Abstract：
:Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb), leading to high mortality worldwide. It is well-established that cellular immunity plays a critical role to control Mtb infection. Dendritic Cells (DCs) are potent antigen presenting cells, which play an important role to prime cell-mediated immune responses. In vivo targeting of DCs has been shown to induce both strong cellular immunity and protection against tumor challenges. The aim of the present study was not only to assess the immunizing potential of a novel DC-targeted recombinant lentivirus expressing fusion antigen Ag85A-ESAT6 of Mtb, but also to compare it with a recombinant lentivirus with broad cellular tropism expressing the same antigen in mice. The findings demonstrated that our novel recombinant DC-targeted lentivector was able to successfully transduce and express the fusion antigen Ag85A-E6 in vitro and in vivo. Moreover, a single footpad injection of targeted lentivectors could elicit strong T-helper 1 (Th1) immunity against the above mentioned antigen, as indicated by the specific high-level production of IFN-γ and IL-2 using spleen lymphocytes and lymphoproliferative responses. Despite of these promising results, more attempts are required to elucidate the protective and therapeutic efficacy of this approach in future.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Shakouri M,Moazzeni SM,Ghanei M,Arashkia A,Etemadzadeh MH,Azadmanesh Kdoi
10.1016/j.molimm.2016.04.014subject
Has Abstractpub_date
2016-07-01 00:00:00pages
101-11eissn
0161-5890issn
1872-9142pii
S0161-5890(16)30073-6journal_volume
75pub_type
杂志文章abstract：:Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the main pathogens that seriously affect the sustainable development of sericulture industry. Inhibition of Hsp90 by Hsp90 inhibitor, geldanamycin (GA) significantly suppresses BmNPV proliferation in Bombyx mori, while the functional mechanism is not clear. LncRNA has...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.09.014
更新日期：2020-11-01 00:00:00
abstract：:In two recent articles published in Molecular Immunology, Steere and colleagues continue their search for an autoimmune mechanism of arthritis in patients who have failed short-course antibiotic therapy for Borrelia burgdorferi, the spirochetal agent of Lyme disease. As in previous attempts, the authors fail to elucid...
journal_title：Molecular immunology
pub_type： 评论,信件
doi：10.1016/j.molimm.2008.02.015
更新日期：2008-06-01 00:00:00
abstract：:Histocompatibility Leukocyte Antigens, or HLAs, are one of the most polymorphic molecules in humans. This high degree of polymorphism endows HLA molecules with the ability to present a vast array of peptides, an essential trait for responding to ever-evolving pathogens. Unlike classical HLA molecules (HLA-Ia), some no...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2020.02.011
更新日期：2020-04-01 00:00:00
abstract：:Mannose-binding lectin (MBL) is a recognition molecule of the complement (C) system and binds to carbohydrate ligands present on a wide range of pathogenic bacteria, viruses, fungi, and parasites. MBL has been detected in the cervico-vaginal cavity where it can provide a first-line defence against infectious agents co...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.07.016
更新日期：2010-11-01 00:00:00
abstract：:Many infections are associated with diabetes, as the ability of the body to fight pathogens is impaired. Recently, altered levels of defensins and polymorphism in Toll-like receptors (TLRs), two crucial components of the innate immune system, have been associated with diabetes. To study the functionality of the innate...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.04.009
更新日期：2007-02-01 00:00:00
abstract：:Interplay between complement factors, regulatory proteins, anaphylatoxins and cytokines could be involved in tendon healing and scar formation. The expression and regulation of complement factors by cytokines or anaphylatoxins are completely unclear in tendon. Hence, the gene expression of the anaphylatoxin receptors ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.09.001
更新日期：2013-04-01 00:00:00
abstract：:We have examined the regulation of the AP-1 DNA transcription complex during T cell activation in response to interleukin 1 (IL-1) and phorbol ester (TPA) treatment of the IL-1 responsive murine thymoma T cell line, EL4 6.1 C 10. IL-1 synergistically enhances the stimulatory effect of TPA on AP-1-mediated gene express...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00055-j
