Abstract:
:In addition to the antigen receptor, resting T cells express a number of receptors that can be stimulated to generate proliferative signals. These "accessory" receptors require co-expression of the T cell receptor (TCR), suggesting that they channel their signals via secondary activation of the signal transduction function of the CD3-TCR complex. Little is known about how different receptors control each other's function when one or more stimuli are presented at the same time. In order to study the regulation of accessory receptors by the CD3-TCR and vice versa, we have investigated the activation of the CD2 cell adhesion molecule receptor and the pertussis toxin receptor, a 43 kDa plasma membrane protein. Both receptors can activate signal transduction pathways in T cells similar to that of the CD3-TCR, including increases in Ca2+ and phosphatidylinositol turnover. They are also similar in that they utilize the antigen receptor to transmit their signals to the cell since CD3-TCR(-) mutants cannot be activated via either CD2 or the toxin receptor. We have previously shown that submaximal stimulation of the CD3-TCR blocks second messenger generation and proliferation in response to pertussis toxin. This heterologous desensitization was unidirectional since activation of the toxin receptor had no effect on CD3-TCR function. Here we extend these studies to show that activation of both CD2 and the toxin receptor led to rapid tyrosine phosphorylation of three similar proteins. Submaximal stimulation of the CD3-TCR completely inhibited toxin receptor-stimulated tyrosine protein kinase activity but did not desensitize CD2 function as determined by activation of tyrosine protein phosphorylation. Furthermore, CD2 stimulation did not lead to desensitization of the pertussis toxin receptor. These data support a system of complex regulatory relationships between different signaling receptors and suggest a model for signal integration and inter-receptor cross-talk in T cell activation.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Zhang XM,Berland R,Rosoff PMdoi
10.1016/0161-5890(94)00160-3subject
Has Abstractpub_date
1995-04-01 00:00:00pages
323-32issue
5eissn
0161-5890issn
1872-9142pii
0161589094001603journal_volume
32pub_type
杂志文章abstract::We have determined the cDNA sequence encoding J chain, a polypeptide accessory molecule associated with polymeric Ig, from the anuran amphibian, Xenopus laevis (South African clawed frog). The translated polypeptide consists of 164 amino acid residues, including the signal sequence, and is somewhat longer than the cor...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(97)82768-x
更新日期:1997-10-01 00:00:00
abstract::Two anti-phenyloxazolone (phOx3) and one anti-GAT MAbs from C57BL mice are shown to be coded by VH gene 186.2. This gene has been found earlier to code for several anti-NP (NNP) antibodies (Bothwell et al., 1981) and anti-GT antibodies (Rocca-Serra et al., 1983; Carmack and Pincus, 1986). The L chain partner of the VH...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(88)90122-8
更新日期:1988-09-01 00:00:00
abstract::The RING finger type E3 ubiquitin ligase, Cbl-b, is abundantly expressed in bone marrow-derived mast cells (BMMCs) and functions as a potent negative regulator of signalling responses from the high-affinity IgE receptor (FcvarepsilonRI). To determine the contribution of Cbl-b E3 ligase activity we generated knockin mi...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.08.002
更新日期:2008-02-01 00:00:00
abstract::Human cell lines and blood lymphocytes were treated for short time periods with IFN-gamma. This treatment increased the amount of the assembled MHC class I molecules on the plasma membrane after 30 min. This early increase of the membrane expression subsided in the next few hours. A second wave of elevation occurred a...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90008-y
更新日期:1993-06-01 00:00:00
abstract::The mammalian interleukin (IL)-6 family of cytokines consist of eight class-I helical cytokines and are major players in hematopoiesis, as well as in neuroendocrine and immune systems, and have pro- and anti-inflammatory properties. We have identified a novel fish CNTF-like molecule, for the first time, as well as the...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.04.003
更新日期:2009-07-01 00:00:00
abstract::The effectiveness of immunotherapy targeting the immune checkpoint PD-L1/PD-1 pathway highlights importance of elucidating the regulatory mechanisms of PD-L1 expression in cancer cells. Previous studies demonstrate that oncogene MYC up-regulates PD-L1 expression in lymphomas. In the present study, we investigated the ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2018.07.006
更新日期:2018-09-01 00:00:00
