The immune response to Epstein-Barr nuclear antigen: conformational and structural features of antibody binding to synthetic peptides.

abstract：:The cell-surface receptor ST2L triggers cytokine release by immune cells upon exposure to its ligand IL-33. To study the effect of ST2L-dependent signaling in different cell types, we generated antagonist antibodies that bind different receptor domains. We sought to characterize their activities in vitro using both tr...

journal_title：Molecular immunology

authors： Fursov N,Lu J,Healy C,Wu SJ,Lacy E,Filer A,Li Y,Liu C,Lamb R,Jones B,Reddy R,Petley T,Duffy K

更新日期：2016-07-01 00:00:00

abstract：:Mammals have evolved a special cellular mechanism for killing invading microbes, which is called the phagocytosis. Neutrophils are the first phagocytosing cells that migrate into the site of infection. In these cells, hypochlorite (HOCl) and other hypohalites, generated in the myeloperoxidase (MPO)-hydrogen peroxide (...

journal_title：Molecular immunology

authors： Atosuo J,Suominen E

更新日期：2019-12-01 00:00:00

abstract：:Candida albicans is an opportunistic human-pathogenic fungus, which can cause superficial but also life-threatening invasive infections. The pH-regulated antigen 1 (Pra1) of C. albicans is a surface-associated and secreted protein highly expressed in the hyphal form. Pra1 can bind to complement receptor 3 (CD11b/CD18)...

journal_title：Molecular immunology

authors： Losse J,Svobodová E,Heyken A,Hube B,Zipfel PF,Józsi M

更新日期：2011-09-01 00:00:00

abstract：:We examined the expression of T15 idiotopes (Id) on phosphorylcholine (PC)-binding monoclonal immunoglobulins and defined the structural correlates of these Id. The seven monoclonal anti-Id antibodies used as probes recognize distinct determinants that range from the antigen binding site to the CH1 domain on TEPC15. C...

journal_title：Molecular immunology

authors： Strickland FM,Gleason JT,Cerny J

更新日期：1987-06-01 00:00:00

abstract：:N-Acetylcysteine (NAC) has been widely used as an antioxidant in research, however, it has also been found to reduce the binding of TNF to its receptor independent of its antioxidative role. In this study, we investigated the effect of NAC on NF-kappaB activation. In HeLa cells, Hep3B cells, and A549 cells, DNA-bindin...

journal_title：Molecular immunology

authors： Liu J,Yoshida Y,Yamashita U

更新日期：2008-09-01 00:00:00

abstract：:Apoptosis can be induced in the human leukemic T-cell line Jurkat when these cells are stimulated by several known T-cell activators. Among the stimuli that activate Jurkat cells, calcium ionophore A23187, phytohemagglutinin and cross-linked monoclonal antibody anti-CD3 are the best inducers of apoptosis. The activato...

journal_title：Molecular immunology

authors： Ruiz-Ruiz MC,Oliver FJ,Izquierdo M,López-Rivas A

更新日期：1995-09-01 00:00:00

abstract：:This study describes serological and biochemical properties of a novel MHC class I molecule. The mutant H-2Ksm1 molecule was discovered in a mouse because of loss of reactivity of its peripheral blood lymphocytes to monoclonal antibodies. This mutation in the H-2Ks molecule is the first in vivo mutation described that...

journal_title：Molecular immunology

authors： Hasenkrug KJ,Kesari KV,Nathenson SG

更新日期：1992-12-01 00:00:00

abstract：:The cancer immunosurveillance hypothesis has found strong experimental support in recent years. It is believed that cytotoxic lymphocytes are important effectors in this process. PKCtheta plays an essential role in proliferation, activation and survival of these cells, but also proliferation and survival of leukemic T...

journal_title：Molecular immunology

authors： Garaude J,Kaminski S,Charni S,Aguilò JI,Jacquet C,Plays M,Hernandez J,Rodriguez F,Hipskind RA,Anel A,Villalba M

更新日期：2008-07-01 00:00:00

abstract：:Previously we reported that, early in the antibody response of BALB/c mice to several cytochromes c (CYT) coupled to ovalbumin (OVA), B cells responding to the self antigen mouse CYT recognized a single site on mouse CYT and were in much higher frequency than B cells responding to foreign CYT. In the present study the...

journal_title：Molecular immunology

authors： Mueller CM,Minnerath JM,Jemmerson R

更新日期：1997-08-01 00:00:00

abstract：:To test the fate of developing B cells with autoreactive receptor components, we studied mice homozygous for a knock-in transgene coding the VH domain of an IgM ssDNA-binding antibody. The transgene has unmutated C57 BL/6 V gene segments. Homozygous knock-in mice developed normal numbers of spleen and bone marrow B ce...

