Isolation and partial characterization of bovine and equine factor D.

Abstract:

:Bovine and equine factor D were purified to apparent homogeneity as evidenced by a single protein staining band on 7.5-17.5% SDS-PAGE slab gels under both reducing and non-reducing conditions. An apparent mol. wt of 15,000 for bovine D and 22,500 for equine D were noted after SDS-PAGE gel analysis of both reduced and non-reduced preparations. A single polypeptide chain for both proteins was evidenced by the lack of any change in the electrophoretic mobility under each of these conditions. The bovine and equine D were enriched 3347- and 9447-fold, with a 20 and 29% yield of hemolytic activity, respectively. Functionally, both equine and bovine D would reconstitute a human reagent deficient in D (RD), while human or equine D would substitute for bovine D when using a bovine RD. Neither bovine, equine or human D would, however, reconstitute an equine RD.

journal_name

Mol Immunol

journal_title

Molecular immunology

authors

Blanchard DB,Leid RW

doi

10.1016/0161-5890(84)90141-x

subject

Has Abstract

pub_date

1984-10-01 00:00:00

pages

869-76

issue

10

eissn

0161-5890

issn

1872-9142

journal_volume

21

pub_type

杂志文章
  • Identification and expression analysis of two fish-specific IL-6 cytokine family members, the ciliary neurotrophic factor (CNTF)-like and M17 genes, in rainbow trout Oncorhynchus mykiss.

    abstract::The mammalian interleukin (IL)-6 family of cytokines consist of eight class-I helical cytokines and are major players in hematopoiesis, as well as in neuroendocrine and immune systems, and have pro- and anti-inflammatory properties. We have identified a novel fish CNTF-like molecule, for the first time, as well as the...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2009.04.003

    authors: Wang T,Secombes CJ

    更新日期:2009-07-01 00:00:00

  • Toll-like receptor 3 (TLR3) protects retinal pigmented epithelium (RPE) cells from oxidative stress through a STAT3-dependent mechanism.

    abstract::Toll-like receptors (TLRs) are essential receptors of the innate immune system and are first responders for protection against bacterial and viral pathogens. Recently, several TLRs have also been implicated in regulating cell death and survival in non-pathogen injuries such as stroke and oxidative stress. Investigatin...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2012.11.005

    authors: Patel AK,Hackam AS

    更新日期:2013-06-01 00:00:00

  • Non-enzymic activation of the fifth component of human complement, by oxygen radicals. Some properties of the activation product, C5b-like C5.

    abstract::Purified human C5 was converted non-enzymically to an activated form as defined by its ability to participate in reactive lysis. This conversion occurred following exposure to systems that generate oxygen radicals, namely addition of H2O2 in the presence of ascorbic acid and iron or the addition of xanthine oxidase, a...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(89)90057-6

    authors: Vogt W,Damerau B,von Zabern I,Nolte R,Brunahl D

    更新日期:1989-12-01 00:00:00

  • Combined TCRG and TCRA TREC analysis reveals increased peripheral T-lymphocyte but constant intra-thymic proliferative history upon ageing.

    abstract::T-cell receptor (TCR) repertoire diversity, thymic output, clonal size and peripheral T-lymphocyte numbers largely depend on intra-thymic and post-thymic T-lymphocyte proliferation. However, quantitative insight into thymocyte and T-lymphocyte proliferation is still lacking. We developed a new TCRG-based TCR excision ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2012.08.019

    authors: van der Weerd K,Dik WA,Schrijver B,Bogers AJ,Maat AP,van Nederveen FH,van Hagen PM,van Dongen JJ,Langerak AW,Staal FJ

    更新日期:2013-03-01 00:00:00

  • Prolactin peptide (pPRL) induces anti-prolactin antibodies, ROS and cortisol but suppresses specific immune responses in rainbow trout.

    abstract::Prolactin has several immune functions in fish however, the effects on innate and specific components of rainbow trout immunity are currently unknown. Therefore in this study, prolactin peptide (pPRL) injection in rainbow trout generated anti-PRL antibodies that were confirmed through Western blot assays of fish brain...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2020.08.020

    authors: Villalba M,Gómez G,Torres L,Maldonado N,Espiñeira C,Payne G,Vargas-Chacoff L,Figueroa J,Yáñez A,Olavarría VH

    更新日期:2020-11-01 00:00:00

  • An antigenic region of topoisomerase I in DNA polymerase chain reaction-generated fragments recognized by autoantibodies of scleroderma patients.

