Abstract：
:To understand the mechanism of action of the chaperone protein tapasin, which mediates loading of high-affinity peptides onto major histocompatibility complex (MHC) class I molecules in the antiviral immune response, we have performed numerical simulations of the class I-peptide binding process with four different mechanistic hypotheses from the literature, and tested our predictions by laboratory experiments. We find - in agreement of experimental and theoretical studies - that class I-peptide binding in cells is generally under kinetic control, and that tapasin introduces partial thermodynamic control to the process by competing with peptide for binding to class I. Based on our results, we suggest further experimental directions.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Schneeweiss C,Garstka M,Smith J,Hütt MT,Springer Sdoi
10.1016/j.molimm.2009.02.032subject
Has Abstractpub_date
2009-06-01 00:00:00pages
2054-63issue
10eissn
0161-5890issn
1872-9142pii
S0161-5890(09)00108-4journal_volume
46pub_type
杂志文章abstract：:When 5-fluorouracil (5-FU) resistant bone marrow (BM) cells are depleted of B-cells and then cultured in insert chambers [separated from a layer of adherent BM (aBM) cells by a nucleopore membrane], no mature, lipopolysaccharide (LPS) reactive B-cells are formed. Factors acting on B-cell precursors are not produced un...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90146-0
更新日期：1988-11-01 00:00:00
abstract：:The functional importance of various cell membrane bound molecules was studied and compared in the NK cytotoxicity and CTL activity. LFA-1 and CD2 participate in both killing functions, while CD3 and CD8/CD4 as well as MHC class I molecules are involved only in CTL activity. Nevertheless CD2- and beta 2-microglobulin ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90164-1
更新日期：1986-11-01 00:00:00
abstract：:Despite the fact that receptors for complement on T-cells have been described many years ago the function remains unclear as is the role of complement in the T-cell response. In this review we will evaluate how the accumulated wisdom concur with the current concepts of the adaptive T-cell response. ...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2005.06.027
更新日期：2006-01-01 00:00:00
abstract：:CD9 is a glycoprotein of the transmembrane 4 superfamily (TM4SF) and is involved in various cellular processes. In this study, we describe the isolation of the full-length cDNA encoding for CD9 molecule (daCD9) of red stingray, Dasyatis akajei. This 1252 bp cDNA was isolated from leukocyte cDNA library and contains 68...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.10.005
更新日期：2006-04-01 00:00:00
abstract：:Selection of specific antibodies from large repertoires is of importance in generating antibodies to specific structural determinants and in studying structure-function relationships. Alkaline phosphatase (AP) has several isozymes with various degrees of homology and a range of common synthetic substrates. We have pre...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.02.024
更新日期：2007-01-01 00:00:00
abstract：:The present study reports the characterization of Ls-Stylicin1, a novel antimicrobial peptide from the penaeid shrimp, Litopenaeus stylirostris. The predicted mature peptide of 82 residues is negatively charged (theoretical pI=5.0) and characterized by a proline-rich N-terminal region and a C-terminal region containin...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.12.007
更新日期：2010-03-01 00:00:00
abstract：:MHC class I molecules present short peptides, usually 8-10 amino acids in length, to CD8(+) T cells. These peptides are typically generated from full-length endogenously synthesized proteins degraded by the antigen processing machinery of the target cell. However, exogenous proteins, whether originating from intracell...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.10.002
更新日期：2011-01-01 00:00:00
abstract：:The effectiveness of immunotherapy targeting the immune checkpoint PD-L1/PD-1 pathway highlights importance of elucidating the regulatory mechanisms of PD-L1 expression in cancer cells. Previous studies demonstrate that oncogene MYC up-regulates PD-L1 expression in lymphomas. In the present study, we investigated the ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2018.07.006
更新日期：2018-09-01 00:00:00
