Abstract：
:Phosphorothioate oligonucleotides (S-oligos) are nucleic acid derivatives that are commonly used as antisense agents. These compounds, similar to bacterial DNA and CpG oligonucleotides, display a variety of immunological activities in vitro and in vivo. To assess further these activities, the antigenicity of a series of S-oligos was assessed in the solid phase using anti-DNA antibodies from sera of patients with systemic lupus erythematosus. By ELISA, S-oligos bound well to anti-DNA antibodies under the same conditions as calf thymus DNA antigen. The specificity for anti-DNA was established by competition assays showing cross-inhibition of binding by DNA and S-oligos. Reactivity with anti-DNA was observed with S-oligos varying in sequence, suggesting interaction with a conserved determinant not strictly dependent on the bases. Furthermore. in comparison with a phosphodiester oligomer of the same sequence, a phosphorothioate showed dramatically increased activity. These findings indicate that structural features associated with the S-oligo backbone promote specific binding to anti-DNA antibodies and influence the size requirement for antigenicity in the solid phase. These observations thus extend the immunological properties of S-oligos and suggest uses of these compounds in the diagnosis and treatment of autoimmune disease.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Pisetsky DS,Reich CF 3rddoi
10.1016/s0161-5890(98)00108-4subject
Has Abstractpub_date
1998-12-01 00:00:00pages
1161-70issue
18eissn
0161-5890issn
1872-9142pii
S0161589098001084journal_volume
35pub_type
杂志文章abstract：:The ubiquitin conjugating (ubc) E2 enzyme ubc-9 conjugates the ubiquitin-like peptide sentrin/SUMO-1/PIC1 to target proteins which include the Fas antigen. We show that the mouse genome contains four copies of the ubc-9 gene. These include a structural ubc-9 gene consisting of seven exons which encode a protein identi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(98)00094-7
更新日期：1998-11-01 00:00:00
abstract：:Prohibitin (PHB1) is a multifunction protein that is released in lipid droplets from adipocytes and possibly other cells and is detectable in the circulation. We used crosslinking, immunoprecipitation and proteomic analysis to investigate binding partners for circulating PHB1. Crosslinking of PHB1 to serum resulted in...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.09.025
更新日期：2007-03-01 00:00:00
abstract：:Protein kinases have been implicated in a number of regulatory mechanisms including signal transduction in many cells. To address the possibility that the large granular lymphocyte (LGL) also uses one or more unique protein kinases for LGL functions, an efficient method was developed to obtain partial cDNA clones for ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90153-b
更新日期：1991-04-01 00:00:00
abstract：:Forty-eight group A streptococcal strains of different M types were screened for binding of human radiolabeled IgG. Three of the strains bound more than 80% of the added radioactivity and one of them, an M protein type 1 strain designated AP1, was selected for further analysis. Attempts were made to solubilize the IgG...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90071-7
更新日期：1990-06-01 00:00:00
abstract：:Serum levels of IgA1 and IgA2 were measured by solid phase radioimmunoassay in samples from 110 children between 3 months and 10 years of age. Both IgA1 and IgA2 were detectable in all samples, and both IgA1 and IgA2 increased with increasing age. The percent of total serum IgA that was IgA2 did not change with age an...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90038-x
更新日期：1983-09-01 00:00:00
abstract：:C1q, the recognition molecule of the classical pathway of complement, binds to endothelial cells, leading to cell activation. Mannose-binding lectin (MBL), a recognition molecule of the lectin pathway, is structurally and functionally related to C1q. Therefore, we investigated the interaction of MBL with human umbilic...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.06.015
更新日期：2007-02-01 00:00:00
abstract：:Candida albicans the most frequently isolated clinical fungal pathogen can cause local as well as systemic and life-threatening infections particularly in immune-compromised individuals. A better and more detailed understanding how C. albicans evades human immune attack is therefore needed for identifying fungal immun...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.07.010
更新日期：2018-01-01 00:00:00
