Abstract：
:The translation of mRNA within total RNA of German (Blattella germanica) cockroaches was performed using a rabbit reticulocyte lysate system. Analysis of the translation products by SDS-PAGE and combined autoradiography revealed several synthesized proteins with apparent molecular weights ranging from 20 kD to 110 kD. SDS-PAGE/Western blotting of non-radiolabeled translation products and incubation with human serum with IgE to cockroach allergens showed the presence of a 36 kD and 50 kD allergen. The confirmation of the translation of the cockroach allergens from total RNA is an important first step in the cloning of cockroach allergens.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Helm R,Cockrell G,Sharkey P,Brenner R,Burks AWdoi
10.1016/0161-5890(93)90443-fsubject
Has Abstractpub_date
1993-12-01 00:00:00pages
1685-8issue
18eissn
0161-5890issn
1872-9142journal_volume
30pub_type
杂志文章abstract：:Peptide gpMBP72-85, containing amino acids 72-85 of guinea pig myelin basic protein is commonly used to induce experimental autoimmune encephalomyelitis in Lewis rats. The N-terminal glutamine in this peptide can cyclize to pyroglutamic acid, leading to loss of the first MHC anchor for binding to MHC class II. Acetyla...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2003.10.015
更新日期：2004-02-01 00:00:00
abstract：:Anti-DNP activity was found in normal human immunoglobulins with average affinities (10(4)-10(6) l/mole) and frequencies (15-2%) that correlate well with those expected from multispecific binding sites. ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(84)90143-3
更新日期：1984-10-01 00:00:00
abstract：:Bradykinin (BK) is an inflammatory mediator, and shows elevated levels in regions of severe injury and inflammatory diseases. It has been shown to induce interleukin-6 (IL-6) expression in inflammatory responses in rheumatoid arthritis. We investigated the signaling pathway involved in IL-6 production caused by BK in ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.06.007
更新日期：2008-08-01 00:00:00
abstract：:Cytohesin-1 is a guanine nucleotide exchange factor for ADP ribosylation factor 6 (Arf6) in human blood neutrophils and differentiated PLB-985 neutrophil-like cells. Cytohesin-1 regulates adhesion and the transendothelial migration of monocytes, dendritic cells and T lymphocytes through activation of the β2 integrin L...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.03.018
更新日期：2011-07-01 00:00:00
abstract：:Toll-like receptors (TLRs) are essential receptors of the innate immune system and are first responders for protection against bacterial and viral pathogens. Recently, several TLRs have also been implicated in regulating cell death and survival in non-pathogen injuries such as stroke and oxidative stress. Investigatin...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.11.005
更新日期：2013-06-01 00:00:00
abstract：:A relatively large number of variable region genes (V) contribute, via gene rearrangements with smaller numbers of additional gene elements (D and J), to generate diversity in the immune response. While some VH gene families are thought to contain 100- 1000 members, the VH10 family has only two known functioning membe...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90032-s
更新日期：1992-03-01 00:00:00
abstract：:The MHC class II transactivator (CIITA) acts in the cell nucleus as the master regulator of MHC class II (MHC II) gene expression. It is important to study CIITA regulation in multiple myeloma since MHC expression is central to ability of myeloma cells to present antigen and to the ability of the immune system to reco...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.01.009
更新日期：2007-04-01 00:00:00
abstract：:Structurally diverse dextrans from Leuconostoc mesenteroides and related bacteria have been used extensively in fundamental immunochemical studies such as induction and characterization of anti-dextran antibodies, as well as in studies of their interaction with pneumococcal antisera, normal bovine serum, concanavalin ...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/0161-5890(86)90131-8
更新日期：1986-09-01 00:00:00
abstract：:N-substituted benzamides are compounds that have recently been reported to inhibit nuclear factor-kappaB (NF-kappaB) activity and induce apoptosis in a pre-B cell line. In this study, we focused on the effects of N-substituted benzamides on transcriptional regulation in Jurkat T cells. We used a model system where the...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(01)00060-8
更新日期：2001-08-01 00:00:00
abstract：:Low molecular weight hemorrhagins were purified from crude Bothrops atrox snake venom by gel filtration followed by ionic strength chromatography. The protein fractions obtained, designated HI-1 to HI-8, contained proteins with molecular masses lower than 30 kDa. HI-5, the most representative among of these fractions,...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2003.11.032
更新日期：2004-03-01 00:00:00
abstract：:GK-1 is an immunomodulatory, 18-aa-long peptide that has been proved to promote the activation of mouse peritoneal macrophages and LPS-pulsed mouse bone marrow-derived dendritic cells (BM-DCs). This study is aimed to explore the mechanisms underlying the activation of these antigen-presenting cells (APCs) by GK-1. In ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.09.015
更新日期：2020-12-01 00:00:00
abstract：:A bovine papilloma virus-derived vector was used to direct the high level expression in mouse C127 cells of three different cDNAs encoding the human interleukin-2 receptor. These were: the previously described cDNA clone isolated from the T-cell lymphoma, HUT-102; a cDNA clone isolated from mitogen-activated, normal p...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90123-9
更新日期：1986-09-01 00:00:00
abstract：:Hyperimmune absorbed rabbit antisera which were reactive with epitopes specific for individual variants of human placental alkaline phosphatase were tested for their reactivity with primate placental alkaline phosphatases. Using the three epitope-specific reactivities defined previously, we found that: epitope I is pr...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(84)90074-9
更新日期：1984-03-01 00:00:00
abstract：:The role of secondary structures and base mutability at different levels of transcription and supercoiling is analyzed in variable region antibody genes VH5, VH94 and VH186.2. The data are consistent with a model of somatic hypermutation in which increasing levels of transcription and secondary structure stability cor...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.05.012
更新日期：2008-08-01 00:00:00
