Abstract：
:Purified mRNA's encoding the HLA-A and -B antigen heavy chains or beta 2-microglobulin were prepared from human B lymphoid cells by positive hybridization selection procedures. The role of chain association in the biosynthesis and intracellular transport of HLA-A and -B antigens was investigated by injecting these mRNA species into Xenopus laevis oocytes and following the fates of the translated products by immunoprecipitation. When mRNA encoding beta 2-microglobulin from the B lymphoblastoid cell line MST was coinjected with mRNA encoding the HLA-A and -B antigen heavy chains from the Burkitt lymphoma cell line Daudi, fully assembled class I antigens were detected using the monoclonal antibody W6/32. This result suggested that there may be no defect in the mRNA encoding Daudi HLA-A and -B antigen heavy chains. When the state of maturity of the N-linked glycan units on these class I antigen heavy chains was assessed, they were found to have undergone some processing. In contrast, when mRNA encoding immunoglobulin M (IgM) was injected into oocytes, the glycan units of the IgM heavy chains were found to be in the unprocessed (high mannose) form. This result shows that Xenopus oocytes can process some eukaryotic glycoproteins of exogenous origin.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Kinnon C,Owen MJdoi
10.1016/0161-5890(86)90115-xsubject
Has Abstractpub_date
1986-05-01 00:00:00pages
525-32issue
5eissn
0161-5890issn
1872-9142journal_volume
23pub_type
杂志文章abstract：:A sheep cDNA library was screened with a human C mu probe, and the complete nucleotide sequence of a 1923 nt cDNA was determined. It contains sequences corresponding to all the exons (VH, DH, JH, CH1, CH2, CH3 and CH4) characteristic of the immunoglobulin mu heavy chain regions. The deduced amino acid sequence shows a...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90120-m
更新日期：1992-07-01 00:00:00
abstract：:The chemokine receptor CCR2 binds four pro-inflammatory monocyte chemoattractant proteins, designated MCP1/CCL2, MCP2/CCL8, MCP3/CCL7 and MCP4/CCL13. This study demonstrates the important biology of this receptor during the response to the chemokine milieu. Competitive chemotaxis and calcium flux assays were performed...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.09.033
更新日期：2007-03-01 00:00:00
abstract：:Monoclonal antibodies (MAbs) raised against trout immunoglobulin (Ig) recognize two independent Ig populations differing in the molecular weight of L chains. The trout Ig population recognized by MAb 2H9 represented congruent to 20% of the total Ig and contained L chains congruent to 26 kDa, whereas the trout Ig popul...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90014-b
更新日期：1991-11-01 00:00:00
abstract：:We have recently reported cellular growth arrest induced following crosslinking of surface IgM (sIgM) but not surface IgD (sIgD) in the WEHI-231 cell line, representative of the immature B cell stage, and its delta heavy chain (delta) transfectant. An initial report has indicated WEHI-231.7, a subclone of WEHI-231, fa...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90198-7
更新日期：1992-05-01 00:00:00
abstract：:In Drosophila the synthesis of antimicrobial peptides in response to microbial infections is under the control of the Toll and immune deficiency (Imd) signaling pathways. The Toll signaling pathway responds mainly to Gram-positive bacterial and fungal infection while the Imd pathway mediates the response to Gram-negat...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.01.015
更新日期：2008-05-01 00:00:00
abstract：:Monoclonal antibodies to cyclosporine A (Cs), a potent immunosuppressant, were generated in BALB/c mice using a novel antigen prepared by linking Cs to a protein carrier via a photoactive cross-linking reagent, 4-benzoylbenzoic acid (BBa). Twenty-two monoclonal anti-Cs antibodies were generated, using Cs-BBa-bovine se...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90162-q
更新日期：1992-01-01 00:00:00
abstract：BACKGROUND:Dendritic cells (DCs) and regulatory T (Treg) cells are crucial for inducing immune tolerance. However, the suppressive function of infused Treg cells and immature DCs (imDCs) following solid organ transplantation remains unclear. METHODS:ImDCs derived from DA-donor rats and Treg cells isolated from spleens...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.10.004
更新日期：2016-11-01 00:00:00
abstract：:alpha 2-Macroglobulin (alpha 2M) complexed with proteinases or modified by the action of amines has been shown to affect immune responses in vitro, though as yet the mechanisms are poorly understood. Supernates from rabbit lymphoid cells cultured in medium with normal rabbit serum and 35S-methionine (or 14C-leucine) w...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90079-5