abstract：:The immune system protects from infections primarily by detecting and eliminating invading pathogens. This is predominantly mediated by innate immune cells like neutrophils, monocytes and dendritic cells (DCs) expressing specific receptors recognizing pathogen-associated molecular patterns. DC activation by pathogens ...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2020.02.015
更新日期：2020-05-01 00:00:00
abstract：:The site of the murine IgG1 molecule that regulates catabolism has recently been shown to encompass amino acids that are located at the CH2-CH3 domain interface. The CH2 and CH3 domains are connected to each other by a relatively flexible "mini-hinge" region, and flexibility in this region could clearly affect the ori...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00019-b
更新日期：1995-05-01 00:00:00
abstract：:Previous work on the cis-acting elements that control heavy chain variable region (VH) gene somatic hypermutation has indicated the presence of an as yet unidentified element(s) 3' of the intron enhancer that is necessary for high rate mutation. Examination of cis-acting elements involved in kappa light chain V gene h...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(97)00065-5
更新日期：1997-04-01 00:00:00
abstract：:We have investigated the chemical structure of the antigenic determinant of Candida krusei cell-wall mannan. Acetolysis of the mannan, obtained by extraction with alkali and purified as copper complex, gave five oligosaccharides (from mono- to pentasaccharide) and a minor amount of an octasaccharide. Partial acetolysi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90202-4
更新日期：1982-03-01 00:00:00
abstract：:To understand the mechanism of action of the chaperone protein tapasin, which mediates loading of high-affinity peptides onto major histocompatibility complex (MHC) class I molecules in the antiviral immune response, we have performed numerical simulations of the class I-peptide binding process with four different mec...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.02.032
更新日期：2009-06-01 00:00:00
abstract：:V(D)J recombination is a mechanism peculiar to the somatic rearrangement of antigen receptor genes. It requires both expression of the RAG-1 and RAG-2 recombinases and accessibility of the substrate to its recombinase and post-cleavage/DNA repair stage. TCR revision is a genetic correction mechanism that changes T cel...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.06.153
更新日期：2008-01-01 00:00:00
abstract：:The anti-cytokeratin (CK) 8 monoclonal antibody (mab) TS1 has been shown to efficiently bind to CK8 expressed in carcinomas in vivo. The anti-idiotypic antibody of TS1, alphaTS1, can be used to regulate the tumor:non-tumor ratio of TS1 by clearing non-tumor binding TS1 from the circulation. If the interaction of TS1 t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.08.001
更新日期：2007-02-01 00:00:00
abstract：:ANCA associated vasculitis is a serious, very often recurrent disease that despite the current standard treatment with high-dose glucocorticoids and either cyclophosphamide or rituximab, patients have a nine-fold increased mortality risk in the first year compared with healthy controls, attributed to infections, vascu...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2019.06.018
更新日期：2019-08-01 00:00:00
abstract：:Maspin (mammary serine protease inhibitor) is a non-inhibitory member of the serine protease inhibitor superfamily and a tumor suppressor in several cancers due to its ability to inhibit cell invasion, angiogenesis, and promote apoptosis. However, its immunomodulatory function remains largely unexplored. Thus, we expl...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.12.021
更新日期：2017-02-01 00:00:00
abstract：:Glycosylation is an important protein post-translational modification. In this process, the intermediate product, Tn antigen, arises from somatic mutations in core1β3-galactosyltransferase-specific molecular chaperone (Cosmc), which is required for the formation of active core1β3-galactosyltransferase (T-synthase). As...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.06.250
更新日期：2017-10-01 00:00:00
abstract：:The purpose of this study was to evaluate the effects of GATA-6 on airway inflammation and remodeling and the underlying mechanisms in a murine model of chronic asthma. Female BALB/c mice were randomly divided into four groups: phosphate-buffered saline control (PBS), ovalbumin (OVA)-induced asthma group (OVA), OVA+ s...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.05.017
更新日期：2016-07-01 00:00:00
abstract：:Previous data suggested that constitutive expression of the transcription factor Bright (B cell regulator of immunoglobulin heavy chain transcription), normally tightly regulated during B cell differentiation, was associated with autoantibody production. Here we show that constitutive Bright expression results in skew...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.09.008
