Abstract:
:We have previously developed a number of monoclonal antibodies (Mabs) that bind to protamine. One of these antibodies, Hup1N, binds to human protamine 1 but not to protamine 2. In contrast, Mab HupA binds both protamine 1 and protamine 2. The epitopes for these two Mabs were observed to overlap, and were localized to the evolutionarily conservative amino-terminal region of protamine 1. This assignment is based on antibody binding to protamine from different species in which the protamine sequence is known, as well as analysis of antibody binding to synthetic peptides and synthetic peptides with specific amino acid substitutions.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Stanker LH,Wyrobek A,McKeown C,Balhorn Rdoi
10.1016/0161-5890(93)90436-fsubject
Has Abstractpub_date
1993-12-01 00:00:00pages
1633-8issue
18eissn
0161-5890issn
1872-9142journal_volume
30pub_type
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