Abstract:
:Autophagy is an intracellular "self-eating" process that is closely related to inflammation and cellular immunity. New studies indicate that autophagy is also involved in tumor suppression. The anti-inflammatory cytokine interleukin-37 (IL-37) has been shown to have tumor-suppressive abilities in hepatocellular carcinoma (HCC). Notably, autophagy appears to play a dual role in the development of HCC and may be involved in both tumorigenesis and tumor suppression. However, the potential role of IL-37 in autophagy is currently unknown. In this study, we investigated the effect of IL-37 on autophagy in multiple HCC cell lines. In doing so, we found that IL-37 inhibits proliferation in HCC cells and also induces autophagy and apoptosis in the SMMC-7721 and Huh-7 cell lines. Further experiments revealed that IL-37 treatment reduced the levels of phosphorylated protein kinase B (p-AKT), phosphorylated mammalian target of rapamycin (p-mTOR), phosphorylated p70 ribosomal protein s6 kinase (p-p70S6K) and phosphorylated 4E-binding protein 1 (4E-BP1). Moreover, treatment with an AKT agonist, insulin-like growth factor 1 (IGF-1), reversed these IL-37-mediated effects on autophagy, and treatment with an phosphoinositide-3-kinase (PI3K)/AKT inhibitor, LY294002, mimicked the effects of IL-37. Taken together, these results indicate that IL-37 regulates autophagy in SMMC-7721 and Huh-7 cells via inhibition of the PI3K/AKT/mTOR signaling pathway.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Li TT,Zhu D,Mou T,Guo Z,Pu JL,Chen QS,Wei XF,Wu ZJdoi
10.1016/j.molimm.2017.04.010subject
Has Abstractpub_date
2017-07-01 00:00:00pages
132-140eissn
0161-5890issn
1872-9142pii
S0161-5890(17)30109-8journal_volume
87pub_type
杂志文章abstract::The mechanisms of each subset of immune cells contributing to the pathogenesis of viral hepatitis remain incompletely understood. In this study, we examined the role of liver CD4(-) CD8(-) (double negative, DN) T cells during murine hepatitis virus strain 3 (MHV-3)-induced hepatitis in C3H/HeJ mice. We demonstrate tha...
journal_title:Molecular immunology
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doi:10.1016/j.molimm.2012.05.017
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abstract::The antigen-limiting nature of microtiter ELISAs predicts that antibodies of minor classes may be underestimated when the same specimen contains large amounts of IgG antibodies specific for the same antigen. Such competitive inhibition can be diagnosed from ELISA titration plots. A method is described to eliminate the...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(90)90044-z
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journal_title:Molecular immunology
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doi:10.1016/0161-5890(92)90045-y
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pub_type: 杂志文章
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journal_title:Molecular immunology
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(89)90078-3
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journal_title:Molecular immunology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1993-12-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.02.027
更新日期:2010-06-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.11.011
更新日期:2011-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular immunology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular immunology
pub_type: 杂志文章
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2009-06-01 00:00:00