Abstract:
:Coinfection with leishmaniasis and schistosomiasis has been associated with increased time to healing of cutaneous lesions of leishmaniasis. The objective of this study was to evaluate the effect of Leishmania braziliensis infection on co-cultures of monocyte-derived dendritic cells (MoDCs) with autologous lymphocytes from patients with schistosomiasis and patients with cutaneous leishmaniasis. MoDCs were differentiated from peripheral blood monocytes, isolated by magnetic beads, infected with L. braziliensis, and co-cultured with autologous lymphocytes. Expression of HLA-DR, CD1a, CD83, CD80, CD86, CD40, and the IL-10 receptor (IL-10R) on MoDCs as well as CD28, CD40L, CD25, and CTLA-4 on lymphocytes were evaluated by flow cytometry. The production of the cytokines IL-10, TNF, IL-12p40, and IFN-γ were evaluated by sandwich ELISA of the culture supernatant. The infectivity evaluation was performed by light microscopy after concentration of cells by cytospin and Giemsa staining. It was observed that the frequency of MoDCs expressing CD83, CD80, and CD86 as well as the MFI of HLA-DR were smaller in the group of patients with schistosomiasis compared to the group of patients with leishmaniasis. On the other hand, the frequency of IL-10R on MoDCs was higher in patients with schistosomiasis than in patients with leishmaniasis. CD4+ and CD8+ T lymphocytes from patients with schistosomiasis presented a lower frequency of CD28 and a higher frequency of CTLA-4 compared to lymphocytes from patients with leishmaniasis. Levels of IL-10 were higher in the supernatants of co-cultures from individuals with schistosomiasis compared to those with leishmaniasis. However, levels of TNF, IL-12p40, and IFN-γ were lower in the group of individuals with schistosomiasis. Regarding the frequency of MoDCs infected by L. braziliensis after 72h in culture, it was observed that higher frequencies of cells from patients with schistosomiasis were infected compared to cells from patients with leishmaniasis. It was concluded that MoDCs from patients with schistosomiasis are more likely to be infected by L. braziliensis, possibly due to a lower degree of activation and a regulatory profile.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Lopes DM,de Almeida TVVS,de Souza RDP,Ribeiro LEV,Page B,Fernandes JS,Carvalho EM,Cardoso LSdoi
10.1016/j.molimm.2017.11.018subject
Has Abstractpub_date
2018-01-01 00:00:00pages
173-183eissn
0161-5890issn
1872-9142pii
S0161-5890(17)30581-3journal_volume
93pub_type
杂志文章abstract::Recently, basophils have been suggested to produce inflammatory mediators in response to IgE in the absence of allergens. Monocyte chemoattractant protein-1 (MCP-1) plays an important role in the initiation of inflammatory responses by recruiting various immune cells to the site of allergic inflammation. In the presen...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2014.06.008
更新日期:2014-11-01 00:00:00
abstract::The translation of mRNA within total RNA of German (Blattella germanica) cockroaches was performed using a rabbit reticulocyte lysate system. Analysis of the translation products by SDS-PAGE and combined autoradiography revealed several synthesized proteins with apparent molecular weights ranging from 20 kD to 110 kD....
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90443-f
更新日期:1993-12-01 00:00:00
abstract::Armenian hamsters were immunized with non-liganded, partially liganded or affinity-labeled anti-fluorescein Mab 4-4-20. Seventeen hybridoma producing monoclonal anti-4-4-20 antibodies were characterized from chemically-mediated fusions of immune hamster lymphocytes with murine Sp2/O-Ag14 myeloma cells. Distinct popula...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90125-u
更新日期:1993-08-01 00:00:00
abstract::In humans, three distinct but closely related classes of receptors that bind the Fc portion of IgG (FcgammaRI, II and III) have been identified. FcgammaRI can bind monomeric IgG with high affinity and has a unique third extracellular domain (EC3). Three very similar genes have been characterized for FcgammaRI (A, B, C...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(98)00079-0
更新日期:1998-10-01 00:00:00
abstract::The three-dimensional structure of the Mcg lambda-type Bence-Jones dimer crystallized in ammonium sulfate is known at 2.3-A resolution. A series of nine other human lambda-chains and two kappa-chains did not crystallize under the same conditions. After these proteins were hybridized with the Mcg light chain by the met...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(85)80001-8
更新日期:1985-02-01 00:00:00
abstract::Hybridomas producing anti-histone monoclonal antibodies were generated from a 2-month old MRL lpr/lpr mouse. Five IgM antibodies showed binding to histone H3 and three of these IgM antibodies also bound histone H1. Two IgG2a antibodies (MRA3 and MRA12) were specific for histone H1 and their binding was further charact...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(89)90035-7
更新日期:1989-08-01 00:00:00
abstract::A high affinity C5 convertase is generated when a C3 convertase deposits additional C3b molecules on and around itself thereby switching the substrate specificity of C3 convertase from C3 to C5. In the present study the role of the additional C3b molecules in influencing the regulation of classical pathway C5 converta...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.07.282
更新日期:2007-02-01 00:00:00
abstract:BACKGROUND:Complement activation involved in the innate immunity and adaptive immunity and further contributed to the development of tumor growth. This study aimed to investigate the association of genetic variants in complement 3 (C3) and decay-accelerating factor (DAF) genes with the risk of gastric cancer. METHODS:...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2014.10.006
更新日期:2015-02-01 00:00:00
abstract::In a recent article published in Molecular Immunology [Furukawa, K., Shimizu, T., Murakami, A., Kono, R., Nakagawa, M., Sagawa, T., Yamato, I., Azuma, T., 2007. Strategy for affinity maturation of an antibody with high evolvability to (4-hydroxy-3-nitrophenyl) acetyl hapten. Mol. Immunol. 44, 2436-2445], the authors m...
