Complement in BuB/BnJ mice revisited: serum C3 levels and complement opsonic activity are not elevated.

abstract：:Armenian hamsters were immunized with non-liganded, partially liganded or affinity-labeled anti-fluorescein Mab 4-4-20. Seventeen hybridoma producing monoclonal anti-4-4-20 antibodies were characterized from chemically-mediated fusions of immune hamster lymphocytes with murine Sp2/O-Ag14 myeloma cells. Distinct popula...

journal_title：Molecular immunology

authors： Weidner KM,Denzin LK,Kim ML,Mallender WD,Miklasz SD,Voss EW Jr

更新日期：1993-08-01 00:00:00

abstract：:The antigen-limiting nature of microtiter ELISAs predicts that antibodies of minor classes may be underestimated when the same specimen contains large amounts of IgG antibodies specific for the same antigen. Such competitive inhibition can be diagnosed from ELISA titration plots. A method is described to eliminate the...

journal_title：Molecular immunology

authors： Butler JE,Heo Y,Adams P,Richerson HB

更新日期：1990-04-01 00:00:00

abstract：:alpha 2-Macroglobulin (alpha 2M) complexed with proteinases or modified by the action of amines has been shown to affect immune responses in vitro, though as yet the mechanisms are poorly understood. Supernates from rabbit lymphoid cells cultured in medium with normal rabbit serum and 35S-methionine (or 14C-leucine) w...

journal_title：Molecular immunology

authors： Schlesinger C,McEntire J,Wallman J,Skosey JL,Hanly WC,Teodorescu M

更新日期：1989-03-01 00:00:00

abstract：:In the present report we have determined the molecular mechanisms, which govern the expression of the human IL-10 gene when induced by the glucocorticoid Methyl-Prednisolone (MP). Treatment of cells with MP at 10(-6) M will readily induce IL-10 in CD19+ primary B cells and in a human B cell line. Analysis of the IL-10...

journal_title：Molecular immunology

authors： Unterberger C,Staples KJ,Smallie T,Williams L,Foxwell B,Schaefer A,Kempkes B,Hofer TP,Koeppel M,Lohrum M,Stunnenberg H,Frankenberger M,Ziegler-Heitbrock L

更新日期：2008-06-01 00:00:00

abstract：:One of the major obstacles in the successful clinical application of monoclonal antibodies has been the development of host immune responses to murine Ig constant and variable regions. While the CDR grafting of MAbs may alleviate many of these problems, the potential remains that one or more murine CDRs on the human I...

journal_title：Molecular immunology

authors： Iwahashi M,Milenic DE,Padlan EA,Bei R,Schlom J,Kashmiri SV

更新日期：1999-10-01 00:00:00

abstract：:The complement system is an essential component of the innate immune system that participates in elimination of pathogens and altered host cells and comprises an essential link between the innate and adaptive immune system. Soluble and membrane-bound complement regulators protect cells and tissues from unintended comp...

journal_title：Molecular immunology

authors： Ferreira VP,Pangburn MK,Cortés C

更新日期：2010-08-01 00:00:00

abstract：BACKGROUND:Bladder cancer (BC) can be successfully treated by manipulating immune responses with intravesical Bacillus Calmette-Guerin instillation or targeting the PD-1/PD-L signaling pathway. In the present study we investigated the prognostic significance of the immune checkpoint inhibitor PD-1 and its ligands PD-L1...

journal_title：Molecular immunology

authors： Ariafar A,Ghaedi M,Rezaeifard S,Shahriari S,Zeighami S,Ghaderi A,Faghih Z

更新日期：2020-08-01 00:00:00

abstract：:The transglutaminase 2 (TG2) is one of the enigmatic enzymes with important functional diversity. It plays an important role in several pathologies such as celiac disease (CD). In patients with active CD, the abnormal retrotranscytosis of IgA/gliadin complexes is mediated by Transferrin Receptor 1 (TfR1). This triad a...

journal_title：Molecular immunology

authors： Abbad L,Monteiro RC,Berthelot L

更新日期：2020-05-01 00:00:00

abstract：:The specificity of a mouse anti-testicular cell monoclonal antibody, J1, was investigated. Previous studies suggested that N-acetyl-D-glucosamine (GlcNAc) was a constituent of the determinant recognized by J1. When the antibody was tested against a variety of purified glycolipids containing this saccharide in terminal...

journal_title：Molecular immunology

authors： Symington FW,Fenderson BA,Hakomori S

更新日期：1984-10-01 00:00:00

abstract：:Despite the fact that receptors for complement on T-cells have been described many years ago the function remains unclear as is the role of complement in the T-cell response. In this review we will evaluate how the accumulated wisdom concur with the current concepts of the adaptive T-cell response. ...

