Abstract：
:This study was undertaken to investigate the nature and microheterogeneity of the carbohydrate moiety of the Fc epsilon receptors of RBL-CA10 and RBL-CA10.7 cells. Treatment using the glycosylation processing inhibitors, castanospermine (CN), 1-deoxymannojirimycin (DMJ), and swainsonine (SW) resulted in a decrease of the relative molecular mass (M(r)) of both the alpha-chain of the high affinity receptor for IgE, Fc epsilon RI(alpha), and the low affinity receptor for IgE, Fc epsilon RL. Exposure to DMJ had the greatest effect on the M(r), while CN seemed to lead to a decreased cell surface expression of Fc epsilon RI. Both receptors are largely resistant to endoglycosidase H as their M(r) decreased only by approximately 2 kDa. These results suggest that both receptors are composed primarily of complex oligosaccharides with a single high mannose, N-glycosylated site. Both Fc epsilon receptors become endoglycosidase H sensitive if first exposed to DMJ which indicates that the carbohydrate composition is indeed altered by this processing inhibitor presumably by blocking the formation of complex structures. When the Fc epsilon receptors were reduced and hydrolyzed by N-glycanase, the M(r) values for Fc epsilon RI(alpha) and Fc epsilon RL decreased to approximately 28 and 34-38 kDa respectively. In the case of Fc epsilon RI(alpha), this implies the presence of only a small amount of O-linked oligosaccharides.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
LaCroix EL,Froese Adoi
10.1016/0161-5890(93)90060-osubject
Has Abstractpub_date
1993-02-01 00:00:00pages
321-30issue
3eissn
0161-5890issn
1872-9142journal_volume
30pub_type
杂志文章abstract：:The dynamics of human antigen-specific immunoglobulin (Ig) responses in early life are not well characterized. We have previously observed an inverse relationship between allergen-specific Ig concentration and allergen-Ig-binding affinity in allergen-sensitive atopic adults, suggesting a possible feedback relationship...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.07.006
更新日期：2005-01-01 00:00:00
abstract：:A panel of human T cell clones specific for measles virus was characterized and among them fusion protein-specific, DR1-and DP-restricted T cell clones were selected to study the processing and presentation of determinants borne by a viral membrane protein. Using two independent methods to assess the activation of T c...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00148-4
更新日期：1996-03-01 00:00:00
abstract：:The number of V alpha and V beta sequences of T cell receptors now available allows a meaningful analysis of their variability profiles. Variability plots were derived using a modified form of Wu and Kabat's algorithm: variability is not computed as a proportion of the number of different residues occurring at a posit...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90178-m
更新日期：1991-09-01 00:00:00
abstract：:Bla g 1 is a major allergen from Blatella germanica and one of the primary allergens used to assess cockroach allergen exposure. The epitope of an anti-Bla g 1 scFv was mapped in order to better understand cross reactivity with other group 1 cockroach allergens and patient IgE epitopes. X-ray crystallography was used ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.02.003
更新日期：2014-06-01 00:00:00
abstract：BACKGROUND:Ethanol feeding in mice activates complement via C1q binding to apoptotic cells in the liver; complement contributes to ethanol-induced inflammation and injury. Despite the critical role of C1q in ethanol-induced injury, the mechanism by which ethanol activates C1q remains poorly understood. Secretory IgM (s...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.02.008
更新日期：2016-04-01 00:00:00
abstract：:Affinity-purified major histocompatibility complex (MHC) class II molecules are known to bind antigenic peptides in vitro. The percentage of MHC class II molecules occupied with such peptides is usually very low and varies significantly depending upon the sequence and size of a given antigenic peptide. The present stu...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90030-2
更新日期：1994-10-01 00:00:00
abstract：:Pathogen-driven selection is believed to be important in the evolution and maintenance of the polymorphism of the major histocompatibility complex (MHC) genes but have been tested for very few vertebrates. In this study, we first investigate by SSCP (single strand conformational polymorphism) the diversity found at th...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.03.007
更新日期：2008-06-01 00:00:00
abstract：:In addition to classic Smad signaling pathways, the pleiotropic immunoregulatory cytokine TGF-beta1 can activate MAP kinases, but a role for TGF-beta1-MAP kinase pathways in T cells has not been defined heretofore. We have shown previously that TGF-beta1 inhibits Th1 development by inhibiting IFN-gamma's induction of ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.02.024
更新日期：2007-07-01 00:00:00
abstract：:Interleukin 1 receptor-associated kinase 4 (IRAK4) is a key factor in TLR-mediated host immune function. In this study, an IRAK4 homologue molecule was identified from Apostichopus japonicus (designated as AjIRAK4) by RACE approach. The full-length cDNA of AjIRAK4 was of 2024 bp containing an open reading frame of 131...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2018.08.006
更新日期：2018-09-01 00:00:00
abstract：:The purpose of the present work was to determine the ability of BgTX peptides, corresponding to the various loops and exposed regions of alpha-bungarotoxin (BgTX) and representing regions that are recognized by B and/or T cells, to stimulate protective immunity in mice against in vivo challenge with BgTX. The BgTX LD5...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(96)00014-4
更新日期：1996-05-01 00:00:00
abstract：:In this study, a novel approach for the development of a peptide-based vaccine has been tested. We investigated the possibility of replacing an all-L amino acid peptide sequence corresponding to the protective B-cell epitope hemagglutinin (HA) 91-108 from influenza HA with a retro-inverso analogue encompassing this se...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(02)00095-0
更新日期：2002-10-01 00:00:00
abstract：:Four monoclonal antibodies (MAbs) directed against the P1 blood group antigen were produced by hybridomas obtained from mouse immunized with turtle-dove avomucoid. One of the MAb (154 IX B6) selected as a blood typing reagent agglutinated native P1 and Pk1 red cells with a high titer but was inactive against native P2...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90089-7
更新日期：1987-02-01 00:00:00
abstract：:Tremendous advances in our understanding of the thrombotic microangiopathies (TMAs) have revealed distinct disease mechanisms within this heterogeneous group of diseases. As a direct result of this knowledge, both children and adults with complement-mediated TMA now enjoy higher expectations for long-term health. In t...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2015.03.246
更新日期：2015-09-01 00:00:00
abstract：:Histocompatibility Leukocyte Antigens, or HLAs, are one of the most polymorphic molecules in humans. This high degree of polymorphism endows HLA molecules with the ability to present a vast array of peptides, an essential trait for responding to ever-evolving pathogens. Unlike classical HLA molecules (HLA-Ia), some no...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2020.02.011
更新日期：2020-04-01 00:00:00
abstract：:Despite the fact that was one of the first systems to be discovered and investigated in the innate immunity, Complement is continuing to receive growing attention by the scientific community. Complement is involved in several diseases such as diabetes, atherosclerosis or Systemic Lupus Erythematous. Successful therape...
