Abstract：
:We have shown previously that dipeptidyl peptidase 2 (DPP2) activity is essential for the survival of quiescent, but not activated, lymphocytes. The specific requirement of DPP2 activity for non-dividing cells is indicative of cell cycle specific regulation of this gene product. In the present study, we tested this hypothesis by looking at contact and serum dependence of Dpp2 transcription. We found that transfected promoter-reporter activity, as well as endogenous Dpp2 transcripts, were enhanced in NIH-3T3 cells upon contact-inhibition or serum starvation. Since lung Kruppel-like factor (KLF2), a transcription factor, and TOB1, a transcriptional co-activator, have been shown to be important in maintaining T-lymphocyte quiescence and are both downregulated upon cellular activation, we also looked at the contributions of these factors to Dpp2 transcription. Using a Dpp2 promoter-reporter system, we demonstrate that KLF2 and TOB1 activate the mouse Dpp2 promoter. Finally, we show that in human PBMC, there is a decrease in levels of endogenous DPP2 transcripts upon T cell receptor activation when compared to resting cells. These results demonstrate that Dpp2 transcription is serum and contact-dependent and link two quiescence-specific transcriptional elements to the quiescence-specific requirement of DPP2 enzymatic activity.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Bista P,Mele DA,Baez DV,Huber BTdoi
10.1016/j.molimm.2008.05.001subject
Has Abstractpub_date
2008-08-01 00:00:00pages
3618-23issue
13eissn
0161-5890issn
1872-9142pii
S0161-5890(08)00189-2journal_volume
45pub_type
杂志文章abstract：:CD55 is a key regulator of complement activation, expressed on most tissues and cells in man and other mammals. In the rat, alternative splicing in the gene encoding CD55 yields GPI-anchored (GPI-CD55) and transmembrane (TM-CD55) forms. Published Northern blot analysis indicated that while GPI-CD55 was broadly express...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.08.018
更新日期：2007-03-01 00:00:00
abstract：:N-Acetylcysteine (NAC) has been widely used as an antioxidant in research, however, it has also been found to reduce the binding of TNF to its receptor independent of its antioxidative role. In this study, we investigated the effect of NAC on NF-kappaB activation. In HeLa cells, Hep3B cells, and A549 cells, DNA-bindin...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.06.012
更新日期：2008-09-01 00:00:00
abstract：:Regarding the role of micro RNAs (miRNA) in the proliferation and differentiation of T cells as well as the controversy around the role of bacteria in the pathogenesis of abdominal aortic aneurysm (AAA), the effects of Helicobacter pylori (Hp) and Lactobacillus acidophilus (La) were investigated in the induction of mi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.11.007
更新日期：2021-02-01 00:00:00
abstract：:A transplanted organ suffers inherently from an ischaemic insult and subsequent reperfusion injury. The severity of such early events is thought to influence the success of the transplant procedure, not only in the immediate post-transplant period, but also to predispose the graft to both acute and chronic rejection. ...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/s0161-5890(99)00118-2
更新日期：1999-09-01 00:00:00
abstract：:Specific IgE antibodies have been affinity-purified from recombinant grass pollen allergens, and used to identify isoforms of the two major allergens of rye-grass pollen, Lol p I and Lol p V and cross-reactive allergens in other grasses. Lol p I-specific IgE (affinity-purified from the recombinant protein expressed by...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90068-x
更新日期：1994-04-01 00:00:00
abstract：AIM:To study the role of complement receptor 1 (CR1) for binding of Escherichia coli (E. coli) to erythrocytes, for leukocyte phagocytosis, oxidative burst and complement activation in human whole blood from a CR1 deficient (CR1D) patient and healthy controls with low, medium and high CR1 numbers. METHODS:Alexa-labell...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.07.014
更新日期：2019-10-01 00:00:00
abstract：:Rainbow trout (Oncorhynchus mykiss) cells of a monocyte-macrophage lineage (rtMOCs) were used to characterize the ability of the trout innate immune system to recognize and respond to different pathogen associated molecular pattern (PAMP) bearing substances. Compared to what has been reported for mammalian macrophages...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.11.023
更新日期：2005-06-01 00:00:00
