Abstract：
:Peptides representing the amino-terminal sequence of protamines HP1 (sequence 1-12) and HP2 (sequence 1-11), the two major nuclear proteins of human sperm, have been synthesized. Rabbits were immunized either with peptide conjugated with a carrier or with free peptide. The resulting antisera were examined for their capacities to bind the homologous peptide, other peptides from protamines HP1, HP2, from ram protamine, a protein resembling HP1, and finally with the whole protamine. Only free peptides were immunogenic. Antisera were found to react with the homologous peptide, but also with some other peptides. More especially, antibodies to peptide HP1 1-12 were found to recognize an epitope shared by the homologous peptide, peptide HP1 37-49 and peptide 1-12 of ram protamine. The common antigenic determinant seems to depend on the conformation of the peptides, rather than strictly related to common sequences. Anti-peptide antibodies react poorly and in a non-specific manner with the parent protein. The failure of reactivity with the protamines strongly suggest that these small basic proteins are folded and probably globular molecules in contrast with the totally random model postulated by several previous works.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Rousseaux-Prevost R,Hublau P,Sautiere P,Rousseaux Jdoi
10.1016/0161-5890(91)90179-nsubject
Has Abstractpub_date
1991-09-01 00:00:00pages
943-9issue
9eissn
0161-5890issn
1872-9142journal_volume
28pub_type
杂志文章abstract：:This paper describes additional structural analyses of the p24 cell surface molecule recognized by monoclonal antibody BA-2. Since BA-2 is broadly reactive with a variety of normal and malignant lymphohematopoietic and nonlymphohematopoietic cells, we examined the structure of p24 expressed on different cell types. Tr...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90007-0
更新日期：1985-10-01 00:00:00
abstract：:Large quantities of antibodies are transported into the yolk of the chicken's egg. We have identified several regions within the antibody molecule important for its uptake into the egg yolk. An intact Fc and hinge region but not the Fc-associated carbohydrate are required for transport. Our data suggest that the C(H)2...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(01)00095-5
更新日期：2002-01-01 00:00:00
abstract：:The regulatory role of MEF2 (myocyte enhancer binding factor 2) proteins in nonmuscle tissues has not been well characterized. We examined the expression of MEF2 family members, namely, MEF2A, -B, -C, and -D, in the differentiation of HL60 promyeloid cells and observed the remarkable increase in the expressions of MEF...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(99)00140-6
更新日期：1999-12-01 00:00:00
abstract：:The concomitant effects of trans-chromosomal allotype suppression of both an a VH and an n Cmu locus allotype in multiheterozygous rabbits were investigated. For example of the expression of the a2 VH and n81 Cmu allotypes were suppressed in a multiheterozygous rabbit having the a1 chi-y-n81de12,15f73g74/a2 chi 32y33n...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90257-7
更新日期：1982-01-01 00:00:00
abstract：:The variables regions of three syngeneic anti-idiotypic antibodies (Ab2s) were cloned and sequenced. They are encoded by different VL genes, two are from different members of V kappa-Ox1 superfamily. The H chains are encoded by VH genes belonging to three different VH families, J558, Q52 and 7183. Together with a prev...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90045-y
更新日期：1992-09-01 00:00:00
abstract：:The monocyte chemoattractant protein-1 (MCP-1) functions to recruit monocytes and macrophages to areas of inflammation and is a prototypic chemokine subjected to coordinate regulation by immunomodulatory agents. TNF mediated regulation of MCP-1 occurs through a distal regulatory region located 2.5 kb upstream of the t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(00)00097-3
更新日期：2000-08-01 00:00:00
abstract：:The architecture of lymphoid microenvironments depends upon complex interactions between several stromal cell types. We describe in this report the cloning of a cDNA which encodes a novel membrane molecule containing two external Ig-like domains. It is expressed at the junction between endothelial cells including HEV....
