Abstract：
:The integral membrane protein flavocytochrome b (Cyt b) is the catalytic core of the NADPH oxidase complex, a multicomponent enzyme system that initiates a cascade of reactive oxygen species that play a critical role in innate immunity and vascular physiology. Epitope-mapped, monoclonal antibodies (mAb) that recognize the large (gp91phox) and small (p22phox) subunits of Cyt b provide valuable reagents that have been used to examine structural and mechanistic aspects of oxidase function. In the present study, the heavy and light chain variable region genes of the Cyt b-specific mAbs 44.1, NS5, and NL7 have been amplified by RT-PCR, cloned and subject to DNA sequence analysis. Since the 5' degenerate primer sets used for mAb gene amplification were observed to introduce extensive heterogeneity into the heavy and light chain FR1 regions, N-terminal protein sequence analysis was also conducted to obtain the correct amino acid sequence of this region. In order to confirm the identity of the cloned genes, intact mAbs were resolved by two-dimensional electrophoresis and subject to in-gel tryptic digestion for analysis by both MALDI and nanospray LC-MS/MS. Databases searches using the derived mAb sequences predicted residues comprising CDR loops, identified candidate germline genes, and showed the respective germline genes to accurately predict the N-terminal amino acid residues for each variable region. The above studies report the amino acid sequence of Cyt b-specific mAb variable region genes with high confidence and provide essential information for future efforts at Cyt b structure analysis by resonance energy transfer and X-ray crystallography.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Taylor RM,Maaty WS,Lord CI,Hamilton T,Burritt JB,Bothner B,Jesaitis AJdoi
10.1016/j.molimm.2005.10.022subject
Has Abstractpub_date
2007-01-01 00:00:00pages
625-37issue
4eissn
0161-5890issn
1872-9142pii
S0161-5890(06)00023-Xjournal_volume
44pub_type
杂志文章abstract：:Dense deposit disease (DDD) is strongly associated with the uncontrolled activation of the complement alternative pathway. Factor H (CFH)-deficient (Cfh(-/-)) mice spontaneously develop C3 deposition along the glomerular basement membrane (GBM) with subsequent development of glomerulonephritis with features of DDD, a ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.03.030
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abstract：:Salmonella enterica serovar Typhimurium (S. Typhimurium) changes the structure of its lipopolysaccharide (LPS) in response to the environment. The two main LPS variants found in S. Typhimurium correspond to LPS with a hepta-acylated lipid A (LPS 430) and LPS with modified phosphate groups on its lipid A (LPS 435). We ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.03.003
更新日期：2019-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0161-5890(92)90051-x
更新日期：1992-10-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2010-07-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.08.275
更新日期：2008-12-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.04.025
更新日期：2008-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.04.015
更新日期：2008-01-01 00:00:00
abstract：:Systemic lupus erythematosus (SLE) is a typical autoimmune disease with unclear etiology. Aberrant self-DNA recognition has been proven critical for the initiation of excessive immune responses in lupus. Consistently, we previously reported that a sort of self-DNA, activated lymphocyte-derived DNA (ALD-DNA) could pote...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2015.01.023
更新日期：2015-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：1994-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0161-5890(91)90087-z
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journal_title：Molecular immunology
pub_type： 临床试验,杂志文章
doi：10.1016/j.molimm.2006.09.008
更新日期：2007-03-01 00:00:00
abstract：:Toll-like receptors (TLR) are expressed on the surface or intracellularly by dendritic cells (DC) and recognize specifically different pathogen-associated molecular patterns (PAMPs). Increasing evidence suggests that TLR expressed by DC can cooperate to synergize their functions. Here, we describe the cooperation of T...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.02.021
更新日期：2008-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.05.010
更新日期：2006-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2017.05.028
更新日期：2017-09-01 00:00:00
abstract：:The aim of the study was to explore whether fever-range hyperthermia (FRH) might enhance the anticancer and immunoregulatory activities of protein-bound polysaccharides (PBP), a class of fungus derived immunomodifiers used in the cancer adjuvant therapy. Blood lymphocytes and breast cancer cells (MCF-7) were cultured ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.10.013
更新日期：2016-12-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90071-7
更新日期：1990-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.11.006
更新日期：2017-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2015-12-01 00:00:00
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journal_title：Molecular immunology
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90102-6
更新日期：1990-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：1991-07-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90086-f
更新日期：1990-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：1984-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.11.004
更新日期：2009-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.10.001
更新日期：2011-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90082-m
更新日期：1993-02-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2004.04.010
更新日期：2004-07-01 00:00:00
abstract：:Members of the genus Ebolavirus cause fulminating outbreaks of disease in human and non-human primate populations with a mortality rate up to 90%. To facilitate rapid detection of these pathogens in clinical and environmental samples, robust reagents capable of providing sensitive and specific detection are required. ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.06.437
更新日期：2011-09-01 00:00:00
abstract：:Immunoglobulins (Ig) require correct folding and assembly of both heavy (H) and light (L) chains to form a functional H2L2 dimer that is secreted from plasma cells. This process is dependent upon the endoplasmic reticulum (ER) chaperone BiP, which targets improperly, folded or assembled Ig molecules for degradation. W...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.11.015
更新日期：2005-05-01 00:00:00