    abstract::Topoisomerase cDNA and various fragments thereof generated by the DNA polymerase chain reaction were cloned into plasmid expression vectors (pET series) and the expressed polypeptides were probed with scleroderma sera from seven different patients immunoreactive with topoisomerase I. All sera reacted selectively with ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(91)90145-a

    authors: Piccinini G,Cardellini E,Reimer G,Arnett FC,Durban E

    更新日期:1991-04-01 00:00:00

  • Virus-like particle vaccine activates conventional B2 cells and promotes B cell differentiation to IgG2a producing plasma cells.

    abstract::We have previously shown that immunization with SIV-, SHIV-, or HA (influenza hemagglutinin)-virus-like particles (VLPs) elicits a strong humoral immune response in mice. However, little is known about the action VLPs exert on immune effector cells, including B cells. In this study, we found that all three types of VL...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2009.03.008

    authors: Zhang S,Cubas R,Li M,Chen C,Yao Q

    更新日期:2009-06-01 00:00:00

  • Effect of reduction and alkylation on structure and function of rabbit IgG antibody--II. Effects on classical pathway C3 convertase formation.

    abstract::Anaerobic reduction of purified rabbit IgG antibody (Ab) with 1.5 moles of dithiothreitol per mole of Ab at pH 8.0, followed by alkylation, cleaves 39% of the inter-heavy-chain (H-H) disulfide (SS) bonds. This treatment has the following effects on the ability of the Ab to activate the classical pathway of complement....

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(84)90092-0

    authors: Johnson BA,Hoffmann LG

    更新日期:1984-01-01 00:00:00

  • Antigen degradation or presentation by MHC class I molecules via classical and non-classical pathways.

    abstract::Major histocompatibility complex (MHC) class I molecules usually present endogenous peptides at the cell surface. This is the result of a cascade of events involving various dedicated proteins like the peptide transporter associated with antigen processing (TAP) and the ER chaperone tapasin. However, alternative ways ...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审

    doi:10.1016/s0161-5890(02)00101-3

    authors: Grommé M,Neefjes J

    更新日期:2002-10-01 00:00:00

  • Characterization of bovine Toll-like receptor 8: ligand specificity, signaling essential sites and dimerization.

    abstract::Toll-like receptors (TLRs) are a family of highly conserved germline-encoded pattern-recognition receptors (PRRs) and are essential for host immune response. Little is known regarding the activation mechanism of TLRs especially of the TLR7/8/9 subfamily. Here we cloned and characterized bovine TLR8 (bTLR8) and found t...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2008.09.024

    authors: Zhu J,Brownlie R,Liu Q,Babiuk LA,Potter A,Mutwiri GK

    更新日期:2009-02-01 00:00:00

  • NLRX1 attenuates apoptosis and inflammatory responses in myocardial ischemia by inhibiting MAVS-dependent NLRP3 inflammasome activation.

    abstract::Increasing evidence suggests that inflammation and apoptosis are involved in the development of acute myocardial ischemia (AMI). Nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) have recently been identified as key mediators of inflammatory responses. The aim of this study was to explore the speci...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2016.06.013

    authors: Li H,Zhang S,Li F,Qin L

    更新日期:2016-08-01 00:00:00

  • A novel immunoglobulin superfamily junctional molecule expressed by antigen presenting cells, endothelial cells and platelets.

    abstract::The architecture of lymphoid microenvironments depends upon complex interactions between several stromal cell types. We describe in this report the cloning of a cDNA which encodes a novel membrane molecule containing two external Ig-like domains. It is expressed at the junction between endothelial cells including HEV....

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/s0161-5890(98)00102-3

    authors: Malergue F,Galland F,Martin F,Mansuelle P,Aurrand-Lions M,Naquet P

    更新日期:1998-12-01 00:00:00

  • More efficient peptide binding to MHC class II molecules during cathepsin B digestion of Ii than after Ii release.

    abstract::The binding of a T cell-presented peptide to MHC class II alpha,beta chains occurs as a concurrent process with the release of the associated invariant chain (Ii) by cathepsin B. Ii was digested by cathepsin B from solubilized, MHC class II alpha,beta,Ii complexes in the presence of N-hydroxysuccinimidyl-4-azidobenzoa...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(94)90122-8

    authors: Daibata M,Xu M,Humphreys RE,Reyes VE

    更新日期:1994-03-01 00:00:00

  • Immunochemical studies of conjugates of isomaltosyl oligosaccharides to lipid: production and characterization of mouse hybridoma antibodies specific for stearyl-isomaltosyl oligosaccharides.

    abstract::Twelve C57BL/6J hybridoma clones, 9, 2 and 1 from mice immunized with stearyl-isomaltotetraose, stearyl-isomaltopentaose and stearyl-isomaltohexaose respectively were characterized. Seven produced IgA and 5 IgM. The specificities and sizes of their combining sites were determined by quantitative precipitin and precipi...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(85)90105-1

    authors: Lai E,Kabat EA

    更新日期:1985-09-01 00:00:00

  • Molecular characterization of woodchuck CD4 (wCD4) and production of a depletion monoclonal antibody against wCD4.