journal_title:Molecular immunology
pub_type: 评论,信件
doi:10.1016/j.molimm.2007.07.007
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:A T cell subtype with the CD4+CD25-FoxP3+ phenotype was recently described. We aimed to investigate the frequency of these cells and their ability to produce cytokines in tumor-draining lymph nodes from patients with breast cancer (BC). MATERIALS AND METHODS:Mononuclear cells from lymph nodes of 20 patients...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.10.007
更新日期:2019-12-01 00:00:00
abstract::Chagas' disease, caused by Trypanosoma cruzi, is an inflammatory disorder leading to chronic Chagas cardiomyopathy (CCC). Only one third of T. cruzi-infected individuals progress to CCC while the others are considered asymptomatic (ASY). The human inhibitory kappaB-like gene (IKBL/NFKBIL1), homologous to the IkappaB f...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.04.015
更新日期:2008-01-01 00:00:00
abstract::Glucocorticoids down regulate the inducible nitric oxide synthase (iNOS) gene both transcriptionally and post-transcriptionally. The post-transcriptional events are suggested to involve destabilization of the iNOS transcript although the molecular mechanisms for this effect are not known. Recently, our laboratory demo...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.01.029
更新日期:2007-05-01 00:00:00
abstract::Linear IgE-binding epitopes identified in legumin allergens of peanut (Ara h 3) and other allergenic tree nuts (Jug r 4 of walnut, Cor a 9 of hazelnut, Ana o 2 cashew nut) were mapped on three-dimensional models of the proteins built up by homology modelling. A conformational analysis revealed that consensual surface-...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.01.023
更新日期:2007-05-01 00:00:00
abstract:UNLABELLED:C5L2, a G-protein-coupled receptor (GPCR), has been identified as an ASP (C3adesArg) and C5a receptor. Controversy exists regarding both ligand binding and functionality. ASP activation of C5L2 is proposed to regulate fat storage. C5L2 is also proposed as a decoy receptor for C5a, an inflammatory mediator, b...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.06.007
更新日期:2009-09-01 00:00:00
abstract::The development and maintenance of thymic microenvironments depends on sustained crosstalk signals derived from developing thymocytes. However, the molecular basis for the initial phase in the lymphoid dependent development of thymic epithelial cells (TECs) remains unclear. Here we show that similarly to regular thymo...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.01.015
更新日期:2009-05-01 00:00:00
abstract::Ovalbumin (OVA) is a major allergen (Gal d II) of hen egg white and is often the cause of hypersensitivity reactions to food. Further knowledge of the antigenic and allergenic epitopes of allergens will provide better treatment of this disease. To analyse these epitopes we produced a panel of monoclonal antibodies (mA...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(92)90055-3
更新日期:1992-10-01 00:00:00
abstract::Mammalian B-cell specific somatic hypermutation contributes to affinity maturation of the antibody response. This mutator activity is highly focused on rearranged immunoglobulin variable regions, but the underlying mechanism remains to be elucidated. In an effort to gain insights into the mechanism of somatic hypermut...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(94)90081-7
更新日期:1994-02-01 00:00:00
abstract::We previously showed that a variety of amino acid substitutions at positions 58 and 59 in the V(H) CDR2 of an anti-arsonate (Ars) antibody Fab simultaneously resulted in increased or unaltered affinity for Ars and substantially enhanced affinity for DNA. To test the generality of these observations, we generated and c...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(98)00005-4
更新日期:1997-12-01 00:00:00
abstract::Specific IgE antibodies have been affinity-purified from recombinant grass pollen allergens, and used to identify isoforms of the two major allergens of rye-grass pollen, Lol p I and Lol p V and cross-reactive allergens in other grasses. Lol p I-specific IgE (affinity-purified from the recombinant protein expressed by...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(94)90068-x
更新日期:1994-04-01 00:00:00
abstract::NKG2D receptor-ligand interaction triggers NK cell-mediated cytolysis and IFN-gamma secretion. IFN-gamma produced by NK cells has been found to promote the interaction between NK cells and monocytes; however, the underlying mechanism remains elusive. We demonstrate here that IFN-gamma exclusively induced or upregulate...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.10.007
更新日期:2008-03-01 00:00:00
abstract::Evidence suggests that immune dysregulation is associated with autism spectrum disorder (ASD). T cell immunoglobulin and mucin domain-3 (TIM-3) has a critical role in several inflammatory disorders; however, the role of TIM-3 signaling has not been demonstrated in ASD. In the present study, we assessed the role of TIM...
