当前位置： SCI文献检索 > MOLECULAR IMMUNOLOGY期刊下所有文献 > Effect of trimming inhibitors on the secretion and biological activity of a murine IgE monoclonal antibody.

Effect of trimming inhibitors on the secretion and biological activity of a murine IgE monoclonal antibody.

Abstract：

:Since secretion of IgE antibodies is known to be blocked by tunicamycin, the first aim of the present study was to determine at which step of glycosylation or processing secretion was restored. For this purpose, murine hybridoma cells secreting an anti beta-lactoglobulin IgE were incubated either in the presence of inhibitors of glucosidase I (castanospermine or N-methyl-1-deoxynojirimycin), or of an inhibitor of Golgi mannosidase II (swainsonine). Terminal galactoses predominate on the native IgE N-linked carbohydrate chains. The action of the trimming inhibitors, which results in changes in these terminal galactose residues, was monitored through detecting binding modifications to Concanavalin A and to the lectin of Ricinus communis. The antibody activity was also evaluated by a radioimmunoassay. It was shown that neither secretion nor anti beta-lactoglobulin activity of the IgE antibody are modified in the presence of any of the trimming inhibitors, whereas secretion is blocked in the presence of tunicamycin. Other biological activities of this IgE were investigated: no difference was observed in the binding of the carbohydrate-modified IgE molecules to normal mouse mast cells, nor to RBL-1 cells, as demonstrated by passive cutaneous anaphylaxis and in vitro binding tests respectively. However, traces of unglycosylated epsilon chain (mol. wt 61,000) found in tunicamycin treated cell supernatant did not bind to RBL-1 cell Fc epsilon receptors. These findings globally suggest that secretion occurs only if the tetradecasaccharide precursor of N-linked carbohydrate chains is transferred from its lipid-carrier to the polypeptide. Further, the presence of such non-processed oligosaccharides (Glc3Man9GlcNAc2) on IgE, does not seem to modify any of the biological activities of this molecule.

journal_name

Mol Immunol

journal_title

Molecular immunology

authors

Granato DA,Neeser JR

doi

10.1016/0161-5890(87)90187-8

subject

Has Abstract

pub_date

1987-08-01 00:00:00

pages

849-55

issue

8

eissn

0161-5890

issn

1872-9142

journal_volume

24

pub_type

杂志文章

相关文献

MOLECULAR IMMUNOLOGY文献大全
  • The allergen Mus m 1.0102: Cysteine residues and molecular allergology.

    abstract：:Mus m 1.0102 is a member of the mouse Major Urinary Protein family, belonging to the Lipocalins superfamily. Major Urinary Proteins (MUPs) are characterized by highly conserved structural motifs. These include a disulphide bond, involved in protein oxidative folding and protein structure stabilization, and a free cyst...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2020.01.022

    authors： Ferrari E,Corsini R,Burastero SE,Tanfani F,Spisni A

    更新日期：2020-04-01 00:00:00

  • PECAM-1-regulated signalling thresholds control tolerance in anergic transgenic B-cells.

    abstract：:Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1/CD31) is an immunoglobulin (Ig)-immunoreceptor tyrosine based inhibitory motif (Ig-ITIM) superfamily member that recruits and activates protein-tyrosine phosphatases, predominantly SHP-2 and to a lesser extent, SHP-1. Previously, we have shown that deletion of PEC...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2007.09.024

    authors： Wong MX,Hayball JD,Jackson DE

    更新日期：2008-03-01 00:00:00

  • Interaction of C3 and C3b with immunoglobulin G.

    abstract：:Human C3b as well as native C3 were found to bind to solid phase human and rabbit IgG. Haemolytically active C3 had significantly higher binding capacity to IgG than the C3b fragment. Inhibition experiments proved that C3 and C3b have common binding sites on the Fab and Fc part of the IgG molecule but the character of...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(83)90076-7

    authors： Kulics J,Rajnavölgyi E,Füst G,Gergely J

    更新日期：1983-08-01 00:00:00

  • Role of MAIT cells in metabolic diseases.