journal_title：Molecular immunology

authors： Wagner C,Hänsch GM

更新日期：2006-01-01 00:00:00

abstract：:Major histocompatibility complex (MHC) class I and II proteins have been in the focus of interest for immunologists, biochemists, cell biologists, and structural biologists for decades. With dozens of entries in the Protein Data Bank, their crystal structures are now sufficiently well understood, while their dynamic p...

journal_title：Molecular immunology

authors： Yaneva R,Schneeweiss C,Zacharias M,Springer S

更新日期：2010-01-01 00:00:00

abstract：:Signal transduction by the B cell receptor (BCR) is an absolute requirement for the selection and development of B lymphocytes at multiple checkpoints. Binding to antigen via the BCR is complemented by a co-stimulus delivered through accessory and co-stimulatory cell surface molecules that regulate the signalling thre...

journal_title：Molecular immunology

authors： Galloway TS,Ray K,Malhotra R

更新日期：2003-01-01 00:00:00

abstract：:Monoclonal antibodies were prepared against synthetic peptides synthetized on the basis of nucleotide sequence from cDNA and genomic clones encoding a class I antigen expressed by the rabbit RL-5 cell line. Using a peptide corresponding to positions 61-73 of the N domain, we were able to obtain an hybridoma producing ...

journal_title：Molecular immunology

authors： Le Guern A,Wetterskog D,Marche PN,Kindt TJ

更新日期：1987-05-01 00:00:00

abstract：:Bovine and equine factor D were purified to apparent homogeneity as evidenced by a single protein staining band on 7.5-17.5% SDS-PAGE slab gels under both reducing and non-reducing conditions. An apparent mol. wt of 15,000 for bovine D and 22,500 for equine D were noted after SDS-PAGE gel analysis of both reduced and ...

journal_title：Molecular immunology

authors： Blanchard DB,Leid RW

更新日期：1984-10-01 00:00:00

abstract：:Multiple organ dysfunction syndrome (MODS) is a major cause of morbidity and mortality in surgical intensive care units. It is characterized by progressive failure of two or more organs remote from the origin of injury. Since MODS results from a severe generalized inflammatory response, both chemokines and complement ...

journal_title：Molecular immunology

authors： Miller CG,Cook DN,Kotwal GJ

更新日期：1996-10-01 00:00:00

abstract：:Two recombinant mouse mammary tumor virus (MMTV) subunit vaccines have been constructed, in which linear sequences of the envelope gp 52 glycoprotein (EP3) or the superantigen (SAg) have been fused to single or multiple repeats of a T-cell epitope (P30) from tetanus toxin. Histidine tags or glutathione-S-transferase (...

journal_title：Molecular immunology

authors： Astori M,Kraehenbuhl JP

更新日期：1996-09-01 00:00:00

abstract：:Toll-like receptors (TLRs) are essential receptors of the innate immune system and are first responders for protection against bacterial and viral pathogens. Recently, several TLRs have also been implicated in regulating cell death and survival in non-pathogen injuries such as stroke and oxidative stress. Investigatin...

journal_title：Molecular immunology

authors： Patel AK,Hackam AS

更新日期：2013-06-01 00:00:00

abstract：:In the adult mouse, the earliest thymocytes are derived from bone marrow-resident T lymphocyte precursor (pre-T) cells that immigrate to the thymus. There they undergo maturation through a series of developmental steps that include rearrangement and expression of the TCR genes, positive and negative selection, and fun...

journal_title：Molecular immunology

authors： Wang TG,Lybarger L,Soloff R,Dempsey D,Chervenak R

更新日期：1996-08-01 00:00:00

abstract：:Amyloid-beta (Aβ) plaque accumulation in the brain is one of the hallmarks of Alzheimer's disease (AD). Immunotherapy against Aβ was considered a potential strategy for reducing the Aβ load in the brain. However, none of the Aβ immunotherapies have produced clinically meaningful results to date, due to poor safety or ...

journal_title：Molecular immunology

authors： Fu L,Guo Y,Sun Y,Dong Y,Wu J,Yu B,Zhang H,Yu X,Wu H,Kong W

更新日期：2018-09-01 00:00:00

abstract：:The T cell receptor (TCR) detects the presence of infectious pathogens and activates numerous intracellular signaling pathways. Protein tyrosine phosphorylation and ubiquitination serve as key regulatory mechanisms downstream of the TCR. Negative regulation of TCR signaling pathways is important in controlling the imm...

