IL-37 induces autophagy in hepatocellular carcinoma cells by inhibiting the PI3K/AKT/mTOR pathway.


:Autophagy is an intracellular "self-eating" process that is closely related to inflammation and cellular immunity. New studies indicate that autophagy is also involved in tumor suppression. The anti-inflammatory cytokine interleukin-37 (IL-37) has been shown to have tumor-suppressive abilities in hepatocellular carcinoma (HCC). Notably, autophagy appears to play a dual role in the development of HCC and may be involved in both tumorigenesis and tumor suppression. However, the potential role of IL-37 in autophagy is currently unknown. In this study, we investigated the effect of IL-37 on autophagy in multiple HCC cell lines. In doing so, we found that IL-37 inhibits proliferation in HCC cells and also induces autophagy and apoptosis in the SMMC-7721 and Huh-7 cell lines. Further experiments revealed that IL-37 treatment reduced the levels of phosphorylated protein kinase B (p-AKT), phosphorylated mammalian target of rapamycin (p-mTOR), phosphorylated p70 ribosomal protein s6 kinase (p-p70S6K) and phosphorylated 4E-binding protein 1 (4E-BP1). Moreover, treatment with an AKT agonist, insulin-like growth factor 1 (IGF-1), reversed these IL-37-mediated effects on autophagy, and treatment with an phosphoinositide-3-kinase (PI3K)/AKT inhibitor, LY294002, mimicked the effects of IL-37. Taken together, these results indicate that IL-37 regulates autophagy in SMMC-7721 and Huh-7 cells via inhibition of the PI3K/AKT/mTOR signaling pathway.


Mol Immunol


Molecular immunology


Li TT,Zhu D,Mou T,Guo Z,Pu JL,Chen QS,Wei XF,Wu ZJ




Has Abstract


2017-07-01 00:00:00












  • The unconventional role of HLA-E: The road less traveled.

    abstract::Histocompatibility Leukocyte Antigens, or HLAs, are one of the most polymorphic molecules in humans. This high degree of polymorphism endows HLA molecules with the ability to present a vast array of peptides, an essential trait for responding to ever-evolving pathogens. Unlike classical HLA molecules (HLA-Ia), some no...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Grant EJ,Nguyen AT,Lobos CA,Szeto C,Chatzileontiadou DSM,Gras S

    更新日期:2020-04-01 00:00:00

  • Transient expression of pentameric IgM on the surface of NZB B cells.

    abstract::In contrast to normal B lymphocytes, splenic B cells from autoimmune NZB mice express on their surface greater amounts of IgM than IgD. We have found that the predominant mu chain species present on these cells is derived from pentameric secretory IgM which can be radioiodinated during transient association with the c...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Yuan D,Goldings EA

    更新日期:1988-04-01 00:00:00

  • Engineered protein containing crotoxin epitopes induces neutralizing antibodies in immunized rabbits.

    abstract::Crotoxin (Ctx) is the main lethal component of Crotalus durissus terrificus venom. It is a neurotoxin, composed of two subunits associated by noncovalent interactions, the non-toxic acid subunit (CA), named Crotapotin, and the basic subunit (CB), with phospholipase A2 (PLA2) activity. Employing the SPOT synthesis tech...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Molina Molina DA,Guerra-Duarte C,Costal-Oliveira F,Almeida Rocha E,Rego Rodrigues C,Machado-de-Ávila RA,Soccol VT,Chávez-Olórtegui C

    更新日期:2020-03-01 00:00:00

  • Dmu expression causes enrichment of MZ B cells, but is non permissive for B cell maturation in Rag2-/- mice even if combined with Bcl-2.

    abstract::Rearrangements in reading frame 2 promote the expression of a truncated heavy chain, the Dmu protein. Dmu can assemble into a pre-B cell receptor like complex that appears to induce a subset of signals elicited by full length mu, but cannot promote the pro-B to pre-B cell transition of Rag-/- B cells. In order to dete...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Wikström I,Bergqvist I,Holmberg D,Forssell J

    更新日期:2006-03-01 00:00:00

  • A region of the N-terminal domain of meningococcal factor H-binding protein that elicits bactericidal antibody across antigenic variant groups.

    abstract::Meningococcal factor H-binding protein (fHbp) is a promising vaccine antigen. Previous studies described three fHbp antigenic variant groups and identified amino acid residues between 100 and 255 as important targets of variant-specific bactericidal antibodies. We investigated residues affecting expression of an epito...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Beernink PT,LoPasso C,Angiolillo A,Felici F,Granoff D

    更新日期:2009-05-01 00:00:00

  • The heterogeneity of bovine IgG2--IV. Structural differences between IgG2a molecules of the A1 and A2 allotypes.