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(98)00102-3
更新日期：1998-12-01 00:00:00
abstract：:Activation of Jurkat T cells with phytohemagglutinin, CD3 or CD2 mAbs results in a marked inhibition of phosphatidylserine (PS) synthesis. Monitoring PS synthesis in T cells shows that: (i) after modulation of CD3 molecules the cells become refractory to further treatment with CD3 mAbs as well as to a further challeng...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90072-2
更新日期：1989-11-01 00:00:00
abstract：:Toll-like receptors (TLRs) are a family of highly conserved germline-encoded pattern-recognition receptors (PRRs) and are essential for host immune response. Little is known regarding the activation mechanism of TLRs especially of the TLR7/8/9 subfamily. Here we cloned and characterized bovine TLR8 (bTLR8) and found t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.09.024
更新日期：2009-02-01 00:00:00
abstract：:Macrophages (Mφ) undergo activation to pro-inflammatory (M1) or anti-inflammatory (M2) phenotypes in response to pathophysiologic stimuli and dysregulation of the M1-M2 balance is often associated with diseases. Therefore, studying mechanisms of macrophage polarization may reveal new drug targets. Human Mφ polarizatio...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.05.027
更新日期：2017-08-01 00:00:00
abstract：:Cattle antibodies have unusually long CDR3 loops in their heavy chains (HCs), and limited light chain (LC) diversity, raising the question of whether these mask the effect of LC variation on antigen recognition. We have investigated the role of the LC in the structure and activity of two neutralizing cattle antibodies...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.04.026
更新日期：2019-08-01 00:00:00
abstract：:In two recent articles published in Molecular Immunology, Steere and colleagues continue their search for an autoimmune mechanism of arthritis in patients who have failed short-course antibiotic therapy for Borrelia burgdorferi, the spirochetal agent of Lyme disease. As in previous attempts, the authors fail to elucid...
journal_title：Molecular immunology
pub_type： 评论,信件
doi：10.1016/j.molimm.2008.02.015
更新日期：2008-06-01 00:00:00
abstract：:CD8+ cytolytic T lymphocytes are essential players of anti-tumor immune responses. On tumors, they recognize peptides of about 8-to-10 amino acids that generally result from the degradation of cellular proteins by the proteasome. Until a decade ago, these peptides were thought to solely correspond to linear fragments ...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2018.03.030
更新日期：2019-09-01 00:00:00
abstract：:Recent studies suggest that CD8(+) T cells with down-regulated CD8 expression (CD3(+)CD8(low) T cells) represented as a distinct phenotype of CD8(+) T cells are increased and linked to disease severity in some chronically persistent infection, such as chronic HIV and parasite infection. However, the role of CD3(+)CD8(...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2013.07.003
更新日期：2013-12-01 00:00:00
abstract：:Nanomaterials are beginning to play an important role in the next generation of immunological assays and biosensors, with potential impacts both in research and clinical practice. In this brief review, we highlight two areas in which nanomaterials are already making new and important contributions in the past 5-10 yea...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2017.12.017
更新日期：2018-06-01 00:00:00
abstract：:Fibroblast-like synoviocyte (FLS) is the ultimate effectual cells in the pathogenesis of rheumatoid arthritis (RA). The current study was undertaken to investigate whether a proliferation-inducing ligand (APRIL) mediates the function of FLS in an animal model of RA. Rat adjuvant-induced arthritis (AA) was induced by i...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.11.002
更新日期：2015-03-01 00:00:00
abstract：:SCID can be caused by various genetic mutations leading to distinctive phenotypes according to the presence of T, B and NK cells. Artemis is a gene encoded on chromosome 10p. The deficiency of this molecule causes an inability to repair DNA double strand breaks and is one of the causes of radiosensitive T-B-NK+ SCID. ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2013.05.004
更新日期：2013-12-01 00:00:00
abstract：:One of the major obstacles in the successful clinical application of monoclonal antibodies has been the development of host immune responses to murine Ig constant and variable regions. While the CDR grafting of MAbs may alleviate many of these problems, the potential remains that one or more murine CDRs on the human I...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(99)00094-2
更新日期：1999-10-01 00:00:00
