Abstract：
:Individually amplified kappa cDNA molecules from single B lymphocytes revealed sequence heterogeneity and aberrantly spliced products. The nature and frequency of the base changes and their absence from similarly amplified beta2 microglobulin transcripts indicate that they were not derived by Taq polymerase misincorporations or by a general infidelity in RNA polymerase. The trinucleotide sequences in which the base changes occurred are disfavored targets of the somatic hypermutation mechanism that modifies antibody variable (V) region genes during immunity. Taken together with the observation that the transcript alterations were absent from the kappa Ig gene, this suggests that somatic mutations were acquired by the kappa gene and rapidly repaired following limited transcription. Preferential repair of mutations located in specific trinucleotide contexts could be the basis for some of the microsequence-specific bias in mutation frequencies observed in antibody V region genes.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Jena PK,Liu AH,Smith DS,Aviszus K,Wysocki LJdoi
10.1016/s0161-5890(00)00055-9subject
Has Abstractpub_date
2000-04-01 00:00:00pages
265-72issue
6eissn
0161-5890issn
1872-9142pii
S0161589000000559journal_volume
37pub_type
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pub_type： 杂志文章,评审
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