Abstract：
:TRAF6 is a key player at the cross-roads of development and immunity. The analysis of its in vivo molecular function is a great challenge since severe developmental defects and early lethality caused by Traf6 deficiency in knock-out mice interfere with analyses of the immune response. In this study we have used a new strategy to analyze the function of Traf6 in a zebrafish-Salmonella infectious disease model. In our approach the effect of a Traf6 translation-blocking morpholino was titrated down to avoid developmental defects and the response to infection under these conditions was studied using the combination of microarray analysis and whole transcriptome deep sequencing. Transcriptome profiling of the traf6 knock-down allowed the identification of a gene set whose responsiveness during infection is highly dependent on Traf6. Expression trend analysis based on the resulting datasets identified nine clusters of genes with characteristic transcription response profiles, demonstrating Traf6 has a dynamic role as a positive and negative regulator. Among the Traf6-dependent genes was a large set of well known anti-microbial and inflammatory genes. Additionally, we identified several genes which were not previously linked to a response to microbial infection, such as the fertility hormone gene gnrh2 and the DNA-damage regulated autophagy modulator 1 gene dram1. With the use of the zebrafish embryo model we have now analyzed the in vivo function of Traf6 in the innate immune response without interference of adaptive immunity.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Stockhammer OW,Rauwerda H,Wittink FR,Breit TM,Meijer AH,Spaink HPdoi
10.1016/j.molimm.2010.08.011subject
Has Abstractpub_date
2010-11-01 00:00:00pages
179-90issue
1-3eissn
0161-5890issn
1872-9142pii
S0161-5890(10)00555-9journal_volume
48pub_type
杂志文章abstract：:Rainbow trout (Oncorhynchus mykiss) cells of a monocyte-macrophage lineage (rtMOCs) were used to characterize the ability of the trout innate immune system to recognize and respond to different pathogen associated molecular pattern (PAMP) bearing substances. Compared to what has been reported for mammalian macrophages...
journal_title：Molecular immunology
pub_type： 杂志文章
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journal_title：Molecular immunology
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doi：10.1016/j.molimm.2007.10.024
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abstract：:UL16 binding proteins (ULBPs) are ligands for the NK cell activating receptor NKG2D. A cDNA encoding a porcine ULBP-like protein (PULBP) was cloned and the predicted amino acid sequence exhibited 35-52% identity to human ULBPs. Southern blot analysis suggested that there is only one ULBP-like gene in the pig genome. T...
journal_title：Molecular immunology
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更新日期：2005-04-01 00:00:00
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pub_type： 杂志文章
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更新日期：1987-12-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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abstract：:TNFα converting enzyme (TACE) is responsible for converting membrane-anchored TNFα to its soluble form in mammalian. However, the function and characteristics of TACE in teleosts is unclear. In this study, we report the cloning of a cDNA sequence of the PaTACE from ayu, Plecoglossus altivelis. PaTACE encodes an 865-aa...
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：1994-10-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2007-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：1986-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2019-12-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.07.010
更新日期：2018-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90131-3
更新日期：1987-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/s0161-5890(01)00123-7
更新日期：2002-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.09.004
更新日期：2010-11-01 00:00:00
abstract：:C-reactive protein (CRP) is a pattern recognition molecule that binds several microbial and host ligands. Ligand-bound CRP activates the complement system via the classical pathway. Previously, we identified human complement factor H-related protein 4 (CFHR4), a member of the factor H protein family, as a CRP binding ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.12.005
更新日期：2010-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 临床试验,杂志文章
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更新日期：2015-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.09.020
更新日期：2006-03-01 00:00:00
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journal_title：Molecular immunology
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更新日期：2008-11-01 00:00:00
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更新日期：2000-12-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2019-10-01 00:00:00
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pub_type： 杂志文章
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journal_title：Molecular immunology
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更新日期：2009-08-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.12.010
更新日期：2021-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0161-5890(91)90179-n
更新日期：1991-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
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更新日期：2020-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0161-5890(85)90052-5
更新日期：1985-12-01 00:00:00
abstract：:alpha 1-Microglobulin (alpha lm), a glycoprotein heterogeneous in charge, was reported to occur both as a 31-kilodalton (kd) monomer [low mol. wt alpha lm (LMW-alpha lm)] and as polymers or complexes formed with other plasma proteins including IgA [high mol. wt alpha lm (HMW-alpha lm)]. The present study was designed ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90096-3
更新日期：1985-06-01 00:00:00
abstract：OBJECTIVE:To investigate the impact of death-associated protein kinase 1 (Dapk1) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) via p38MAPK/NF-κB pathway. METHODS:Dapk1+/+ and Dapk1-/- mice were randomized into Control, LPS, SB203580 (a p38MAPK pathway inhibitor) + LPS, and PDTC (a NF-κB pathway inhibitor...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.01.014
更新日期：2020-04-01 00:00:00