    abstract::CD4 T cells play an important role in the immune response against hepatitis B virus (HBV) infection. Woodchucks represent an excellent animal model to study HBV infection. In this study, we characterized the cDNA sequence of woodchuck CD4 (wCD4). The deduced wCD4 protein has four extracellular immunoglobulin-like doma...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2013.04.006

    authors: Yang Y,Zhang X,Zhang C,Tao Y,Fan W,Wang Z,Wang H,Lu M,Yang D,Fiedler M,Wang B

    更新日期:2013-11-01 00:00:00

  • Structural analysis of a phosphorylcholine-binding antibody which exhibits a unique carrier specificity for Trichinella spiralis.

    abstract::A phosphorylcholine (PC)-binding IgG (Mab2) antibody produced by a hybridoma derived from a BALB/c mouse which had been immunized against Trichinella spiralis was found to bind to the immunizing antigen (TSC) but not to other PC-associated antigens such as pneumococcal antigen (PNC) and PC-conjugated ovalbumin (PC-OVA...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(94)90106-6

    authors: Lim PL,Leung DT,Chui YL,Ma CH

    更新日期:1994-10-01 00:00:00

  • Human monoclonal antibodies produced in transgenic BABkappa,lambda mice recognising idiotypic immunoglobulins of human lymphoma cells.

    abstract::Clonal idiotypic immunoglobulins of follicular lymphomas can be isolated by somatic fusion procedures. Idiotypic IgMs (Id-IgM) were isolated from two patients and used to immunise a strain of mice, deficient in mouse antibody production and engineered with yeast artificial chromosomes (YAC) containing fragments of the...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2004.03.035

    authors: Suárez E,Yáñez R,Barrios Y,Díaz-Espada F

    更新日期:2004-07-01 00:00:00

  • Identification of the high affinity binding site in the Streptococcus intermedius toxin intermedilysin for its membrane receptor, the human complement regulator CD59.

    abstract::The unique species specificity of the bacterial cytolysin intermedilysin is explained by its requirement for the human complement regulator CD59 as the primary receptor. Binding studies using individual domains of intermedilysin mapped the CD59-binding site to domain 4 and swap mutants between human and rabbit (non-in...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2009.01.003

    authors: Hughes TR,Ross KS,Cowan GJ,Sivasankar B,Harris CL,Mitchell TJ,Morgan BP

    更新日期:2009-04-01 00:00:00

  • Characterisation of Concanavalin A-binding glycoproteins from mouse splenic leukocytes by two-dimensional electrophoresis: preferential binding of incompletely glycosylated forms of H-2 antigen to the lectin.

    abstract::Concanavalin A (Con A)section-binding proteins from mouse spleen leukocytes were characterised by two-dimensional electrophoresis of material precipitated, by Con A plus anti-Con A, from lysates of biosynthetically-labelled cells. Although most cell surface (iodinatable) proteins are known to bind Con A, some of the m...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(83)90030-5

    authors: Pink JR,Hoessli D,Tartakoff A,Hooghe R

    更新日期:1983-04-01 00:00:00

  • Generation of a comprehensive panel of crustacean allergens from the North Sea Shrimp Crangon crangon.

    abstract:BACKGROUND:Published data on crustacean allergens are incomplete. The identification of tropomyosin (TM), arginine kinase (AK), sarcoplasmic Ca-binding protein (SCP) and myosin light chain (MLC) as shrimp allergens are all important contributions but additional allergens are required for the development of a complete s...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2011.06.216

    authors: Bauermeister K,Wangorsch A,Garoffo LP,Reuter A,Conti A,Taylor SL,Lidholm J,Dewitt AM,Enrique E,Vieths S,Holzhauser T,Ballmer-Weber B,Reese G

    更新日期:2011-09-01 00:00:00

  • Autoimmune VH gene family: PCR-generated murine germline VH10 genes.

    abstract::A relatively large number of variable region genes (V) contribute, via gene rearrangements with smaller numbers of additional gene elements (D and J), to generate diversity in the immune response. While some VH gene families are thought to contain 100- 1000 members, the VH10 family has only two known functioning membe...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(92)90032-s

    authors: Chao M,Voss EW Jr

    更新日期:1992-03-01 00:00:00

  • The emerging role of nanomaterials in immunological sensing - a brief review.

    abstract::Nanomaterials are beginning to play an important role in the next generation of immunological assays and biosensors, with potential impacts both in research and clinical practice. In this brief review, we highlight two areas in which nanomaterials are already making new and important contributions in the past 5-10 yea...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审

    doi:10.1016/j.molimm.2017.12.017

    authors: Corrie SR,Plebanski M

    更新日期:2018-06-01 00:00:00

  • Specificity of ganglioside binding to rat macrophages.