abstract：:The integral membrane protein flavocytochrome b (Cyt b) is the catalytic core of the NADPH oxidase complex, a multicomponent enzyme system that initiates a cascade of reactive oxygen species that play a critical role in innate immunity and vascular physiology. Epitope-mapped, monoclonal antibodies (mAb) that recognize...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.10.022
更新日期：2007-01-01 00:00:00
abstract：:We considered the possibility that the greater the distance between an immune receptor V and J, the more likely the V usage. Such a hypothesis is supported by results from mouse experiments. And, such a hypothesis is consistent with the fundamental nature of recombination and genomic distance: the further the distance...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.10.010
更新日期：2019-12-01 00:00:00
abstract：:Conserved extended haplotypes (CEHs) are large (>or=1Mb) regions of identical DNA of the major histocompatibility complex (MHC) region of chromosome 6p in unrelated individuals. They are recognized by family studies and constitute nearly half of MHC haplotypes among European Caucasians. We studied 49 Hungarian Caucasi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.03.013
更新日期：2010-06-01 00:00:00
abstract：:Armenian hamsters were immunized with non-liganded, partially liganded or affinity-labeled anti-fluorescein Mab 4-4-20. Seventeen hybridoma producing monoclonal anti-4-4-20 antibodies were characterized from chemically-mediated fusions of immune hamster lymphocytes with murine Sp2/O-Ag14 myeloma cells. Distinct popula...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90125-u
更新日期：1993-08-01 00:00:00
abstract：:Dendritic cells (DC) survey epithelial or mucosal surfaces for antigens, take them up via their endocytic or phagocytic receptors, process the captured antigens and migrate to the lymphatic tissues. In the draining lymph nodes they present the immunogenic peptides to T cells thereby inducing antigen-specific immune re...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2004.06.019
更新日期：2005-02-01 00:00:00
abstract：:Most cells are believed to be capable of producing type I interferons (IFN I) as part of an innate immune response against, for instance, viral infections. In macrophages, IFN I is potently induced upon cytoplasmic exposure to foreign nucleic acids. Infection of these cells with herpesviruses leads to triggering of th...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.08.025
更新日期：2017-11-01 00:00:00
abstract：:Humanization of monoclonal antibodies by complementary determinant region (CDR)-grafting has become a standard procedure to improve the clinical usage of animal antibodies. However, antibody humanization may result in loss of activity that has been attributed to structural constraints in the framework structure. In th...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(03)00022-1
更新日期：2003-05-01 00:00:00
abstract：:Vitamin D has been recommended as an immune modulator in recent years, in addition to regulating calcium-phosphorous-bone metabolism. Clinical studies on organ transplantation found that vitamin D sufficiency patients were less likely to develop acute cellular rejection within one year after transplantation compared t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.09.006
更新日期：2017-11-01 00:00:00
abstract：:Bovine and equine factor D were purified to apparent homogeneity as evidenced by a single protein staining band on 7.5-17.5% SDS-PAGE slab gels under both reducing and non-reducing conditions. An apparent mol. wt of 15,000 for bovine D and 22,500 for equine D were noted after SDS-PAGE gel analysis of both reduced and ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(84)90141-x
更新日期：1984-10-01 00:00:00
abstract：:A sheep cDNA library was screened with a human C mu probe, and the complete nucleotide sequence of a 1923 nt cDNA was determined. It contains sequences corresponding to all the exons (VH, DH, JH, CH1, CH2, CH3 and CH4) characteristic of the immunoglobulin mu heavy chain regions. The deduced amino acid sequence shows a...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90120-m
更新日期：1992-07-01 00:00:00
abstract：:Symmetry in antibody plus complement mediated killing between a T15 idiotype-bearing monoclonal antibody (HPC-M2) and an anti-T15 monoclonal antibody (B36-82) is demonstrated. The HPC-M2 antibody is also shown to be multispecific in its effector function, since it lyses both phosphorylcholine coated erythrocytes, and ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90083-4
更新日期：1983-08-01 00:00:00
