Abstract：
:A relatively large number of variable region genes (V) contribute, via gene rearrangements with smaller numbers of additional gene elements (D and J), to generate diversity in the immune response. While some VH gene families are thought to contain 100- 1000 members, the VH10 family has only two known functioning members with 99% sequence homology. Both members (monoclonal antibodies) are capable of binding DNA, and since they were derived from inbred mice afflicted with the lupus syndrome they are considered autoimmune antibodies. Relative uniqueness of the VH10 primary nucleotide sequence presents a model system with which to examine unrearranged VH genes and attempt to identify germline genes eventually expressed as autoantibodies. PCR amplified germline sequences of the VH10 family are highly conserved, with few base substitutions evenly distributed between both framework and CDR regions. It was determined that the PCR amplified germline sequences are highly similar to the DNA sequences of the two monoclonal VH10 antibodies, and a non-functional psuedo-germline gene was found that is identical to a non-functional cDNA derived from a hybridoma cell line. These findings indicate that the use of unique CDR DNA sequences for the identification and amplification of specific germline V genes via PCR can yield vital information that may answer fundamental questions about the origins of autoimmune anti-DNA antibodies in afflicted individuals. The nature of the germline gene populations and the possible microheterogeniety of these genes may prove to be important in understanding the role of autoimmune antibodies in normal and diseased individuals.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Chao M,Voss EW Jrdoi
10.1016/0161-5890(92)90032-ssubject
Has Abstractpub_date
1992-03-01 00:00:00pages
439-42issue
3eissn
0161-5890issn
1872-9142journal_volume
29pub_type
杂志文章abstract：:Osteopontin (OPN) is a cytokine with multiple functions, including the regulation of innate immune response. However, the detailed function and mechanism of OPN in host defense against invaded microorganisms remain unclear. In this report, we revealed that OPN could affect the clearance of Propionibacterium acnes in k...
journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2011-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.08.019
更新日期：2013-03-01 00:00:00
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更新日期：2005-08-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.09.034
更新日期：2009-02-01 00:00:00
abstract：:Egr-1 is an immediate early gene that is rapidly upregulated in response to mitogenic signals induced by antigen receptor crosslinking on murine B lymphocytes. It has been shown that levels of Egr-1 expression are closely correlated with B cell proliferation in several models of B cell activation and tolerance. We com...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90463-l
更新日期：1993-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90110-l
更新日期：1990-02-01 00:00:00
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journal_title：Molecular immunology
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doi：10.1016/j.molimm.2020.06.022
更新日期：2020-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.10.007
更新日期：2006-04-01 00:00:00
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journal_title：Molecular immunology
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doi：10.1016/j.molimm.2007.12.008
更新日期：2008-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/s0161-5890(96)00110-1
更新日期：1997-02-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2020.02.019
更新日期：2020-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90043-4
更新日期：1985-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.01.028
更新日期：2017-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2015.03.246
更新日期：2015-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90180-4
更新日期：1985-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2014.03.013
更新日期：2015-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.11.011
更新日期：2011-01-01 00:00:00
abstract：:Previous work on the cis-acting elements that control heavy chain variable region (VH) gene somatic hypermutation has indicated the presence of an as yet unidentified element(s) 3' of the intron enhancer that is necessary for high rate mutation. Examination of cis-acting elements involved in kappa light chain V gene h...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(97)00065-5
更新日期：1997-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90091-3
更新日期：1983-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.03.018
更新日期：2011-07-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,随机对照试验
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更新日期：2019-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.07.030
更新日期：2006-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.09.031
更新日期：2007-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/s0161-5890(02)00090-1
更新日期：2002-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.04.020
更新日期：2008-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/s0161-5890(02)00059-7
更新日期：2002-09-01 00:00:00
abstract：:Four different proteases were screened for their capability of selectively digesting murine monoclonal IgGl to obtain active F(ab)2. For the screening, a series of five different mouse monoclonal antibodies (IgGl, k) was used, recognizing different tumor-associated antigens and currently used for radioimmunoimaging st...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90088-2
更新日期：1991-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.07.012
更新日期：2010-11-01 00:00:00
abstract：:PIM1 is serine/threonine protein kinase that is involved in numerous biological processes. Pulmonary fibrosis (PF) is a chronic pathological result of the dysfunctional repair of lung injury without effective therapeutic treatments. In the current study, we investigated whether PIM1 inhibition would improve bleomycin ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.06.013
更新日期：2020-09-01 00:00:00
abstract：:Aberrant O-glycosylation of cell surface mucin antigens is characteristic of epithelial cancer cells. For example, Thomsen-Friedenreich disaccharide (TFD) is a chemically well-defined carbohydrate antigen with a documented link to malignancy. There have been many attempts to improve immune response to carbohydrate ant...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(01)00123-7
更新日期：2002-03-01 00:00:00
