Abstract：
:Rearrangements in reading frame 2 promote the expression of a truncated heavy chain, the Dmu protein. Dmu can assemble into a pre-B cell receptor like complex that appears to induce a subset of signals elicited by full length mu, but cannot promote the pro-B to pre-B cell transition of Rag-/- B cells. In order to determine if this could stem from an impaired survival signal not properly induced by the Dmu protein, we introduced Bcl-2 into Dmu-transgenic, Rag2-/- mice. Despite the fact that the Bcl-2 transgene expression promoted some increase in the fraction of CD43- B cells, an identical increase was also observed in Rag2-/- mice. Moreover, whereas in mu-transgenic Rag2-/-Bcl-2+ mice, CD2 and CD25 expression were up regulated and c-Kit was down regulated, these markers were unaltered in Dmu-transgenic Rag2-/- Bcl-2+ mice compared to Rag2-/- Bcl-2+ mice, indicating that Dmu cannot support pre-B cell maturation despite extended survival of B cell precursors by Bcl-2. In addition, we observed that in Dmu-transgenic recombination competent mice, the Dmu induced partial block is permissive for marginal zone B cell development whereas the formation of follicular B cells is severely reduced. While the Dmu protein is expressed in peripheral B cells escaping the block, only a minor fraction of Dmu is exposed to the outer cell surface.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Wikström I,Bergqvist I,Holmberg D,Forssell Jdoi
10.1016/j.molimm.2005.09.020subject
Has Abstractpub_date
2006-03-01 00:00:00pages
1316-24issue
9eissn
0161-5890issn
1872-9142pii
S0161-5890(05)00339-1journal_volume
43pub_type
杂志文章abstract：:The three-dimensional structure of the Mcg lambda-type Bence-Jones dimer crystallized in ammonium sulfate is known at 2.3-A resolution. A series of nine other human lambda-chains and two kappa-chains did not crystallize under the same conditions. After these proteins were hybridized with the Mcg light chain by the met...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(85)80001-8
更新日期：1985-02-01 00:00:00
abstract：:Regarding the role of micro RNAs (miRNA) in the proliferation and differentiation of T cells as well as the controversy around the role of bacteria in the pathogenesis of abdominal aortic aneurysm (AAA), the effects of Helicobacter pylori (Hp) and Lactobacillus acidophilus (La) were investigated in the induction of mi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.11.007
更新日期：2021-02-01 00:00:00
abstract：:The cell-surface receptor ST2L triggers cytokine release by immune cells upon exposure to its ligand IL-33. To study the effect of ST2L-dependent signaling in different cell types, we generated antagonist antibodies that bind different receptor domains. We sought to characterize their activities in vitro using both tr...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.05.019
更新日期：2016-07-01 00:00:00
abstract：:Although the lambda-bearing antibodies represent only 5% of the total mouse serum immunoglobulins, some antigens such as B1355 dextran (alpha (1-3)Dex), the 4-hydroxy-3-nitrophenyl acetyl (NP) and 2,4-dinitro or 2,4,6-trinitrophenyl (DNP/TNP) antigens can induce lambda-positive immune responses. In contrast to the lam...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90061-2
更新日期：1988-02-01 00:00:00
abstract：BACKGROUND:Angioedema diagnosis classically targets the complement system (via C1 inhibitor (C1Inh) function and antigenic C4 level) and contact phase activation (via amidase activity). Bradykinin is responsible for angioedema attacks and is produced from contact phase activation secondary to failed C1Inh control. OBJ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.01.028
更新日期：2017-05-01 00:00:00
abstract：:The recovery of VDJ rearrangement is most often accomplished by PCR amplification of DNA extracted from mixtures of B-cells. Using this procedure in swine, VDJs containing chimeric V(H) genes that resemble gene-conversion products, are frequently encountered. To examine whether these chimeras could be the result of PC...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/s0161-5890(01)00022-0
更新日期：2000-12-01 00:00:00
abstract：:The structural characteristics of antigens recognized by a panel of monoclonal antibodies prepared against a rabbit T-lymphocyte cell line have been investigated. Those antigens which could be isolated using immunoadsorbents prepared from the monoclonal antibodies had mol. wts of 42,000, 90,000 and 120,000. The 42,000...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(84)90094-4
更新日期：1984-01-01 00:00:00