    abstract：:MAIT cells are innate-like T cells that are enriched in mucosal sites and tissues including adipose tissue and liver. They play an important role in immunity against microbial pathogens. Recently, it has been reported that MAIT cells could also be important in metabolic diseases and can be involved in setting up and m...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2020.12.014

    authors： Toubal A,Lehuen A

    更新日期：2021-02-01 00:00:00

  • An afucosylated anti-CD20 monoclonal antibody with greater antibody-dependent cellular cytotoxicity and B-cell depletion and lower complement-dependent cytotoxicity than rituximab.

    abstract：:The objective of this study was to characterize the in vitro and in vivo activity of a novel afucosylated rituximab (BLX-300) expressed in a Lemna aquatic plant-based system free of zoonotic pathogens. The glycosylation of BLX-300 was shown to be homogeneous, composed of a single major N-glycan species without detecta...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2012.01.001

    authors： Gasdaska JR,Sherwood S,Regan JT,Dickey LF

    更新日期：2012-03-01 00:00:00

  • Evaluation of the role of the 3'alpha heavy chain enhancer [3'alpha E(hs1,2)] in Vh gene somatic hypermutation.

    abstract：:Previous work on the cis-acting elements that control heavy chain variable region (VH) gene somatic hypermutation has indicated the presence of an as yet unidentified element(s) 3' of the intron enhancer that is necessary for high rate mutation. Examination of cis-acting elements involved in kappa light chain V gene h...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/s0161-5890(97)00065-5

    authors： Tumas-Brundage KM,Vora KA,Manser T

    更新日期：1997-04-01 00:00:00

  • MLKL deficiency inhibits DSS-induced colitis independent of intestinal microbiota.

    abstract：:The maintenance of intestinal tissue homeostasis is vital for the resistance against inflammatory bowel diseases (IBDs). Necroptosis is identified as an alternative mode of regulated cell death, which plays a pivotal role in tissue homeostasis. Thus, the roles of RIP3-mediated necroptosis in intestinal inflammation ha...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2019.01.018

    authors： Zhang J,Qin D,Yang YJ,Hu GQ,Qin XX,Du CT,Chen W

    更新日期：2019-03-01 00:00:00

  • The heterogeneity of bovine IgG2--VIII. The complete cDNA sequence of bovine IgG2a (A2) and an IgG1.

    abstract：:The complete cDNA sequences of two bovine IgGs were obtained by RT-PCR cloning. The first-strand cDNA was prepared from an animal homozygous (A2/A2) for IgG2a; the resulting sequences of the two bovine IgGs reported here were identified as IgG2a(A2) and IgG1. These sequences, and their deduced amino acid sequences, ar...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(95)00107-7

    authors： Kacskovics I,Butler JE

    更新日期：1996-02-01 00:00:00

  • Regulation of interleukin-2 receptor expression and receptor release.

    abstract：:The generation and cell surface expression of IL-2 receptors was monitored by: (i) an ELISA that permits quantitative determination of detergent-solubilized or soluble IL-2 receptors; and (ii) detection of the binding of 125I-labelled recombinant IL-2 and of anti-IL-2 receptor antibodies to receptor bearing cells. Upo...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(86)90147-1

    authors： Diamantstein T,Osawa H,Mouzaki A,Josimovic-Alasevic O

    更新日期：1986-11-01 00:00:00

  • IgE epitopes of intact and digested Ara h 1: a comparative study in humans and rats.

    abstract：BACKGROUND:Allergen epitope characterization provides valuable information useful for the understanding of proteins as food allergens. It is believed that IgE epitopes in general are conformational, nevertheless, for food allergens known to sensitize through the gastrointestinal tract linear epitopes have been suggeste...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2012.04.002

    authors： Bøgh KL,Nielsen H,Madsen CB,Mills EN,Rigby N,Eiwegger T,Szépfalusi Z,Roggen EL

    更新日期：2012-07-01 00:00:00

  • Evidence that negative feedback between antibody concentration and affinity regulates humoral response consolidation to a non-infectious antigen in infants.