abstract：:Invertebrate cells lack the p53 recombination checkpoint but contain mobile DNA sequences that transpose by a mechanism in part shared with excision of the V(D)J recombination signal sequences (RSS). In this work, inversion, deletion, and duplication of sequences associated with an invertebrate C. elegans Tc6 element ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(99)00034-6
更新日期：1999-05-01 00:00:00
abstract：:A channel catfish genomic lambda library was screened with CH and JH probes which were derived from our earlier sequence analyses on different full-length heavy chain cDNA clones. One clone, designated C7, contained a genomic insert of about 18 kb and hybridized with specific probes for each of the four domains of the...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90096-g
更新日期：1992-02-01 00:00:00
abstract：:Neutrophil migration is essential for battling against infections but also drives chronic inflammation. Since primary neutrophils are terminally differentiated and not genetically tractable, leukemia cells such as HL-60 are differentiated into neutrophil-like cells to study mechanisms underlying neutrophil migration. ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.06.001
更新日期：2019-08-01 00:00:00
abstract：:White spot syndrome virus (WSSV) has caused great economic damage to shrimp aquaculture. Previous studies have shown that WSSV successfully usurps the immunity system of the host for its own gene regulation. To investigate the role of shrimp high mobility group box (HMGB) proteins in WSSV gene regulation, two Litopena...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.11.020
更新日期：2011-02-01 00:00:00
abstract：:MiRNAs are a class of endogenous tiny RNAs that act as inhibitors of translation or promote RNA degradation by duplex-formation within the 3'-UTR of target mRNAs. They play an important role during a wide range of cellular processes by fine-tuning of gene expression. The differentiation of monocytes to macrophages pla...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2013.06.014
更新日期：2013-12-01 00:00:00
abstract：:In humans, three distinct but closely related classes of receptors that bind the Fc portion of IgG (FcgammaRI, II and III) have been identified. FcgammaRI can bind monomeric IgG with high affinity and has a unique third extracellular domain (EC3). Three very similar genes have been characterized for FcgammaRI (A, B, C...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(98)00079-0
更新日期：1998-10-01 00:00:00
abstract：:In periodontal disease, IgG1 and IgA1 antibodies produced in situ deposit on antigens in the affected tissues. Thus, there is an interest in the effect of co-deposited IgA1 antibodies on complement activation by IgG1-immune complexes. In the present study, we first analyzed the effect of IgA1-immune complexes on compl...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.02.004
更新日期：2006-02-01 00:00:00
abstract：BACKGROUND:Individuals genetically deficient of properdin are more susceptible to meningococcal disease. Likewise low concentration or decreased biological activity of mannose-binding lectin (MBL) is associated with higher incidence of bacterial infections during childhood. In this study we report our findings in a Dan...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.02.017
更新日期：2006-02-01 00:00:00
abstract：:The potency of a T cell is determined in large part by two interactions, binding of a cognate peptide to the MHC, and binding of the T cell receptor (TCR) to this pepMHC. Various studies have attempted to assess the relative importance of these interactions, and to correlate the corresponding binding parameters with t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.06.012
更新日期：2009-09-01 00:00:00
abstract：:Low molecular weight hemorrhagins were purified from crude Bothrops atrox snake venom by gel filtration followed by ionic strength chromatography. The protein fractions obtained, designated HI-1 to HI-8, contained proteins with molecular masses lower than 30 kDa. HI-5, the most representative among of these fractions,...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2003.11.032
更新日期：2004-03-01 00:00:00
abstract：:We have previously identified four linear IgE epitopes on Jun a 1, the dominant allergen in mountain cedar pollen, and mapped these to the surfaces of a molecular model and to the crystal structure of this glycoprotein. The aim of the present study was to determine if Jun a 1 also displays conformational IgE epitopes....
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.12.001
更新日期：2007-04-01 00:00:00