journal_title：Molecular immunology

authors： Li J,Geissal ED,Li W,Stollar BD

更新日期：2005-08-01 00:00:00

abstract：:Mouse monoclonal antibody AUK12-20 binds to human IL-6 receptor and inhibits IL-6 functions. It has been humanized by CDR-grafting for therapeutic use. In the design of reshaped human AUK12-20 VL region, the human framework regions (FRs) from the human Bence-Jones protein REI were used. The reshaped human AUK12-20 lig...

journal_title：Molecular immunology

authors： Sato K,Tsuchiya M,Saldanha J,Koishihara Y,Ohsugi Y,Kishimoto T,Bendig MM

更新日期：1994-04-01 00:00:00

abstract：:The structural characteristics of antigens recognized by a panel of monoclonal antibodies prepared against a rabbit T-lymphocyte cell line have been investigated. Those antigens which could be isolated using immunoadsorbents prepared from the monoclonal antibodies had mol. wts of 42,000, 90,000 and 120,000. The 42,000...

journal_title：Molecular immunology

authors： Wilkinson JM,Wetterskog DL,Sogn JA,Kindt TJ

更新日期：1984-01-01 00:00:00

abstract：:Bothropasin is a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops jararaca venom, the snake responsible for most bites in Southeastern Brazil. SVMPs, such as bothropasin, are involved in the main bothropic envenoming symptoms, which include hemorrhage, inflammation, necrosis and blood coagulation deficie...

journal_title：Molecular immunology

authors： Molina Molina DA,Guerra-Duarte C,Naves de Souza DL,Costal-Oliveira F,Ávila GR,Soccol VT,Machado-de-Ávila RA,Chávez-Olórtegui C

更新日期：2018-12-01 00:00:00

abstract：:Humanization of rodent mAbs by CDR-grafting (also called "reshaping") is now a standard procedure for reducing immunogenicity and recruiting human effector functions. However, the design of the humanized mAb can sometimes prove circuitous. Attempts were made to humanize L-25, a mouse antibody against the human alpha-4...

journal_title：Molecular immunology

authors： Saldanha JW,Martin AC,Léger OJ

更新日期：1999-08-01 00:00:00

abstract：:Pathogenic fungi represent a major threat particularly to immunocompromised hosts, leading to severe, and often lethal, systemic opportunistic infections. Although the impaired immune status of the host is clearly the most important factor leading to disease, virulence factors of the fungus also play a role. Factor H ...

journal_title：Molecular immunology

authors： Vogl G,Lesiak I,Jensen DB,Perkhofer S,Eck R,Speth C,Lass-Flörl C,Zipfel PF,Blom AM,Dierich MP,Würzner R

更新日期：2008-03-01 00:00:00

abstract：:The p38 mitogen-activated protein kinases (MAPKs) are essential cytoplasmic signal molecules of innate immune pathways that play a vital role in host immune defense responses to pathogenic challenges. In this study, two fish p38 genes (Cip38α and Cip38β) were characterized for the first time from the grass carp Ctenop...

journal_title：Molecular immunology

authors： Sun Y,Xu W,Li D,Zhou H,Qu F,Cao S,Tang J,Zhou Y,He Z,Li H,Zhou Z,Liu Z

更新日期：2020-02-01 00:00:00

abstract：:Lysozyme is an important component of the insect non-specific immune response against bacteria that is characterized by its ability to break down bacterial cell-walls. By searching an EST database from the fall armyworm, Spodoptera frugiperda (Negre et al., 2006), we identified five sequences encoding proteins of the ...

journal_title：Molecular immunology

authors： Chapelle M,Girard PA,Cousserans F,Volkoff NA,Duvic B

更新日期：2009-12-01 00:00:00

abstract：:Mice deficient for SPI-group ETS transcription factors PU.1 or SPI-B fail to generate lymphocytes or do not mount normal antibody mediated immune responses, respectively. PU.1 expression is restricted to B-, T-lymphocytes and macrophages, while SPI-B is expressed in B- and T-lymphocytes. SPI-C is an ETS transcription ...

journal_title：Molecular immunology

authors： Carlsson R,Persson C,Leanderson T

更新日期：2003-06-01 00:00:00

abstract：:Extracts of blood lymphocytes, polymorphonuclear neutrophils and B, T or monocytic cell lines were analyzed by two-dimensional gel electrophoresis and immunoradiometric assay after electro-transfer to nitrocellulose sheets with radiolabelled polyclonal or monoclonal antibodies specific for beta 2-microglobulin. Four d...

journal_title：Molecular immunology

authors： Vincent C,Ramackers JM,Bonnefoy N,Revillard JP

更新日期：1989-08-01 00:00:00

abstract：:Many infections are associated with diabetes, as the ability of the body to fight pathogens is impaired. Recently, altered levels of defensins and polymorphism in Toll-like receptors (TLRs), two crucial components of the innate immune system, have been associated with diabetes. To study the functionality of the innate...