更新日期：2011-10-01 00:00:00
abstract：:The analysis of the antigenic structure of human interferon (IFN)-alpha 1 with a panel of monoclonal antibodies revealed four immunodominant regions. Three of them, recognized by 12 of 14 antibodies were mapped into the aminoterminal portion of IFN-alpha 1 around residues 31-38, 43-53 and 63-85. The region 31-85 prove...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90124-g
更新日期：1992-07-01 00:00:00
abstract：:Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb), leading to high mortality worldwide. It is well-established that cellular immunity plays a critical role to control Mtb infection. Dendritic Cells (DCs) are potent antigen presenting cells, which play an important role to prime cell...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.04.014
更新日期：2016-07-01 00:00:00
abstract：:This report demonstrates that a marker of human embryonic endoderm and embryonal carcinoma cells recognized by a hybridoma antibody FC 10.2, involves Type 1 blood group chains with the sequence Gal beta 1 leads to 3G1cNAc beta 1 leads to 3Gal beta 1 leads to 4G1c. This conclusion has been reached from antigenic analys...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90005-6
更新日期：1983-06-01 00:00:00
abstract：:Twenty-seven murine monoclonal anti-hen egg-white lysozyme (HEL) antibodies were tested with a panel of nine antigens; eight avian lysozymes and human lysozyme. The antibodies were arranged into 10 groups based on their antigen specificity and cross-reactivity. Antigenic determinants recognized by each group of antibo...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90081-2
更新日期：1987-02-01 00:00:00
abstract：:Inhibition of the catalytic activity of horseradish peroxidase by rabbit antisera was measured using alternate enzyme substrates. The same general inhibition curve patterns were obtained regardless of the electron donor or hydroperoxide used. In all cases typical biphasic inhibition patterns were found and complete in...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90169-4
更新日期：1983-12-01 00:00:00
abstract：:Evidence has been accumulating for a role of inflammation in the development of Alzheimer's disease (AD), a progressive neurodegenerative disorder causing a common form of dementia in the elderly. C1q, part of the initiation component of the classical complement pathway (CCP), is associated with beta-sheet, fibrillar ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.02.022
更新日期：2008-06-01 00:00:00
abstract：:Interferon (IFN)-γ is highly upregulated during heart inflammation and enhances the production of pro-inflammatory cytokines. Absent in Melanoma 2 (AIM2) is an IFN-inducible protein implicated as a component of the inflammasome. Here we seek to determine the role of AIM2 during inflammation in cardiac cells. We found ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.04.009
更新日期：2016-06-01 00:00:00
abstract：:Malignant transformation of hepatocytes is frequently associated with upregulation of HLA-A expression. Currently there is no information available regarding the mechanisms underlying this phenotypic change. We investigated HLA-A expression in 165 paraffin embedded tissues and 21 fresh tissues from liver cancer patien...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.03.001
更新日期：2009-06-01 00:00:00
abstract：:20C, a novel bibenzyl compound, is isolated from Gastrodia elata. In our previous study, 20C showed protective effects on tunicamycin-induced endoplasmic reticulum stress, rotenone-induced apoptosis and rotenone-induced oxidative damage. However, the anti-neuroinflammatory effect of 20C is still with limited acquainta...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2018.04.014
更新日期：2018-07-01 00:00:00
abstract：:Some IgM cattle antibodies are amongst the largest known to exist in jawed vertebrates where CDR3H size may extend up to 61 amino acids. To understand the origin of such an exceptionally long CDR3H, bovine D(H) gene locus was completely characterized from Holstein cattle that revealed the presence of a total of eight ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.02.014
更新日期：2010-07-01 00:00:00
abstract：:BST-2 (also known as tetherin, CD317, or HM1.24) was first described as a potent interferon-inducible host antiviral factor nearly five years ago. Since that time, numerous reports have been published regarding the antiviral activity and immunological properties of this protein. BST-2 blocks viral replication by inhib...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2012.11.008
更新日期：2013-06-01 00:00:00