    abstract：:The dynamics of human antigen-specific immunoglobulin (Ig) responses in early life are not well characterized. We have previously observed an inverse relationship between allergen-specific Ig concentration and allergen-Ig-binding affinity in allergen-sensitive atopic adults, suggesting a possible feedback relationship...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2004.07.006

    authors： Jackola DR,Liebeler CL,Lin CY,Chiu YK,Blumenthal MN,Rosenberg A

    更新日期：2005-01-01 00:00:00

  • Lactobacillus casei suppresses experimental arthritis by down-regulating T helper 1 effector functions.

    abstract：:Although the beneficial effects of probiotics on wide variety of diseases have been shown, little is known about how probiotics modulate the immune system. In this study we elucidated the underlying mechanisms how Lactobacillus casei (L. casei) protects against rheumatoid arthritis (RA) progression by investigating th...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2007.12.010

    authors： So JS,Kwon HK,Lee CG,Yi HJ,Park JA,Lim SY,Hwang KC,Jeon YH,Im SH

    更新日期：2008-05-01 00:00:00

  • Nitric oxide plays a crucial role in midgut immunity under microsporidian infection in Antheraea pernyi.

    abstract：:The insect gut participates in initial local immune responses by producing reactive oxygen and nitrogen species as well as anti-microbial peptides to resist pathogenic invasions. Nitric oxide (NO), a signaling and an immune effector molecule synthesized by the enzyme NO synthase (NOS), mediates an early step of the si...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2020.07.018

    authors： Liu W,Wang Y,Leng Z,Wang Q,Duan X,Luo Y,Jiang Y,Qin L

    更新日期：2020-10-01 00:00:00

  • Characterisation of Concanavalin A-binding glycoproteins from mouse splenic leukocytes by two-dimensional electrophoresis: preferential binding of incompletely glycosylated forms of H-2 antigen to the lectin.

    abstract：:Concanavalin A (Con A)section-binding proteins from mouse spleen leukocytes were characterised by two-dimensional electrophoresis of material precipitated, by Con A plus anti-Con A, from lysates of biosynthetically-labelled cells. Although most cell surface (iodinatable) proteins are known to bind Con A, some of the m...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(83)90030-5

    authors： Pink JR,Hoessli D,Tartakoff A,Hooghe R

    更新日期：1983-04-01 00:00:00

  • Cloning of a crustin-like, single whey-acidic-domain, antibacterial peptide from the haemocytes of the European lobster, Homarus gammarus, and its response to infection with bacteria.

    abstract：:Degenerate PCR was used to isolate a 221-base pair nucleotide sequence of a new crustin-like antibacterial peptide from the haemocytes of the European lobster, Homarus gammarus. Rapid amplification of cDNA ends was used to extend the sequence to determine the complete open reading frame and un-translated regions. The ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2005.07.029

    authors： Hauton C,Brockton V,Smith VJ

    更新日期：2006-03-01 00:00:00

  • Transcriptome response following administration of a live bacterial vaccine in Atlantic salmon (Salmo salar).

    abstract：:Antibacterial responses have been studied in Atlantic salmon following an acute intra peritoneal injection of a genetically attenuated (aroA(-)) strain of Aeromonas salmonicida known to elicit protective immunity. Three tissues were studied for transcriptional changes, the liver, head kidney and the gill. RNA was coll...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2005.10.007

    authors： Martin SA,Blaney SC,Houlihan DF,Secombes CJ

    更新日期：2006-04-01 00:00:00

  • Exogenous interleukin-2 does not reverse the immunoinhibitory effects of 1,25-dihydroxyvitamin D3 on human peripheral blood lymphocyte immunoglobulin production.

    abstract：:1,25-Dihydroxyvitamin D3 (1,25-D3) is known to have potent inhibitory effects on human peripheral blood mononuclear cell (PBMC) functions. Previous experiments suggest that addition of interleukin-2 (IL-2) to cell cultures can reverse the antiproliferative action of 1,25-D3. Previous studies have also shown that the C...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(90)90064-7

    authors： Jordan SC,Lemire JM,Sakai RS,Toyoda M,Adams JS

    更新日期：1990-01-01 00:00:00

  • C-mip interacts physically with RelA and inhibits nuclear factor kappa B activity.