更新日期：1995-08-01 00:00:00
abstract：:Osteopontin (OPN) is a cytokine with multiple functions, including the regulation of innate immune response. However, the detailed function and mechanism of OPN in host defense against invaded microorganisms remain unclear. In this report, we revealed that OPN could affect the clearance of Propionibacterium acnes in k...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.06.435
更新日期：2011-09-01 00:00:00
abstract：:Bcl-2 translocations in follicular lymphomas (FL) are often associated with downstream immunoglobulin class switch recombination (CSR) on the translocated allele. We studied cell lines with different translocations into the IgH locus to gauge any common features associated with downstream CSR events. CSR associated wi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2003.09.001
更新日期：2003-12-01 00:00:00
abstract：:Rabbits hyperimmunized with fluorescyl-conjugated KLH exhibited bound ligand associated with a high affinity circulating IgG anti-fluorescein population. After cessation of immunogen administration the liganded complexes were eventually spontaneously cleared from the circulation. Individual rabbits synthesized autolog...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90105-7
更新日期：1992-02-01 00:00:00
abstract：:Serum levels of IgA1 and IgA2 were measured by solid phase radioimmunoassay in samples from 110 children between 3 months and 10 years of age. Both IgA1 and IgA2 were detectable in all samples, and both IgA1 and IgA2 increased with increasing age. The percent of total serum IgA that was IgA2 did not change with age an...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90038-x
更新日期：1983-09-01 00:00:00
abstract：:Inhibition of C5a by antibodies has been demonstrated to dramatically improve survival in various sepsis models in mice and rats. The structural basis of C5a mediated bioactivity and C5a antibody mediated neutralization are of interesting to be investigated. In the previous study, we obtained a novel functional mouse ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.03.013
更新日期：2011-07-01 00:00:00
abstract：:A number of fragments derived from acid-treated rabbit IgG by digestion with plasmin have been separated by high-resolution gel filtration. Fragments isolated included a dimer and monomer Facb, named F(acb)2 and Facb, respectively and a heterodimer composed of Facb and Fab subunits, named F(acb)(ab). A C gamma 2 fragm...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90147-6
更新日期：1985-07-01 00:00:00
abstract：BACKGROUND:Bladder cancer (BC) can be successfully treated by manipulating immune responses with intravesical Bacillus Calmette-Guerin instillation or targeting the PD-1/PD-L signaling pathway. In the present study we investigated the prognostic significance of the immune checkpoint inhibitor PD-1 and its ligands PD-L1...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.05.010
更新日期：2020-08-01 00:00:00
abstract：:Purified mRNA's encoding the HLA-A and -B antigen heavy chains or beta 2-microglobulin were prepared from human B lymphoid cells by positive hybridization selection procedures. The role of chain association in the biosynthesis and intracellular transport of HLA-A and -B antigens was investigated by injecting these mRN...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90115-x
更新日期：1986-05-01 00:00:00
abstract：:Mammalian B-cell specific somatic hypermutation contributes to affinity maturation of the antibody response. This mutator activity is highly focused on rearranged immunoglobulin variable regions, but the underlying mechanism remains to be elucidated. In an effort to gain insights into the mechanism of somatic hypermut...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90081-7
更新日期：1994-02-01 00:00:00
abstract：:Monoclonal antibodies (MAbs) raised against trout immunoglobulin (Ig) recognize two independent Ig populations differing in the molecular weight of L chains. The trout Ig population recognized by MAb 2H9 represented congruent to 20% of the total Ig and contained L chains congruent to 26 kDa, whereas the trout Ig popul...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90014-b
更新日期：1991-11-01 00:00:00
abstract：:To date, although a promising anti-inflammatory activity of oligomeric proanthocyanidins (OPCs) has been observed in asthma, the mechanism responsible for these immunomodulatory properties remains obscure. Dendritic cells (DCs) that reside in the airway have been widely perceived as an important contributor to asthma....
