Abstract：
:The capsid protein of hepatitis B virus (P19) is made of 183 amino acids and carries the antigenic sites of hepatitis B core antigen (HBcAg) and hepatitis B e antigen (HBeAg) on the amino-terminal domain. The carboxyl-terminal domain of P19 (amino acids 150-183) is arginine-rich (47%) and faces the interior of the nucleocapsid for the binding with DNA. Monoclonal antibody was raised against an antigenic site on this protamine-like region of P19, which was distinct from HBcAg or HBeAg sites, and the novel antigenic site(s) was provisionally designated as hepatitis B inner core antigen (HBicAg). When P19 in a low concn (150 ng/ml) was immobilized on the solid surface, HBicAg sites were preserved, while HBcAg or HBcAg sites were no longer available on it. This allowed the detection of antibodies against HBicAg (anti-HBic), by sandwiching them between immobilized P19 and anti-IgG labeled with horseradish peroxidase. Anti-HBic was detected in sera from HBsAg carriers, typically those seropositive for antibody to HBeAg. A synthetic arginine-rich decapeptide, with a sequence of Arg-Arg-Arg-Gly-Arg-Ser-Pro-Arg-Arg-Arg, representing amino acids 150-159 of P19 and conserved in the majority of reported hepatitis B virus, absorbed the activity to bind with P19 in seven (44%) out of 16 sera containing anti-HBic. These results indicate that the decapeptide carries an HBicAg epitope and the remaining amino acid sequence of the arginine-rich carboxyl terminal domain (160-183) may be responsible for the other HBicAg epitopes.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Machida A,Ohnuma H,Takai E,Tsuda F,Tanaka T,Naito M,Munekata E,Miyakawa Y,Mayumi Mdoi
10.1016/0161-5890(89)90130-2subject
Has Abstractpub_date
1989-04-01 00:00:00pages
413-21issue
4eissn
0161-5890issn
1872-9142journal_volume
26pub_type
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更新日期：1982-03-01 00:00:00
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更新日期：2007-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：1995-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：1990-03-01 00:00:00
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