journal_title:Molecular immunology
pub_type: 临床试验,杂志文章
doi:10.1016/j.molimm.2018.12.020
更新日期:2019-02-01 00:00:00
abstract::Vitamin D has been recommended as an immune modulator in recent years, in addition to regulating calcium-phosphorous-bone metabolism. Clinical studies on organ transplantation found that vitamin D sufficiency patients were less likely to develop acute cellular rejection within one year after transplantation compared t...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.09.006
更新日期:2017-11-01 00:00:00
abstract::Spondyloarthritis is the second most common form of chronic inflammatory arthritis and a unique hallmark of the disease is pathologic new bone formation. Several cytokine pathways have been genetically associated with ankylosing spondylitis (AS), the prototypic subtype of SpA, and additional evidence from human and an...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2013.07.016
更新日期:2014-01-01 00:00:00
abstract::Previous investigation have demonstrated the presence of the Rho(D) antigen in Rh negative erythrocytes. The intact Rh negative cell, however, does not bind anti-D IgG. Presently we have shown that an anti-D binding antigen resides on the cytoplasmic surface of Rh negative erythrocyte membranes. Unsealed Rh negative m...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(83)90091-3
更新日期:1983-05-01 00:00:00
abstract::In periodontal disease, IgG1 and IgA1 antibodies produced in situ deposit on antigens in the affected tissues. Thus, there is an interest in the effect of co-deposited IgA1 antibodies on complement activation by IgG1-immune complexes. In the present study, we first analyzed the effect of IgA1-immune complexes on compl...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.02.004
更新日期:2006-02-01 00:00:00
abstract::Inhibition of the catalytic activity of horseradish peroxidase by rabbit antisera was measured using alternate enzyme substrates. The same general inhibition curve patterns were obtained regardless of the electron donor or hydroperoxide used. In all cases typical biphasic inhibition patterns were found and complete in...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(83)90169-4
更新日期:1983-12-01 00:00:00
abstract::The human monocyte-like cell line, U-937, was shown to synthesize and secrete C3 by hemolytic assays, radioimmunoassays and metabolic labeling experiments. The daily synthesis of antigenic C3 by unstimulated U-937 cells was low (about 3 ng/10(6) cells/24 hr) over a 3 day period. Induction of the cells to differentiate...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90079-4
更新日期:1987-10-01 00:00:00
abstract::Immunization of different mice strains with a recombinant fusion protein composed of the vector-encoded N-terminal leader peptide CroLac (containing lambda Cro and LacI fragments) and a part of the transmembrane protein of HIV-1 (gp41) led to a high anti-CroLac humoral immune response. A detailed analysis of this resp...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90144-z
更新日期:1993-06-01 00:00:00
abstract::Two monoclonal antibodies, HH8 and HH9, have been established after immunization of mice with galactosyl-A glycolipid antigen having the terminal structure, Gal beta 1----3GalNAc alpha 1----3[Fuc alpha 1----2]Gal beta 1----R, which is the precursor for type 3 chain A (repetitive A) and type 3 chain H (A-associated H)....
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(88)90068-5
更新日期:1988-02-01 00:00:00
abstract::Dendritic cells (DC) are comprised of several subsets with distinct functions, differing in their capacity to respond to pathogen products. To gain novel insights into their pathogen specificity, we compared the protein composition of the plasma membrane of CD8+ and CD8- DC, directly isolated from mouse spleens. Diffe...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.02.028
更新日期:2010-05-01 00:00:00