journal_title：Molecular immunology

authors： Froy O,Hananel A,Chapnik N,Madar Z

更新日期：2007-02-01 00:00:00

abstract：:A porcine cDNA library was constructed using poly(A)+ RNA isolated from the spleen of an adult Minnesota miniature swine. Screening the library with antisera specific for porcine immunoglobulin light chains resulted in the selection and isolation of two recombinant clones, PLC18 and PLC3, which encode for kappa and la...

journal_title：Molecular immunology

authors： Lammers BM,Beaman KD,Kim YB

更新日期：1991-08-01 00:00:00

abstract：:The human C epsilon gene produces a number of alternatively spliced heavy chain transcripts of which some encode functional IgE isoforms. We now show that differentially processed epsilon mRNA variants also exist in the mouse and are generated by differential polyadenylation and alternative splicing of primary epsilon...

journal_title：Molecular immunology

authors： Anand S,Batista FD,Tkach T,Efremov DG,Burrone OR

更新日期：1997-02-01 00:00:00

abstract：:Arginine kinase (AK) is a panallergen present in crustaceans, which can induce an immunoglobulin (Ig) E-mediated immune response in humans. The aim of this work was to map and characterize the antigenic epitopes of Scylla paramamosain AK. Specific-protein-A-enriched IgG raised in rabbits against purified S. paramamosa...

journal_title：Molecular immunology

authors： Yang Y,Cao MJ,Alcocer M,Liu QM,Fei DX,Mao HY,Liu GM

更新日期：2015-06-01 00:00:00

abstract：:In this paper, we performed further biochemical characterization of the CD26 antigen, as defined by the mAbs in anti-1F7 and anti-Ta1, in order to clarify the observed functional differences among these mAbs. For this purpose, we developed a mAb, anti-5F8, which recognizes yet another epitope on the CD26 antigen diffe...

journal_title：Molecular immunology

authors： Torimoto Y,Dang NH,Tanaka T,Prado C,Schlossman SF,Morimoto C

更新日期：1992-02-01 00:00:00

abstract：:Histocompatibility Leukocyte Antigens, or HLAs, are one of the most polymorphic molecules in humans. This high degree of polymorphism endows HLA molecules with the ability to present a vast array of peptides, an essential trait for responding to ever-evolving pathogens. Unlike classical HLA molecules (HLA-Ia), some no...

journal_title：Molecular immunology

authors： Grant EJ,Nguyen AT,Lobos CA,Szeto C,Chatzileontiadou DSM,Gras S

更新日期：2020-04-01 00:00:00

abstract：:Dendritic cells (DCs) serve to maintain peripheral tolerance under steady state conditions. Upon triggering by activation signals they initiate strong immune responses. The activation of DCs is accompanied by a rapid upregulation of proinflammatory cytokines, which were shown in other cell types to be regulated by mec...

journal_title：Molecular immunology

authors： Bros M,Wiechmann N,Besche V,Art J,Pautz A,Grabbe S,Kleinert H,Reske-Kunz AB

更新日期：2010-02-01 00:00:00

abstract：OBJECTIVE:To investigate the impact of death-associated protein kinase 1 (Dapk1) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) via p38MAPK/NF-κB pathway. METHODS:Dapk1+/+ and Dapk1-/- mice were randomized into Control, LPS, SB203580 (a p38MAPK pathway inhibitor) + LPS, and PDTC (a NF-κB pathway inhibitor...

journal_title：Molecular immunology

authors： Li T,Wu YN,Wang H,Ma JY,Zhai SS,Duan J

更新日期：2020-04-01 00:00:00

abstract：:The development and progression of systemic lupus erythematosus (SLE) is strongly associated with complement activation and deposition. The anaphylatoxin C3a is a product of complement activation with immunomodulatory properties, and the receptor for C3a (C3aR) is not only expressed by granulocytes and antigen present...

journal_title：Molecular immunology

authors： Wenderfer SE,Wang H,Ke B,Wetsel RA,Braun MC

更新日期：2009-04-01 00:00:00

abstract：:CD1 molecules are MHC-unlinked class Ib molecules consisting of classical (human CD 1a-c) and non-classical subsets (human CD1d and murine CD1). The characterization of non-classical subsets of CD1 is limited due to the lack of reagents. In this study, we have generated two new anti-mouse CD1 monoclonal antibodies, 3H...

journal_title：Molecular immunology

authors： Mandal M,Chen XR,Alegre ML,Chiu NM,Chen YH,Castaño AR,Wang CR

更新日期：1998-06-01 00:00:00

abstract：:Although the beneficial effects of probiotics on wide variety of diseases have been shown, little is known about how probiotics modulate the immune system. In this study we elucidated the underlying mechanisms how Lactobacillus casei (L. casei) protects against rheumatoid arthritis (RA) progression by investigating th...

journal_title：Molecular immunology

authors： So JS,Kwon HK,Lee CG,Yi HJ,Park JA,Lim SY,Hwang KC,Jeon YH,Im SH

更新日期：2008-05-01 00:00:00