    abstract：:The fine regulation of NF-kappaB activity is crucial for both resting and stimulated cells and relies on complex balance between multiple activators and inhibitors. We report here that c-mip, a recently identified pleckstrin homology (PH) and leucine-rich repeat (LRR)-domain-containing protein, inactivates GSKbeta and...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2008.09.034

    authors： Kamal M,Valanciute A,Dahan K,Ory V,Pawlak A,Lang P,Guellaen G,Sahali D

    更新日期：2009-02-01 00:00:00

  • Circular Dichroism reveals evidence of coupling between immunoglobulin constant and variable region secondary structure.

    abstract：:Antibodies (Ab) are bifunctional molecules with two domains, a constant region (C) that confers effector properties and a variable (V) region responsible of antigen (Ag) binding. Historically the C and V regions were considered to be functionally independent, with Ag specificity being solely determined by the V region...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2010.02.018

    authors： Janda A,Casadevall A

    更新日期：2010-04-01 00:00:00

  • Molecular characterization of the dimer formation of Fcα/μ receptor (CD351).

    abstract：:Fcα/μR (CD351) is an Fc receptor for both IgA and IgM and forms an atypical dimer that is resistant to reduction by 2-mercaptoethanol or boiling. We previously demonstrated that the cytoplasmic portion of Fcα/μR is required for dimer formation and for its efficient cell-surface expression. However, the biochemical nat...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2013.04.003

    authors： Takagaki K,Satoh K,Honda S,Shibuya A

    更新日期：2013-11-01 00:00:00

  • Peanut lectin and anti-Ii antibodies reveal structural differences among human gastrointestinal glycoproteins.

    abstract：:Human gastrointestinal glycoproteins (mucins), isolated by pepsin digestion from foetal stomachs and meconia, and from paired tumour and non-neoplastic mucosal samples of patients with gastric and colorectal carcinomas, were tested for precipitating reactions with peanut lectin (PNL) and four anti-carbohydrate antibod...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(83)90145-1

    authors： Picard JK,Feizi T

    更新日期：1983-11-01 00:00:00

  • Cloning, expression analysis and functional characterization of an interleukin-1 receptor-associated kinase 4 from Apostichopus japonicus.

    abstract：:Interleukin 1 receptor-associated kinase 4 (IRAK4) is a key factor in TLR-mediated host immune function. In this study, an IRAK4 homologue molecule was identified from Apostichopus japonicus (designated as AjIRAK4) by RACE approach. The full-length cDNA of AjIRAK4 was of 2024 bp containing an open reading frame of 131...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2018.08.006

    authors： Cui Y,Jiang L,Xing R,Wang Z,Wang Z,Shao Y,Zhang W,Zhao X,Li C

    更新日期：2018-09-01 00:00:00

  • Evaluation of structure-antigenicity relationship of peptides from human immunodeficiency virus type 1 (HIV-1) p18 protein by circular dichroism.

    abstract：:The antigenicity of Human Immunodeficiency Virus type 1 (HIV-1) matrix p18 protein was evaluated by analyzing the specificity of anti-p18 antibodies elicited either in HIV-1 infected humans, or in HIV-1 infected or immunized chimpanzees, against a panel of long and short overlapping synthetic peptides [from 12 to 46 a...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(93)90118-u

    authors： Mabrouk K,Moulard M,Gluckman JC,Romi R,Rochat H,Van Rietschoten J,Bahraoui E

    更新日期：1993-04-01 00:00:00

  • Comparative research on nucleocapsid and spike glycoprotein as the rapid immunodetection targets of COVID-19 and establishment of immunoassay strips.