    abstract::The binding specificity of rat alveolar macrophages (AM phi) for sheep erythrocytes (E) coated with gangliosides GM1, GM2, GM3, GD1a, GD1b or GT1b was analyzed in a rosette assay by studying the inhibitory effect of gangliosides, various carbohydrates, IgG, C3b-like C3, and fibronectin in this assay. The uptake of gan...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(86)90163-x

    authors: Förster O,Boltz-Nitulescu G,Holzinger C,Wiltschke C,Riedl M,Ortel B,Fellinger A,Bernheimer H

    更新日期:1986-11-01 00:00:00

  • Fever-range hyperthermia inhibits cells immune response to protein-bound polysaccharides derived from Coriolus versicolor extract.

    abstract::The aim of the study was to explore whether fever-range hyperthermia (FRH) might enhance the anticancer and immunoregulatory activities of protein-bound polysaccharides (PBP), a class of fungus derived immunomodifiers used in the cancer adjuvant therapy. Blood lymphocytes and breast cancer cells (MCF-7) were cultured ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2016.10.013

    authors: Pawlikowska M,Jędrzejewski T,Piotrowski J,Kozak W

    更新日期:2016-12-01 00:00:00

  • Effects of sulphated polyanions on functions of complement factor H.

    abstract::Heparin and two dextran sulphate preparations with a low or high average molecular mass (M(r) 5000 and 5 x 10(5), respectively) enhanced binding of radioactively labelled complement factor H to the complement protein C3b, coupled to Sepharose 4B, maximally 2.5-4-fold within a polyanion concn range of 12.5-400 microgra...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(93)90082-m

    authors: Koistinen V

    更新日期:1993-02-01 00:00:00

  • Characterization of Xenopus laevis complement factor I structure--conservation of modular structure except for an unusual insert not present in human factor I.

    abstract::Factor I (C3b/C4b inactivator) is a regulatory protein of the classical and alternative complement pathways. In this paper, we report the sequence of Xenopus factor I cDNA and the deduced protein structure. The basic structure of human preprofactor I, NH2-heavy chain-cleavage peptide-light chain-COOH, is conserved in ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(93)90040-i

    authors: Kunnath-Muglia LM,Chang GH,Sim RB,Day AJ,Ezekowitz RA

    更新日期:1993-10-01 00:00:00

  • The molecular mechanisms of signaling by cooperative assembly formation in innate immunity pathways.

    abstract::The innate immune system is the first line of defense against infection and responses are initiated by pattern recognition receptors (PRRs) that detect pathogen-associated molecular patterns (PAMPs). PRRs also detect endogenous danger-associated molecular patterns (DAMPs) that are released by damaged or dying cells. T...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审

    doi:10.1016/j.molimm.2017.02.012

    authors: Vajjhala PR,Ve T,Bentham A,Stacey KJ,Kobe B

    更新日期:2017-06-01 00:00:00

  • Prevention and control of reciprocal T-B cell diversification: implications for lupus-like autoimmunity.

    abstract::Autoimmunity is fundamentally a continuously evolving process. The autoimmune responses shift, drift and diversify with time not only to other epitopes in the original antigen but also to other related and sometimes to unrelated antigens. We have described a form of immune diversification--reciprocal T-B epitope sprea...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审

    doi:10.1016/j.molimm.2003.11.029

    authors: Singh RR

    更新日期:2004-02-01 00:00:00

  • C1q as an autocrine and paracrine regulator of cellular functions.

    abstract::Most of the complement proteins in circulation are, by and large, synthesized in the liver. However data accumulated over the past several decades provide incontrovertible evidence that some if not most of the individual complement proteins are also synthesized extrahepatically by activated as well as non-activated ce...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审

    doi:10.1016/j.molimm.2016.11.003

    authors: Ghebrehiwet B,Hosszu KH,Peerschke EI

    更新日期:2017-04-01 00:00:00

  • Blocking oncogenic RAS enhances tumour cell surface MHC class I expression but does not alter susceptibility to cytotoxic lymphocytes.

    abstract::Mutations in the RAS family of oncogenes are highly prevalent in human cancer and, amongst its manifold effects, oncogenic RAS impairs the expression of components of the antigen presentation pathway. This allows evasion of cytotoxic T lymphocytes (CTL). CTL and natural killer (NK) cells are reciprocally regulated by ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2013.11.020

    authors: El-Jawhari JJ,El-Sherbiny YM,Scott GB,Morgan RS,Prestwich R,Bowles PA,Blair GE,Tanaka T,Rabbitts TH,Meade JL,Cook GP

    更新日期:2014-04-01 00:00:00