更新日期：1989-03-01 00:00:00
abstract：:Antibodies (Ab) are bifunctional molecules with two domains, a constant region (C) that confers effector properties and a variable (V) region responsible of antigen (Ag) binding. Historically the C and V regions were considered to be functionally independent, with Ag specificity being solely determined by the V region...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.02.018
更新日期：2010-04-01 00:00:00
abstract：:The structural and functional integrity of the cell is largely maintained by protein-protein interactions. Recently, we demonstrated that multiple antigenic peptides (MAPs) constructed from 60 kDa Ro sequence could be used to show intramolecular and intermolecular protein-protein interaction within the 60 kDa Ro ribon...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.03.036
更新日期：2004-07-01 00:00:00
abstract：:A Brucella abortus protein with a molecular weight of 50 kDa has been shown to bind bovine immunoglobulin G from healthy, brucellosis-free animals. The Brucella immunoglobulin G binding molecule appears to be a protein, since it is susceptible to proteolysis. The protein is presumed to be located on the cell surface, ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90084-w
更新日期：1991-01-01 00:00:00
abstract：:We have examined the properties of soluble guanylate cyclase activity in the human neutrophil. The enzyme showed complex regulation by metal ions. A 10-fold higher activity was observed in the presence of Mn2+ than Mg2+, while Ca2+ caused an increase in activity only in the presence of Mg2+ ion. Sodium nitroprusside (...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90138-5
更新日期：1985-07-01 00:00:00
abstract：:The number of V alpha and V beta sequences of T cell receptors now available allows a meaningful analysis of their variability profiles. Variability plots were derived using a modified form of Wu and Kabat's algorithm: variability is not computed as a proportion of the number of different residues occurring at a posit...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90178-m
更新日期：1991-09-01 00:00:00
abstract：:T regulatory (Treg)/T-helper (Th) 17 imbalance has been shown to integrate with epigenetics to result in the development of autoimmune diseases. We aim to investigate the influence of disease staging on Treg/Th17 cells and whether the aberrant DNA methylation is implicated in the development of rheumatoid arthritis (R...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.08.002
更新日期：2020-11-01 00:00:00
abstract：:As demonstrated by others, diisopropyl fluorophosphate (DFP) markedly inhibits the O2- generation from guinea-pig polymorphonuclear leukocytes (PMN) stimulated by an antibody complex with ovalbumin (Ag-Ab complex), and also the intracellular uptake of antibody-sensitized erythrocytes by the cells. However, when PMN we...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90113-0
更新日期：1985-09-01 00:00:00
abstract：:Intercellular adhesion molecule-1 (ICAM-1) functions in leukocyte trafficking, activation, and the formation of the immunological synapse. ICAM-1 is a member of the immunoglobulin superfamily of adhesion proteins, which share a similar structure of repeating Ig-like domains. Many genes in this family, including ICAM-1...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.03.005
更新日期：2010-05-01 00:00:00
abstract：:Polyclonal or monoclonal human IgM rheumatoid factors (RF) react with eight antigenic sites on the CH3 IgG domain, four sites on CH2 and two on human beta 2-microglobulin. All 14 of these RF-reactive epitopes are linear 7-11 amino acid peptides with different primary sequence. We questioned whether RF reactivity with ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(97)00024-2
更新日期：1997-05-01 00:00:00
abstract：:SHP-1 plays an important role for the regulation of signaling from various hematopoietic cell receptors. In this study, we examined IL-3-induced cell proliferation and IL-3 depletion-induced apoptosis in bone marrow-derived mast cells (BMMC) established from motheaten (me) that lack SHP-1 expression, viable motheaten ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.10.001
更新日期：2011-01-01 00:00:00
abstract：:Tunicamycin inhibited the synthesis of glycosylated mu-chains and kappa-chains in the human lymphoblastoid cell line, Daudi. Nonglycosylated IgM could not be detected on the surface of tunicamycin-treated cells by cell surface iodination techniques even under conditions where membrane IgM was re-expressed in control c...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90106-2
