Abstract：
:The monocyte chemoattractant protein-1 (MCP-1) functions to recruit monocytes and macrophages to areas of inflammation and is a prototypic chemokine subjected to coordinate regulation by immunomodulatory agents. TNF mediated regulation of MCP-1 occurs through a distal regulatory region located 2.5 kb upstream of the transcriptional start site. Within this region are two NF-kB motifs that are each critical for function. Site A, located within the distal regulatory region and upstream of the kappaB elements is required for maximal induction by TNF. However, unlike the kappaB elements and other MCP-1 regulatory elements, Site A is constitutively occupied by factors in vivo. To better understand the nature of Site A function, this report identified a Site A binding protein and provides a functional analysis of the element in driving transcription. The results showed that the transcription factor NF1/CTF binds to Site A both in vitro and in vivo. While Site A has no transcriptional activity on its own, it was found to augment the transcriptional activity of a GAL4-VP16 reporter system in an orientation and position independent manner. Because NF1 is known to interact with factors that modify nucleosomes, these results suggest a unique role for Site A in regulating MCP-1 expression.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Kumar SN,Boss JMdoi
10.1016/s0161-5890(00)00097-3subject
Has Abstractpub_date
2000-08-01 00:00:00pages
623-32issue
11eissn
0161-5890issn
1872-9142pii
S0161589000000973journal_volume
37pub_type
杂志文章abstract：:A number of fragments derived from acid-treated rabbit IgG by digestion with plasmin have been separated by high-resolution gel filtration. Fragments isolated included a dimer and monomer Facb, named F(acb)2 and Facb, respectively and a heterodimer composed of Facb and Fab subunits, named F(acb)(ab). A C gamma 2 fragm...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90147-6
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abstract：:Meningococcal factor H-binding protein (fHbp) is a promising vaccine antigen. Previous studies described three fHbp antigenic variant groups and identified amino acid residues between 100 and 255 as important targets of variant-specific bactericidal antibodies. We investigated residues affecting expression of an epito...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.02.021
更新日期：2009-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(99)00122-4
更新日期：1999-12-01 00:00:00
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journal_title：Molecular immunology
pub_type： 评论,信件
doi：10.1016/j.molimm.2008.02.015
更新日期：2008-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.11.014
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.06.008
更新日期：2014-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.10.002
更新日期：2013-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(98)00094-7
更新日期：1998-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.09.037
更新日期：2010-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2010-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2015-02-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
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更新日期：2004-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2018.08.001
更新日期：2018-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.molimm.2015.06.003
更新日期：2015-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90156-6
更新日期：1993-08-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.06.015
更新日期：2020-08-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2009.10.008
更新日期：2010-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90144-z
更新日期：1993-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90106-2
更新日期：1983-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2020.02.015
更新日期：2020-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.10.025
更新日期：2007-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2019-10-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.09.020
更新日期：2005-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.09.022
更新日期：2008-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90078-3
更新日期：1989-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90152-p
更新日期：1990-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90089-7
更新日期：1987-02-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.08.018
更新日期：2007-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90045-9
更新日期：1987-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.01.029
更新日期：2007-05-01 00:00:00