abstract：:Bovine and equine factor D were purified to apparent homogeneity as evidenced by a single protein staining band on 7.5-17.5% SDS-PAGE slab gels under both reducing and non-reducing conditions. An apparent mol. wt of 15,000 for bovine D and 22,500 for equine D were noted after SDS-PAGE gel analysis of both reduced and ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(84)90141-x
更新日期：1984-10-01 00:00:00
abstract：:Integrin-mediated adhesion of human monocytes to fibrinogen regulated by CD11b/CD18 and the closely related integrin CD11c/CD18, play a key role in inflammation. Peripheral blood monocytes isolated from human donors despite expressing CD11c primarily utilized CD11b to mediate adhesion to fibrinogen upon stimulation wi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.04.021
更新日期：2008-08-01 00:00:00
abstract：:Four cell lines producing monoclonal antibodies were obtained by fusion of NS1 myeloma cells with splenocytes of BALB/C mice immunized with only 1 microgram of each staphylococcal enterotoxin A, B, C1 and D by a modified technique of intrasplenic boosting. This procedure was considerably more efficient than the more c...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90118-0
更新日期：1987-12-01 00:00:00
abstract：INTRODUCTION:Previous studies have suggested the importance of somatic mutations and arginine, asparagine and lysine residues in the complementarity determining regions (CDRs) of antiphospholipid antibodies (aPL) implicated in the pathogenesis of the antiphospholipid antibody syndrome. The relative contributions of the...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(03)00100-7
更新日期：2003-09-01 00:00:00
abstract：:MicroRNAs (miRNAs) are small noncoding RNAs that function in diverse biological processes. However, little is known about the precise role of microRNAs in the functioning of airway smooth muscle cells (ASMCs). Here, we investigated the potential role and mechanisms of the miR-143 -3p on proliferation and the extracell...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.09.004
更新日期：2016-10-01 00:00:00
abstract：:The potential biochemical mechanisms that mediate the 'shedding' of soluble IL-6 RI (80-kDa) receptor fragments in populations of adherent macrophages were explored. Stimulated macrophages displayed proportional increases in both the expression of membrane-associated IL-6 RI (80-kDa) and the release of soluble recepto...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(01)00074-8
更新日期：2001-09-01 00:00:00
abstract：:The anti-cytokeratin (CK) 8 monoclonal antibody (mab) TS1 has been shown to efficiently bind to CK8 expressed in carcinomas in vivo. The anti-idiotypic antibody of TS1, alphaTS1, can be used to regulate the tumor:non-tumor ratio of TS1 by clearing non-tumor binding TS1 from the circulation. If the interaction of TS1 t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.08.001
更新日期：2007-02-01 00:00:00
abstract：:BST-2 (also known as tetherin, CD317, or HM1.24) was first described as a potent interferon-inducible host antiviral factor nearly five years ago. Since that time, numerous reports have been published regarding the antiviral activity and immunological properties of this protein. BST-2 blocks viral replication by inhib...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2012.11.008
更新日期：2013-06-01 00:00:00
abstract：:Membrane immunoglobulins are integral proteins on B cell surfaces that bind foreign antigens and are critically involved in the regulation of the immune response. Based upon the model of serum IgG, it has been assumed that membrane immunoglobulins are essentially four chain disulfide-linked structures of the form H2L2...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90451-g
更新日期：1993-12-01 00:00:00
abstract：:The monocyte chemoattractant protein-1 (MCP-1) functions to recruit monocytes and macrophages to areas of inflammation and is a prototypic chemokine subjected to coordinate regulation by immunomodulatory agents. TNF mediated regulation of MCP-1 occurs through a distal regulatory region located 2.5 kb upstream of the t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(00)00097-3
更新日期：2000-08-01 00:00:00
abstract：:An attempt was made to obtain fragments containing the C gamma 2 region by selectively cleaving human Fc fragments prepared from pooled IgG at Met residues using cyanogen bromide. Based on the known locations of Met residues in the Fc regions of human IgG subclasses, fragments between Met 252 and 358, comprising the C...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90052-5
更新日期：1985-12-01 00:00:00
abstract：:The T-dependent BALB/c murine immune response to fluorescein (F1) is characterized by structural heterogeneity at the protein level exemplified in part by a significantly wide range of affinities (Ka), and apparent lack of dominant idiotypes. In order to generate an idiotype family, xenogenic anti-idiotype (anti-ID) a...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90072-0