更新日期：1983-01-01 00:00:00
abstract：:The Class II Transactivator (CIITA) is the master regulator of Major Histocompatibility Class II (MHC II) genes. Transcription of CIITA through the IFN-γ inducible CIITA promoter IV (CIITA pIV) during activation is characterized by a decrease in trimethylation of histone H3 lysine 27 (H3K27me3), catalyzed by the histo...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2015.08.003
更新日期：2015-10-01 00:00:00
abstract：:Major histocompatibility complex (MHC) class II molecules are cell surface glycoproteins and are known to display processed antigens on the surface of antigen presenting cells (APC). Within the APC, the loading of processed antigenic peptides to MHC class II molecules is known to take place in the endosomal compartmen...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00030-i
更新日期：1995-06-01 00:00:00
abstract：:Calcium signals are crucial for diverse cellular functions including adhesion, differentiation, proliferation, effector functions and gene expression. After engagement of the B cell receptor, the intracellular calcium ion (Ca(2+)) concentration is increased promoting the activation of various signaling cascades. While...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2013.10.006
更新日期：2014-12-01 00:00:00
abstract：:Macrophage migration is mediated by complex cellular signaling processes and cytoskeleton re-arrangement. In particular, recent advances indicate that the innate immunity signaling process plays a key role in the regulation of macrophage migration. In this report, we have provided evidence demonstrating the involvemen...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.12.004
更新日期：2010-03-01 00:00:00
abstract：:Humanization of rodent mAbs by CDR-grafting (also called "reshaping") is now a standard procedure for reducing immunogenicity and recruiting human effector functions. However, the design of the humanized mAb can sometimes prove circuitous. Attempts were made to humanize L-25, a mouse antibody against the human alpha-4...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(99)00087-5
更新日期：1999-08-01 00:00:00
abstract：:IFN-gamma plays an essential role in the IL-12/IL-23/IFN-gamma pathway that is required for the defense against intracellular pathogens. In the IFN-gammaR1 several amino acid substitutions have been reported that abrogate IFN-gamma signaling. These substitutions can lead to a null phenotype and enhanced susceptibility...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.11.016
更新日期：2010-02-01 00:00:00
abstract：:Coinfection with leishmaniasis and schistosomiasis has been associated with increased time to healing of cutaneous lesions of leishmaniasis. The objective of this study was to evaluate the effect of Leishmania braziliensis infection on co-cultures of monocyte-derived dendritic cells (MoDCs) with autologous lymphocytes...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.11.018
更新日期：2018-01-01 00:00:00
abstract：:Lysozyme is an important component of the insect non-specific immune response against bacteria that is characterized by its ability to break down bacterial cell-walls. By searching an EST database from the fall armyworm, Spodoptera frugiperda (Negre et al., 2006), we identified five sequences encoding proteins of the ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.09.028
更新日期：2009-12-01 00:00:00
abstract：:A transplanted organ suffers inherently from an ischaemic insult and subsequent reperfusion injury. The severity of such early events is thought to influence the success of the transplant procedure, not only in the immediate post-transplant period, but also to predispose the graft to both acute and chronic rejection. ...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/s0161-5890(99)00118-2
更新日期：1999-09-01 00:00:00
abstract：:The architecture of lymphoid microenvironments depends upon complex interactions between several stromal cell types. We describe in this report the cloning of a cDNA which encodes a novel membrane molecule containing two external Ig-like domains. It is expressed at the junction between endothelial cells including HEV....
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(98)00102-3
更新日期：1998-12-01 00:00:00
abstract：:During the last years, intensive research has shed light in the transcriptional networks that shape the invariant NKT (iNKT) cell lineage and guide the choices towards functionally distinct iNKT cell subsets (Constantinides and Bendelac, 2013; Engel and Kronenberg, 2014; Gapin, 2016; Kim et al., 2015). However, the ep...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2018.08.020
更新日期：2018-09-01 00:00:00
