Abstract：
:The purpose of the present work was to determine the ability of BgTX peptides, corresponding to the various loops and exposed regions of alpha-bungarotoxin (BgTX) and representing regions that are recognized by B and/or T cells, to stimulate protective immunity in mice against in vivo challenge with BgTX. The BgTX LD50 values in non-immune mice or mice that had been immunized with proteins and peptides unrelated to BgTX were: Balb/c, 0.128 microgram/g; SJL, 0.156 microgram/g. Immunization of Balb/c and SJL mice with each of the synthetic peptides in its free form afforded considerable protection against BgTX poisoning. Peptides L1 (residues 3-16), L2 (residues 26-41) and C-tail (residues 66-74) of BgTX were the most protective and mice immunized with these peptides survived LD50 values that were three times higher than control mice. Immunization with an equimolar mixture of the three peptides was even more protective and these mice survived even higher challenge doses of BgTX (4.6-fold higher than LD50 of controls; i.e. protection index, PI = 4.6). An OVA conjugate carrying all three peptides, when used as an immunogen, conferred extremely high protection (PI > or = 18.1) which was almost double the protection obtained by BgTX immunization (PI = 9.7). Thus, the conjugate of the three peptides should serve as an effective vaccine against BgTX poisoning. Furthermore, these results with BgTX peptides should serve as a prototype for the design and synthesis of peptide vaccines against other members of this large family of toxins which include both long and short neurotoxins as well as cytotoxins.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Dolimbek BZ,Atassi MZdoi
10.1016/0161-5890(96)00014-4subject
Has Abstractpub_date
1996-05-01 00:00:00pages
681-9issue
7-8eissn
0161-5890issn
1872-9142journal_volume
33pub_type
杂志文章abstract：:The primary immune response against Staphylococcus aureus is mediated by neutrophils. In response to S. aureus and its proteins, neutrophil shows two different kinds of NETosis, viz. suicidal and vesicular NETosis. Glucose is the major energy source of neutrophils for performing NETosis. However, NETosis was found alt...
journal_title：Molecular immunology
pub_type： 杂志文章
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.01.014
更新日期：2008-05-01 00:00:00
abstract：:Lysozyme is an important component of the insect non-specific immune response against bacteria that is characterized by its ability to break down bacterial cell-walls. By searching an EST database from the fall armyworm, Spodoptera frugiperda (Negre et al., 2006), we identified five sequences encoding proteins of the ...
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pub_type： 杂志文章
doi：10.1016/j.molimm.2009.09.028
更新日期：2009-12-01 00:00:00
abstract：:Conserved extended haplotypes (CEHs) are large (>or=1Mb) regions of identical DNA of the major histocompatibility complex (MHC) region of chromosome 6p in unrelated individuals. They are recognized by family studies and constitute nearly half of MHC haplotypes among European Caucasians. We studied 49 Hungarian Caucasi...
journal_title：Molecular immunology
pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1016/0161-5890(82)90195-x
更新日期：1982-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00148-4
更新日期：1996-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：1989-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0161-5890(84)90143-3
更新日期：1984-10-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)00160-3
更新日期：1995-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.01.010
更新日期：2005-08-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90139-6
更新日期：1983-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90099-j
更新日期：1992-02-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90069-n
更新日期：1993-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00107-7
更新日期：1996-02-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.09.017
更新日期：2006-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
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更新日期：2019-08-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(01)00060-8
更新日期：2001-08-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/s0161-5890(03)00110-x
更新日期：2003-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.11.005
更新日期：2011-02-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90042-6
更新日期：1994-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(02)00010-x
更新日期：2002-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)00150-y
更新日期：1995-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(84)90142-1
更新日期：1984-10-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.12.021
更新日期：2017-02-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(96)00021-1
更新日期：1996-05-01 00:00:00
abstract：OBJECTIVE:To investigate the impact of death-associated protein kinase 1 (Dapk1) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) via p38MAPK/NF-κB pathway. METHODS:Dapk1+/+ and Dapk1-/- mice were randomized into Control, LPS, SB203580 (a p38MAPK pathway inhibitor) + LPS, and PDTC (a NF-κB pathway inhibitor...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.01.014
更新日期：2020-04-01 00:00:00
abstract：:Crotoxin (Ctx) is the main lethal component of Crotalus durissus terrificus venom. It is a neurotoxin, composed of two subunits associated by noncovalent interactions, the non-toxic acid subunit (CA), named Crotapotin, and the basic subunit (CB), with phospholipase A2 (PLA2) activity. Employing the SPOT synthesis tech...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.01.012
更新日期：2020-03-01 00:00:00
abstract：:TRAF6 is a key player at the cross-roads of development and immunity. The analysis of its in vivo molecular function is a great challenge since severe developmental defects and early lethality caused by Traf6 deficiency in knock-out mice interfere with analyses of the immune response. In this study we have used a new ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.08.011
更新日期：2010-11-01 00:00:00