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.09.012
更新日期：2017-11-01 00:00:00
abstract：:Grass pollens are amongst the most important aeroallergen sources world-wide triggering allergic rhinoconjunctivitis and asthma in sensitised patients. Much of what we know about the allergen components of grasses is informed by research on pollen of temperate (Pooideae) species that are abundant in the temperate clim...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2018.03.012
更新日期：2018-08-01 00:00:00
abstract：:The binding of CD40 ligand on activated T cells to CD40 on resting B cells induces the expression of costimulatory molecules B7-1 (CD80) and B7-2 (CD86). The induction of B7 molecules by CD40 ligand-CD40 interaction represents a critical step in rendering B cells competent for antigen presentation. The CBA/N mouse has...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(96)00005-3
更新日期：1996-04-01 00:00:00
abstract：:Apoptosis can be induced in the human leukemic T-cell line Jurkat when these cells are stimulated by several known T-cell activators. Among the stimuli that activate Jurkat cells, calcium ionophore A23187, phytohemagglutinin and cross-linked monoclonal antibody anti-CD3 are the best inducers of apoptosis. The activato...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00059-n
更新日期：1995-09-01 00:00:00
abstract：:In an attempt to understand the relationship of amino acid sequence to the formation of primary or multiple myeloma-related amyloid (AL amyloid), we have determined the complete amino acid sequence of amyloid protein BAN. This protein belongs to the kappa I immunoglobulin light chain subgroup and has a polypeptide cha...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90173-2
更新日期：1986-01-01 00:00:00
abstract：:The functional importance of various cell membrane bound molecules was studied and compared in the NK cytotoxicity and CTL activity. LFA-1 and CD2 participate in both killing functions, while CD3 and CD8/CD4 as well as MHC class I molecules are involved only in CTL activity. Nevertheless CD2- and beta 2-microglobulin ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90164-1
更新日期：1986-11-01 00:00:00
abstract：:The two N-linked oligosaccharides in native human C9 were deleted by site-specific mutagenesis. This aglycosyl-C9 did not differ from its native form in hemolytic and bactericidal activity. A new N-glycosylation site (K311N/E313T) was introduced into the turn of a helix-turn-helix [HTH] fold that had been postulated t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.01.013
更新日期：2010-04-01 00:00:00
abstract：:Armenian hamsters were immunized with non-liganded, partially liganded or affinity-labeled anti-fluorescein Mab 4-4-20. Seventeen hybridoma producing monoclonal anti-4-4-20 antibodies were characterized from chemically-mediated fusions of immune hamster lymphocytes with murine Sp2/O-Ag14 myeloma cells. Distinct popula...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90125-u
更新日期：1993-08-01 00:00:00
abstract：:Crotoxin (Ctx) is the main lethal component of Crotalus durissus terrificus venom. It is a neurotoxin, composed of two subunits associated by noncovalent interactions, the non-toxic acid subunit (CA), named Crotapotin, and the basic subunit (CB), with phospholipase A2 (PLA2) activity. Employing the SPOT synthesis tech...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.01.012
更新日期：2020-03-01 00:00:00
abstract：:We have recently developed an in vitro culture model enabling the large-scale expansion of switched-memory B lymphocytes, producing a polyclonal human IgG repertoire. Given the importance of glycosylation for the functions of immunoglobulins, we analyzed the N-glycosylation profiles of the immunoglobulin G (IgG) in th...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2013.10.005
更新日期：2014-02-01 00:00:00
abstract：:To investigate the interaction of DNA and anti-DNA antibodies in the immune complex disease of systemic lupus erythematosus, the fine specificity of binding of a monoclonal anti-DNA antibody was determined. This antibody, termed Cll, was derived from the fusion of spleen cells from an autoimmune MRL-lpr/lpr mouse with...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90364-9
更新日期：1982-05-01 00:00:00
abstract：:Jackfruit lectin, jacalin, prepared from two batches of jackfruit seeds showed a different specificity in precipitating reaction in Agarose gel with various purified immunoglobulins and secretory components. Jacalin-P, extracted from jackfruit seeds from the Philippines, reacts only with serum IgA and secretory IgA of...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90169-6
更新日期：1987-11-01 00:00:00
abstract：:Induction of the TCR signaling pathway terminates the expression of RAG genes, and a link between this pathway and their transcriptional control is evident from the recent demonstration of their re-expression if the TCR is subsequently lost or down-regulated. Since unstimulated T cells display a steady-state level of ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.02.015
更新日期：2011-07-01 00:00:00
