NCCRP-1: a novel receptor protein sequenced from teleost nonspecific cytotoxic cells.


:The recognition structure responsible for binding to conventional antigen on target cells has not previously been described for nonspecific cytotoxic cells (NCC) or for mammalian natural killer (NK) cells. Although several biochemical pathways may be available for initiation of the lytic cycle in NCC, evidence presented indicates that initial contact with a tumor cell or protozoan parasite is facilitated by recognition of a target antigen by a membrane protein of Mr 34,000 on NCC (NCC receptor protein, NCCRP-1). Binding to NCCRP-1 by monoclonal antibody 5C6, by target cell antigen or by cognate synthetic peptide initiates a signalling response leading to increased cytotoxicity. In the present study, three 20-mer microsequences were obtained from tryptic digests of purified NCCRP-1. Degenerate primers were synthesized (based on each peptide sequence) and were used for RT-PCR with mRNA purified from homogeneous NCC populations. An NCCRP-1 specific cDNA sequence was used to synthesize nondegenerate primers. These primers were used in a 5'/3' RACE PCR to obtain the entire NCCRP-1 specific cDNA. A deduced aa sequence consisted of 235 aa with a derived molecular weight of 30,628 Da. NCCRP-1 is proline rich (9%), has two glycosylation sites and 18% of all amino acids are potential phosphorylation sites (serine, threonine, tyrosine). The identity of the protein was confirmed by finding the previously microsequenced peptides in the derived sequence. Homology searches revealed that NCCRP-1 is a novel protein. Northern blot analysis of mRNA content from teleost NCC, B-cells and T-cells revealed only one band in NCC preparations. Functional studies demonstrated a decrease in membrane NCCRP-1 expression and inhibition of NCC cytotoxicity following treatment with NCCRP-1 anti-sense oligonucleotides. Treatment of NCC with sense oligonucleotides had no inhibitory effects on cytotoxicity. An algorithm predicting the membrane conformation of NCCRP-1 suggests one extracellular proline-rich domain, a transmembrane portion of 15 18 aa and a cytoplasmic tail composed of a high frequency of phosphorylation sites. Current studies suggest that NCC and NCCRP-1 may participate in innate resistance functions in teleost fish.


Mol Immunol


Molecular immunology


Jaso-Friedmann L,Leary JH 3rd,Evans DL




Has Abstract


1997-08-01 00:00:00












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    abstract::Radioimmunochemical studies on the comparison of the immunological cross-reactivity between the 7-S Igs of birds, reptiles and amphibians (IgY-like Igs) and the IgA of mammals (man and pig) using 125I-chicken IgY and anti-chicken IgY(Fc) or anti-chicken IgY(H) antibodies from rabbits and carp for the detection led to ...

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    authors: Hädge D,Ambrosius H

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    abstract::Membrane immunoglobulins are integral proteins on B cell surfaces that bind foreign antigens and are critically involved in the regulation of the immune response. Based upon the model of serum IgG, it has been assumed that membrane immunoglobulins are essentially four chain disulfide-linked structures of the form H2L2...

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    abstract::Monoclonal antibodies to cyclosporine A (Cs), a potent immunosuppressant, were generated in BALB/c mice using a novel antigen prepared by linking Cs to a protein carrier via a photoactive cross-linking reagent, 4-benzoylbenzoic acid (BBa). Twenty-two monoclonal anti-Cs antibodies were generated, using Cs-BBa-bovine se...

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    authors: Cacalano NA,Aggarwal R,Quesniaux VF,Cleveland WL,Erlanger BF

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  • Increased expression of IL-18 in the serum and islets of type 1 diabetics.

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    abstract::Anti-human C1s monoclonal antibody H1532, a mouse gamma-1-immunoglobulin elicited by a C1r2C1s2 immunogen, appeared to bind to the beta-domain of C1s by electron microscopy. In agreement with this observation, Western blotting demonstrated good binding to unreduced C1s, but no binding to the alpha or gamma-B domains. ...

    journal_title:Molecular immunology

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    authors: Tseng Y,Phillips ML,Schumaker VN

    更新日期:1997-06-01 00:00:00

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    pub_type: 杂志文章


    authors: Bhakdi S,Tranum-Jensen J

    更新日期:1982-09-01 00:00:00

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  • 1,25-Dihydroxyvitamin D3 induces a myelomonocytic phenotype with enhanced effector cell function in the HL-60 promyelocytic leukemia cell line.