abstract::GRX1 (glutaredoxin1), a sulfhydryl disulfide oxidoreductase, is involved in many cellular processes, including anti-oxidation, anti-apoptosis, and regulation of cell differentiation. However, the role of GRX1 in the oxidative stress and apoptosis of osteoarthritis chondrocytes remains unclear, prompting the current st...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.08.006
更新日期:2017-10-01 00:00:00
abstract::Renal transplantation is the treatment of choice for patients with end-stage renal disease. The vital role of the complement system in renal transplantation is widely recognized. This review discusses the role of complement in the different phases of renal transplantation: in the donor, during preservation, in reperfu...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2017.05.014
更新日期:2017-09-01 00:00:00
abstract::Asthma is a complex condition in which exposure to environmental antigens induces inflammatory reactions in the airway characterized by activation of mast cells and eosinophils. Mast cells are known to be the main effector cells in eliciting IgE-mediated allergic response. These cells secrete various substances that p...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/s0161-5890(02)00070-6
更新日期:2002-09-01 00:00:00
abstract::The general thinking about the phylogenic distribution of vertebrate Ig classes is that fish and urodele amphibians are only able to synthesize polymeric IgM-like molecules and that the emergence of a new class of LMW Ig occurs for the first time in anouran species. Following immunization of the Mexican axolotl (Ambys...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(88)90054-5
更新日期:1988-12-01 00:00:00
abstract::The innate immune system is the first line of defense against infection and responses are initiated by pattern recognition receptors (PRRs) that detect pathogen-associated molecular patterns (PAMPs). PRRs also detect endogenous danger-associated molecular patterns (DAMPs) that are released by damaged or dying cells. T...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2017.02.012
更新日期:2017-06-01 00:00:00
abstract::Using the non-crossreactive mAb MRC-OX3 and MRC-OX6, two serologically distinct RT1.B-specific (I-A equivalent) alpha, beta heterodimers have previously been described by us as residing at the cell surface of LEW rat spleen cells. The two-chain elements were suggested to represent stable conformation isomers, diverged...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(89)90099-0
更新日期:1989-02-01 00:00:00
abstract::Most studies of the immune responses in allergic rhinitis have focused on IgE antibodies to mixtures of allergenic proteins. Based on our previous studies of the major mountain cedar allergen Jun a 1, we sought to describe a broader assessment of the humoral immune responses to a single, dominant allergen, in three gr...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2018.08.007
更新日期:2018-09-01 00:00:00
abstract::Previously we reported that, early in the antibody response of BALB/c mice to several cytochromes c (CYT) coupled to ovalbumin (OVA), B cells responding to the self antigen mouse CYT recognized a single site on mouse CYT and were in much higher frequency than B cells responding to foreign CYT. In the present study the...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(97)00109-0
更新日期:1997-08-01 00:00:00
abstract::This study analyzes the gene repertoire coding for antibodies to an evolutionary novel immunogenic carbohydrate antigen in mice. The alpha-gal epitope (Gal alpha 1-3Gal beta 1-4GlcNAc-R) is an autoantigen, abundantly expressed in wild type mice, but absent in alpha 1,3galactosyltransferase knock-out (KO) mice, where i...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(00)00064-x
更新日期:2000-06-01 00:00:00
abstract::An attempt was made to obtain fragments containing the C gamma 2 region by selectively cleaving human Fc fragments prepared from pooled IgG at Met residues using cyanogen bromide. Based on the known locations of Met residues in the Fc regions of human IgG subclasses, fragments between Met 252 and 358, comprising the C...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(85)90052-5
更新日期:1985-12-01 00:00:00
abstract::PIM1 is serine/threonine protein kinase that is involved in numerous biological processes. Pulmonary fibrosis (PF) is a chronic pathological result of the dysfunctional repair of lung injury without effective therapeutic treatments. In the current study, we investigated whether PIM1 inhibition would improve bleomycin ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.06.013
更新日期:2020-09-01 00:00:00
abstract::Activation of p38 MAP kinase in T cells leads to increased interferon-gamma production in CD4+ and CD8+ T cells, and the selective cell death of CD8+ T cells. To address the role of p38 MAP kinase activation in T cells during an in vivo immune response, we examined the response against the influenza virus in transgeni...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(00)00078-x
更新日期:2000-06-01 00:00:00