更新日期：1985-08-01 00:00:00
abstract：:Increasing evidence has demonstrated that the epithelial cells in the lung play crucial roles in regulating certain inflammatory responses by modulating Wnt signaling during microbial infection. However, the anti-microbial functions of Wnt signaling in alveolar epithelial cells remain elusive. In this report, we show ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.02.002
更新日期：2014-06-01 00:00:00
abstract：:Natural resistance to infection with intracellular parasites, such as Leishmania, Salmonella, and Mycobacterium, is controlled in mice by the expression of a single dominant gene locus designated Lsh/Ity/Bcg. Natural resistance-associated macrophage protein gene 1 (NRAMP1) was isolated as a candidate gene. NRAMP1 enco...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(96)00088-0
更新日期：1996-11-01 00:00:00
abstract：:Fibroblast-like synoviocyte (FLS) is the ultimate effectual cells in the pathogenesis of rheumatoid arthritis (RA). The current study was undertaken to investigate whether a proliferation-inducing ligand (APRIL) mediates the function of FLS in an animal model of RA. Rat adjuvant-induced arthritis (AA) was induced by i...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.11.002
更新日期：2015-03-01 00:00:00
abstract：:A sheep cDNA library was screened with a human C mu probe, and the complete nucleotide sequence of a 1923 nt cDNA was determined. It contains sequences corresponding to all the exons (VH, DH, JH, CH1, CH2, CH3 and CH4) characteristic of the immunoglobulin mu heavy chain regions. The deduced amino acid sequence shows a...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90120-m
更新日期：1992-07-01 00:00:00
abstract：:A turbidimetric method for measuring the diameters of insoluble immune complexes, based on the wavelength dependence of their ability to scatter light, was developed. The method was validated by demonstrating that it gave experimental values for the diameters of polystyrene microspheres which were in good agreement wi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90074-l
更新日期：1993-05-01 00:00:00
abstract：:Armenian hamsters were immunized with non-liganded, partially liganded or affinity-labeled anti-fluorescein Mab 4-4-20. Seventeen hybridoma producing monoclonal anti-4-4-20 antibodies were characterized from chemically-mediated fusions of immune hamster lymphocytes with murine Sp2/O-Ag14 myeloma cells. Distinct popula...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90125-u
更新日期：1993-08-01 00:00:00
abstract：:The paper evaluates the role of the concept of degeneracy in current immunology. The historical background of the notion is considered, as well as that of related concepts like 'cross-reactivity' and 'promiscuity'. A distinction is made between degeneracy, as a property of a system and promiscuity, as a property of an...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2003.11.021
更新日期：2004-02-01 00:00:00
abstract：:The diagnosis and therapy of allergic disorders are usually performed with crude extracts which are a heterogeneous mixture of proteins with different allergenic potency. The knowledge of the allergenic composition is a key step for diagnostic and therapeutic options. Parietaria judaica pollen represents one of the ma...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.01.009
更新日期：2008-05-01 00:00:00
abstract：:With the exception of a few strains such as BuB/BnJ, complement activity in most inbred strains of mice is remarkably similar. The BuB/BnJ strain reportedly has high levels of complement activity and elevated serum levels of C3 and other complement proteins. However, we observed that BuB/BnJ mice have serum C3 levels ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.10.004
更新日期：2006-04-01 00:00:00
abstract：:Peptide binding to major histocompatibility complex (MHC) class II molecules is optimal at mildly acidic pH. X-ray crystal structures solved for the murine class II molecule I-E(k) revealed an interesting localization of negatively charged residues within the P6 pocket, which may have implications in the pH dependency...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(02)00010-x
更新日期：2002-06-01 00:00:00
abstract：OBJECTIVE:Nuclear components targeted by autoantibodies are a characteristic feature of the autoimmune disease systemic lupus erythematosus (SLE). The nucleosome, a major autoantigen, is released in patients with SLE as a result of a disturbed apoptosis and/or an insufficient clearance of apoptotic debris. During apopt...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.08.003
更新日期：2010-11-01 00:00:00
