Complement in BuB/BnJ mice revisited: serum C3 levels and complement opsonic activity are not elevated.

abstract：:Chagas' disease, caused by Trypanosoma cruzi, is an inflammatory disorder leading to chronic Chagas cardiomyopathy (CCC). Only one third of T. cruzi-infected individuals progress to CCC while the others are considered asymptomatic (ASY). The human inhibitory kappaB-like gene (IKBL/NFKBIL1), homologous to the IkappaB f...

journal_title：Molecular immunology

authors： Ramasawmy R,Faé KC,Cunha-Neto E,Borba SC,Ianni B,Mady C,Goldberg AC,Kalil J

更新日期：2008-01-01 00:00:00

abstract：:Mice were immunized with a purified preparation of human alpha-foetoprotein (AFP) and their spleen cells were hybridized with mouse myeloma cells. Eleven hybridoma cell lines secreting antibody to AFP were obtained. These antibodies were classified into two groups on the basis of different antigenic determinants they ...

journal_title：Molecular immunology

authors： Nomura M,Imai M,Nakamura T,Miyakawa Y,Mayumi M

更新日期：1982-12-01 00:00:00

abstract：:The T cell stimulatory activity of peptides is known to be associated with the cell surface stability and lifetime of the peptide-MHC (pepMHC) complex. In this report, soluble high-affinity T cell receptors (TCRs) that are specific for pepMHC complexes recognized by the mouse CD8+ clone 2C were used to monitor the cel...

journal_title：Molecular immunology

authors： Brophy SE,Jones LL,Holler PD,Kranz DM

更新日期：2007-03-01 00:00:00

abstract：:An efficient method is described for the isolation of highly purified, IgG-free and stable guinea-pig serum C1q. The procedure includes the chromatography of EDTA-treated serum (25 mM EDTA) on CM- and DEAE-cellulose followed by gel filtration on ACA 34-Ultrogel whereby ammonium sulfate precipitation was used for conce...

journal_title：Molecular immunology

authors： Hitschold T,Golan MD,Rabs U,Loos M

更新日期：1983-02-01 00:00:00

abstract：:A set of 58 overlapping rod-bound peptides was used to map the antigenic reactivity pattern of a 64-residue neurotoxin (AaH II) from the venom of the scorpion Androctonus australis hector. Five anti-toxin rabbit antisera were assayed serially for their capacity to bind to each peptide in the set. Six regions of antige...

journal_title：Molecular immunology

authors： Devaux C,Juin M,Mansuelle P,Granier C

更新日期：1993-08-01 00:00:00

abstract：BACKGROUND:Complement component C5-derived C5a locally generated in the brain has been shown to protect against glutamate-induced neuronal apoptosis and beta-amyloid (Aβ) toxicity, but the mechanism is not clear. In this study, we tested the hypothesis that C5a influences upstream signal transduction pathways associate...

journal_title：Molecular immunology

authors： Gong B,Pan Y,Zhao W,Knable L,Vempati P,Begum S,Ho L,Wang J,Yemul S,Barnum S,Bilski A,Gong BY,Pasinetti GM

更新日期：2013-12-01 00:00:00

abstract：:Based on our findings that HIV-1 gp41 independently of CD4, can bind to the helper T-cell line H9, we characterized putative binding of HIV-1 gp41 to B-cell lines, Raji, Bjab and Ramos. Using fluorescence-activated cell sorter (FACS) we examined the binding of soluble gp41 (sgp41; Env amino acid 539-684) to these B-ce...

journal_title：Molecular immunology

authors： Chen YH,Böck G,Vornhagen R,Steindl F,Katinger H,Dierich MP

更新日期：1993-09-01 00:00:00

abstract：:We have investigated the chemical structure of the antigenic determinant of Candida krusei cell-wall mannan. Acetolysis of the mannan, obtained by extraction with alkali and purified as copper complex, gave five oligosaccharides (from mono- to pentasaccharide) and a minor amount of an octasaccharide. Partial acetolysi...

journal_title：Molecular immunology

authors： Nishikawa A,Shinoda T,Fukazawa Y

更新日期：1982-03-01 00:00:00

abstract：:MAIT cells are innate-like T cells that are enriched in mucosal sites and tissues including adipose tissue and liver. They play an important role in immunity against microbial pathogens. Recently, it has been reported that MAIT cells could also be important in metabolic diseases and can be involved in setting up and m...

journal_title：Molecular immunology

authors： Toubal A,Lehuen A

更新日期：2021-02-01 00:00:00

abstract：:Monoclonal antibodies to cyclosporine A (Cs), a potent immunosuppressant, were generated in BALB/c mice using a novel antigen prepared by linking Cs to a protein carrier via a photoactive cross-linking reagent, 4-benzoylbenzoic acid (BBa). Twenty-two monoclonal anti-Cs antibodies were generated, using Cs-BBa-bovine se...

