Abstract：
:Leprosy is a bacterial disease caused by M. leprae. Its clinical spectrum reflects the host's immune response to the M. leprae and provide an ideal model to investigate the host pathogen interaction and immunological dysregulation. Tregs are high in leprosy patients and responsible for immune suppression of the host by producing IL-10 and TGF-β cytokines. In leprosy, plasticity of Tregs remain unstudied. This is the first study describing the conversion of Tregs into Th1-like and Th17-like cells using in vitro cytokine therapy in leprosy patients. Peripheral blood mononuclear cells from leprosy patients were isolated and stimulated with M. leprae antigen (MLCwA), rIL-12 and rIL-23 for 48h. Expression of FoxP3 in CD4+CD25+ Tregs, intracellular cytokines IFN-γ, TGF-β, IL-10 and IL-17 in Tregs cells were evaluated by flow cytometry (FACS) after stimulation. rIL-12 treatment increases the levels of pStat4 in Tregs and IFN-γ production. In the presence of rIL-23, pStat3+ and IL-17A+ cells increase. rIL-12 and r-IL-23 treatment downregulated the FoxP3 expression, IL-10 and TGF-β production by Tregs and enhances the expression of co-stimulatory molecules (CD80, CD86). In conclusion rIL-12 converts Tregs into IFN-γ producing cells through STAT-4 signaling while rIL-23 converts Tregs into IL-17 producing cells through STAT-3 signaling in leprosy patients. This study may helpful to provide a new avenue to overcome the immunosuprression in leprosy patients using in vitro cytokine.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Tarique M,Saini C,Naqvi RA,Khanna N,Sharma A,Rao DNdoi
10.1016/j.molimm.2017.01.008subject
Has Abstractpub_date
2017-03-01 00:00:00pages
72-81eissn
0161-5890issn
1872-9142pii
S0161-5890(17)30008-1journal_volume
83pub_type
杂志文章abstract：BACKGROUND:The complement system is well known for its role in innate immunity via the classical, the alternative and the lectin pathways, although recent investigations suggest expanding roles in adipose tissue. Properdin stabilizes C3 convertase following alternative complement activation. Properdin is also present i...
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pub_type： 杂志文章
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
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pub_type： 杂志文章
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更新日期：1985-09-01 00:00:00
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journal_title：Molecular immunology
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更新日期：2011-07-01 00:00:00
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更新日期：2008-01-01 00:00:00
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pub_type： 历史文章,杂志文章,评审
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更新日期：2020-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(99)00122-4
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pub_type： 杂志文章
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更新日期：1985-07-01 00:00:00
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pub_type： 杂志文章
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更新日期：2008-04-01 00:00:00
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pub_type： 杂志文章
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更新日期：2014-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2003-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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journal_title：Molecular immunology
pub_type： 杂志文章
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2020-02-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1016/j.molimm.2015.06.015
更新日期：2015-10-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(97)00146-6
更新日期：1997-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00132-8
更新日期：1996-01-01 00:00:00