abstract：:The ragweed allergens Amb t 5 and Amb a 5 are among the smallest inhaled protein allergens known, containing a single, immunodominant T-cell epitope. In this study we analyzed the B-cell epitope structure of Amb t 5. The three-dimensional structures of Amb t 5 and Amb a 5 have been determined by NMR spectroscopy, prov...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(99)00054-1
更新日期：1998-06-01 00:00:00
abstract：:The effect of antibody affinity on molecular forms of immune complexes was investigated by measuring antigen-antibody interactions using surface plasmon resonance (SPR), electrospray ionization time-of-flight mass spectrometry under non-denaturing conditions (MS), analytical ultracentrifugation (AUC), and transmission...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.09.009
更新日期：2009-12-01 00:00:00
abstract：:The complement system is a vital component of the immune-priveliged human eye that is always active at a low-grade level, preventing harmful intraocular injuries caused by accumulation of turnover products and controlling pathogens to preserve eye homeostasis and vision. The complement system is a double-edged sword t...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2017.05.028
更新日期：2017-09-01 00:00:00
abstract：:Selection of specific antibodies from large repertoires is of importance in generating antibodies to specific structural determinants and in studying structure-function relationships. Alkaline phosphatase (AP) has several isozymes with various degrees of homology and a range of common synthetic substrates. We have pre...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.02.024
更新日期：2007-01-01 00:00:00
abstract：:Salmonella enterica serovar Typhimurium (S. Typhimurium) changes the structure of its lipopolysaccharide (LPS) in response to the environment. The two main LPS variants found in S. Typhimurium correspond to LPS with a hepta-acylated lipid A (LPS 430) and LPS with modified phosphate groups on its lipid A (LPS 435). We ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.03.003
更新日期：2019-07-01 00:00:00
abstract：:In the previous study, antibodies against CD3 molecule have been produced and were used in labeling T cells in Japanese flounder (Paralichthys olivaceus). In this paper, CD4+ and CD8+ lymphocytes subpopulations in peripheral blood leucocytes (PBL), spleen and head kidney of flounder were investigated. The flounder CD4...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.02.015
更新日期：2017-05-01 00:00:00
abstract：:A new sulfhydryl redox model of antibody-dependent endocytosis is proposed. This model synthesizes many previous studies employing a variety of experimental approaches. Predictions of this model can be tested experimentally. ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90088-4
更新日期：1985-08-01 00:00:00
abstract：:Chlorophyllin (CHL) was earlier shown to reduce the level of intracellular ROS and apoptosis induced by ionizing radiation and 2,2'-azobis(2-propionimidinedihydrochloride) (AAPH). In the present studies, the effect of CHL on radiation-induced immunosuppression and modulation of immune responses in mice was examined. C...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.02.031
更新日期：2007-01-01 00:00:00
abstract：:The carbohydrate binding properties of a novel member of the subfamily of galactose-specific jacalin-related lectin isolated from the bark of black mulberry (Morus nigra) (Morniga G) was studied in detail by enzyme-linked lectinosorbent and inhibition assays using panels of monomeric saccharides, mammalian polyvalent ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.02.017
更新日期：2007-01-01 00:00:00
abstract：:CD8+ cytolytic T lymphocytes are essential players of anti-tumor immune responses. On tumors, they recognize peptides of about 8-to-10 amino acids that generally result from the degradation of cellular proteins by the proteasome. Until a decade ago, these peptides were thought to solely correspond to linear fragments ...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2018.03.030
更新日期：2019-09-01 00:00:00
abstract：:Optineurin (Optn) is an adaptor protein with homology to NF-κB essential modulator (NEMO), the regulatory subunit of the IκB kinase (IKK) complex. Dysregulation of Optn has been linked to neurodegenerative, autoimmune and bone diseases. Optn shares a high degree of homology with NEMO, but is not part of the same high-...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.01.027
更新日期：2017-05-01 00:00:00