journal_title：Molecular immunology

authors： Cacalano NA,Aggarwal R,Quesniaux VF,Cleveland WL,Erlanger BF

更新日期：1992-01-01 00:00:00

abstract：:Tumor-associated macrophages (TAMs) are preferentially M2 skewed and promote tumor growth, angiogenesis, invasion, and/or metastasis. In this study, we have analyzed the in vitro immunomodulatory potential of a non-toxic neem leaf glycoprotein (NLGP) in reprogramming Stage III supraglottic laryngeal tumor cell lysate ...

journal_title：Molecular immunology

authors： Goswami KK,Barik S,Sarkar M,Bhowmick A,Biswas J,Bose A,Baral R

更新日期：2014-06-01 00:00:00

abstract：:Calcium signals are crucial for diverse cellular functions including adhesion, differentiation, proliferation, effector functions and gene expression. After engagement of the B cell receptor, the intracellular calcium ion (Ca(2+)) concentration is increased promoting the activation of various signaling cascades. While...

journal_title：Molecular immunology

authors： Baba Y,Matsumoto M,Kurosaki T

更新日期：2014-12-01 00:00:00

abstract：:Immune-mediated glomerular diseases (glomerulonephritis) encompass a heterogeneous collection of diseases that cause inflammation within the glomerulus and other renal compartments with significant morbidity and mortality. In general, CD4+ T cells orchestrate the immune response and play a unique role in autoimmune an...

journal_title：Molecular immunology

authors： Schmidt T,Luebbe J,Paust HJ,Panzer U

更新日期：2018-12-01 00:00:00

abstract：:Antigen-stimulated B lymphocytes undergo genetic and phenotypic changes in germinal centers (GCs), including affinity maturation of immunoglobulin (Ig) genes and Ig heavy chain isotype switching. Expression of the Germinal Center Expressed Transcript (GCET) gene is up-regulated in murine GC B cells. The human homolog ...

journal_title：Molecular immunology

authors： Steinke JW,Hodsdon W,Parenti S,Ostraat R,Lutz R,Borish L,Hagman J

更新日期：2004-11-01 00:00:00

abstract：:The insect gut participates in initial local immune responses by producing reactive oxygen and nitrogen species as well as anti-microbial peptides to resist pathogenic invasions. Nitric oxide (NO), a signaling and an immune effector molecule synthesized by the enzyme NO synthase (NOS), mediates an early step of the si...

journal_title：Molecular immunology

authors： Liu W,Wang Y,Leng Z,Wang Q,Duan X,Luo Y,Jiang Y,Qin L

更新日期：2020-10-01 00:00:00

abstract：:A channel catfish genomic lambda library was screened with CH and JH probes which were derived from our earlier sequence analyses on different full-length heavy chain cDNA clones. One clone, designated C7, contained a genomic insert of about 18 kb and hybridized with specific probes for each of the four domains of the...

journal_title：Molecular immunology

authors： Ghaffari SH,Lobb CJ

更新日期：1992-02-01 00:00:00

abstract：:The cDNA coding for a hybridoma anti Shigella dysenteriae type 1 antibody (3707 E9) has been cloned, and sequenced. Binding patterns with fragments of the bacterial polysaccharide antigen had already been studied in detail. The VH sequence utilizes the VH441 gene, first identified amongst beta-(1,6)galactan-binding an...

journal_title：Molecular immunology

authors： Miller CE,Huppi K,Siwarski D,Karpas A,Newman A,Mainhart C,Glaudemans CP

更新日期：1995-06-01 00:00:00

abstract：:Interleukin-6 (IL-6) is a pleiotropic cytokine that regulates hematopoiesis, inflammation, immune responses and bone homeostasis in mammals. Fish IL-6 has been cloned in recent years but to date no functional studies have been reported. Thus, in this paper we present for the first time in fish the functional character...

journal_title：Molecular immunology

authors： Costa MM,Maehr T,Diaz-Rosales P,Secombes CJ,Wang T

更新日期：2011-09-01 00:00:00

abstract：:In a recent article published in Molecular Immunology [Furukawa, K., Shimizu, T., Murakami, A., Kono, R., Nakagawa, M., Sagawa, T., Yamato, I., Azuma, T., 2007. Strategy for affinity maturation of an antibody with high evolvability to (4-hydroxy-3-nitrophenyl) acetyl hapten. Mol. Immunol. 44, 2436-2445], the authors m...

journal_title：Molecular immunology

authors： Kang J,Warren AS

更新日期：2008-01-01 00:00:00

abstract：:Peptide binding to major histocompatibility complex (MHC) class II molecules is optimal at mildly acidic pH. X-ray crystal structures solved for the murine class II molecule I-E(k) revealed an interesting localization of negatively charged residues within the P6 pocket, which may have implications in the pH dependency...