journal_title：Molecular immunology

authors： Carpino N,Chen Y,Nassar N,Oh HW

更新日期：2009-10-01 00:00:00

abstract：:Pathogen-driven selection is believed to be important in the evolution and maintenance of the polymorphism of the major histocompatibility complex (MHC) genes but have been tested for very few vertebrates. In this study, we first investigate by SSCP (single strand conformational polymorphism) the diversity found at th...

journal_title：Molecular immunology

authors： Croisetière S,Tarte PD,Bernatchez L,Belhumeur P

更新日期：2008-06-01 00:00:00

abstract：:Natural resistance to infection with intracellular parasites, such as Leishmania, Salmonella, and Mycobacterium, is controlled in mice by the expression of a single dominant gene locus designated Lsh/Ity/Bcg. Natural resistance-associated macrophage protein gene 1 (NRAMP1) was isolated as a candidate gene. NRAMP1 enco...

journal_title：Molecular immunology

authors： Kishi F,Yoshida T,Aiso S

更新日期：1996-11-01 00:00:00

abstract：:Syngeneic polyclonal antibodies were elicited to an affinity labeled high affinity (2-3 X 10(10) M-1) anti-fluorescein murine IgG2a monoclonal antibody. Hyperimmune ascites fluid was tested for reactivity with homologous liganded, affinity labeled and non-liganded Fab fragments derived from the high affinity antibody....

journal_title：Molecular immunology

authors： Voss EW Jr,Miklasz SD,Petrossian A,Dombrink-Kurtzman MA

更新日期：1988-08-01 00:00:00

abstract：:MHC class I molecules present short peptides, usually 8-10 amino acids in length, to CD8(+) T cells. These peptides are typically generated from full-length endogenously synthesized proteins degraded by the antigen processing machinery of the target cell. However, exogenous proteins, whether originating from intracell...

journal_title：Molecular immunology

authors： Wolf BJ,Princiotta MF

更新日期：2011-01-01 00:00:00

abstract：:The high affinity receptor for IgE, which mediates allergic reactions, is found exclusively on mast cells, basophils and epidermal Langerhans cells. It is composed of an alpha, beta and two gamma-polypeptide chains, inserted as a complex into the plasma membrane. The alpha and beta-subunits are produced only by these ...

journal_title：Molecular immunology

authors： Witthuhn BA,Faust C

更新日期：1996-07-01 00:00:00

abstract：:The number of V alpha and V beta sequences of T cell receptors now available allows a meaningful analysis of their variability profiles. Variability plots were derived using a modified form of Wu and Kabat's algorithm: variability is not computed as a proportion of the number of different residues occurring at a posit...

journal_title：Molecular immunology

authors： Wuilmart C,Urbain J

更新日期：1991-09-01 00:00:00

abstract：BACKGROUND:Tumor necrosis factor-α (TNF-α) is involved in inflammatory responses in atherosclerosis. We propose an in vitro cellular assay to evaluate the anti-inflammatory mechanisms of potential modifiers such as food extracts. In the current model we assessed an anti-inflammatory effect of polyphenol-rich peanut ext...

journal_title：Molecular immunology

authors： Catalán Ú,Fernández-Castillejo S,Anglès N,Morelló JR,Yebras M,Solà R

更新日期：2012-10-01 00:00:00

abstract：:Two anti-phenyloxazolone (phOx3) and one anti-GAT MAbs from C57BL mice are shown to be coded by VH gene 186.2. This gene has been found earlier to code for several anti-NP (NNP) antibodies (Bothwell et al., 1981) and anti-GT antibodies (Rocca-Serra et al., 1983; Carmack and Pincus, 1986). The L chain partner of the VH...

journal_title：Molecular immunology

authors： Kaartinen M,Rocca-Serra J,Mäkelä O

更新日期：1988-09-01 00:00:00

abstract：:NKG2D receptor-ligand interaction triggers NK cell-mediated cytolysis and IFN-gamma secretion. IFN-gamma produced by NK cells has been found to promote the interaction between NK cells and monocytes; however, the underlying mechanism remains elusive. We demonstrate here that IFN-gamma exclusively induced or upregulate...

journal_title：Molecular immunology

authors： Wang H,Ruan Z,Wang Y,Han J,Fu X,Zhao T,Yang D,Xu W,Yang Z,Wang L,Chen Y,Wu Y

更新日期：2008-03-01 00:00:00

abstract：:We recently identified IgE-binding epitopes of bovine serum albumin (BSA), the major beef allergen, and found that the major epitope structure contains an EXXV motif. Here we report hypoallergenic analogue peptides of BSA that preserve T cell reactivity to retain the capacity to induce immunotolerance. Substitution of...

journal_title：Molecular immunology

authors： Tanabe S,Shibata R,Nishimura T

更新日期：2004-07-01 00:00:00