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    abstract::A high affinity C5 convertase is generated when a C3 convertase deposits additional C3b molecules on and around itself thereby switching the substrate specificity of C3 convertase from C3 to C5. In the present study the role of the additional C3b molecules in influencing the regulation of classical pathway C5 converta...

    journal_title:Molecular immunology

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    authors: Rawal N,Pangburn MK

    更新日期:2007-02-01 00:00:00

  • BRAF splice variants in rheumatoid arthritis synovial fibroblasts activate MAPK through CRAF.

    abstract::Rheumatoid arthritis (RA) is a destructive polyarthritis in which synovial-like fibroblasts (SFs) invade and erode cartilage by expressing membrane-anchored type 1 matrix metalloproteinase (MT1-MMP). The mitogen activated protein kinase (MAPK) pathway is activated in RA SFs, but the mechanism of activation is unknown....

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    abstract::T-cell activation requires interaction of T-cell receptors (TCR) with peptide epitopes bound by major histocompatibility complex (MHC) proteins. This interaction occurs at a special cell-cell junction known as the immune or immunological synapse. Fluorescence microscopy has shown that the interplay among one agonist p...

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    authors: Wan S,Flower DR,Coveney PV

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    pub_type: 杂志文章


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  • Innate immune defence in the human gastrointestinal tract.

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    authors: Dommett R,Zilbauer M,George JT,Bajaj-Elliott M

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    pub_type: 杂志文章


    authors: Li Y,Yu C,Zhu WM,Xie Y,Qi X,Li N,Li JS

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    abstract::Dense deposit disease (DDD) is strongly associated with the uncontrolled activation of the complement alternative pathway. Factor H (CFH)-deficient (Cfh(-/-)) mice spontaneously develop C3 deposition along the glomerular basement membrane (GBM) with subsequent development of glomerulonephritis with features of DDD, a ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Paixão-Cavalcante D,Hanson S,Botto M,Cook HT,Pickering MC

    更新日期:2009-06-01 00:00:00

  • Epitope complementarity and idiotypic interactions: a study of idiotypic-like interactions between anti-cytidine and anti-guanosine A/J mouse monoclonal antibodies--I. Characterization of these interactions.

    abstract::Idiotypic-like interactions between mAbs directed against cytidine (Cyd) or guanosine (Guo) nucleosides were characterized. These mAbs, Cyd-1 (IgG2b, kappa), Guo-1 (IgG1, kappa) and Guo-2 (IgG1, kappa) were derived from splenocytes of A/J mice immunized with Cyd-KLH or Guo-KLH and recognized the nucleoside base moieti...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Cottet MH,Denoyelle C,Bordenave G

    更新日期:1994-01-01 00:00:00

  • Autoreactive B-cell repertoire in mice with chronic graft versus host disease.

    abstract::A quantitative analysis of the frequencies of autoantibody producing B-cells has been undertaken by producing and analyzing random hybridoma collections generated in fusions with activated B-cells. Activated B-cells were derived from mice injected with LPS and SRBC and normal mice. They were compared to those derived ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Rolink AG,Thalmann P,Berger C,Radaszkiewicz T,Melchers F

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  • NF-kappaB family of transcription factor facilitates gene conversion in chicken B cells.

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    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Kim Y,Tian M

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    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Polo F,Ayuso R,Carreira J

    更新日期:1990-02-01 00:00:00

  • Role of pH-regulated antigen 1 of Candida albicans in the fungal recognition and antifungal response of human neutrophils.

    abstract::Candida albicans is an opportunistic human-pathogenic fungus, which can cause superficial but also life-threatening invasive infections. The pH-regulated antigen 1 (Pra1) of C. albicans is a surface-associated and secreted protein highly expressed in the hyphal form. Pra1 can bind to complement receptor 3 (CD11b/CD18)...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Losse J,Svobodová E,Heyken A,Hube B,Zipfel PF,Józsi M

    更新日期:2011-09-01 00:00:00

  • Comparison of the antigenicities of native human growth hormone (hGH) and three forms of recombinant hGHs using monoclonal antibodies.

    abstract::A panel of hybridomas producing antibodies specific for human growth hormone (hGH) were prepared by using a recombinant hGH [methionylsomatotropin (r-hGH)] as an immunogen. Thirteen representative monoclonal antibodies which showed different reactivity patterns were used to analyze the antigenicities of four different...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Sawada J,Wada N,Irie M,Tokunaga-Doi T,Ohtsuka E,Ikehara M,Terao T

    更新日期:1986-06-01 00:00:00

  • Interferon-inducible p200-family protein IFI16, an innate immune sensor for cytosolic and nuclear double-stranded DNA: regulation of subcellular localization.

    abstract::The interferon (IFN)-inducible p200-protein family includes structurally related murine (for example, p202a, p202b, p204, and Aim2) and human (for example, AIM2 and IFI16) proteins. All proteins in the family share a partially conserved repeat of 200-amino acid residues (also called HIN-200 domain) in the C-terminus. ...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Veeranki S,Choubey D

    更新日期:2012-01-01 00:00:00