journal_title：Molecular immunology

authors： Dao CT,Weber DA,Jensen PE

更新日期：2002-06-01 00:00:00

abstract：:Dendritic cells (DCs) are suspected to be involved in the development of atherogenesis, but their role is still unclear. The aim of this study was to characterize circulating DCs and monocyte-derived DCs (Mo-DCs) of obese and diabetic patients (T2D), and to study their interaction with human coronary smooth muscle cel...

journal_title：Molecular immunology

authors： Musilli C,Paccosi S,Pala L,Gerlini G,Ledda F,Mugelli A,Rotella CM,Parenti A

更新日期：2011-10-01 00:00:00

abstract：:Recent studies demonstrate that tissue microenvironments may regulate development and functions of immune cells including dendritic cells, monocytes and T cells. However, there is no report about functional regulation of innate NK cells by tissue microenvironment. The liver is an organ abundant in NK cells and suscept...

journal_title：Molecular immunology

authors： Xia S,Guo Z,Yao Y,Xu X,Yi H,Xia D,Cao X

更新日期：2008-05-01 00:00:00

abstract：:In this study we constructed two phage libraries displaying non-immunized natural human IgM derived HCDR3 repertoires. One library was structurally constrained by a Gly to Cys substitution at position 104 enabling the formation of a disulfide bridge with the Cys at position 92. Panning of these libraries on an anti-hu...

journal_title：Molecular immunology

authors： Deroo S,Fischer A,Beaupain N,Counson M,Boutonnet N,Pletinckx J,Loverix S,Beirnaert E,De Haard H,Schmit JC,Lasters I

更新日期：2008-03-01 00:00:00

abstract：:For decades immunological research has relied, with variable success, on mouse models to investigate diseases and possible therapeutic interventions. With the approval of the first therapeutic antibody targeting complement, called eculizumab, as therapy in paroxysmal nocturnal hemoglobinuria (PNH) and more recently at...

journal_title：Molecular immunology

authors： Pouw RB,Vredevoogd DW,Kuijpers TW,Wouters D

更新日期：2015-09-01 00:00:00

abstract：:The expression and ligand binding activity of Fc receptors for IgG (Fc gamma R) on guinea-pig peripheral blood polymorphonuclear leukocytes (blood PMN) have been compared with those on casein-elicited peritoneal PMN (exudate PMN). Both the PMN were found to express two distinct types of Fc gamma R, one specific for Ig...

journal_title：Molecular immunology

authors： Fukuchi Y,Sato M,Yamashita T,Koyama J

更新日期：1992-05-01 00:00:00

abstract：:Cattle antibodies have unusually long CDR3 loops in their heavy chains (HCs), and limited light chain (LC) diversity, raising the question of whether these mask the effect of LC variation on antigen recognition. We have investigated the role of the LC in the structure and activity of two neutralizing cattle antibodies...

journal_title：Molecular immunology

authors： Ren J,Nettleship JE,Harris G,Mwangi W,Rhaman N,Grant C,Kotecha A,Fry E,Charleston B,Stuart DI,Hammond J,Owens RJ

更新日期：2019-08-01 00:00:00

abstract：:In this paper we report a chemometric approach to Quantitative Structure-Activity Relationship (QSAR) analysis applied to a study of the binding of peptides to Major Histocompatibility Complex (MHC) class I proteins. Peptides which possess the known primary anchor residue motif for HLA-B27 binding do not necessarily b...

journal_title：Molecular immunology

authors： Rovero P,Riganelli D,Fruci D,Viganò S,Pegoŕaro S,Revoltella R,Greco G,Butler R,Clementi S,Tanigaki N

更新日期：1994-05-01 00:00:00

abstract：:Humanization of rodent mAbs by CDR-grafting (also called "reshaping") is now a standard procedure for reducing immunogenicity and recruiting human effector functions. However, the design of the humanized mAb can sometimes prove circuitous. Attempts were made to humanize L-25, a mouse antibody against the human alpha-4...

journal_title：Molecular immunology

authors： Saldanha JW,Martin AC,Léger OJ

更新日期：1999-08-01 00:00:00

abstract：:It is well established that many cell functions are controlled by the PI-3K signaling pathway and the signaling lipid, phosphatidylinositol-3,4,5-triphosphate (PIP3). This is particularly true for mast cells which play a key regulatory role in allergy and inflammation through activation via high-affinity IgE receptors...

journal_title：Molecular immunology

authors： Shenker BJ,Boesze-Battaglia K,Zekavat A,Walker L,Besack D,Ali H

更新日期：2010-11-01 00:00:00

abstract：:When mouse dendritic cells (DCs) are isolated from tissues, purified and placed in a nutritive culture they die more rapidly than would be expected from their normal turnover in vivo. This can distort culture assays of DC function. We therefore tested several approaches to prolonging DC survival in culture. Of several...

journal_title：Molecular immunology

authors： Vremec D,Hansen J,Strasser A,Acha-Orbea H,Zhan Y,O'Keeffe M,Shortman K

更新日期：2015-02-01 00:00:00