    abstract::The major product of digestion of bovine IgG2a(A1) with immobilized pepsin is F(ab')2 while similar treatment of IgG2a(A2) yields Fab, Fc and other products. It is postulated that structural differences in the hinge region, including the absence of the most N-terminal disulfide bridge in IgG2a(A2) and a displacement o...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Heyermann H,Butler JE

    更新日期:1987-12-01 00:00:00

  • Fever-range hyperthermia inhibits cells immune response to protein-bound polysaccharides derived from Coriolus versicolor extract.

    abstract::The aim of the study was to explore whether fever-range hyperthermia (FRH) might enhance the anticancer and immunoregulatory activities of protein-bound polysaccharides (PBP), a class of fungus derived immunomodifiers used in the cancer adjuvant therapy. Blood lymphocytes and breast cancer cells (MCF-7) were cultured ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Pawlikowska M,Jędrzejewski T,Piotrowski J,Kozak W

    更新日期:2016-12-01 00:00:00

  • Prolonged survival effects induced by immature dendritic cells and regulatory T cells in a rat liver transplantation model.

    abstract:BACKGROUND:Dendritic cells (DCs) and regulatory T (Treg) cells are crucial for inducing immune tolerance. However, the suppressive function of infused Treg cells and immature DCs (imDCs) following solid organ transplantation remains unclear. METHODS:ImDCs derived from DA-donor rats and Treg cells isolated from spleens...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: He W,Chen L,Zheng L,Luo L,Gao L

    更新日期:2016-11-01 00:00:00

  • Leucine-rich repeat-containing G-protein-coupled receptor 2 (LGR2) can regulate PO activity and AMP genes expression in Macrobrachium nipponense.

    abstract::Leucine-rich repeat-containing G-protein-coupled receptors (LGRs) form a subfamily of the large superfamily of G-protein-coupled receptors. LGRs can be divided into three groups. LGR2 from Drosophila melanogaster is involved in cuticle tanning (melanization and sclerotization). In this study, one LGR2 (MnLGR2) was ide...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Cao X,Zhang C,Zhang R,Wang K,Dai X,Huang X,Ren Q

    更新日期:2020-10-01 00:00:00

  • A simple procedure for the isolation of human secretory IgA of IgA1 and IgA2 subclass by a jackfruit lectin, jacalin, affinity chromatography.

    abstract::Jackfruit lectin, jacalin, prepared from two batches of jackfruit seeds showed a different specificity in precipitating reaction in Agarose gel with various purified immunoglobulins and secretory components. Jacalin-P, extracted from jackfruit seeds from the Philippines, reacts only with serum IgA and secretory IgA of...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Kondoh H,Kobayashi K,Hagiwara K

    更新日期:1987-11-01 00:00:00

  • Functional mapping and single chain construction of the anti-cytokeratin 8 monoclonal antibody TS1.

    abstract::The anti-cytokeratin (CK) 8 monoclonal antibody (mab) TS1 has been shown to efficiently bind to CK8 expressed in carcinomas in vivo. The anti-idiotypic antibody of TS1, alphaTS1, can be used to regulate the tumor:non-tumor ratio of TS1 by clearing non-tumor binding TS1 from the circulation. If the interaction of TS1 t...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Holm P,Jafari R,Sundström BE

    更新日期:2007-02-01 00:00:00

  • Hydrophilic-amphiphilic transition of the terminal SC5b-8 complement complex through tryptic modification: biochemical and ultrastructural studies.

    abstract::the SC5b-8 complex of human complement is a hydrophilic molecule of mol. wt 800,000-850,000 that is seen in the electron microscope as an elongated, straight or curved structure of 50-55 nm total length and 8-9 nm width. Tryptic attack on the fluid-phase complex exposes lipid-binding surfaces on the molecule. The tryp...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Bhakdi S,Tranum-Jensen J

    更新日期:1982-09-01 00:00:00

  • Non-traditional roles of complement in type 2 diabetes: Metabolism, insulin secretion and homeostasis.

    abstract::Type 2 Diabetes (T2D) is a disease of increasing importance and represents a growing burden on global healthcare and human health. In T2D, loss of effectiveness of insulin signaling in peripheral tissues cannot be compensated for by adequate insulin secretion, leading to hyperglycemia and resultant complications. In r...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: King BC,Blom AM

    更新日期:2017-04-01 00:00:00

  • High molecular weight components containing N-linked oligosaccharides of Ascaris suum extract inhibit the dendritic cells activation through DC-SIGN and MR.