abstract：:Pulmonary exposure to innocuous aeroallergens is a common event leading to inhalation tolerance. Distinct subsets of pulmonary dendritic cells (DC) and regulatory T cells (T(Reg)) play critical roles in mediating and maintaining such tolerance. In asthmatics, the same aeroallergens drive a maladaptive, Th2-biased immu...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2006.06.016
更新日期：2007-01-01 00:00:00
abstract：:Earlier studies, which provided indirect evidence for the involvement of the C gamma 2 domain of human immunoglobulin G (IgG) in human immunoglobulin G (IgG) in human monocyte binding, have been extended to further localise the site of interaction on human IgG. A number of IgGs from several different species and fragm...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90059-3
更新日期：1986-03-01 00:00:00
abstract：:In this study, a novel approach for the development of a peptide-based vaccine has been tested. We investigated the possibility of replacing an all-L amino acid peptide sequence corresponding to the protective B-cell epitope hemagglutinin (HA) 91-108 from influenza HA with a retro-inverso analogue encompassing this se...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(02)00095-0
更新日期：2002-10-01 00:00:00
abstract：:Human complement components C6 and C7 have been purified and characterized. Component C6 is a single-chain plasma protein of mol. wt 104,800 containing 3.8% carbohydrate and it has alanine as the amino terminal residue. Component C7 is a single-chain plasma protein of mol. wt 92,400 containing 6.4% carbohydrate and it...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90189-4
更新日期：1982-11-01 00:00:00
abstract：:Peptides representing the amino-terminal sequence of protamines HP1 (sequence 1-12) and HP2 (sequence 1-11), the two major nuclear proteins of human sperm, have been synthesized. Rabbits were immunized either with peptide conjugated with a carrier or with free peptide. The resulting antisera were examined for their ca...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90179-n
更新日期：1991-09-01 00:00:00
abstract：:Major histocompatibility complex (MHC) class II molecules are cell surface glycoproteins and are known to display processed antigens on the surface of antigen presenting cells (APC). Within the APC, the loading of processed antigenic peptides to MHC class II molecules is known to take place in the endosomal compartmen...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00030-i
更新日期：1995-06-01 00:00:00
abstract：:Recent reports demonstrate that natural killer (NK) cells and dendritic cells (DC) support each other's activity in a positive feedback. We observed that activated NK cells induce the maturation of DCs into stable type-1 polarized DCs (DC1), characterized by up to 100-fold enhanced ability to produce IL-12p70 in respo...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2004.07.038
更新日期：2005-02-01 00:00:00
abstract：:Cell-cell signaling represents an essential hallmark of multicellular organisms, which necessarily require a means of communicating between different cell populations, particularly immune cells. Cytokine receptor signaling through the Janus kinase/Signal Transducer and Activator of Transcription/Suppressor of Cytokine...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.02.002
更新日期：2016-03-01 00:00:00
abstract：:For decades immunological research has relied, with variable success, on mouse models to investigate diseases and possible therapeutic interventions. With the approval of the first therapeutic antibody targeting complement, called eculizumab, as therapy in paroxysmal nocturnal hemoglobinuria (PNH) and more recently at...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2015.03.011
更新日期：2015-09-01 00:00:00
abstract：:Genetic and functional studies suggest that polymorphism in cytotoxic T lymphocyte-associated antigen-4 (CTLA4) and inducible costimulator (ICOS) genes, both reported to harbour autoimmune susceptibility loci, could regulate the immune activation through affecting their expression and splicing of CTLA4. To address thi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.08.010
更新日期：2007-03-01 00:00:00
abstract：:Sialic acid - binding immunoglobulin - like lectins (Siglecs) are members of the largest superfamily of immune receptors; they recognize sialic acid and are mainly expressed in immune cells. Studies on mammals indicate that Streptococcus agalactiae (GBS) evade immune reactions by interacting with the host immune cells...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.01.005
更新日期：2016-05-01 00:00:00
abstract：:Invasive Salmonella has been reported to induce apoptosis of macrophages as a part of its infection process, which may allow it to avoid detection by the innate immune system. However, the bacterial components capable of inducing apoptosis, particularly under the environments offered by the host have not been fully id...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.08.022
更新日期：2007-03-01 00:00:00
abstract：:We have recently developed an in vitro culture model enabling the large-scale expansion of switched-memory B lymphocytes, producing a polyclonal human IgG repertoire. Given the importance of glycosylation for the functions of immunoglobulins, we analyzed the N-glycosylation profiles of the immunoglobulin G (IgG) in th...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2013.10.005
更新日期：2014-02-01 00:00:00
abstract：:T-cell-replacing factor (TRF)/IL-5 is a T-cell-derived glycoprotein which has pleiotropic activity on lymphoid and myeloid cells. IL-5 polypeptide translated into Xenopus oocytes are heterogeneous in molecular size (40,000 to 60,000 under nonreducing conditions) and yields a monomeric form (Mr of 25,000 to 30,000) und...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90158-v
更新日期：1990-09-01 00:00:00
abstract：:Mannose-binding lectin (MBL) is a recognition molecule of the complement (C) system and binds to carbohydrate ligands present on a wide range of pathogenic bacteria, viruses, fungi, and parasites. MBL has been detected in the cervico-vaginal cavity where it can provide a first-line defence against infectious agents co...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.07.016
更新日期：2010-11-01 00:00:00
abstract：:Johne's disease is a chronic granulomatous enteritis caused by Mycobacterium avium subsp. paratubercolosis (MAP) which affects ruminants worldwide and has a significant economic impact. MAP has also been associated with human Crohn's disease, although this connection is not well established. MAP is highly adapted for ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.08.017
更新日期：2017-11-01 00:00:00
