Abstract：
:Forty-eight group A streptococcal strains of different M types were screened for binding of human radiolabeled IgG. Three of the strains bound more than 80% of the added radioactivity and one of them, an M protein type 1 strain designated AP1, was selected for further analysis. Attempts were made to solubilize the IgG binding bacterial molecule, and small amounts of an IgG binding protein with a mol. wt of 40 kDa could be solubilized with mutanolysin, a muramolytic agent. The gene encoding this streptococcal protein was cloned and expressed in E. coli, and the E. coli-produced protein was purified in a single step by affinity chromatography on IgG-Sepharose. When tested with IgGs from different species, the molecule was found to bind human IgG almost exclusively. The N-terminal amino acid sequence was determined and showed no homology with previously isolated Ig binding proteins, and the name protein H (as in human IgG) is suggested for this novel Ig binding bacterial protein. Protein H showed preferential affinity for heavy chains and Fc fragments of human IgG, and did not bind Ig light chains. The affinity constant, determined by Scatchard plots, between protein H and human polyclonal IgG was 1.6 x 10(9). No binding was observed between protein H and IgM, IgA, IgD, or IgE. Finally, when tested against several additional proteins and human plasma, protein H only showed weak binding to alpha 2-macroglobulin, a proteinase inhibitor.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Akesson P,Cooney J,Kishimoto F,Björck Ldoi
10.1016/0161-5890(90)90071-7subject
Has Abstractpub_date
1990-06-01 00:00:00pages
523-31issue
6eissn
0161-5890issn
1872-9142journal_volume
27pub_type
杂志文章abstract：:In immune cells, Src-family kinases have been implicated as critical regulators of a large number of intracellular signaling pathways. Studies of gene knockout mice lacking various family members have both confirmed the requirement for these kinases in some pathways and provided surprising insight into their roles in ...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2004.04.010
更新日期：2004-07-01 00:00:00
abstract：:B cells play a critical role in immune responses both in physiological and pathological conditions, and microRNAs have been shown to play important roles in regulating B cell proliferation and function. MiR-146a has been shown to modulate T cell immunity, but its function in regulating B cell response remains partiall...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.10.023
更新日期：2017-12-01 00:00:00
abstract：:The C-terminal domain of protein 4.1G was identified to interact with the cytosolic tail of the high affinity IgG receptor, Fc gamma RI, in yeast two-hybrid screens. Proteins of the 4.1 family have previously been found to mediate receptor/cytoskeleton interactions. In the study presented here, we show an alternativel...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.10.024
更新日期：2008-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.01.031
更新日期：2008-05-01 00:00:00
abstract：:In a previous study we isolated a series of rat monoclonal antibodies to the human leukocyte common (LC) antigen and demonstrated that synergistic complement lysis was possible between IgG2b antibodies which recognised different epitopes. In this report we have examined the mechanisms that were involved in synergistic...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90039-3
更新日期：1987-06-01 00:00:00
abstract：:the SC5b-8 complex of human complement is a hydrophilic molecule of mol. wt 800,000-850,000 that is seen in the electron microscope as an elongated, straight or curved structure of 50-55 nm total length and 8-9 nm width. Tryptic attack on the fluid-phase complex exposes lipid-binding surfaces on the molecule. The tryp...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90327-3
更新日期：1982-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.02.024
更新日期：2007-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.04.019
更新日期：2011-07-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(97)00109-0
更新日期：1997-08-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(96)00034-x
更新日期：1996-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.01.029
更新日期：2007-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.09.034
更新日期：2009-02-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.02.110
更新日期：2012-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90038-x
更新日期：1983-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2015.03.011
更新日期：2015-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90086-f
更新日期：1990-08-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.12.005
更新日期：2017-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.04.015
更新日期：2008-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90118-0
更新日期：1987-12-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90032-s
更新日期：1992-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.05.006
更新日期：2014-12-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00030-i
更新日期：1995-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.02.017
更新日期：2010-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90178-m
更新日期：1991-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90145-1
更新日期：1983-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.03.021
更新日期：2020-04-16 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90160-0
更新日期：1994-12-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2013-12-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.04.012
更新日期：2008-07-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.11.004
更新日期：2009-03-01 00:00:00