abstract：:Physicochemical techniques were used to verify previous immunochemical studies showing homology of the human Thy-1 (formerly p25) antigen and the murine Thy-1 or theta antigen. Peptide maps and amino acid compositions showed close similarity between these two proteins; however, they were not identical. These data conf...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90216-4
更新日期：1982-03-01 00:00:00
abstract：:The complete cDNA sequences of two bovine IgGs were obtained by RT-PCR cloning. The first-strand cDNA was prepared from an animal homozygous (A2/A2) for IgG2a; the resulting sequences of the two bovine IgGs reported here were identified as IgG2a(A2) and IgG1. These sequences, and their deduced amino acid sequences, ar...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00107-7
更新日期：1996-02-01 00:00:00
abstract：:Serotonin release from rat basophilic leukemia (RBL) cells, sensitized with a DNP-binding monoclonal IgE, was stimulated with solid surface (polystyrene)-bound DNP-amino acids. The stimulatory potency of DNP-amino acids was dependent on the structure of amino acid attached to DNP. Generally, DNP-amino acids with high ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90134-6
更新日期：1991-06-01 00:00:00
abstract：:Infection of ruminants and humans with Fasciola gigantica is attracting increasing attention due to its economic impact and public health significance. However, little is known of innate immune responses during F. gigantica infection. Here, we investigated the expression profiles of genes involved in Toll-like recepto...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.12.007
更新日期：2018-02-01 00:00:00
abstract：:A panel of hybridomas producing antibodies specific for human growth hormone (hGH) were prepared by using a recombinant hGH [methionylsomatotropin (r-hGH)] as an immunogen. Thirteen representative monoclonal antibodies which showed different reactivity patterns were used to analyze the antigenicities of four different...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90099-4
更新日期：1986-06-01 00:00:00
abstract：:Interleukin (IL)-23 is a heterodimeric cytokine composed of the IL-23-specific subunit p19 and the p40 subunit which also constitutes part of IL-12. IL-23 propagates development of Th17 cells, a novel T cell subset which produces IL-17 but no interferon-gamma or IL-4. For both, IL-23 and IL-23-driven IL-17, a crucial ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.12.008
更新日期：2010-03-01 00:00:00
abstract：:The genetic complexity of the H-2D region includes haplotype disparities in apparent gene and product number. To probe the genetic basis of this complexity, the products of two independently derived mouse strains (STU and B10.SAA48) that express Dw3 antigens were compared. Serologic, fluorometric and peptide map compa...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90007-6
更新日期：1986-10-01 00:00:00
abstract：OBJECTIVE:Spinal cord injury (SCI) is a neurological disease with high incidence and disability. In this paper, we made a foundational study in long non-coding RNA paternally expressed gene 10 (lncRNA PEG10) at PC-12 cells. METHODS:We used CCK8, flow cytometry, migration and invasion assay to detect changes at the cel...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.11.012
更新日期：2020-02-01 00:00:00
abstract：:Properly regulated mitogen-activated protein (MAP) kinase activity is critical for normal thymocyte development. MAP kinases are activated by phosphorylation of tyrosine and threonine, and dual specificity phosphatases (DUSPs) can inactivate MAP kinases by dephosphorylating both tyrosine and threonine. However, a role...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.03.006
更新日期：2006-02-01 00:00:00
abstract：:Forty-eight group A streptococcal strains of different M types were screened for binding of human radiolabeled IgG. Three of the strains bound more than 80% of the added radioactivity and one of them, an M protein type 1 strain designated AP1, was selected for further analysis. Attempts were made to solubilize the IgG...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90071-7
更新日期：1990-06-01 00:00:00
abstract：:Notexin and nigexine are monomeric phospholipases A2(PLA2s) from the venoms of Notechis scutatus scutatus and Naja nigricollis, respectively. Polyclonal antibodies raised in mice against these antigenic proteins displayed non-reciprocal cross-reactivity; anti-notexin antibodies recognized notexin but not nigexine, whe...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90055-5
更新日期：1990-01-01 00:00:00
abstract：BACKGROUND:Individuals genetically deficient of properdin are more susceptible to meningococcal disease. Likewise low concentration or decreased biological activity of mannose-binding lectin (MBL) is associated with higher incidence of bacterial infections during childhood. In this study we report our findings in a Dan...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.02.017
更新日期：2006-02-01 00:00:00
