Abstract:
:The chemokine receptor CCR2 binds four pro-inflammatory monocyte chemoattractant proteins, designated MCP1/CCL2, MCP2/CCL8, MCP3/CCL7 and MCP4/CCL13. This study demonstrates the important biology of this receptor during the response to the chemokine milieu. Competitive chemotaxis and calcium flux assays were performed utilising mixtures of chemokines to assess a hierarchal arrangement of chemokine prepotency; these demonstrated that the MCP2-CCR2 interaction is able to supersede signals generated by RANTES, another pro-inflammatory chemokine, or the homeostatic chemokine SDF1. These observations were validated using three physiologically relevant monocytic cell lines. Having identified the importance of CCR2, experiments were then performed to examine the signal transduction processes coupled to this receptor. G protein coupling was initially examined; Cholera toxin reduced the chemotactic response to MCP2 (p<0.001), whilst the response to the other MCP chemokines remained normal. The response to MCP2 was uniquely inhibited by elevated concentrations of cAMP and, unlike MCP1, 3 and 4 (p<0.05), MCP2 failed to inhibit adenylate cyclase. Expression of dominant negative H-ras demonstrated that each MCP chemokine required active ras in order to elicit ERK activation and a chemotactic response. Unlike MCP1, MCP2 failed to induce nuclear translocation of activated ERK1 or subsequent induction of c-Myc expression. Akt activation also showed ligand-specific differences, with MCP2 producing a delayed response compared to the other MCP chemokines. Together these data highlight the importance of CCR2 and suggest that it is a powerful tool for fine tuning the immune response.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
O'Boyle G,Brain JG,Kirby JA,Ali Sdoi
10.1016/j.molimm.2006.09.033subject
Has Abstractpub_date
2007-03-01 00:00:00pages
1944-53issue
8eissn
0161-5890issn
1872-9142pii
S0161-5890(06)00640-7journal_volume
44pub_type
杂志文章abstract::Tumor-associated macrophages (TAMs) are preferentially M2 skewed and promote tumor growth, angiogenesis, invasion, and/or metastasis. In this study, we have analyzed the in vitro immunomodulatory potential of a non-toxic neem leaf glycoprotein (NLGP) in reprogramming Stage III supraglottic laryngeal tumor cell lysate ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2014.01.015
更新日期:2014-06-01 00:00:00
abstract::A sheep cDNA library was screened with a human C mu probe, and the complete nucleotide sequence of a 1923 nt cDNA was determined. It contains sequences corresponding to all the exons (VH, DH, JH, CH1, CH2, CH3 and CH4) characteristic of the immunoglobulin mu heavy chain regions. The deduced amino acid sequence shows a...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(92)90120-m
更新日期:1992-07-01 00:00:00
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更新日期:1992-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2018.05.009
更新日期:2018-07-01 00:00:00
abstract::Individually amplified kappa cDNA molecules from single B lymphocytes revealed sequence heterogeneity and aberrantly spliced products. The nature and frequency of the base changes and their absence from similarly amplified beta2 microglobulin transcripts indicate that they were not derived by Taq polymerase misincorpo...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(00)00055-9
更新日期:2000-04-01 00:00:00
abstract:BACKGROUND:Atopic dermatitis (AD) or atopic eczema is a skin disease characterized with itching, increased serum level of immunoglobulin E, and peripheral eosinophilia. Thymoquinone (TQ) is an important ingredient of Nigella sativa seeds having antioxidant and anti-inflammatory effects. OBJECTIVE:Present study investi...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2018.07.013
更新日期:2018-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2014.02.002
更新日期:2014-06-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90069-n
更新日期:1993-03-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1016/s0161-5890(00)00018-3
更新日期:2000-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.molimm.2004.03.001
更新日期:2004-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90187-8
更新日期:1987-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(92)90168-w
更新日期:1992-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.09.009
更新日期:2009-12-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(88)90061-2
更新日期:1988-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/0161-5890(92)90001-e
更新日期:1992-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2017.05.028
更新日期:2017-09-01 00:00:00
abstract::Lysozyme is an important component of the insect non-specific immune response against bacteria that is characterized by its ability to break down bacterial cell-walls. By searching an EST database from the fall armyworm, Spodoptera frugiperda (Negre et al., 2006), we identified five sequences encoding proteins of the ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.09.028
更新日期:2009-12-01 00:00:00
abstract::PKZ, protein kinase containing Z-DNA binding domains, is a novel member of eIF2α kinase in fish. PKZ can be up-regulated in response to Poly I:C or heat stress, and it has a typical eIF2α kinase activity. However, the relationship between fish PKZ and apoptosis remains unclear. In the present study, effects of PKZ on ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2015.11.006
更新日期:2016-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1982-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1993-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2007-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.05.026
更新日期:2008-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90158-1
更新日期:1987-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1990-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90047-f
更新日期:1993-10-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.06.013
更新日期:2016-08-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.11.006
更新日期:2010-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:1994-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1982-02-01 00:00:00