    abstract::Helminths, as well as their secretory/excretory products, induce a tolerogenic immune microenvironment. High molecular weight components (PI) from Ascaris suum extract down-modulate the immune response against ovalbumin (OVA). The PI exerts direct effect on dendritic cells (DCs) independent of TLR 2, 4 and MyD88 molec...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Favoretto BC,Casabuono AAC,Portes-Junior JA,Jacysyn JF,Couto AS,Faquim-Mauro EL

    更新日期:2017-07-01 00:00:00

  • MiR-143-3p controls TGF-β1-induced cell proliferation and extracellular matrix production in airway smooth muscle via negative regulation of the nuclear factor of activated T cells 1.

    abstract::MicroRNAs (miRNAs) are small noncoding RNAs that function in diverse biological processes. However, little is known about the precise role of microRNAs in the functioning of airway smooth muscle cells (ASMCs). Here, we investigated the potential role and mechanisms of the miR-143 -3p on proliferation and the extracell...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Cheng W,Yan K,Xie LY,Chen F,Yu HC,Huang YX,Dang CX

    更新日期:2016-10-01 00:00:00

  • Identification of a common HLA-A*0201-restricted epitope among SSX family members by mimicking altered peptide ligands strategy.

    abstract::The synovial sarcoma X breakpoint (SSX) gene family contains nine members. The SSX proteins are CT (cancer/testis) antigens and can be expressed in many tumor types. T cell immune response against SSX protein can be detected in tumor patients and mice expressing any SSX. Screening predominant protective epitopes might...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: He Y,Mao L,Lin Z,Deng Y,Tang Y,Jiang M,Li W,Jia Z,Wang J,Ni B,Wu Y

    更新日期:2008-05-01 00:00:00

  • Comprehensive analysis of lncRNA-mRNA regulatory network in BmNPV infected cells treated with Hsp90 inhibitor.

    abstract::Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the main pathogens that seriously affect the sustainable development of sericulture industry. Inhibition of Hsp90 by Hsp90 inhibitor, geldanamycin (GA) significantly suppresses BmNPV proliferation in Bombyx mori, while the functional mechanism is not clear. LncRNA has...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Yin H,Shang Q,Zhang S,Shen M,Huang H,Zhao W,Xijie G,Wu P

    更新日期:2020-11-01 00:00:00

  • Using agonists for iNKT cells in cancer therapy.

    abstract::The capacity of α-galactosylceramide (α-GalCer) to act as an anti-cancer agent in mice through the specific stimulation of type I NKT (iNKT) cells has prompted extensive investigation to translate this finding to the clinic. However, low frequencies of iNKT cells in cancer patients and their hypo-responsiveness to rep...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Painter GF,Burn OK,Hermans IF

    更新日期:2021-02-01 00:00:00

  • The role of glycosylation in secretion and membrane expression of immunoglobulins M and A.

    abstract::The role of glycosylation in membrane expression and secretion of IgM and IgA was investigated in murine lymphoma and hybridoma cell lines, derived from I.29 tumor, which synthesize IgM or IgA with identical variable regions. Tunicamycin, a selective inhibitor of N-linked glycosylation, prevented the membrane expressi...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Sitia R,Rubartelli A,Hämmerling U

    更新日期:1984-08-01 00:00:00

  • p38 mitogen-activated protein kinases (MAPKs) are involved in intestinal immune response to bacterial muramyl dipeptide challenge in Ctenopharyngodon idella.

    abstract::The p38 mitogen-activated protein kinases (MAPKs) are essential cytoplasmic signal molecules of innate immune pathways that play a vital role in host immune defense responses to pathogenic challenges. In this study, two fish p38 genes (Cip38α and Cip38β) were characterized for the first time from the grass carp Ctenop...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Sun Y,Xu W,Li D,Zhou H,Qu F,Cao S,Tang J,Zhou Y,He Z,Li H,Zhou Z,Liu Z

    更新日期:2020-02-01 00:00:00

  • Vγ1+γδT, early cardiac infiltrated innate population dominantly producing IL-4, protect mice against CVB3 myocarditis by modulating IFNγ+ T response.

    abstract::Viral myocarditis (VMC) is an inflammation of the myocardium closely associated with Coxsackievirus B3 (CVB3) infection. Vγ1+γδT cells, one of early cardiac infiltrated innate population, were reported to protect CVB3 myocarditis while the precise mechanism not fully addressed. To explore cytokine profiles and kinetic...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Wan F,Yan K,Xu D,Qian Q,Liu H,Li M,Xu W

    更新日期:2017-01-01 00:00:00

  • NLRX1 attenuates apoptosis and inflammatory responses in myocardial ischemia by inhibiting MAVS-dependent NLRP3 inflammasome activation.

    abstract::Increasing evidence suggests that inflammation and apoptosis are involved in the development of acute myocardial ischemia (AMI). Nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) have recently been identified as key mediators of inflammatory responses. The aim of this study was to explore the speci...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Li H,Zhang S,Li F,Qin L

