Abstract:
:In the present study, we report the activation of murine peritoneal macrophages in vitro on irradiation with sublethal dose of UVB (50 mJ/cm(2)). The activation involves enhanced expression of CD18 molecule and production of nitric oxide (NO), tumor necrosis factor (TNF-alpha) and interleukin-1 (IL-1). Production of NO, TNF-alpha and IL-1 by the macrophages on UVB irradiation was found to peak at 24 h of incubation post UVB irradiation. Increased iNOS, TNF-alpha and IL-1beta mRNAs expression was also observed by reverse transcription and polymerase chain reaction (RT-PCR). The RT-PCR results also showed the increased transcription of IL-6, IL-12, TLR2 and TLR4 genes in UVB-irradiated macrophages. Increased expression of phospho-IkappaB was also observed by immunoblotting in UVB-irradiated macrophages. Investigating the signal transduction pathway responsible for the NO, TNF-alpha and IL-1 production by the UVB-irradiated macrophages, it was observed that the pharmacological inhibitors pertussis toxin, wortmannin, PD98059, SB202190 and genistein blocked NO, TNF-alpha and IL-1 production suggesting the probable involvement of G-proteins, phosphoinositol-3-kinase, p42/44, p38 mitogen activated protein kinases and tyrosine kinases in the above process.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Sethi G,Sodhi Adoi
10.1016/j.molimm.2004.01.001subject
Has Abstractpub_date
2004-04-01 00:00:00pages
1315-23issue
18eissn
0161-5890issn
1872-9142pii
S0161589004000306journal_volume
40pub_type
杂志文章abstract::Stimulation of human tonsillar mononuclear cells with interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) rapidly induced the activation of distinct nuclear factors with different mobilities, both of which bind the IL-4 response element (IL-4RE) of CD23b promoter as examined by electrophoretic mobility shift assays ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(98)00022-4
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doi:10.1016/0161-5890(85)90084-7
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journal_title:Molecular immunology
pub_type: 信件
doi:10.1016/j.molimm.2007.07.005
更新日期:2008-01-01 00:00:00
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journal_title:Molecular immunology
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doi:10.1016/0161-5890(94)90042-6
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
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abstract::Increasing indication is unveiling a role for poly(ADP-ribose) polymerase (PARP)-1 in the regulation of inflammatory/immune responses. The aim of the present study was to determine the potential anti-inflammatory effects of PARP-1 inhibitor 5-aminoisoquinolinone (5-AIQ) to explore the role of PARP-1 inhibitor in a mou...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2014.09.009
更新日期:2015-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.07.009
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更新日期:2014-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.03.001
更新日期:2009-06-01 00:00:00
abstract::The immunological role of exosomes in autoimmune encephalitis (AE) remains uncharacterized and not examined. In this study we ought to determine whether exosomes are generated in AE and to define the presence of cell surface neuronal autoantigens (autoAgs) in the cargo. Exosomes were isolated from cerebrospinal fluid ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.12.034
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pub_type: 杂志文章
doi:10.1016/0161-5890(93)90443-f
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.12.002
更新日期:2020-12-29 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(90)90064-7
更新日期:1990-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2004.03.035
更新日期:2004-07-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2004.11.015
更新日期:2005-05-01 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular immunology
pub_type: 杂志文章
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.01.014
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.09.020
更新日期:2006-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(98)00094-7
更新日期:1998-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(01)00031-1
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(83)90145-1
更新日期:1983-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:1988-11-01 00:00:00