abstract：:This study was undertaken to investigate the nature and microheterogeneity of the carbohydrate moiety of the Fc epsilon receptors of RBL-CA10 and RBL-CA10.7 cells. Treatment using the glycosylation processing inhibitors, castanospermine (CN), 1-deoxymannojirimycin (DMJ), and swainsonine (SW) resulted in a decrease of ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90060-o
更新日期：1993-02-01 00:00:00
abstract：:We have developed two distinct solid-phase immunoassays for the detection of antigen binding activity by products of antigen binding T cell hybridomas in the absence of MHC. Two suppressor T cell hybridomas studied (34s-18 and 34s-704) are specific for keyhole limpet hemocyanin, a protein antigen, and the other suppre...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90043-6
更新日期：1989-07-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.02.019
更新日期：2009-05-01 00:00:00
abstract：:Complement per se has been shown to play an important role in demyelinating disease but controversy remains regarding the role of C3 in the development and progression of experimental autoimmune encephalomyelitis (EAE), the animal model for multiple sclerosis. In this study, we used C3(-/-) mice to confirm previous fi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.02.002
更新日期：2007-05-01 00:00:00
abstract：:We have previously used immunoelectron microscopy (IEM) to generate a three-dimensional map of idiotypic (Id) and isotypic epitopes on the Fab arms of HGAC 39 (Roux et al., 1987, Proc. natn. Acad. Sci. U.S.A. 84, 4984-4988), a mouse IgG3 monoclonal antibody (Mab). In this report, we analyse the geometry of the various...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90167-8
更新日期：1994-06-01 00:00:00
abstract：:Previous data suggested that constitutive expression of the transcription factor Bright (B cell regulator of immunoglobulin heavy chain transcription), normally tightly regulated during B cell differentiation, was associated with autoantibody production. Here we show that constitutive Bright expression results in skew...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.09.008
更新日期：2011-10-01 00:00:00
abstract：:Dendritic cells (DCs) play an important role in antigen presentation, which is an essential step for the induction of antigen-specific adaptive immunity. Inactivated bacterial whole cell vaccines have been widely used to prevent many bacterial infections because they elicit good immunogenicity due to the presence of v...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2018.07.023
更新日期：2018-09-01 00:00:00
abstract：:Two recombinant mouse mammary tumor virus (MMTV) subunit vaccines have been constructed, in which linear sequences of the envelope gp 52 glycoprotein (EP3) or the superantigen (SAg) have been fused to single or multiple repeats of a T-cell epitope (P30) from tetanus toxin. Histidine tags or glutathione-S-transferase (...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(96)00068-5
更新日期：1996-09-01 00:00:00
abstract：:The specificities of the combining sites of 19 mouse monoclonal antibodies to dextran B1355S have been characterized immunochemically by quantitative precipitin and precipitin inhibition assays; association constants for B1355S were determined by affinity gel electrophoresis. Cross-reactive and individual idiotypes re...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90139-2
更新日期：1986-04-01 00:00:00
abstract：:Epithelial surfaces constitute natural immunobarriers against environmental threats. These barriers are brimming with fluids that bind, transport, cleave or degrade bacterial cells and their endotoxic by-products. Saliva and the airway surface-lining fluid (ASL) comprise the important fluid constituents. Short palate,...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.10.021
更新日期：2006-04-01 00:00:00
abstract：:The general thinking about the phylogenic distribution of vertebrate Ig classes is that fish and urodele amphibians are only able to synthesize polymeric IgM-like molecules and that the emergence of a new class of LMW Ig occurs for the first time in anouran species. Following immunization of the Mexican axolotl (Ambys...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90054-5
更新日期：1988-12-01 00:00:00
abstract：:Naïve T-cell activation requires signals from both the T-cell receptor (TCR) and the costimulatory molecule CD28. A central mediator of the TCR and CD28 signals is the scaffold protein CARMA1, which functions by forming a complex with partner proteins, Bcl10 and MALT1. A well-known function of the CARMA1 signaling com...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2015.07.024
更新日期：2015-12-01 00:00:00