    更新日期:2016-08-01 00:00:00

  • Identification and characterization of a novel nanobody against human placental growth factor to modulate angiogenesis.

    abstract::Placental growth factor (PlGF), a member of vascular endothelial growth factors (VEGF) family, is considered as an important antigen associated with pathological conditions such as cancer cell growth, and metastasis. PlGF-targeting via nanobody (Nb) therefore could be beneficial to modulate these pathologies. In this ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Arezumand R,Mahdian R,Zeinali S,Hassanzadeh-Ghassabeh G,Mansouri K,Khanahmad H,Namvar-Asl N,Rahimi H,Behdani M,Cohan RA,Eavazalipour M,Ramazani A,Muyldermans S

    更新日期:2016-10-01 00:00:00

  • Ecto-F1-ATPase and MHC-class I close association on cell membranes.

    abstract::Subunits of the mitochondrial ATP synthase complex are expressed on the surface of tumors, bind the TCR of human Vgamma9/Vdelta2 lymphocytes and promote their cytotoxicity. Present experiments show that detection of the complex (called ecto-F1-ATPase) at the cell surface by immunofluorescence correlates with low MHC-c...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Vantourout P,Martinez LO,Fabre A,Collet X,Champagne E

    更新日期:2008-01-01 00:00:00

  • A new effector of lipid metabolism: complement factor properdin.

    abstract:BACKGROUND:The complement system is well known for its role in innate immunity via the classical, the alternative and the lectin pathways, although recent investigations suggest expanding roles in adipose tissue. Properdin stabilizes C3 convertase following alternative complement activation. Properdin is also present i...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Gauvreau D,Roy C,Tom FQ,Lu H,Miegueu P,Richard D,Song WC,Stover C,Cianflone K

    更新日期:2012-05-01 00:00:00

  • Regulation and functions of NLRP3 inflammasome during influenza virus infection.

    abstract::The NLRP3 inflammasome constitutes a major antiviral host defense mechanism during influenza virus infection. Inflammasome assembly in virus-infected cells facilitates autocatalytic processing of pro-caspase-1 and subsequent cleavage and secretion of proinflammatory cytokines IL-1β and IL-18. The NLRP3 inflammasome is...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Kuriakose T,Kanneganti TD

    更新日期:2017-06-01 00:00:00

  • Influence of terminal residue on adjacent disaccharide immunogenicity.

    abstract::Aberrant O-glycosylation of cell surface mucin antigens is characteristic of epithelial cancer cells. For example, Thomsen-Friedenreich disaccharide (TFD) is a chemically well-defined carbohydrate antigen with a documented link to malignancy. There have been many attempts to improve immune response to carbohydrate ant...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Irazoqui FJ,Lopez PH,Mandel U,Nores GA

    更新日期:2002-03-01 00:00:00

  • Generation of a comprehensive panel of crustacean allergens from the North Sea Shrimp Crangon crangon.

    abstract:BACKGROUND:Published data on crustacean allergens are incomplete. The identification of tropomyosin (TM), arginine kinase (AK), sarcoplasmic Ca-binding protein (SCP) and myosin light chain (MLC) as shrimp allergens are all important contributions but additional allergens are required for the development of a complete s...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Bauermeister K,Wangorsch A,Garoffo LP,Reuter A,Conti A,Taylor SL,Lidholm J,Dewitt AM,Enrique E,Vieths S,Holzhauser T,Ballmer-Weber B,Reese G

    更新日期:2011-09-01 00:00:00

  • Chemokine-mediated inflammation: Identification of a possible regulatory role for CCR2.

    abstract::The chemokine receptor CCR2 binds four pro-inflammatory monocyte chemoattractant proteins, designated MCP1/CCL2, MCP2/CCL8, MCP3/CCL7 and MCP4/CCL13. This study demonstrates the important biology of this receptor during the response to the chemokine milieu. Competitive chemotaxis and calcium flux assays were performed...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: O'Boyle G,Brain JG,Kirby JA,Ali S

    更新日期:2007-03-01 00:00:00

  • Anti-allergic properties of a new all-D synthetic immunoglobulin-binding peptide.

    abstract::Using a combinatorial chemistry approach, we identified a tetrameric tripeptide, denoted Protein A Mimetic (PAM) or TG19318, able to bind to immunoglobulins of different classes and species. The inverso variant, with the tripeptide in the all-D configuration (D-PAM or TG19320), is described as retaining binding proper...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Rossi M,Ruvo M,Marasco D,Colombo M,Cassani G,Verdoliva A

    更新日期:2008-01-01 00:00:00