Abstract：
:In a previous study we isolated a series of rat monoclonal antibodies to the human leukocyte common (LC) antigen and demonstrated that synergistic complement lysis was possible between IgG2b antibodies which recognised different epitopes. In this report we have examined the mechanisms that were involved in synergistic lysis. We found that the number of C1q binding sites increased from 30,000 to 40,000/cell using a single antibody to 90,000/cell when two IgG2b antibodies to different epitopes were used together. The affinity of C1q binding also increased approx. 3-fold for the synergistic pair, and there was a similar increase in the rate of C1 activation. Combinations of an IgG2b with IgG1 or IgG2a gave much smaller increases in the amount of C1q bound and in the rate of C1 activation. Despite the large number of C1q molecules bound with the optimal synergistic pair and the increased rate of C1 activation, lysis was inefficient in serum depleted of Factor D, suggesting a requirement for the alternative pathway. This is the first demonstration of the need for the alternative pathway in complement lysis by monoclonal antibodies.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Bindon CI,Hale G,Hughes-Jones N,Gorick B,Waldmann Hdoi
10.1016/0161-5890(87)90039-3subject
Has Abstractpub_date
1987-06-01 00:00:00pages
587-94issue
6eissn
0161-5890issn
1872-9142journal_volume
24pub_type
杂志文章abstract：:The RING finger type E3 ubiquitin ligase, Cbl-b, is abundantly expressed in bone marrow-derived mast cells (BMMCs) and functions as a potent negative regulator of signalling responses from the high-affinity IgE receptor (FcvarepsilonRI). To determine the contribution of Cbl-b E3 ligase activity we generated knockin mi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.08.002
更新日期：2008-02-01 00:00:00
abstract：:The high affinity receptor for IgE, which mediates allergic reactions, is found exclusively on mast cells, basophils and epidermal Langerhans cells. It is composed of an alpha, beta and two gamma-polypeptide chains, inserted as a complex into the plasma membrane. The alpha and beta-subunits are produced only by these ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(96)84614-1
更新日期：1996-07-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90110-l
更新日期：1990-02-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.08.001
更新日期：2007-02-01 00:00:00
abstract：OBJECTIVE:To investigate the impact of death-associated protein kinase 1 (Dapk1) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) via p38MAPK/NF-κB pathway. METHODS:Dapk1+/+ and Dapk1-/- mice were randomized into Control, LPS, SB203580 (a p38MAPK pathway inhibitor) + LPS, and PDTC (a NF-κB pathway inhibitor...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.01.014
更新日期：2020-04-01 00:00:00
abstract：:The objective of this study was to characterize the in vitro and in vivo activity of a novel afucosylated rituximab (BLX-300) expressed in a Lemna aquatic plant-based system free of zoonotic pathogens. The glycosylation of BLX-300 was shown to be homogeneous, composed of a single major N-glycan species without detecta...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.01.001
更新日期：2012-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.05.007
更新日期：2012-10-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.09.016
更新日期：2018-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2004.04.010
更新日期：2004-07-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.12.005
更新日期：2010-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.05.019
更新日期：2009-08-01 00:00:00
abstract：:The cDNA coding for a hybridoma anti Shigella dysenteriae type 1 antibody (3707 E9) has been cloned, and sequenced. Binding patterns with fragments of the bacterial polysaccharide antigen had already been studied in detail. The VH sequence utilizes the VH441 gene, first identified amongst beta-(1,6)galactan-binding an...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00072-m
更新日期：1995-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.04.028
更新日期：2004-07-01 00:00:00
abstract：:A panel of human T cell clones specific for measles virus was characterized and among them fusion protein-specific, DR1-and DP-restricted T cell clones were selected to study the processing and presentation of determinants borne by a viral membrane protein. Using two independent methods to assess the activation of T c...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00148-4
更新日期：1996-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90164-1
更新日期：1986-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2004.03.012
更新日期：2004-06-01 00:00:00
abstract：:The B1 molecule (CD20) is a phosphoprotein expressed only by B-lymphocytes. In this study, analysis of B1 immunoprecipitated from surface iodinated B-cell lines and B-lymphocytes has shown that there are several expressed forms of B1. A predominant species of Mr 33,000 represents 75-80% of the iodinated cell surface B...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90047-8
更新日期：1988-12-01 00:00:00
abstract：:Purified mRNA's encoding the HLA-A and -B antigen heavy chains or beta 2-microglobulin were prepared from human B lymphoid cells by positive hybridization selection procedures. The role of chain association in the biosynthesis and intracellular transport of HLA-A and -B antigens was investigated by injecting these mRN...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90115-x
更新日期：1986-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2013.10.005
更新日期：2014-02-01 00:00:00
abstract：:The complement system is very tightly regulated by fluid-phase and membrane-bound factors that prevent injury to self-tissues. The study of genetically engineered animals with targeted deletion or gain of function mutations has highlighted the important role that many of the complement inhibitors play in vivo. The adv...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/s0161-5890(03)00110-x
更新日期：2003-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(84)90023-3
更新日期：1984-08-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.07.025
更新日期：2019-10-01 00:00:00
abstract：:Antibacterial responses have been studied in Atlantic salmon following an acute intra peritoneal injection of a genetically attenuated (aroA(-)) strain of Aeromonas salmonicida known to elicit protective immunity. Three tissues were studied for transcriptional changes, the liver, head kidney and the gill. RNA was coll...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.10.007
更新日期：2006-04-01 00:00:00
abstract：:Candida albicans is an opportunistic human-pathogenic fungus, which can cause superficial but also life-threatening invasive infections. The pH-regulated antigen 1 (Pra1) of C. albicans is a surface-associated and secreted protein highly expressed in the hyphal form. Pra1 can bind to complement receptor 3 (CD11b/CD18)...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.07.007
更新日期：2011-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.09.004
更新日期：2016-10-01 00:00:00
abstract：:T-lymphocyte proliferative responses were determined with peripheral blood lymphocytes (PBL), peritoneal exudate lymphocytes (PEL) and lymph node lymphocytes (LNL) of guinea pigs immunized with horse cytochrome c (HCytc). The proliferative response of PBL activated by peptide 81-104 from animals immunized with HCytc w...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90115-0
更新日期：1988-08-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2013.06.016
更新日期：2013-12-01 00:00:00
abstract：:Most of the complement proteins in circulation are, by and large, synthesized in the liver. However data accumulated over the past several decades provide incontrovertible evidence that some if not most of the individual complement proteins are also synthesized extrahepatically by activated as well as non-activated ce...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2016.11.003
更新日期：2017-04-01 00:00:00
abstract：:An important function of Fcγ receptors is the removal of IgG-containing immune complexes from the circulation. The activating receptor FcγRIIA and inhibitory receptor FcγRIIB2 are both expressed on human myeloid cells, and are both capable of mediating endocytosis of immune complexes. We studied endocytosis of these t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.09.003
更新日期：2011-10-01 00:00:00
abstract：:Recognition of double-stranded RNA (dsRNA) activates interferon-regulatory factor 3 (IRF3)-dependent expression of anti-viral factors. The innate immune system recognizes viral dsRNA through two distinct pathways. First, the Toll-like receptor 3 (TLR3) detects dsRNA phagocytosed in endosomes. In addition, the helicase...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.03.012
更新日期：2008-06-01 00:00:00