abstract:OBJECTIVE:Nuclear components targeted by autoantibodies are a characteristic feature of the autoimmune disease systemic lupus erythematosus (SLE). The nucleosome, a major autoantigen, is released in patients with SLE as a result of a disturbed apoptosis and/or an insufficient clearance of apoptotic debris. During apopt...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.08.003
更新日期:2010-11-01 00:00:00
abstract::Fibroblast-like synoviocyte (FLS) is the ultimate effectual cells in the pathogenesis of rheumatoid arthritis (RA). The current study was undertaken to investigate whether a proliferation-inducing ligand (APRIL) mediates the function of FLS in an animal model of RA. Rat adjuvant-induced arthritis (AA) was induced by i...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2014.11.002
更新日期:2015-03-01 00:00:00
abstract::Individually amplified kappa cDNA molecules from single B lymphocytes revealed sequence heterogeneity and aberrantly spliced products. The nature and frequency of the base changes and their absence from similarly amplified beta2 microglobulin transcripts indicate that they were not derived by Taq polymerase misincorpo...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(00)00055-9
更新日期:2000-04-01 00:00:00
abstract::IFN-gamma plays an essential role in the IL-12/IL-23/IFN-gamma pathway that is required for the defense against intracellular pathogens. In the IFN-gammaR1 several amino acid substitutions have been reported that abrogate IFN-gamma signaling. These substitutions can lead to a null phenotype and enhanced susceptibility...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.11.016
更新日期:2010-02-01 00:00:00
abstract::The T-dependent BALB/c murine immune response to fluorescein (F1) is characterized by structural heterogeneity at the protein level exemplified in part by a significantly wide range of affinities (Ka), and apparent lack of dominant idiotypes. In order to generate an idiotype family, xenogenic anti-idiotype (anti-ID) a...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(85)90072-0
更新日期:1985-08-01 00:00:00
abstract::Prohibitin (PHB1) is a multifunction protein that is released in lipid droplets from adipocytes and possibly other cells and is detectable in the circulation. We used crosslinking, immunoprecipitation and proteomic analysis to investigate binding partners for circulating PHB1. Crosslinking of PHB1 to serum resulted in...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.09.025
更新日期:2007-03-01 00:00:00
abstract::To date, although a promising anti-inflammatory activity of oligomeric proanthocyanidins (OPCs) has been observed in asthma, the mechanism responsible for these immunomodulatory properties remains obscure. Dendritic cells (DCs) that reside in the airway have been widely perceived as an important contributor to asthma....
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.09.012
更新日期:2017-11-01 00:00:00
abstract::We have designed a 39 amino acid peptide mimic of the conformation-dependent main immunogenic region (MIR) of the Torpedo acetylcholine receptor (TAChR) that joins three discontinuous segments of the Torpedo α-subunit, α(1-12), α(65-79), and α(110 - 115) with two GS linkers: This 39MIR-mimic was expressed in E. coli a...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2014.01.002
更新日期:2014-05-01 00:00:00
abstract::Idiotype (Id) and autoanti-thyroglobulin were induced in different strains of mice by priming with anti-Id to monoclonal anti-thyroglobulins (D8 and G4) and challenged with a subimmunogenic dose of thyroglobulin (Tg). Both D8.Id and G4.Id were induced in CBA mice by priming with the appropriate anti-Id, but only primi...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(85)90159-2
更新日期:1985-03-01 00:00:00
abstract::Adiponectin can protect against inflammation; one of the mechanisms involves direct, inhibition of macrophages (MΦ). We postulated that adiponectin anti-sense transgenic (AsTg) mice raised in our laboratory are prone to inflammation because of systemic low adiponectin levels. The writhing response to acetic acid was u...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2013.08.015
更新日期:2014-02-01 00:00:00
abstract::Bothropasin is a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops jararaca venom, the snake responsible for most bites in Southeastern Brazil. SVMPs, such as bothropasin, are involved in the main bothropic envenoming symptoms, which include hemorrhage, inflammation, necrosis and blood coagulation deficie...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2018.10.019
更新日期:2018-12-01 00:00:00
abstract::Fibrates, which function by binding and activating peroxisome proliferator-activated receptor alpha (PPARalpha), have been used successfully to treat hyperlipidemia and atherosclerosis. Increasing evidence suggests that in addition to their lipid lowering activities these medications also function as immunosuppressive...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.06.006
更新日期:2007-02-01 00:00:00