    abstract：:The SARS-CoV-2 virus responsible for coronavirus 2019 (COVID-19) poses a significant challenge to healthcare systems worldwide. According to the World Health Organization (WHO), the outbreak of COVID-19 has been a pandemic that infected more than 25.32 million people and caused more than 848.25 thousand deaths worldwi...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2021.01.005

    authors： Liu D,Wu F,Cen Y,Ye L,Shi X,Huang Y,Fang S,Ma L

    更新日期：2021-01-09 00:00:00

  • Effects of eosinophils on mast cells: a new pathway for the perpetuation of allergic inflammation.

    abstract：:Mast cells have a clear-cut pathologic role in allergy, participating in a number of chronic inflammatory conditions, in helmintic parasitosis, and in some solid tumor reactions, but also in physiological situations, such as wound healing and innate immunity. Mast cells release a large number of proinflammatory, immun...

    journal_title：Molecular immunology

    pub_type： 杂志文章,评审

    doi：10.1016/s0161-5890(02)00090-1

    authors： Piliponsky AM,Gleich GJ,Bar I,Levi-Schaffer F

    更新日期：2002-09-01 00:00:00

  • Molecular comparison of mouse Thy-1 and its human homologue.

    abstract：:Physicochemical techniques were used to verify previous immunochemical studies showing homology of the human Thy-1 (formerly p25) antigen and the murine Thy-1 or theta antigen. Peptide maps and amino acid compositions showed close similarity between these two proteins; however, they were not identical. These data conf...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(82)90216-4

    authors： Bonewald L,Wang AC,Terry E,Zwerner R,Balch CM,Ades EW

    更新日期：1982-03-01 00:00:00

  • Active phagocytosis of Mycobacterium tuberculosis (H37Ra) by T lymphocytes (Jurkat cells).

    abstract：:This study aimed to co-culture Jurkat T lymphocytes with inactivated Mycobacterium tuberculosis (Mtb H37Ra), explore whether T lymphocytes could phagocytose H37Ra cells, and determine the underlying mechanism. Jurkat T lymphocytes were co-cultured with H37Ra cells, and confocal laser scanning microscopy, electron micr...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2015.04.018

    authors： Zhang M,Zhu Q,Shi M,Liu Y,Ma L,Yang Y,Feng D,Dai W,Zhang L,Kang T,Chen P,He Y,Liu T,Zhao Q,Wang W,Zhi J,Feng G,Zhao G

    更新日期：2015-08-01 00:00:00

  • Comparison of vasoactive intestinal peptide-mediated protein phosphorylation in human lymphoblasts and colonic epithelial cells.

    abstract：:Vasoactive intestinal peptide (VIP) induces phosphorylation of a basic 38,000 mol. wt protein in a human lymphoblastic cell line (Molt 4b) and a human colon carcinoma cell line (HT29). In both cell types, VIP interacts with specific high affinity receptors to activate adenylate cyclase and cAMP-dependent protein kinas...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(89)90010-2

    authors： O'Dorisio MS,Campolito LB

    更新日期：1989-06-01 00:00:00

  • The biochemistry and immunology of non-canonical forms of HLA-B27.

    abstract：:HLA-B27 (B27) is strongly associated with the spondyloarthritides. B27 is expressed at the cell surface of antigen presenting cells (APC) both as canonical β2m-associated and non-canonical β2m-free heavy chain (FHC) forms which include B27 dimers (termed B272). B27 FHC forms arise in an endosomal compartment from recy...

    journal_title：Molecular immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.molimm.2013.05.243

    authors： Shaw J,Hatano H,Kollnberger S

    更新日期：2014-01-01 00:00:00

  • C1q binding by a high affinity anti-fluorescein murine monoclonal IgM antibody and monomeric subunits.

    abstract：:Comparative interactions of purified rabbit C1q with 18-2-3, a high affinity (2-3 X 10(10) M-1) anti-fluorescein (anti-F1) murine monoclonal IgM antibody (pentamer) and constitutive monomeric subunits (IgMs) were studied. Using a solid phase radioimmunoassay (SPRIA), based on immobilized polyvalent antigen, it was sho...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(88)90076-4

    authors： Swanson SM,Dombrink-Kurtzman MA,Voss EW Jr

    更新日期：1988-06-01 00:00:00