Complement in BuB/BnJ mice revisited: serum C3 levels and complement opsonic activity are not elevated.

Abstract:

:With the exception of a few strains such as BuB/BnJ, complement activity in most inbred strains of mice is remarkably similar. The BuB/BnJ strain reportedly has high levels of complement activity and elevated serum levels of C3 and other complement proteins. However, we observed that BuB/BnJ mice have serum C3 levels comparable to those seen in C57BL/6, Balb/c and several other strains of inbred mice. More importantly, using bacteria as a substrate for activation and deposition of complement as a direct biological assay to assess serum complement opsonic activity, we found that BuB/BnJ mice do not have elevated complement activity compared to other inbred mouse strains. In contrast hemolytic assays indicate BuB/BnJ sera to be most active. These results indicate that accepted views regarding mouse serum complement activity need to be re-examined.

journal_name

Mol Immunol

journal_title

Molecular immunology

authors

Osmers I,Szalai AJ,Tenner AJ,Barnum SR

doi

10.1016/j.molimm.2005.10.004

subject

Has Abstract

pub_date

2006-04-01 00:00:00

pages

1722-5

issue

10

eissn

0161-5890

issn

1872-9142

pii

S0161-5890(05)00360-3

journal_volume

43

pub_type

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