha/beta T cell receptor. A smaller fraction of CD3+ T cells express the gamma/delta T cell receptor (from 1 to 10% depending the individuals, with an average of 3-5%). Interestingly, although the alpha/beta + T cells never express the gamma chain at the ...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/0161-5890(92)90001-e
更新日期:1992-04-01 00:00:00
abstract::Linear IgE-binding epitopes identified in legumin allergens of peanut (Ara h 3) and other allergenic tree nuts (Jug r 4 of walnut, Cor a 9 of hazelnut, Ana o 2 cashew nut) were mapped on three-dimensional models of the proteins built up by homology modelling. A conformational analysis revealed that consensual surface-...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.01.023
更新日期:2007-05-01 00:00:00
abstract::Programmed cell death protein 1 (PD1) is a cell-surface receptor that plays a vital regulatory role in suppressing inflammatory T cell activity; therefore, it is an ideal target for T cell-redirecting therapies. Here, we describe a cynomolgus macaque model for studying the transfer of PD1-modified T cells. We develope...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.05.007
更新日期:2020-08-01 00:00:00
abstract::The aim of the study was to explore whether fever-range hyperthermia (FRH) might enhance the anticancer and immunoregulatory activities of protein-bound polysaccharides (PBP), a class of fungus derived immunomodifiers used in the cancer adjuvant therapy. Blood lymphocytes and breast cancer cells (MCF-7) were cultured ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.10.013
更新日期:2016-12-01 00:00:00
abstract::Complement factor H (CFH) protein is an inhibitor of the alternative pathway of complement (AP) both in the fluid phase and on the surface of host cells. Mouse and human complement factor H-related (CFHR) proteins also belong to the fH family of plasma glycoproteins. The main goal of the current study was to compare t...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2014.06.028
更新日期:2014-11-01 00:00:00
abstract::Interferon (IFN)-γ is highly upregulated during heart inflammation and enhances the production of pro-inflammatory cytokines. Absent in Melanoma 2 (AIM2) is an IFN-inducible protein implicated as a component of the inflammasome. Here we seek to determine the role of AIM2 during inflammation in cardiac cells. We found ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.04.009
更新日期:2016-06-01 00:00:00
abstract::A first report of an XLA patient with a polymorphism in Btk SH3 domain has been identified after sequencing of the entire gene. SH3 domain variants might not be detected due to well characterized mutations outside the domain. ...
journal_title:Molecular immunology
pub_type: 信件
doi:10.1016/j.molimm.2007.07.005
更新日期:2008-01-01 00:00:00
abstract::This study was undertaken to investigate the nature and microheterogeneity of the carbohydrate moiety of the Fc epsilon receptors of RBL-CA10 and RBL-CA10.7 cells. Treatment using the glycosylation processing inhibitors, castanospermine (CN), 1-deoxymannojirimycin (DMJ), and swainsonine (SW) resulted in a decrease of ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90060-o
更新日期:1993-02-01 00:00:00