journal_title：Molecular immunology
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.molimm.2015.06.003
更新日期：2015-11-01 00:00:00
abstract：:The hyper-IgE syndrome (HIES) is a rare primary immunodeficiency characterized by a highly elevated serum IgE, recurrent staphylococcal skin abscesses and cyst-forming pneumonia. Non-immunological abnormalities, including a distinctive facial appearance, hyperextensive joints, scoliosis, fracture following minor traum...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.01.010
更新日期：2010-04-01 00:00:00
abstract：:Atypical hemolytic uremic syndrome (aHUS) is a disease of complement dysregulation, characterized by hemolytic anemia, thrombocytopenia and acute renal failure. Mutations in complement inhibitors are major risk factors for development of aHUS. The three aHUS patients reported in this study had several previously ident...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2015.02.013
更新日期：2015-06-01 00:00:00
abstract：:Anti-DNP activity was found in normal human immunoglobulins with average affinities (10(4)-10(6) l/mole) and frequencies (15-2%) that correlate well with those expected from multispecific binding sites. ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(84)90143-3
更新日期：1984-10-01 00:00:00
abstract：:Three interferon regulatory factor (IRF) genes, CaIRF-1, CaIRF-2 and CaIRF-7, and their promoters of snakehead (Channa argus) were cloned and characterized. The CaIRF-1 gene consists of ten exons, spans 4.3 kb and encodes a putative peptide of 299 aa. The CaIRF-2 gene consists of nine exons, spans 8 kb and encodes a p...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.11.011
更新日期：2008-04-01 00:00:00
abstract：:C3 glomerulopathy (C3G) is an ultra-rare complement-mediated renal disease characterized histologically by the predominance of C3 deposition within in the glomerulus. Familial cases of C3G are extremely uncommon and offer unique insight into the genetic drivers of complement dysregulation. In this report, we describe ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.07.007
更新日期：2016-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(03)00102-0
更新日期：2003-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(96)84614-1
更新日期：1996-07-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.02.024
更新日期：2007-01-01 00:00:00
abstract：:Polymorphonuclear cells (PMN) are widely recognized as sophisticated killers during microbial infections. In recent years, PMN have been shown to interact and functionally interfere with other cells of the immune system. In this study, we investigated PMN-T cell interactions in an in vitro co-culture system. A relativ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.07.006
更新日期：2011-09-01 00:00:00
abstract：:The A/J murine hybridoma cell line 40-150 secretes antidigoxin antibodies with high affinity for digoxin. A first-order spontaneous mutant (40-150 A2.4) produces antibodies containing a mutation at heavy chain position 94 resulting in reduced affinity for digoxin. A second-order mutant (40-150 A2.4 P.10) derived from ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90010-u
更新日期：1992-04-01 00:00:00
abstract：:The fine regulation of NF-kappaB activity is crucial for both resting and stimulated cells and relies on complex balance between multiple activators and inhibitors. We report here that c-mip, a recently identified pleckstrin homology (PH) and leucine-rich repeat (LRR)-domain-containing protein, inactivates GSKbeta and...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.09.034
更新日期：2009-02-01 00:00:00
abstract：:Dehydroepiandrosterone (DHEA) has anti-inflammatory, anti-oxidant and immune-regulating properties, while the mechanism of DHEA actions remains unclear. The present study aims to investigate the effect and possible mechanism of DHEA on immune function of mice in vivo and in vitro. In vivo, a lipopolysaccharide (LPS)-i...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.06.004
更新日期：2019-08-01 00:00:00
abstract：:In an attempt to understand the relationship of amino acid sequence to the formation of primary or multiple myeloma-related amyloid (AL amyloid), we have determined the complete amino acid sequence of amyloid protein BAN. This protein belongs to the kappa I immunoglobulin light chain subgroup and has a polypeptide cha...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90173-2
更新日期：1986-01-01 00:00:00
abstract：:Monoclonal antibodies were prepared against synthetic peptides synthetized on the basis of nucleotide sequence from cDNA and genomic clones encoding a class I antigen expressed by the rabbit RL-5 cell line. Using a peptide corresponding to positions 61-73 of the N domain, we were able to obtain an hybridoma producing ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90019-8
更新日期：1987-05-01 00:00:00
abstract：:Properly regulated mitogen-activated protein (MAP) kinase activity is critical for normal thymocyte development. MAP kinases are activated by phosphorylation of tyrosine and threonine, and dual specificity phosphatases (DUSPs) can inactivate MAP kinases by dephosphorylating both tyrosine and threonine. However, a role...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.03.006
更新日期：2006-02-01 00:00:00
